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On phytoplankton trends

P hytoplankton—unicellular algae in the surface layer of lakes and oceans—fuel the lacustrine and marine food chains and play a key role in regulating atmospheric carbon dioxide concentrations. How will rising carbon dioxide concentrations in the air and surface ocean in turn affect phytoplankton? Answering this question is crucial for projecting future climate change. However, because phytoplankton species populations appear and disappear within weeks, assessing change requires high-resolution monitoring of annual cycles over many years. Such long-term studies at coastal sites ranging from estuaries and harbors to open coastlines and islands are yielding bewildering variability, but also fundamental insights on the driving forces that underlie phytoplankton cycles.

Science

Magnification of secondary production by kelp detritus in coastal marine ecosystems

Kelps are highly productive seaweeds found along most temperate latitude coastlines, but the fate and importance of kelp production to nearshore ecosystems are largely unknown. The trophic role of kelp-derived carbon in a wide range of marine organisms was assessed by a natural experiment. Growth rates of benthic suspension feeders were greatly increased in the presence of organic detritus (particulate and dissolved) originating from large benthic seaweeds (kelps). Stable carbon isotope analysis confirmed that kelp-derived carbon is found throughout the nearshore food web.

Science

A genetic signature of the evolution of loss of flight in the Galapagos cormorant

INTRODUCTION Changes in the size and proportion of limbs and other structures have played a key role in the evolution of species. One common class of limb modification is recurrent wing reduction and loss of flight in birds. Indeed, Darwin used the occurrence of flightless birds as an argument in favor of his theory of natural selection. Loss of flight has evolved repeatedly and is found among 26 families of birds in 17 different orders. Despite the frequency of these modifications, we have a limited understanding of their underpinnings at the genetic and molecular levels. RATIONALE To better understand the evolution of changes in limb size, we studied a classic case of recent loss of flight in the Galapagos cormorant ( Phalacrocorax harrisi ). Cormorants are large water birds that live in coastal areas or near lakes, and P. harrisi is the only flightless cormorant among approximately 40 extant species. The entire population is distributed along the coastlines of Isabela and Fernandina islands in the Galapagos archipelago. P. harrisi has a pair of short wings, which are smaller than those of any other cormorant. The extreme reduction of the wings and pectoral skeleton observed in P. harrisi is an attractive model for studying the evolution of loss of flight because it occurred very recently; phylogenetic evidence suggests that P. harrisi diverged from its flighted relatives within the past 2 million years. We developed a comparative and predictive genomics approach that uses the genome sequences of P. harrisi and its flighted relatives to find candidate genetic variants that likely contributed to the evolution of loss of flight. RESULTS We sequenced and de novo assembled the whole genomes of P. harrisi and three closely related flighted cormorant species. We identified thousands of coding variants exclusive to P. harrisi and classified them according to their probability of altering protein function based on conservation. Variants most likely to alter protein function were significantly enriched in genes mutated in human skeletal ciliopathies, including Ofd1 , Evc , Wdr34 , and Ift122 . We carried out experiments in Caenorhabditis elegans to confirm that a missense variant present in the Galapagos cormorant IFT122 protein is sufficient to affect ciliary function. The primary cilium is essential for Hedgehog (Hh) signaling in vertebrates, and individuals affected by ciliopathies have small limbs and ribcages, mirroring the phenotype of P. harrisi . We also identified a 4–amino acid deletion in the regulatory domain of Cux1 , a highly conserved transcription factor that has been experimentally shown to regulate limb growth in chicken. The four missing amino acids are perfectly conserved in all birds and mammals sequenced to date. We tested the consequences of this deletion in a chondrogenic cell line and showed that it impairs the ability of CUX1 to transcriptionally up-regulate cilia-related genes (some of which contain function-altering variants in P. harrisi ) and to promote chondrogenic differentiation. Finally, we show that positive selection may have played a role in the fixation of the variants associated with loss of flight in P. harrisi . CONCLUSION Our results indicate that the combined effect of variants in genes necessary for the correct transcriptional regulation and function of the primary cilium likely contributed to the evolution of highly reduced wings and other skeletal adaptations associated with loss of flight in P. harrisi . Our approach may be generally useful for identification of variants underlying evolutionary novelty from genomes of closely related species.

