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Some considerations in modeling the mallard life cycle

We outline a population model proposed to accommodate the full life cycle of the mallard (Anas platyrhynchos). Events during the breeding season are better understood than events at other times of the year, but recent findings suggest the importance of phenomena away from the breeding grounds. Several processes are discussed relative to mallard population dynamics. Compensatory mortality is a poorly understood concept, but one that can overwhelm many other components of a population model. Diseases and environmental contaminants can inflict indirect as well as direct mortality and can reduce reproduction. They interact with numerous other variables in complex and yet unknown ways. Recent evidence of a wintering-ground effect on subsequent recruitment provides one avenue for modeling phenomena occurring at different times of the year. Finally, the role of heterogeneity among individuals is widely acknowledged but not fully appreciated. We illustrate with an example the importance of heterogeneity to population processes, including compensatory mortality.

Book chapter

Changes in the active, dead, and dormant microbial community structure across a Pleistocene permafrost chronosequence

Permafrost hosts a community of microorganisms that survive and reproduce for millennia despite extreme environmental conditions such as water stress, subzero temperatures, high salinity, and low nutrient availability. Many studies focused on permafrost microbial community composition use DNA-based methods such as metagenomic and 16S rRNA gene sequencing. However, these methods do not distinguish between active, dead, and dormant cells. This is of particular concern in ancient permafrost where constant subzero temperatures preserve DNA from dead organisms and dormancy may be a common survival strategy. To circumvent this we applied: (i) live/dead differential staining coupled with microscopy, (ii) endospore enrichment, and (iii) selective depletion of DNA from dead cells to permafrost microbial communities across a Pleistocene permafrost chronosequence (19 thousand years (K), 27K, and 33K). Cell counts and analysis of 16S rRNA gene amplicons from live, dead, and dormant cells revealed how communities differ between these pools, how they are influenced by soil physicochemical properties, and whether they change over geologic time. We found evidence that cells capable of forming endospores are not necessarily dormant and that members of class Bacilli were more likely to form endospores in response to long-term stressors associated with permafrost environmental conditions than members of Clostridia, which were more likely to persist as vegetative cells in our older samples. We also found that removing exogenous ‘relic’ DNA preserved within permafrost did not significantly alter microbial community composition. These results link the live, dead, and dormant microbial communities to physicochemical characteristics and provides insights into the survival of microbial communities in ancient permafrost.

Alaska

Integrated modeling approach for fate and transport of submerged oil and oil-particle aggregates in a freshwater riverine environment

The Enbridge Line 6B pipeline release of diluted bitumen into the Kalamazoo River downstream of Marshall, Michigan, U.S.A., in July 2010 was one of the largest oil spills into freshwater in North American history. A portion of the oil interacted with river sediment and submerged requiring the development and implementation of new approaches for detection and recovery of oil mixed with river sediment. Hydrodynamic and sediment transport modeling became an integral part of containment and recovery operations for decision support about the potential fate and migration of submerged oil and oiled sediment. Three models were developed for the U.S. Environmental Protection Agency to cover a range of spatial scales of interest to onsite operations. Two-dimensional (2D) hydrodynamic and sediment transport models from the Environmental Fluid Dynamics Code and the sediment bed model SEDZLJ1 were used to simulate potential resuspension, migration, and deposition of submerged oil and oiled sediment along a 38-mile reach of the Kalamazoo River affected by the oil from Marshall to Kalamazoo. An algorithm was added to SEDZLJ to represent three additional particle size classes of oilparticle aggregates (OPAs) with a range of sizes, specific gravities, and settling velocities. Field and laboratory experiments and flume tests were done to support the numerical modeling of OPAs. A three-dimensional hydrodynamic model was developed to simulate hydrodynamics and OPA tracking through Morrow Lake, the most downstream impoundment. This model incorporated wind and dam operations into high and low flow, lake drawdown, and containment simulations. Finally, a 2D unstructured grid model, HydroSed2D, was used to simulate flows and sediment transport along 1- to 2-mile segments of the Kalamazoo River around islands and through side channels and backwater areas that are particularly prone to submerged oil deposition. Integrated models could be developed quickly due to the availability of information and services combined with spill response operations that included: bathymetry and topography data, fieldbased geomorphic mapping of submerged oil, and discharge measured at U.S. Geological Survey streamflow gauges. Modeling results were included in a multiple-lines-of-evidence approach that was used by the Federal On-Scene Coordinator and operations staff for decision-making related to assessment and recovery of submerged oil, as well as net environmental benefit analysis. Similar modeling approaches will likely be useful for future oil spills in riverine environments.

