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Louisiana coastal ecosystem

Louisiana's coast and its degradation and restoration are major environmental issues being studied at the National Wetlands Research Center. Coastal ecosystems are vulnerable because of the tremendous amount of human activity that takes place along the coast. Information on ecological processes is essential to guide the development along the coast as well as to protect and restore wildlife habitat. Louisiana has about 40% of coastal wetlands in the lower 48 states; they support fish, waterfowl, and fur-bearing animals as well as unique cultures like that of the Acadians. The fish and wildlife resources of Louisiana's coast produce over $1 billion each year in revenues. But Louisiana has the highest coastal loss rate because of natural and human causes. Over the past three decades, Louisiana has lost as much as 35-40 mi 2 (90-104 km 2 ) of coastal wetlands a year. The National Wetlands Research Center is qualified to assess and monitor this ecosystem because of its proximity to the study area, a staff chosen for their expertise in the system, and a number of established partnerships with others who study the areas. The Center is often the lead group in partnerships with universities, other federal agencies, and private entities who study this ecosystem. Most of the Center's research and technology development performed for coastal wetlands are done at the Lafayette headquarters; some work is performed at the National Wetlands Research Center's project office in Baton Rouge, LA.

Fact Sheet

Chronic wasting disease—Research by the U.S. Geological Survey and partners

Introduction Chronic wasting disease (CWD) is the only transmissible spongiform encephalopathy, a class of invariably fatal neurodegenerative mammalian diseases associated with a misfolded cellular prion protein found in wild free-ranging animals. Because it has a long incubation period, affected animals in Cervidae (the deer family; referred to as “cervids”) may not show signs of disease for several years. While signs are not specific to CWD, affected cervids (deer, elk, moose, and reindeer) show changes in appearance (such as progressive weight loss) and changes in behavior such as stumbling, tremors, and teeth grinding. CWD can be transmitted by direct contact or through a contaminated environment. The causative prion agent is highly resistant to degradation. In recent decades, CWD has transitioned from a novel, obscure prion disease of cervids with limited geographical distribution, to a disease that poses substantial ecological, agricultural, and economic risks across large regions of North America. Since its discovery in free-ranging elk and deer populations in the western United States in the 1980s, CWD has been reported in captive or free-ranging cervid populations in 26 States, 3 Canadian Provinces, the Republic of South Korea, Finland, Sweden, and Norway. In addition, the proportion of CWD-infected animals is increasing in many areas where the disease is already established. In some heavily affected areas, total cervid numbers have decreased over time due to CWD, which suggests that these cervid populations may not be sustainable in the long-term. The U.S. Geological Survey (USGS) conducts wildlife disease surveillance and research to support management of CWD-affected species and their habitats. The scientific information is relevant to governmental agencies that manage wildlife and their habitats including the U.S. Fish and Wildlife Service, the National Park Service, the U.S. Department of Agriculture, and other Federal, State, and Tribal agencies as well as conservation partners (non-governmental organizations, businesses, and private landowners). Each project description in this report (1–30) includes the non-USGS collaborators (Federal, State, Tribal agencies, universities) and a USGS point of contact (principal investigator). If there are USGS publications associated with the project, a publication list is provided at the end of each project description.

Alberta, California, Colorado, Idaho, Iowa, Kansas

Discriminating among Pacific salmon, Rainbow Trout, and Atlantic Salmon species using common genetic screening methods

