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Design and methods of the U.S. Geological Survey Northeast Stream Quality Assessment (NESQA), 2016

During 2016, as part of the National Water-Quality Assessment Project (NAWQA), the U.S. Geological Survey conducted the Northeast Stream Quality Assessment (NESQA) to investigate stream quality in the northeastern United States. The goal of the NESQA was to assess the health of wadeable streams in the region by characterizing multiple water-quality factors that are stressors to aquatic life and by evaluating the relation between these stressors and the condition of biological communities. Urbanization, agriculture, and human modifications to streamflow are anthropogenic changes that greatly affect water quality in the region; consequently, the study design primarily selected sites and targeted stressors associated with these activities. The NESQA built on a prior NAWQA study conducted in the region in 2014, the Atlantic Highlands flow-ecology study, which investigated the effects of anthropogenically modified flows on aquatic biological communities in primarily forested watersheds. Land-cover data for the NESQA were used to identify and select sites within the region that had watersheds ranging in levels of urban and agricultural development. A total of 95 sites were selected: 67 on streams in watersheds representing a range of urban land use, 13 on streams in watersheds with some degree of agricultural land use, and 15 on streams in predominantly forested watersheds with little development. Depending on land-cover characteristics, sites were sampled weekly for metal and organic contaminants, nutrients, and sediment for either a 9-week period that began the week of June 6, 2016, or a 4-week period that begin the week of July 11, 2016. Beginning August 1, 2016, and for about 2 weeks, an ecological survey was conducted at every site to assess stream habitat, and algal, benthic invertebrate, and fish communities. Additional samples collected during the ecological surveys were streambed sediment for chemical analysis and toxicity testing, and fish tissue for mercury analysis. This report describes the various study components and methods of the NESQA and describes a precursor effort for the Atlantic Highlands flow-ecology study. Details are presented for measurements of water quality, sediment chemistry, streamflow, and ecological surveys of stream biota and habitat, as well as processes of sample analysis, quality assurance and quality control, and data management.

Open-File Report

Fish growth rates and lake sulphate explain variation in mercury levels in ninespine stickleback (Pungitius pungitius) on the Arctic Coastal Plain of Alaska

Mercury concentrations in freshwater food webs are governed by complex biogeochemical and ecological interactions that spatially vary and are often mediated by climate. The Arctic Coastal Plain of Alaska (ACP) is a heterogeneous, lake-rich landscape where variability in mercury accumulation is poorly understood. Earlier research indicated that the level of catchment influence on lakes varied spatially on the ACP, and affected mercury accumulation in lake sediments. This work sought to determine drivers of spatial variation in mercury accumulation in lake food webs on the ACP. Three lakes that were a priori identified as “high catchment influence” (Reindeer Camp region) and three lakes that were a priori identified as “low catchment influence” (Atqasuk region) were sampled, and variability in water chemistry, food web ecology, and mercury accumulation was investigated. Among-lake differences in ninespine stickleback ( Pungitius pungitius ) length-adjusted methylmercury concentrations were significantly explained by sulphate concentration in lake water, a tracer of catchment runoff input. This effect was mediated by fish growth, which had no pattern between regions. Together, lake water sulphate concentration and fish age-at-size (proxy for growth) accounted for nearly all of the among-lake variability in length-adjusted methylmercury concentrations in stickleback (R 2 adj = 0.94, p < 0.01). The percentage of total mercury as methylmercury (a proxy for net Hg methylation) was higher in sediments of more autochthonous, “low catchment influence” lakes ( p < 0.05), and in the periphyton of more allochthonous, “high catchment influence” lakes (p < 0.05). The results indicate that dominant sources of primary production (littoral macrophyte/biofilm vs. pelagic phytoplankton) and food web structure (detrital vs. grazing) are regulated by catchment characteristics on the ACP, and that this ultimately influences the amount of methylmercury in the aquatic food web. These results have important implications for predicting future mercury concentrations in fish in lakes where fish growth rates and catchment inputs may change in response to a changing climate.

Alaska

Differences in native soil ecology associated with invasion of the exotic annual chenopod, Halgeton glomeratus

Various biotic and abiotic components of soil ecology differed significantly across an area where Halogeton glomeratus is invading a native winterfat, [ Krascheninnikovia (= Ceratoides ) lanata ] community. Nutrient levels were significantly different among the native, ecotone, and exotic-derived soils. NO 3 , P, K, and Na all increased as the cover of halogeton increased. Only Ca was highest in the winterfat area. A principal components analysis, conducted separately for water-soluble and exchangeable cations, revealed clear separation between halogeton- and winterfat-derived soils. The diversity of soil bacteria was highest in the exotic, intermediate in the ecotone, and lowest in the native community. Although further studies are necessary, our results offer evidence that invasion by halogeton alters soil chemistry and soil ecology, possibly creating conditions that favor halogeton over native plants.