Michigan

Spatial and temporal variation in positioning probability of acoustic telemetry arrays: Fine-scale variability and complex interactions

Background As popularity of positional acoustic telemetry systems increases, so does the need to better understand how they perform in real-world applications, where variation in performance can bias study conclusions. Studies assessing variability in positional telemetry system performance have focused primarily on position accuracy, or comparing performance inside and outside the array. Here, we explored spatial and temporal variation in positioning probability within a 140-receiver Vemco Positioning System (VPS) array used to monitor lake trout, Salvelinus namaycush , spawning behavior over 23 km 2 in Lake Huron, North America. Methods Variability in VPS positioning probability was assessed between August and November from 2012 to 2014 using 43 stationary transmitters distributed throughout the array. Various analyses were used to relate positioning probability to number of fish transmitters in the array, wave height, and thermal stratification. We also assessed the prevalence of ‘close proximity detection interference’ (CPDI) in our array by analyzing detection probability of 35 transmitters on collocated receivers. Results Positioning probability of the VPS array varied greatly over time and space. Number of fish transmitters present in the array was a significant driver of reduced positioning probability, especially during lake trout spawning period when the fish were aggregated. Relationships between positioning probability and environmental variables were complex and varied over small spatial and temporal scales. One possible confounding variable was the large range of water depth over which receivers were deployed. Another confounding factor was the high prevalence of CPDI, which decreased exponentially with water depth and was less evident when wave heights were higher than normal. Conclusions Some variables that negatively influenced positioning can be minimized through careful planning (e.g., number of tagged fish released, transmitter power level). However, results suggested that the acoustic environment was highly variable over small spatial and temporal scales in response to complex interactions between many variables. Therefore, models that predict positioning or detection efficiencies as a function of environmental variables may not be attainable in most systems. The best defense against biased study conclusions is incorporation of in situ measures of system performance that allow for retrospective analysis of array performance after a study is completed. Keywords Vemco Positioning System Positional telemetry Performance Detection probability Close proximity detection interference Thermal stratification Wave height Signal code collision

Animal Biotelemetry

Evidence for exposure to selenium by breeding interior snowy plovers (Charadrius nivosus) in saline systems of the Southern Great Plains

Interior snowy plover ( Charadrius nivosus ) population declines and deteriorating conditions throughout the Southern Great Plains (SGP) of Texas, New Mexico, and Oklahoma may be linked to environmental contaminants. Concentrations of V, As, Cd, Pb, and Se were quantified in breeding snowy plover blood, feathers (5th primary; P5), and potential prey (tiger beetles [ Cicindela circumpicta and C. togata ]). Se was (a) most commonly detected relative to other quantified elements and (b) frequently quantified at levels exceeding background or toxicity thresholds. Of samples greater than instrumentation detection limits, 98% of snowy plover blood and 22% of feather samples were greater than Se toxicity thresholds of 1 ppm ww for blood and 5 ppm dw for feathers (blood quantifiable range: 0.83–15.12 ppm; feathers quantifiable range: 1.90–27.47 ppm). Almost all tiger beetle Se concentrations were below reported invertebrate thresholds of 30 ppm dw (quantifiable range: 0.54–45.84 ppm). Snowy plover blood Se concentrations were related to sex, individual body condition, and local tiger beetle Se concentrations, while plover P5 Se concentrations were related to state, sex, and presence of body molt. Tiger beetle Se concentrations were related to individual study sites in Texas. These results provide some of the first evidence of Se exposure risk for interior snowy plovers nesting in saline lake and alkali flat environments of the SGP. Future efforts should focus upon specific Se uptake pathways during breeding and nonbreeding seasons, as snowy plovers breeding in the SGP appear to be exposed to Se throughout their annual cycle.