The five most common species of Pacific salmon, Rainbow Trout (steelhead) Oncorhynchus spp., and Atlantic Salmon Salmo salar intermingle in the North Pacific Ocean and its freshwater tributaries. Efficient morphological methods for distinguishing among these species are sometimes limited by condition of the specimen (degraded or missing morphology), life history stage, or training of the observer. Researchers have successfully applied various genetic methods to distinguish among these species when morphological analyses are not possible, but they cannot easily incorporate these methods into standard fish and wildlife population monitoring analysis workflows. Here we test five 5′–3′ exonuclease (TaqMan) assays developed from mitochondrial genes and provide novel methods that take advantage of TaqMan output to distinguish among these species. We found that combinations of as few as two of the five assays were adequate to distinguish all species. TaqMan chemistry is designed to interrogate a single nucleotide locus. We also explore the basis for the variation in the observed scatter plot distributions (variation in florescent signals) and show that this variation is due to nucleotide diversity in and near the probe site. Because the SNPs underlying the assays developed here are all physically close to one another along the mitochondrial genome, the potential exists to develop a single DNA sequence-based assay to discriminate among salmon species. This single assay can be added to a genotyping-by-sequencing panel to identify and exclude nontarget species from analyses.

Journal of Fish and Wildlife Management

Incorporating temporal variation in seabird telemetry data: time variant kernel density models

A key component of the Mid-Atlantic Baseline Studies project was tracking the individual movements of focal marine bird species (Red-throated Loon [Gavia stellata], Northern Gannet [Morus bassanus], and Surf Scoter [Melanitta perspicillata]) through the use of satellite telemetry. This element of the project was a collaborative effort with the Department of Energy (DOE), Bureau of Ocean Energy Management (BOEM), the U.S. Fish and Wildlife Service (USFWS), and Sea Duck Joint Venture (SDJV), among other organizations. Satellite telemetry is an effective and informative tool for understanding individual animal movement patterns, allowing researchers to mark an individual once, and thereafter follow the movements of the animal in space and time. Aggregating telemetry data from multiple individuals can provide information about the spatial use and temporal movements of populations. Tracking data is three dimensional, with the first two dimensions, X and Y, ordered along the third dimension, time. GIS software has many capabilities to store, analyze and visualize the location information, but little or no support for visualizing the temporal data, and tools for processing temporal data are lacking. We explored several ways of analyzing the movement patterns using the spatiotemporal data provided by satellite tags. Here, we present the results of one promising method: time-variant kernel density analysis (Keating and Cherry, 2009). The goal of this chapter is to demonstrate new methods in spatial analysis to visualize and interpret tracking data for a large number of individual birds across time in the mid-Atlantic study area and beyond. In this chapter, we placed greater emphasis on analytical methods than on the behavior and ecology of the animals tracked. For more detailed examinations of the ecology and wintering habitat use of the focal species in the midAtlantic, see Chapters 20-22.

Report

Standardized methods for Grand Canyon fisheries research 2015

This document presents protocols and guidelines to persons sampling fishes in the Grand Canyon, to help ensure consistency in fish handling, fish tagging, and data collection among different projects and organizations. Most such research and monitoring projects are conducted under the general umbrella of the Glen Canyon Dam Adaptive Management Program and include studies by the U.S. Geological Survey (USGS), U.S. Fish and Wildlife Service (FWS), National Park Service (NPS), the Arizona Game and Fish Department (AGFD), various universities, and private contractors. This document is intended to provide guidance to fieldworkers regarding protocols that may vary from year to year depending on specific projects and objectives. We also provide herein documentation of standard methods used in the Grand Canyon that can be cited in scientific publications, as well as a summary of changes in protocols since the document was first created in 2002.

Grand Canyon

How and why is the timing and occurrence of seasonal migrants in the Gulf of Maine changing due to climate?