Science

Diversity, composition, and geographical distribution of microbial communities in California salt marsh sediments

The Pacific Estuarine Ecosystem Indicators Research Consortium seeks to develop bioindicators of toxicant-induced stress and bioavailability for wetland biota. Within this framework, the effects of environmental and pollutant variables on microbial communities were studied at different spatial scales over a 2-year period. Six salt marshes along the California coastline were characterized using phospholipid fatty acid (PLFA) analysis and terminal restriction fragment length polymorphism (TRFLP) analysis. Additionally, 27 metals, six currently used pesticides, total polychlorinated biphenyls and polycyclic aromatic hydrocarbons, chlordanes, nonachlors, dichlorodiphenyldichloroethane, and dichlorodiphenyldichloroethylene were analyzed. Sampling was performed over large (between salt marshes), medium (stations within a marsh), and small (different channel depths) spatial scales. Regression and ordination analysis suggested that the spatial variation in microbial communities exceeded the variation attributable to pollutants. PLFA analysis and TRFLP canonical correspondence analysis (CCA) explained 74 and 43% of the variation, respectively, and both methods attributed 34% of the variation to tidal cycles, marsh, year, and latitude. After accounting for spatial variation using partial CCA, we found that metals had a greater effect on microbial community composition than organic pollutants had. Organic carbon and nitrogen contents were positively correlated with PLFA biomass, whereas total metal concentrations were positively correlated with biomass and diversity. Higher concentrations of heavy metals were negatively correlated with branched PLFAs and positively correlated with methyl- and cyclo-substituted PLFAs. The strong relationships observed between pollutant concentrations and some of the microbial indicators indicated the potential for using microbial community analyses in assessments of the ecosystem health of salt marshes. Copyright ?? 2006, American Society for Microbiology. All Rights Reserved.

Applied and Environmental Microbiology

A kinematic model for the southern Alaska orocline based on regional fault patterns

Among the most prominent physiographic features of southern Alaska are a series of nested arcuate lineations, including the Denali fault, that parallel the concave-southward southern coastline of the state. These features are generally interpreted as major dextral shear zones that formed in the Late Cretaceous to early Tertiary in response to stresses imposed on the western edge of North America by transcurrent motion and oblique subduction along the North American margin. South-central Alaska consists of a collage of Paleozoic and Mesozoic tectonostratigraphic terranes and overlap assemblages. Following accretion to the continent, these terranes were transported northward along its margin along strike-slip faults such as the ancestral Denali fault that formed by oblique subduction. The terranes would have arrived at about their present position by Eocene time. It is commonly held that southwestern Alaska rotated into its present configuration by the middle Eocene, in response to impingement of northeast Asia against western Alaska, to form the southern Alaska orocline. Subsequent to this rotation during the middle and late Tertiary, southern Alaska terranes were presumably transported through the Alaska orocline by continued dextral movement along faults on the east limb of the orocline, such as the Denali and Tintina. Both initial bending of the crust to form the orocline and subsequent transport of crust through the orocline would result in significant crustal shortening within the bend. A model is suggested herein whereby shortening is accommodated by a system of secondary, northeast-trending thrust faults. The distribution of these faults shows a consistent pattern within the bend: the faults appear to splay off at or near the major dextral shear zones and generally occur west of the orocline’s axis. That these faults occur where deformation would be greatest to crust driven through the bend suggests that the faults are directly related to crustal dynamics within the bend. If this model is correct, one may infer the sense and timing of motion along many faults that otherwise lack or have limited documented histories. The interaction of strike-slip and thrust faults suggested by the model is reflected in the rupture sequence of the November 3, 2002, M7.9 Denali earthquake, which involved both initiation of slip along a previously unknown east-northeast–trending thrust fault and subsequent strike-slip motion along the McKinley strand of the east-west–trending Denali fault. This event is likely due, in part, to stresses imposed by accretion of the Yakutat terrane that is presently working its way into the bend of the orocline and deforming as a result of collision. Faulting along the western margin of the Yakutat terrane resembles that seen in central Alaska within the hinge of the bend. As such, it likely represents a present-day analog for crustal deformation associated with the orocline and may therefore provide clues to earlier stages of crustal deformation in central Alaska.