New Mexico, Oklahoma, Texas

Benthic invertebrate population characteristics as affected by water quality in coal-bearing regions of Tennessee

Benthic invertebrate and water quality data collected during previous U.S. Geological Survey studies to provide background hydrologic information on streams draining Tennessee coal reserves, were evaluated to identify possible relations between stream biota and water quality. Linear regressions produced low correlation coefficients relating the number of taxa/sample, total number of organisms/sample, sample diversity, and percentage composition of selected orders of invertebrates, with average water quality parameter values available at sampling stations (r is < 0.62 at p=0.05). Analyses of these data by linear regressions explained little of the variability in benthic invertebrate samples primarily because the distributions of benthic organisms along environmental gradients are nonlinear. Variability in substrate characteristics in the study area and seasonal insect emergence patterns also complicated interpretation of these data. However, analysis of variance tests did indicate significant trends towards reduced number of taxa, number of organisms, and sample diversity at stations with relatively poor water quality conditions. Decreasing percentage composition of Ephemeroptera was generally accompanied by an increase in percent Diptera at stations with higher water quality constituent concentrations and acidic pH (> than 0.6 units). These trends indicate significant differences in benthic communities at sites with evidence of more severe land use impacts. Additional data on benthic invertebrates, water quality, and physical habitat conditions, along with analyses of data using multivariate statistical methods are needed to define ecological relations between specific groups of invertebrates and environmental conditions.

Tennessee

Wildlife disease in the Colorado Delta as an indicator of ecosystem health

Ecosystem health and management are relatively new paradigms for environmental management (Costanza et al., 1992) and will be continually redefined and reevaluated relative to their purpose and utility in serving human society (Costanza et al., 1992; Sutter, 1993; Grumbine, 1994; Lackey, 1998, 1999). The stimulus for ecosystem management is often associated with sustaining or restoring ecosystem health to provide values sought by humans (Costanza et al., 1992). We accept the concepts of ecosystem health and management as useful precepts. However, we argue that the orientation in serving human values often has resulted in inadequate attention being given to nonhuman species. The need to address ecosystem health for the benefit of all species that share Planet Earth is noted by Merchant (1997). This humanistic viewpoint also has economic benefits that are part of the wealth that we will pass on to future generations (Pulliam, 1995). Despite the viewpoints just noted, disease occurrence in wildlife receives inadequate consideration with regard to perspectives of ecosystem health and as a focus for ecosystem management. Evidence for this view is the limited resources allocated for wildlife disease programs and prevention efforts, and persistent attitudes within the wildlife conservation community that disease is a “natural event,” not influenced by environmental conditions or anthropogenic insults.

California

Stable metal isotopes reveal copper accumulation and loss dynamics in the freshwater bivalve Corbucula

Characterization of uptake and loss dynamics is critical to understanding risks associated with contaminant exposure in aquatic animals. Dynamics are especially important in addressing questions such as why coexisting species in nature accumulate different levels of a contaminant. Here we manipulated copper (Cu) stable isotopic ratios (as an alternative to radioisotopes) to describe for the first time Cu dynamics in a freshwater invertebrate, the bivalve Corbicula fluminea . In the laboratory, Corbicula uptake and loss rate constants were determined from an environmentally realistic waterborne exposure to 65 Cu (5.7 μg L - 1 ). That is, we spiked deionized water with Cu that was 99.4% 65 Cu. Net tracer uptake was detectable after 1 day and strongly evident after 4 days. Thus, short-term exposures necessary to determine uptake dynamics are feasible with stable isotopes of Cu. In Corbicula , 65 Cu depuration was biphasic. An unusually low rate constant of loss (0.0038 d - 1 ) characterized the slow component of efflux, explaining why Corbicula strongly accumulates copper in nature. We incorporated our estimates of rate constants for dissolved 65 Cu uptake and physiological efflux into a bioaccumulation model and showed that dietary exposure to Cu is likely an important bioaccumulation pathway for Corbicula .