Plants and animals undergo certain recurring life-cycle events, such as migrations between summer and winter habitats or the annual blooming of plants. Known as phenology, the timing of these events is very sensitive to changes in climate (and changes in one species’ phenology can impact entire food webs and ecosystems). Shifts in phenology have been described as a “fingerprint” of the temporal and spatial responses of wildlife to climate change impacts. Thus, phenology provides one of the strongest indicators of the adaptive capacity of organisms (or the ability of organisms to cope with future environmental conditions). In this study, researchers are exploring how the timing and occurrence of a number of highly migratory marine animals is changing due to a series of climatic and ecological shifts. First, using existing long-term historical data series, they will determine the direction and magnitude of how migration, abundance, or other phenological factors have changed for marine mammals, sea turtles, and fishes that migrate into the Gulf of Maine on a seasonal basis. Because marine animals are inherently difficult to detect, the team will apply dynamic occupancy models to evaluate seasonal migration patterns and habitat use across multiple habitats in the Gulf of Maine region. The project team will also synthesize regional information on a key, ecologically-important prey fish, sandlance, whose timing and abundance is a strong predictor of the occurrence and behavior of predator species targeted in this study as well as a range of other regional fish and wildlife of conservation and management concern. Results from this component of the project will identify coastal fish and wildlife species that are relatively more or less able to adapt and thus potentially vulnerable to climate change; determine the likely primary drivers of those changes; and identify data gaps and future monitoring needs. Ultimately, this information will be available and useful for regional coastal management and adaptation decisions that will allow managers to effectively plan for the future. In a second component of the project, researchers will focus specifically on changes in migration patterns of the endangered North Atlantic right whale. While shifts in the distribution and time of recurring life events are adaptive responses that may help species cope with climate impacts, they can also lead to changes in how species interact with humans. The North Atlantic right whale is one of the most endangered whale species on the planet. In the North Atlantic Ocean, ship strikes and entanglements with commercial fishing gear represent fatal threats to right whales. Recent reports suggest that North Atlantic right whale migration patterns have changed. Many researchers posit that shifts in migration are responsible for recent increases in the overlap between right whales and human activities, especially fishing. To help understand how changes in right whale movements and behaviors may overlap with ship traffic, and thus put the animals at risk of encountering vessels, we will combine right whale habitat models with ship traffic maps. The end result will be a set of maps identifying risk levels.

Gulf of Maine

A synthesis of patterns of environmental mercury inputs, exposure and effects in New York State

Mercury (Hg) pollution is an environmental problem that adversely affects human and ecosystem health at local, regional, and global scales—including within New York State. More than two-thirds of the Hg currently released to the environment originates, either directly or indirectly, from human activities. Since the early 1800s, global atmospheric Hg concentrations have increased by three- to eight-fold over natural levels. In the U.S., atmospheric emissions and point-source releases to waterways increased following industrialization into the mid-1980s. Since then, water discharges have largely been curtailed. As a result, Hg emissions, atmospheric concentrations, and deposition over the past few decades have declined across the eastern U.S. Despite these decreases, Hg pollution persists. To inform policy efforts and to advance public understanding, the New York State Energy Research and Development Authority (NYSERDA) sponsored a scientific synthesis of information on Hg in New York State. This effort includes 23 papers focused on Hg in atmospheric deposition, water, fish, and wildlife published in Ecotoxicology . New York State experiences Hg contamination largely due to atmospheric deposition. Some landscapes are inherently sensitive to Hg inputs driven by the transport of inorganic Hg to zones of methylation, the conversion of inorganic Hg to methylmercury, and the bioaccumulation and biomagnification along food webs. Mercury concentrations exceed human and ecological risk thresholds in many areas of New York State, particularly the Adirondacks, Catskills, and parts of Long Island. Mercury concentrations in some biota have declined in the Eastern Great Lakes Lowlands and the Northeastern Highlands over the last four decades, concurrent with decreases in water releases and air emissions from regional and U.S. sources. However, widespread changes have not occurred in other ecoregions of New York State. While the timing and magnitude of the response of Hg levels in biota varies, policies expected to further diminish Hg emissions should continue to decrease Hg concentrations in food webs, yielding benefits to the fish, wildlife, and people of New York State. Anticipated improvements in the Hg status of aquatic ecosystems are likely to be greatest for inland surface waters and should be roughly proportional to declines in atmospheric Hg deposition. Efforts that advance recovery from Hg pollution in recent years have yielded significant progress, but Hg remains a pollutant of concern. Indeed, due to this extensive compilation of Hg observations in biota, it appears that the extent and intensity of the contamination on the New York landscape and waterscape is greater than previously recognized. Understanding the extent of Hg contamination and recovery following decreases in atmospheric Hg deposition will require further study, underscoring the need to continue existing monitoring efforts.