Biology and Fertility of Soils

Rivers of Arctic North America

This chapter describes the geomorphology, hydrology, chemistry, biodiversity, and ecology of rivers in the North American Arctic. The history, physiography, climate, and land use of the Arctic regions are also described. The chapter includes details on the Kobuk and Colville rivers in Alaska, the Thelon and Kazan rivers in the central Canadian Arctic, Koroc River and Nakvak Brook in the eastern Canadian low Arctic, Thomsen River on Banks Island in the western Canadian Arctic Archipelago, and Ruggles River on Ellesmere Island in the Canadian high Arctic. The rivers are characteristic of the major ecoregions of the North American Arctic, covering a range of geomorphological and physiographic conditions. The history of use of the rivers by Inuit and Dene First Nations Peoples of the north provides the foundation to understand the social, cultural, and economic importance of the river systems, and potential threats to the rivers from climate change are outlined.

Book chapter

Handbook of ecotoxicology, second edition

Handbook of Ecotoxicology, Second Edition focuses on toxic substances and how they affect ecosystems worldwide. It presents methods for quantifying and measuring ecotoxicological effects in the field and in the lab, as well as methods for estimating, predicting, and modeling in ecotoxicology studies. Completely revised and updated with 18 new chapters, this second edition includes contributions from over 75 international experts. Also, a Technical Review Board reviewed all manuscripts for accuracy and currency. This authoritative work is the definitive reference for students, researchers, consultants, and other professionals in the environmental sciences, toxicology, chemistry, biology, and ecology - in academia, industry, and government.

Book

The benthic foraminifera cassidulina from the Arctic Ocean: Application to paleoceanography and biostratigraphy

We investigated the morphology, biostratigraphy, shell stable isotope composition and paleogeography of the common Arctic benthic foraminifera, Cassidulina teretis (Tappan 1951) (sometimes assigned to Islandiella (Nørvang 1958), for application to Quaternary paleoceanography. Cassidulina teretis, which has been studied by several generations of Arctic foraminiferal specialists, is used in Arctic Ocean paleoceanographic reconstructions based on foraminiferal assemblages and, increasingly, isotope shell chemistry. Here we review its modern and fossil distribution including discussions of its taxonomy, ecology, biostratigraphy and shell chemistry. Cassidulina teretis Tappan 1951, originally described from the Gubik Formation, northern Alaska coastal plain, has variability in test size, apertural morphology and development of an umbilical boss representing intra- and inter-population differences across the Arctic and subarctic in modern, Quaternary and Pliocene assemblages. Nonetheless, our studies and those previously published lead us to conclude that populations from the Arctic Ocean represent a single species proposed by Tappan as Cassidulina teretis. Its modern distribution is mainly 200 to 1000 m water depth, often living within the core of the relatively warm Atlantic Layer. However, shallower occurrences suggest other factors, such as food supply, are also critical to its ecology. The Holocene distribution of Cassidulina teretis in the Beaufort Sea boundary indicate millennial-scale changes in relative abundance related to changing Atlantic Layer influence, sea-ice cover, surface productivity and food availability. There are extremely large changes in its abundance during the last deglacial interval on the Yermak Plateau, Barents Sea slope and the Laptev Sea reflecting rapid ocean changes during the Bølling-Allerød, Younger Dryas, and Preboreal. Similarly, C. teretis abundance changes during the last 300,000 years allow us to use it, at least regionally, as a useful biostratigraphic marker. The stable isotopic composition of Cassidulina teretis tests holds promise for establishing an isotope stratigraphy across the Arctic Ocean and perhaps also in the Nordic Seas, off Iceland and in the northern North Atlantic Ocean, once disequilibrium values and offsets from other Arctic benthic species are more firmly established.