Environmental Science & Technology

Riverscape heterogeneity shapes population diversity for a migratory fish

Habitat patch dynamics can scale up to influence population demography and diversity with implications for resilience to environmental stochasticity. But how the spatial arrangement and size of habitat patches interact with other components of habitat heterogeneity to shape population diversity at larger spatial scales is not well understood. For riverine fishes, there is increasing evidence that tributary streams provide critical demographic support to main stem rivers. However, the extent to which main stem rivers rely on demographic contributions from tributaries, and the factors underlying this dependence, have not been assessed. Here, we used genetic stock identification to evaluate the effect of tributaries on population diversity of Yellowstone cutthroat trout ( Oncorhynchus virginalis bouvieri ) occupying the main stem Snake River, Wyoming, USA. We found that the main stem relied almost entirely on tributaries for demographic support, but main stem composition varied spatially among river sections. Distance between habitat patches, catchment area, and groundwater availability acted in concert to determine the contribution of specific tributaries to the main stem, but contributions were ultimately modulated by habitat connectivity. We also found evidence for multi-scale spatial structure in tributary contributions, providing insight into untested drivers of main stem river population diversity. Our results demonstrate how spatially discrete and distributed riverscape attributes influence population diversity at broader spatial scales, illustrating how ecosystem resilience emerges from the dynamic, two-way exchange of individuals and energy across habitat networks. Management plans for large rivers that address the ecological contributions of tributaries may be needed to achieve optimal outcomes. Similarly, conservation strategies that exclusively focus on headwater streams may fail to capture the broader habitat requirements necessary to maintain robust cold-water fish populations and associated recreational fisheries, particularly under global environmental change.

Wyoming

Global assessment of aquatic Isoëtes species ecology

Isoëtes are iconic but understudied wetland plants, despite having suffered severe losses globally mainly because of alterations in their habitats. We therefore provide the first global ecological assessment of aquatic Isoëtes to identify their environmental requirements and to evaluate if taxonomically related species differ in their ecology. The assessment resulted in an extensive new database on aquatic Isoëtes , ecological niche analyses, and descriptive species accounts. We compiled a global database that includes all known environmental data collected from 1935 to 2023 regarding aquatic Isoëtes. We then evaluated the environmental drivers of 16 species using 2,179 global records. Additionally, we used hypervolume analysis to quantify the ecological niches of the two species with the greatest number of records, finding significant differences and evidence that Isoëtes echinospora occupies a wider ecological niche than Isoëtes lacustris . Fifty-nine species (30% of the c. 200 Isoëtes species known today) were categorised as aquatic and were mainly reported in the Americas and northern Europe. About 38% of the aquatic species are threatened with extinction or are endemic to a small region, according to the International Union for Conservation of Nature's Red List in 2023. Many species were determined to be sensitive to certain water physical and chemical factors, generally preferring oligotrophic conditions such as low total phosphorus, moderate total nitrogen, moderate to low pH, and low conductivity. This analysis includes ecological data in the assessment of rare/threatened aquatic plants globally. This new database and the ecological analyses completed defined the ecological requirements of several species and identified knowledge gaps, which can aid management actions and future research. This paper highlights ecological significance and environmental sensitivities of aquatic Isoëtes . The current level of knowledge is inadequate for a large proportion of known taxa. We affirm the extreme need to support global, collaborative initiatives on which to build future conservation strategies.

Freshwater Biology

Comparative assessment of a restored and natural wetland using 13C-DNA SIP reveals a higher potential for methane production in the restored wetland

Wetlands are the largest natural source of methane (CH 4 ), a potent greenhouse gas produced by methanogens. Methanogenesis rates are controlled by environmental factors such as redox potential, temperature, and carbon and electron acceptor availability and are presumably dependent on the composition of the active methanogen community. We collected intact soil cores from a restored and natural freshwater depressional wetland on Maryland’s Delmarva Peninsula (USA) to assess the effects of wetland restoration and redox shifts on microbial processes. Intact soil cores were incubated under either saturated (anoxic) or unsaturated (oxic) conditions and amended with 13 C-acetate for quantitative stable isotope probing (qSIP) of the 16S rRNA gene. Restored wetland cores supported a distinct community of methanogens compared to natural cores, and acetoclastic methanogens putatively identified in the genus Methanosarcina were among the most abundant taxa in restored anoxic and oxic cores. The active microbial communities in the restored wetland cores were also distinguished by the unique presence of facultatively anaerobic bacteria belonging to the orders Firmicutes and Bacteroidetes . In natural wetland incubations, methanogen populations were not among the most abundant taxa, and these communities were instead distinguished by the unique presence of aerobic bacteria in the phyla Acidobacteria , Actinobacteria , and class Alphaproteobacteria . Iron-reducing bacteria, in the genus Geobacter , were active across all redox conditions in both the restored and the natural cores, except the natural oxic–anoxic condition. These findings suggest an overall higher potential for methanogenesis in the restored wetland site compared to the natural wetland site, even when there is evidence of Fe reduction.