Alaska

Strand-plain evidence for late Holocene lake-level variations in Lake Michigan

Lake level is a primary control on shoreline behavior in Lake Michigan. The historical record from lake-level gauges is the most accurate source of information on past lake levels, but the short duration of the record does not permit the recognition of long-term patterns of lake-level change (longer than a decade or two). To extend the record of lake-level change, the internal architecture and timing of development of five strand plains of late Holocene beach ridges along the Lake Michigan coastline were studied. Relative lake-level curves for each site were constructed by determining the elevation of foreshore (swash zone) sediments in the beach ridges and by dating basal wetland sediments in the swales between ridges. These curves detect long-term (30+ yr) lake-level variations and differential isostatic adjustments over the past 4700 yr at a greater resolution than achieved by other studies.

Lake Michigan

Upper crustal structure and Neogene tectonic development of the California continental borderland

Multichannel seismic-reflection data, sonobuoy seismic-refraction data, and regional geology are used to define the upper crustal structure of the southern California continental borderland and to delineate the characteristics of the main lithotectonic belts of the region. The Catalina Schist belt is separated on its west side from the gently deformed Nicolas forearc belt by faults that have steep west dips and pronounced normal separations. On its east side the schist belt is bounded by a large detachment fault that dips gently to the east beneath the west edge of the Peninsular Ranges belt at the coastline near Oceanside. The Catalina Schist was uplifted from middle crustal depths and exposed during a major event of extensional tectonism that started in early Miocene time in conjunction with about 10° of clockwise rotation of the western Transverse Ranges belt. Part of the uplift of the Catalina Schist could have occurred on the detachment fault, but it is thought to have mostly occurred on the steep faults that bound the west edge of the schist belt. A large amount of uplift is required, and it probably involved strong footwall flexural deformation in the wake of the translating and rotating western Transverse Ranges and Nicolas forearc belts. Extension, accompanied by probable large amounts of right slip, continued in the borderland region during and after middle Miocene time. The later stage of extension was accompanied by rapid clockwise rotation of the western Transverse Ranges of at least 90°. Most of the borderland, including the belt of schist that was uplifted in early Miocene time, was further deformed into numerous basins and ridges during this stage of oblique extension. The primary driving force for the deformation is thought to have been derived from the rapid northwest motion of the Pacific plate after it had become coupled to the Farallon plate system, which had previously been subducted beneath the borderland.

Geological Society of America Bulletin

Life and death of the resurrection plate: Evidence for its existence and subduction in the northeastern Pacific in Paleocene-Eocene time

Onshore evidence suggests that a plate is missing from published reconstructions of the northeastern Pacific Ooean in Paleocene- Eocene time. The Resurrection plate, named for the Resurrection Peninsula ophiolite near Seward, Alaska, was located east of the Kula plate and north of the Farallon plate. We interpret coeval near-trench magmatism in southern Alaska and the Cascadia margin as evidence for two slab windows associated with trench-ridge-trench (TRT) triple junctions, which formed the western and southern boundaries of the Resurrection plate. In Alaska, the Sanak-Baranof belt of near-trench intrusions records a west-to-east migration, from 61 to 50 Ma, of the northern TRT triple junction along a 2100-km-long section of coastline. In Oregon, Washington, and southern Vancouver Island, voluminous basaltic volcanism of the Siletz River Volcanics, Crescent Formation, and Metchosin Volcanics occurred between ca. 66 and 48 Ma. Lack of a clear age progression of magmatism along the Cascadia margin suggests that this southern triple junction did not migrate significantly. Synchronous near-trench magmatism from southeastern Alaska to Puget Sound at ca. 50 Ma documents the middle Eocene subduction of a spreading center, the crest of which was subparallel to the margin. We interpret this ca. 50 Ma event as recording the subduction-zone consumption of the last of the Resurrection plate. The existence and subsequent subduction of the Resurrection plate explains (1) northward terrane transport along the southeastern Alaska-British Columbia margin between 70 and 50 Ma, synchronous with an eastward-migrating triple junction in southern Alaska; (2) rapid uplift and voluminous magmatism in the Coast Mountains of British Columbia prior to 50 Ma related to subduction of buoyant, young oceanic crust of the Resurrection plate; (3) cessation of Coast Mountains magmatism at ca. 50 Ma due to cessation of subduction, (4) primitive mafic magmatism in the Coast Mountains and Cascade Range just after 50 Ma, related to slab-window magmatism, (5) birth of the Queen Charlotte transform margin at ca. 50 Ma, (6) extensional exhumation of high-grade metamorphic terranes and development of core complexes in British Columbia, Idaho, and Washington, and extensional collapse of the Cordilleran foreland fold-and-thrust belt in Alberta, Montana, and Idaho after 50 Ma related to initiation of the transform margin, (7) enigmatic 53-45 Ma magmatism associated with extension from Montana to the Yukon Territory as related to slab breakup and the formation of a slab window, (8) right-lateral margin-parallel strike-slip faulting in southern and western Alaska during Late Cretaceous and Paleocene time, which cannot be explained by Farallon convergence vectors, and (9) simultaneous changes in Pacific-Farallon and Pacific-Kula plate motions concurrent with demise of the Kula-Resurrection Ridge.