Micropaleontology

Design and methods of the California stream quality assessment (CSQA), 2017

During 2017, as part of the National Water-Quality Assessment Project, the U.S. Geological Survey conducted the California Stream Quality Assessment to investigate the quality of streams in the Central California Foothills and Coastal Mountains ecoregion, United States. The goal of the California Stream Quality Assessment study was to assess the health of wadeable streams in the region by characterizing multiple water-quality factors that are stressors to aquatic biota and by evaluating the relation between these stressors and biological indicators of stream health. Urbanization, agriculture, and modifications to streamflow are anthropogenic changes that affect water quality in the region; consequently, the study design primarily targeted sites and specific stressors associated with these activities. For the study, 85 stream sites were selected to represent the types and intensity of land use in the watershed; categories of site types were undeveloped, urban (low, medium, high), agriculture (low, high), and mixed (urban and agriculture). Most sites (about 70 percent) represent a gradient of urbanization from undeveloped to 99-percent urbanized. At most of the sites, streamgages or pressure transducers were used to monitor stream discharge and stage, as well as temperature. Water-quality samples were collected routinely at all sites and were analyzed for major ions, organic contaminants, nutrients, and suspended sediment. Sampling frequency varied on the basis of site type and location. Discrete water samples were collected weekly and generally 6 times per site, except for 11 undeveloped sites that were sampled only 4 times (during the last 4 weeks). Water sampling began at sites in the southern part of the study on March 13, 2017, and at sites in the northern part of the study on April 3, 2017. Passive samplers were deployed at most sites for measurement of polar organic contaminants (pesticides and pharmaceuticals). In May 2017, coincident with completion of water-quality sampling, an ecological survey was conducted at each site to assess benthic algal and macroinvertebrate communities and instream habitat. During the ecological surveys, a single composite streambed-sediment sample was collected for chemical analysis and toxicity testing. In addition, a few focused studies were done at subsets of sites, namely, measuring pesticides using small-volume automated samplers, measuring pesticides in biofilms, and sampling suspended sediments using passive samplers. This report describes the various study components and methods of the California Stream Quality Assessment, including measurements of water quality, sediment chemistry, habitat assessments, and ecological surveys, as well as procedures for sample analysis, quality assurance and quality control, and data management.

California

Diazinon residues in insects from sprayed tobacco

Pooled samples of tobacco hornworms collected from a field sprayed with 0.84 kg/ha of diazinon were analyzed for residues at various intervals after application. No residues of the toxic metabolite diazoxon were detected (sensitivity 0.5 ppm) in any sample. Only one sample exceeded 1.0 ppm of the parent compound and was collected 4 hours after spraying. Residues declined over time (P<0.01) and none were detected (sensitivity 0.1 ppm) 18 days after spraying. the potential hazard to birds eating these insects appeared to be minimal.

Maryland

Organophosphate residues in grasshoppers from sprayed rangelands

Grasshoppers (Orthoptera) were collected in pastures that had been sprayed with malathion and acephate to estimate the secondary exposure of insectivorous birds to those pesticides. Residues of malathion were below 3 ppm at 30 and 54 hours after spraying and no malaoxon was detected. In contrast, acephate was found at 8 and 9 ppm 4 hours after spray; 3–5 ppm of the toxic metabolite methamidophos were also detected at that time. By 53 hours postspray, acephate levels declined to 2 ppm and methamidophos to less than 1 ppm. These results suggest that although malathion may not be a hazard to insectivorous species, acephate may be hazardous through metabolic transformation to methamidophos.

Chemistry and Ecology

Columbia Environmental Research Center

The U.S. Geological Survey Columbia Environmental Research Center performs research to solve challenging environmental problems related to contaminants and habitat alterations in aquatic and terrestrial ecosystems. The research is interdisciplinary and pursued through partnerships within the U.S. Geological Survey and with national, international, state, and local agencies; nongovernmental organizations; and universities. Research is prioritized to provide science to the U.S. Department of the Interior and other natural resource management agencies to inform rehabilitation of degraded habitats and imperiled fish and wildlife populations. The Columbia Environmental Research Center was established in 1966 in Columbia, Missouri, as the U.S. Fish and Wildlife Service’s Fish Pesticide Research Laboratory; the Columbia Environmental Research Center was incorporated into the U.S. Geological Survey in 1996. The U.S. Geological Survey’s staff of 130 includes 90 scientists of which one-half have advanced degrees in ecology, toxicology, biology, biochemistry, chemistry, hydrology, geology, and information technology.