Maryland

Empirical assessment of fish introductions in a subtropical wetland: An evaluation of contrasting views

We summarized data from eight quantitative fish surveys conducted in southern Florida to evaluate the distribution and relative abundance of introduced fishes across a variety of habitats. These surveys encompassed marsh and canal habitats throughout most of the Everglades region, including the mangrove fringe of Florida Bay. Two studies provided systematically collected density information over a 20-year period, and documented the first local appearance of four introduced fishes based on their repeated absence in prior surveys. Those species displayed a pattern of rapid population growth followed by decline, then persistence at lower densities. Estuarine areas in the southern Everglades, characterized by natural tidal creeks surrounded by mangrove-dominated marshes, and canals held the largest introduced-fish populations. Introduced fishes were also common, at times exceeding 50% of the fish community, in solution holes that serve as dry-season refuges in short-hydroperiod rockland habitats of the eastern Everglades. Wet prairies and alligator ponds distant from canals generally held few individuals of introduced fishes. These patterns suggest that the introduced fishes in southern Florida at present may not be well-adapted to persist in freshwater marshes of the Everglades, possibly because of an interaction of periodic cold-temperature stress and hydrologic fluctuation. Our analyses indicated low densities of these fishes in central or northern Everglades wet-prairie communities, and, in the absence of experimental data, little evidence of biotic effects in this spatially extensive habitat. There is no guarantee that this condition will be maintained, especially under the cumulative effects of future invasions or environmental change.

Biological Invasions

Flowering phenology shifts in response to biodiversity loss

Observational studies and experimental evidence agree that rising global temperatures have altered plant phenology—the timing of life events, such as flowering, germination, and leaf-out. Other large-scale global environmental changes, such as nitrogen deposition and altered precipitation regimes, have also been linked to changes in flowering times. Despite our increased understanding of how abiotic factors influence plant phenology, we know very little about how biotic interactions can affect flowering times, a significant knowledge gap given ongoing human-caused alteration of biodiversity and plant community structure at the global scale. We experimentally manipulated plant diversity in a California serpentine grassland and found that many plant species flowered earlier in response to reductions in diversity, with peak flowering date advancing an average of 0.6 days per species lost. These changes in phenology were mediated by the effects of plant diversity on soil surface temperature, available soil N, and soil moisture. Peak flowering dates were also more dispersed among species in high-diversity plots than expected based on monocultures. Our findings illustrate that shifts in plant species composition and diversity can alter the timing and distribution of flowering events, and that these changes to phenology are similar in magnitude to effects induced by climate change. Declining diversity could thus contribute to or exacerbate phenological changes attributed to rising global temperatures.

Proceedings of the National Academy of Sciences

Prioritization of constituents for national- and regional-scale ambient monitoring of water and sediment in the United States