Geological Society of America Bulletin

Porosity development in coastal carbonate aquifers

Geochemical mixing theory suggests that the mixing of seawater and calcite-saturated fresh ground water can result in a solution that is undersaturated with respect to calcite. Previous studies of the mixing of such waters in carbonate rocks along certain coastlines have indicated that this mixing effect may be responsible for significant amounts of calcite dissolution and porosity development. In this study, potential rates of porosity development by calcite dissolution are assessed by combining geochemical mixing theory with the hydrodynamics of fresh-water-salt-water mixing zones in a coupled reaction- transport model. Results from the reaction-path model PHREEQE are used with a variable-density ground-water flow and solute-transport model to simulate an idealized cross section of a coastal carbonate aquifer. Results of the simulations indicate that the dissolution process is sensitive to fresh-water chemistry, ground-water velocities, and sea-level movement. Dissolution potential was evaluated at three field sites, and evidence from those sites is in general agreement with the simulation results. Dissolution rates indicated by the model show that under the proper conditions this dissolution mechanism can produce significant increases in porosity over relatively short spans of geologic time (tens of thousands of years).

Geology

Migration of the Pee Dee River system inferred from ancestral paleochannels underlying the South Carolina Grand Strand and Long Bay inner shelf

Several generations of the ancestral Pee Dee River system have been mapped beneath the South Carolina Grand Strand coastline and adjacent Long Bay inner shelf. Deep boreholes onshore and high-resolution seismic-reflection data offshore allow for reconstruction of these paleochannels, which formed during glacial lowstands, when the Pee Dee River system incised subaerially exposed coastal-plain and continental-shelf strata. Paleochannel groups, representing different generations of the system, decrease in age to the southwest, where the modern Pee Dee River merges with several coastal-plain tributaries at Winyah Bay, the southern terminus of Long Bay. Positions of the successive generational groups record a regional, southwestward migration of the river system that may have initiated during the late Pliocene. The migration was primarily driven by barrier-island deposition, resulting from the interaction of fluvial and shoreline processes during eustatic highstands. Structurally driven, subsurface paleotopography associated with the Mid-Carolina Platform High has also indirectly assisted in forcing this migration. These results provide a better understanding of the evolution of the region and help explain the lack of mobile sediment on the Long Bay inner shelf. Migration of the river system caused a profound change in sediment supply during the late Pleistocene. The abundant fluvial source that once fed sand-rich barrier islands was cut off and replaced with a limited source, supplied by erosion and reworking of former coastal deposits exposed at the shore and on the inner shelf.

South Carolina

Highstand shelf fans: The role of buoyancy reversal in the deposition of a new type of shelf sand body

Although sea-level highstands are typically associated with sediment-starved continental shelves, high sea level does not hinder major river floods. Turbidity currents generated by plunging of sediment-laden rivers at the fluvial-marine interface, known as hyperpycnal flows, allow for cross-shelf transport of suspended sand beyond the coastline. Hyperpycnal flows in southern California have deposited six subaqueous fans on the shelf of the northern Santa Barbara Channel in the Holocene. Using eight cores and nine grab samples, we describe the deposits, age, and stratigraphic architecture of two fans in the Santa Barbara Channel. Fan lobes have up to 3 m of relief and are composed of multiple hyperpycnite beds ∼5 cm to 40 cm thick. Deposit architecture and geometry suggest the hyperpycnal flows became positively buoyant and lifted off the seabed, resulting in well-sorted, structureless, elongate sand lobes. Contrary to conventional sequence stratigraphic models, the presence of these features on the continental shelf suggests that active-margin shelves may locally develop high-quality reservoir sand bodies during sea-level highstands, and that such shelves need not be solely the site of sediment bypass. These deposits may provide a Quaternary analogue to many well-sorted sand bodies in the rock record that are interpreted as turbidites but lack typical Bouma-type features.