Missouri

Variability of the carbonate chemistry in a shallow, seagrass-dominated ecosystem: implications for ocean acidification experiments

Open ocean observations have shown that increasing levels of anthropogenically derived atmospheric CO 2 are causing acidification of the world's oceans. Yet little is known about coastal acidification and studies are just beginning to characterise the carbonate chemistry of shallow, nearshore zones where many ecologically and economically important organisms occur. We characterised the carbonate chemistry of seawater within an area dominated by seagrass beds (Saint Joseph Bay, Florida) to determine the extent of variation in pH and p CO 2 over monthly and daily timescales. Distinct diel and seasonal fluctuations were observed at daily and monthly timescales respectively, indicating the influence of photosynthetic and respiratory processes on the local carbonate chemistry. Over the course of a year, the range in monthly values of pH (7.36-8.28), aragonite saturation state (0.65-5.63), and calculated pCO2 (195-2537 &mu;atm) were significant. When sampled on a daily basis the range in pH (7.70-8.06), aragonite saturation state (1.86-3.85), and calculated p CO 2 (379-1019 &mu;atm) also exhibited significant range and indicated variation between timescales. The results of this study have significant implications for the design of ocean acidification experiments where nearshore species are utilised and indicate that coastal species are experiencing far greater fluctuations in carbonate chemistry than previously thought.

Marine and Freshwater Research

A guide for establishing restoration goals for contaminated ecosystems

As natural resources become increasingly limited, the value of restoring contaminated sites, both terrestrial and aquatic, becomes increasingly apparent. Traditionally, goals for remediation have been set before any consideration of goals for ecological restoration. The goals for remediation have focused on removing or limiting contamination whereas restoration goals have targeted the ultimate end use. Here, we present a framework for developing a comprehensive set of achievable goals for ecological restoration of contaminated sites to be used in concert with determining goals for remediation. This framework was developed during a Society of Environmental Toxicology and Chemistry (SETAC) and Society of Ecological Restoration (SER) cosponsored workshop that brought together experts from multiple countries. Although most members were from North America, this framework is designed for use internationally. We discuss the integration of establishing goals for both contaminant remediation and overall restoration, and the need to include both the restoration of ecological and socio-cultural-economic value in the context of contaminated sites. Although recognizing that in some countries there may be regulatory issues associated with contaminants and clean up, landscape setting and social drivers can inform the restoration goals. We provide a decision tree support tool to guide the establishment of restoration goals for contaminated ecosystems. The overall intent of this decision tree is to provide a framework for goal setting and to identify outcomes achievable given the contamination present at a site.

Integrated Environmental Assessment and Management

Rivers of the Lower Mississippi Basin

Discussed in this chapter are seven significant tributaries of the Lower Mississippi River and its major distributary. As a group, these eight rivers and their basins encompass substantial variation in physical form, hydrology, biota, ecology, and human impacts. The Current River, Ouachita River, and Saline River, flow to the Mississippi out of the U.S. Interior Highlands. The Cache River basin, centered in Arkansas, contains a vast expanse of bottomland hardwood forest and is famous for wintering waterfowl. The Hatchie River, flowing west out of Tennessee, is the longest free-flowing tributary of the Lower Mississippi River and famous for its rich diversity of mussels and fishes. The Wolf River of Tennessee supports a magnificent bald cypress-tupelo swamp and is notable as a protected urban river that flows through Memphis. The Big Sunflower River begins and ends in the alluvial floodplain of the Mississippi River and flows through a basin of intense agriculture and historical human conflict. The Atchafalaya River, the primary distributary of the Mississippi River, supports the nation's largest expanse of bottomland hardwood forest and swamp wetlands. In this chapter, we review the physiography, geomorphology, hydrology, water chemistry, land use, biological diversity, ecological processes, human impacts, and areas of need for research and management of each of these eight rivers and their basins.

Lower Mississippi River basin

Restoration of impaired ecosystems: An ounce of prevention or a pound of cure? introduction, overview, and key messages from a SETAC-SER workshop