A total of 2,541 constituents were evaluated and prioritized for national- and regional-scale ambient monitoring of water and sediment in the United States. This prioritization was done by the U.S. Geological Survey (USGS) in preparation for the upcoming third decade (Cycle 3; 2013–23) of the National Water-Quality Assessment (NAWQA) Program. This report provides the methods used to prioritize the constituents and the results of that prioritization. Constituents were prioritized by the NAWQA National Target Analyte Strategy (NTAS) work group on the basis of available information on physical and chemical properties, observed or predicted environmental occurrence and fate, and observed or anticipated adverse effects on human health or aquatic life. Constituents were evaluated within constituent groups that were determined on the basis of physical or chemical properties or on uses or sources. Some constituents were evaluated within more than one constituent group. Although comparable objectives were used in the prioritization of constituents within the different constituent groups, differences in the availability of information accessed for each constituent group led to the development of separate prioritization approaches adapted to each constituent group to make best use of available resources. Constituents were assigned to one of three prioritization tiers: Tier 1, those having the highest priority for inclusion in ambient monitoring of water or sediment on a national or regional scale (including NAWQA Cycle 3 monitoring) on the basis of their likelihood of environmental occurrence in ambient water or sediment, or likelihood of effects on human health or aquatic life; Tier 2, those having intermediate priority for monitoring on the basis of their lower likelihood of environmental occurrence or lower likelihood of effects on human health or aquatic life; and Tier 3, those having low or no priority for monitoring on the basis of evidence of nonoccurrence or lack of effects on human health or aquatic life, or of having insufficient evidence of potential occurrence or effects to justify placement into Tier 2. Of the 1,081 constituents determined to be of highest priority for ambient monitoring (Tier 1), 602 were identified for water and 686 were identified for sediment (note that some constituents were evaluated for both water and sediment). These constituents included various types of organic compounds, trace elements and other inorganic constituents, and radionuclides. Some of these constituents are difficult to analyze, whereas others are mixtures, isomers, congeners, salts, and acids of other constituents; therefore, modifications to the list of high-priority constituents for ambient monitoring could be made on the basis of the availability of suitable methods for preparation, extraction, or analysis. An additional 1,460 constituents were placed into Tiers 2 or 3 for water or sediment, including some constituents that had been placed into Tier 1 for a different matrix; 436 constituents were placed into Tier 2 for water and 246 constituents into Tier 2 for sediment; 979 constituents were placed into Tier 3 for water and 779 constituents into Tier 3 for sediment.

Scientific Investigations Report

Juvenile moose stress and nutrition dynamics related to winter ticks, landscape characteristics, climate-mediated factors and survival

Moose populations in the northeastern United States have declined over the past 15 years, primarily due to the impacts of winter ticks. Research efforts have focused on the effects of winter tick infestation on moose survival and reproduction, but stress and nutritional responses to ticks and other stressors remain understudied. We examined the influence of several environmental factors on moose calf stress hormone metabolite concentrations and nutritional restriction in Vermont, USA. We collected 407 fecal and 461 snow urine samples from 84 radio-collared moose calves in the winters of 2017–2019 (January–April) to measure fecal glucocorticoid metabolites (fGCM) concentrations and urea nitrogen:creatinine (UN:C) ratios. We used generalized mixed-effects models to evaluate the influence of individual condition, winter ticks, habitat, climate and human development on stress and nutrition in calf moose. We then used these physiological data to build generalized linear models to predict calf winter survival. Calf fGCM concentrations increased with nutritional restriction and snow depth during adult winter tick engorgement. Calf UN:C ratios increased in calves with lighter weights and higher tick loads in early winter. Calf UN:C ratios also increased in individuals with home ranges composed of little deciduous forests during adult winter tick engorgement. Our predictive models estimated that winter survival was negatively related to UN:C ratios and positively related to fGCM concentrations, particularly in early winter. By late March, as winter ticks are having their greatest toll and endogenous resources become depleted, we estimated a curvilinear relationship between fGCM concentrations and survival. Our results provide novel evidence linking moose calf stress and nutrition, a problematic parasite and challenging environment and winter survival. Our findings provide a baseline to support the development of non-invasive physiological monitoring for assessing environmental impacts on moose populations.

Vermont

Fishing for conservation of freshwater tropical fish in the Anthropocene

1. Biodiversity and fisheries are two important assets of freshwater ecosystems that are currently at risk from external threats. Establishing an equitable resolution to these threats is a major challenge of the Anthropocene. 2. This is particularly pertinent in developing nations where hotspots for biodiversity converge with rapid, and often environmentally degrading, economic development, and high dependency on aquatic biota and fisheries by local communities. 3. Here, we present global case studies that demonstrate how building upon a shared need for healthy environments and ecological integrity can meet both biodiversity conservation and fishery objectives. 4. These case studies provide evidence that addressing biodiversity conservation needs is possible through partnerships (especially with fishers), shared knowledge, and innovation in fisheries management. 5. In the pursuit of sustainable use of fresh water in development, both conservation and fisheries agendas are better served if efforts focused on synergising fishing activities with local ecosystem functioning and yield long-term livelihood and food security perspectives. 6. A partnership between conservationists (e.g., practitioners, environmental NGOs) and fisheries communities provides a unified voice advocating for biodiversity and social interests in freshwater governance decisions, which has more socio-economic and political capital than when voices are independent or in competition.