Geological Society of America Bulletin

The Kulanaokuaiki-3 tephra, 900 CE: Products of a remarkably energetic pyroclastic eruption at Kīlauea Volcano, Hawaiʻi, USA

Eruptions of Kīlauea Volcano, Hawaiʻi, USA, can be more powerful than previously recognized. The Kulanaokuaiki-3 (K-3) eruption, ca. 900 CE, consisted of two episodes that dispersed lithic wall-rock clasts (Episode 1) and dominantly scoria (Episode 2; VEI-3) across >65 km 2 southeast of the summit. Dense 12 cm blocks of Episode 1 fell 8–10 km from the summit vent, and 2–4 cm lithic lapilli reached the coastline, 17 km from the vent. The Episode 2 deposit is chemically zoned, indicating orderly eruption from a layered magma body analogous to the 1959 Kīlauea Iki lava lake. Olivine-hosted melt inclusions suggest a magma body within 1 km of the surface. Some Episode 1 lithic clasts have magmatic rinds chemically similar to the early Episode 2 scoria, suggesting a genetic link, although each had a distinct eruption mechanism. Southeastward tephra dispersal counter to NE trade winds implies dispersal by jet-stream winds. The dispersal of lithic clasts in Episode 1 cannot be explained by ballistic trajectories or by transport in a buoyant plume. Calculations instead indicate that a jet from a vent with a minimum diameter of 50 m, a velocity of at least 300 m/s, and a duration of ∼60 s could have lifted the lithic clasts into the jet stream. Isopach and isopleth maps for Episode 2 indicate a subplinian column height of 14–18 km and a duration of 2–3 h, assuming constant flux. The Episode 1 conduit probably intersected or otherwise lowered pressure within a compositionally zoned magma body, triggering eruption of the Episode 2 scoria.

Hawaii

Kelp, cobbles, and currents: Biologic reduction of coarse grain entrainment stress

Models quantifying the onset of sediment motion do not typically account for the effect of biotic processes because they are difficult to isolate and quantify in relation to physical processes. Here we investigate an example of the interaction of kelp (Order Laminariales) and coarse sediment transport in the coastal zone, where it is possible to directly quantify and test its effect. Kelp is ubiquitous along rocky coastlines and the impact on ecosystems has been well studied. We develop a physical model to explore the reduction in critical shear stress of large cobbles colonized by Nereocystis luetkeana , or bull kelp. Observations of coarse sediment motion at a site in the Strait of Juan de Fuca (northwest United States–Canada boundary channel) confirm the model prediction and show that kelp reduces the critical stress required for transport of a given grain size by as much as 92%, enabling annual coarse sediment transport rates comparable to those of fluvial systems. We demonstrate that biology is fundamental to the physical processes that shape the coastal zone in this setting.

Geology

Broad-scale climatic influences on rainfall thresholds for debris flows: Adapting thresholds for northern California to southern California

A Landslide Warning System (LWS) operated in the San Francisco Bay region until late 1995. The LWS issued public advisories when rainfall conditions reached or approached critical levels for triggering debris flows ("mudslides"). Interest in an LWS for southern California was revived by the destructive landslides triggered by the storms of January and February 1993 and by the debris-flow problems created by the extensive areas burned in large wildfires the following autumn. Although a number of elements for an LWS already exist in southern California, a critical element must still be developed: the “threshold,” a defined set of values of rainfall intensity and duration that predicts debris-flow initiation within a specified region. Although reliable rainfall/debris-flow thresholds exist for the San Francisco Bay region, climatic dissimilarities between there and southern California produce differ-ences in the thickness, character, and behavior of the hillslope materials that necessi-tate adjustment of the thresholds. Of particular importance are the amount and distribution of precipitation, which, along the California coast, are controlled by ele-vation, distance from the coastline, and storm frequency. Storm frequency, in turn, is strongly correlated with geographic latitude. Although storms are less frequent in southern California, with a consequent decrease in mean annual precipitation, average rainfall amounts for individual storms generally equal those of storms farther north. A procedure is developed for modifying existing rainfall/debris-flow thresholds to account for these changes in precipitation patterns. Then, a set of interim rainfall/ debris-flow thresholds is derived for the greater Los Angeles region. As a demonstra-tion, these interim thresholds are compared with data on rainfall and debris-flow occur-rence during January and February 1993.