A workshop on Restoration of Impaired Ecosystems was held in Jackson, Wyoming, in June 2014. Experts from Australia, Canada, Mexico, the United Kingdom, and the United States in ecotoxicology, restoration, and related fields from both the Society of Environmental Toxicology and Chemistry and the Society for Ecological Restoration convened to advance the practice of restoring ecosystems that have been contaminated or impaired from industrial activities. The overall goal of this workshop was to provide a forum for ecotoxicologists and restoration ecologists to define the best scientific practices to achieve ecological restoration while addressing contaminant concerns. To meet this goal, participants addressed 5 areas: 1) links between ecological risk assessment and ecological restoration, 2) restoration goals, 3) restoration design, 4) monitoring for restoration effectiveness and 5) recognizing opportunities and challenges. Definitions are provided to establish a common language across the varied disciplines. The current practice for addressing restoration of impaired ecosystems tends to be done sequentially to remediate contaminants, then to restore ecological structure and function. A better approach would anticipate or plan for restoration throughout the process. By bringing goals to the forefront, we may avoid intrusive remediation activities that close off options for the desired restoration. Participants realized that perceived limitations in the site assessment process hinder consideration of restoration goals; contaminant presence will influence restoration goal choices; social, economic, and cultural concerns can factor into goal setting; restoration options and design should be considered early during site assessment and management; restoration of both structure and function is encouraged; creative solutions can overcome limitations; a regional focus is imperative; monitoring must occur throughout the restoration process; and reciprocal transfer of knowledge is needed among theorists, practitioners, and stakeholders and among varied disciplines.

Integrated Environmental Assessment and Management

Evaluating pharmaceuticals and other organic contaminants in the Lac du Flambeau Chain of Lakes using risk-based screening techniques

In an investigation of pharmaceutical contamination in the Lac du Flambeau Chain of Lakes (hereafter referred to as “the Chain”), few contaminants were detected; only eight pharmaceuticals and one pesticide were identified among the 110 pharmaceuticals and other organic contaminants monitored in surface water samples. This study, conducted in cooperation with the Lac du Flambeau Tribe’s Water Resource Program, investigated these organic contaminants and potential biological effects in channels connecting lakes throughout the Chain, including the Moss Lake Outlet site, adjacent to the wastewater treatment plant lagoon. Of the 6 sites monitored and 24 samples analyzed, sample concentrations and contaminant detection frequencies were greatest at the Moss Lake Outlet site; however, the concentrations and detection frequencies of this study were comparable to other pharmaceutical investigations in basins with similar characteristics. Because established water-quality benchmarks do not exist for the pharmaceuticals detected in this study, alternative screening-level water-quality benchmarks, developed using two U.S. Environmental Protection Agency toxicological resources (ToxCast database and ECOTOX knowledgebase), were used to estimate potential biological effects associated with the observed contaminant concentrations. Two contaminants (caffeine and thiabendazole) exceeded the prioritization threshold according to ToxCast alternative benchmarks, and four contaminants (acetaminophen, atrazine, caffeine, and carbamazepine) exceeded the prioritization threshold according to ECOTOX alternative benchmarks. Atrazine, an herbicide, was the most frequently detected contaminant (79% of samples), and it exhibited the strongest potential for biological effects due to its high estimated potency. Insufficient toxicological information within ToxCast and ECOTOX for gabapentin and methocarbamol (which had the two greatest concentrations in this study) precluded alternative benchmark development. This data gap presents unknown potential environmental impacts. Future research examining the biological effects elicited by these two contaminants as well as the others detected in this study would further elucidate the ecological relevance of the water chemistry results generated though this investigation.

Wisconsin

Application of a multipurpose unequal probability stream survey in the Mid-Atlantic Coastal Plain

A stratified, spatially balanced sample with unequal probability selection was used to design a multipurpose survey of headwater streams in the Mid-Atlantic Coastal Plain. Objectives for the survey include unbiased estimates of regional stream conditions, and adequate coverage of unusual but significant environmental settings to support empirical modeling of the factors affecting those conditions. The design and field application of the survey are discussed in light of these multiple objectives. A probability (random) sample of 175 first-order nontidal streams was selected for synoptic sampling of water chemistry and benthic and riparian ecology during late winter and spring 2000. Twenty-five streams were selected within each of seven hydrogeologic subregions (strata) that were delineated on the basis of physiography and surficial geology. In each subregion, unequal inclusion probabilities were used to provide an approximately even distribution of streams along a gradient of forested to developed (agricultural or urban) land in the contributing watershed. Alternate streams were also selected. Alternates were included in groups of five in each subregion when field reconnaissance demonstrated that primary streams were inaccessible or otherwise unusable. Despite the rejection and replacement of a considerable number of primary streams during reconnaissance (up to 40 percent in one subregion), the desired land use distribution was maintained within each hydrogeologic subregion without sacrificing the probabilistic design.