Aquatic Conservation: Marine and Freshwater Ecosys

A 2650-year-long record of environmental change from northern Yellowstone National Park based on a comparison of multiple proxy data

Geochemical, stable-isotope, pollen, charcoal, and diatom records were analyzed at high-resolution in cores obtained from Crevice Lake, a varved-sediment lake in northern Yellowstone National Park. The objective was to reconstruct the ecohydrologic, vegetation, and fire history of the watershed for the last 2650 years to better understand past climate variations at the forest-steppe transition. The data suggest a period of limited bottom-water anoxia, relatively wet winters, and cool springs and summers from 2650 to 2100 cal yr BP (700-150 BC). Dry warm conditions occurred between 2100 and 850-800 cal yr BP (150 BC and AD 1100-1150), when the lake was anoxic, winter precipitation was low, and summer stratification was protracted. The data are consistent with overall warmer/drier conditions during the Medieval Climate Anomaly, although they suggest a shift towards wetter winters within that period. The period from 850 to 800 cal yr BP (AD 1100-1150) to 250 cal yr BP (AD 1700) was characterized by greater water-column mixing and cooler spring/summer conditions than before. In addition, fire activity shifted towards infrequent large events and pollen production was low. From 250 to 150 cal yr BP (AD 1700-1800), winter precipitation was moderate compared to previous conditions, and the lake was again stratified, suggesting warm summers. Between 150 and 42 cal yr BP (AD 1800-1908), winter precipitation increased and spring and summer conditions became moderate. Metal pollution, probably from regional mining operations, is evident in the 1870s. Large fires occurred between ca. 1800-1880, but in general the forests were more closed than before. The Crevice Lake record suggests that the last 150 years of Yellowstone's environmental history were characterized by intermediate conditions when compared with the previous 2500 years. ?? 2007 Elsevier Ltd and INQUA.

Quaternary International

Selenium in aquatic biota inhabiting agricultural drains in the Salton Sea Basin, California

Resource managers are concerned that water conservation practices in irrigated farmlands along the southern border of the Salton Sea, Imperial County, California, could increase selenium concentrations in agricultural drainwater and harm the desert pupfish ( Cyprinodon macularius ), a federally protected endangered species. As part of a broader attempt to address this concern, we conducted a 3-year investigation to collect baseline information on selenium concentrations in seven agricultural drains inhabited by pupfish. We collected water, sediment, selected aquatic food-chain taxa (particulate organic detritus, filamentous algae, net plankton, and midge [Chironomidae] larvae), and two poeciliid fishes (western mosquitofish Gambusia affinis and sailfin molly Poecilia latipinna ) for selenium determinations. The two fish species served as ecological surrogates for pupfish, which we were not permitted to sacrifice. Dissolved selenium ranged from 0.70 to 32.8 &mu;g/L, with selenate as the major constituent. Total selenium concentrations in other environmental matrices varied widely among drains, with one drain (Trifolium 18) exhibiting especially high concentrations in detritus, 5.98&ndash;58.0 &mu;g Se/g; midge larvae, 12.7&ndash;50.6 &mu;g Se/g; mosquitofish, 13.2&ndash;20.2 &mu;g Se/g; and mollies, 12.8&ndash;30.4 &mu;g Se/g (all tissue concentrations are based on dry weights). Although toxic thresholds for selenium in fishes from the Salton Sea are still poorly understood, available evidence suggests that ambient concentrations of this element may not be sufficiently elevated to adversely affect reproductive success and survival in selenium-tolerant poeciliids and pupfish.

Environmental Monitoring and Assessment