California

Migration and stopover strategies of individual Dunlin along the Pacific coast of North America

We radio-marked 18 Dunlin, Calidris alpina (L., 1758), at San Francisco Bay, California, and 11 Dunlin at Grays Harbor, Washington, and relocated 90% of them along the 4200 km long coastline from north of San Francisco Bay to the Yukon-Kuskokwim Delta, Alaska. The Copper River Delta, Alaska, was the single most important stopover site, with 79% of the marked birds detected there. Our second most important site was the Willapa Bay and Grays Harbor complex of wetlands in Washington. The mean length of stay past banding sites ranged from 1.0 to 3.8 days. Controlling for date of departure, birds banded at San Francisco Bay had higher rates of travel to the Copper River Delta than those banded at Grays Harbor. The later a bird left a capture site, the faster it traveled to the Copper River Delta. Length of stay at the Copper River Delta was inversely related to arrival date. We did not find any effect of sex on travel rate or length of stay. Combining the results of this study with our previous work on Western Sandpipers, Calidris mauri (Cabanis, 1875), reveals variation of migration strategies used within and among shorebird species along the eastern Pacific Flyway. ?? 2004 NRC.

Canadian Journal of Zoology

Fluctuating asymmetry and fractal dimension of the sagittal suture as indicators of inbreeding depression in dama and dorcas gazelles

The effects of inbreeding on the developmental instability of skulls of dorcas ( Gazella dorcas ) and dama ( G . dama ) gazelles were investigated. In total, 132 dorcas gazelle skulls and 74 dama gazelle skulls from the Estación Experimental de Zonas Aridas in Almera, Spain, were measured. The fluctuating asymmetry of 9 meristic characters, consisting of the numbers of foramina on the two sides of the skull and mandible, was calculated. Although only the foramen infraorbitalis showed a significant increase in asymmetry with inbreeding in dorcas gazelles, the sum of the foramina in 5 of the skull regions clearly indicates an increase in asymmetry with inbreeding in both dorcas and dama gazelles. The fractal dimension of the sagittal suture was calculated by means of the coastline method. A greater effect of inbreeding on the sagittal suture in dama than in dorcas gazelle was observed, in concordance with the more evident deleterious effects of inbreeding depression in dama than in dorcas gazelles.

Canadian Journal of Zoology

Controls on sediment transport over coral reefs off southwest Puerto Rico: Seasonal patterns and Hurricane Maria

Guánica Bay in southwest Puerto Rico is highly turbid and has some of the highest PCB concentrations in the USA. To investigate how and to what extent the bay waters influence coral reef ecosystem health along the coastline, 6 months of hydrodynamic data were collected at 8 sites on the insular shelf. Bed shear stresses were primarily driven by waves and were weakest at the site closest to La Parguera, located downcoast to the west. Due to the prevailing westward shelf currents, suspended particulate material (SPM) exiting the bay likely settles out at these lower energy sites. We postulate the ecosystem in this area was adversely affected immediately after the hurricane because (1) the source concentration of SPM and PCBs in the bay was greatly increased, and (2) regional waves in the months after the hurricane season were less energetic and, thus, less frequently mobilized and flushed the material.

Puerto Rico

US Costal Research Program: Building a research community to support coastal stakeholders

The U.S. Coastal Research Program (USCRP) was created to develop, coordinate, and enable a National science plan to address growing needs of coastal communities. Researchers from federal agencies, academia, industry, and non-governmental organizations work together to identify priorities that support coastal stakeholders in activities such as emergency response, resource management, planning, and engineering. By fostering existing partnerships and multi-agency collaborations, the USCRP increases the value and impact of these coastal research applications. Through user-driven topical workshops, the USCRP initiates conversation between users and researchers to help ensure that research addresses societal needs along the coastline. By leveraging and expanding federal funding, opportunities are created for coastal science and engineering university programs to advance their research directions, provide graduate student opportunities, and connect their work to National coastal priorities.

Conference Paper