Journal of the American Water Resources Associatio

Design and methods of the Southeast Stream Quality Assessment (SESQA), 2014

During 2014, the U.S. Geological Survey (USGS) National Water-Quality Assessment Program (NAWQA) assessed stream quality across the Piedmont and southern Appalachian Mountain regions of the southeastern United States. This Southeast Stream Quality Assessment (SESQA) simultaneously characterized watershed and stream-reach water-quality stressors along with instream biological conditions, in order to better understand regional stressor-effects relations. The goal of SESQA is to provide communities and policymakers with information about those human and environmental factors that have the greatest impact on stream quality across the region. The SESQA design focused on hydrologic alteration and urbanization because of their importance as ecological stressors of particular concern to Southeast region resource managers. Streamflow and land-use data were used to identify and select sites representing gradients in urbanization and streamflow alteration across the region. One hundred fifteen sites were selected and sampled for as many as 10 weeks during April, May, and June 2014 for contaminants, nutrients, and sediment. This water-quality &ldquo;index&rdquo; period culminated with an ecological survey of habitat, periphyton, benthic macroinvertebrates, and fish at all sites. Sediment was collected during the ecological survey for analysis of sediment chemistry and toxicity testing. Of the 115 sites, 59 were on streams in watersheds with varying degrees of urban land use, 5 were on streams with multiple confined animal feeding operations, and 13 were reference sites with little or no development in their watersheds. The remaining 38 &ldquo;hydro&rdquo; sites were on streams in watersheds with relatively little agricultural or urban development but with hydrologic alteration, such as a dam or reservoir. This report provides a detailed description of the SESQA study components, including surveys of ecological conditions, routine water sampling, deployment of passive polar organic compound integrative samplers for pesticides and contaminants of emerging concern, and synoptic sediment sampling and toxicity testing at all urban, confined animal feeding operation, and reference sites. Continuous water-quality monitoring and daily pesticide sampling efforts conducted at a subset of urban sites are also described.

Alabama, Georgia, North Carolina, South Carolina,

Significance of headwater streams and perennial springs in ecological monitoring in Shenandoah National Park

Shenandoah National Park has been monitoring water chemistry and benthic macroinvertebrates in stream ecosystems since 1979. These monitoring efforts were designed to assess the status and trends in stream condition associated with atmospheric deposition (acid rain) and changes in forest health due to gypsy moth infestations. The primary objective of the present research was to determine whether the current long-term macroinvertebrate and water-quality monitoring program in Shenandoah National Park was failing to capture important information on the status and trends in stream condition by not sufficiently representing smaller, headwater streams. The current benthic-macroinvertebrate and water-chemistry sampling designs do not include routine collection of data from streams with contributing watershed areas smaller than 100 hectares, even though these small streams represent the overwhelming proportion of total stream length in the park. In this study, we sampled headwater sites, including headwater stream reaches (contributing watershed area approximately 100 hectares (ha) and perennial springs, in the park for aquatic macroinvertebrates and water chemistry and compared the results with current and historical data collected at long-term ecological monitoring (LTEM) sites on larger streams routinely sampled as part of ongoing monitoring efforts. The larger purpose of the study was to inform ongoing efforts by park managers to evaluate the effectiveness and efficiency of the current aquatic monitoring program in light of other potential stressors (for example, climate change) and limited resources. Our results revealed several important findings that could influence management decisions regarding long-term monitoring of park streams. First, we found that biological indicators of stream condition at headwater sites and perennial springs generally were more indicative of lower habitat quality and were more spatially variable than those observed at sites on routinely monitored larger streams. We hypothesized that poorer stream condition observed in smaller streams was due to stream drying that occurs more frequently in headwater areas. We also found that biological and water-chemistry measures responded differently to landscape drivers. Variation in most biological endpoints was driven primarily by stream size and was only secondarily associated with bedrock geology. In contrast, water chemistry showed essentially the opposite pattern, with underlying geology explaining much of the variation and stream size being of secondary importance. Therefore, expanding the LTEM program to include headwater areas would yield substantially different biological information, whereas broad inferences regarding spatial patterns in water chemistry would probably not change. Although significant differences in community composition were observed among streams of different sizes, no taxa were unique to headwater sites. All taxa collected at the 45 headwater sites also had been collected at one or more LTEM sites during one or more years. This observation indicates that headwater sites in the park may be structured by biotic nestedness; consequently, focusing management efforts on preserving the species pool at the larger LTEM sites would likely result in the protection of most taxa parkwide. Finally, linkages (correlations) between water chemistry and biological measures of stream condition were signficantly stronger when assessed at the LTEM sites than when assessed at the springs or headwater sites, indicating that conditions at downstream sites may be better indicators of water-quality trends.

Virginia