New York

International meeting on sarcoptic mange in wildlife, June 2018, Blacksburg, Virginia, USA

Sarcoptic mange is a globally distributed disease caused by the burrowing mite Sarcoptes scabiei , which also causes scabies in humans. A wide and increasing number of wild mammal species are reported to be susceptible to mange; however, the impacts of the disease in wildlife populations, mechanisms involved in its eco-epidemiological dynamics, and risks to public and ecosystem health are still unclear. Major gaps exist concerning S. scabiei host specificity and the mechanisms involved in the different presentations of the disease, which change between individuals and species. Immunological responses to the mite may have a relevant role explaining these different susceptibilities, as these affect the clinical signs, and consequently, the severity of the disease. Recently, some studies have suggested sarcoptic mange as an emerging threat for wildlife, based on several outbreaks with increased severity, geographical expansions, and novel wild hosts affected. Disease ecology experts convened for the “International Meeting on Sarcoptic Mange in Wildlife” on 4–5 June 2018, hosted by the Department of Fish and Wildlife Conservation at Virginia Tech in Blacksburg, Virginia, USA. The meeting had a structure of (i) pre-workshop review; (ii) presentation and discussions; and (iii) identification of priority research questions to understand sarcoptic mange in wildlife. The workgroup concluded that research priorities should be on determining the variation in modes of transmission for S. scabiei in wildlife, factors associated with the variation of disease severity among species, and long-terms effects of the mange in wildlife populations. In this note we summarize the main discussions and research gaps identified by the experts.

Parasites & Vectors

Site-characteristic and hydrologic data for selected wells and springs on Federal land in Clark County, Nevada

Site-characteristic and hydrologic data for selected wells and springs on U.S. Bureau of Land Management, National Park Service, U.S. Fish and Wildlife Service, and U.S. Forest Service land in Clark County, Nevada, were updated in the U.S. Geological Survey’s National Water Information System (NWIS) to facilitate multi-agency research. Data were researched and reviewed, sites were visited, and NWIS data were updated for 231 wells and 198 springs, including 36 wells and 67 springs that were added to NWIS and 44 duplicate sites that were deleted. The site-characteristic and hydrologic data collected, reviewed, edited, and added to NWIS include locations, well water levels, spring discharges, and water chemistry. Site-characteristic and hydrologic data can be accessed from links to the NWIS web interface; data not available through the web interface are presented in appendixes to this report.

Nevada

Using citizen scientists to collect oxygen and hydrogen isotope data in southern Nevada

What is Citizen Science? Citizen science programs provide a means for Federal and non-Federal government agencies to make science more engaging, transparent, and accessible by partnering with the public for the purpose of problem solving, data collection, and monitoring. Public volunteers become directly involved in local research, thereby engaging in scientific projects. The public has already been included in existing citizen science programs that cover a broad range of disciplines, such as ecology, hydrology, and tectonics. Citizen science advances research while simultaneously fostering a sense of involvement and interest from the public. Beginning in 2017, the U.S. Geological Survey (USGS), U.S. Forest Service, Bureau of Land Management, U.S. Fish and Wildlife Service, and National Park Service collaborated with private, non-profit partners to inventory, survey, and rehabilitate springs in Clark County, Nevada. The USGS maintains the National Water Information System (NWIS), a publicly available online database of water-resources data for the Nation, and the agency is interested in using citizen science to add geochemical data from springs in southern Nevada. From 2021 to 2023, the USGS directly worked with citizen science partners, including the Springs Stewardship Institute and the Friends of Nevada Wilderness, to collect stable isotope and tritium samples from southern Nevada springs. The citizen science volunteers were provided the training and supplies for proper sample collection by USGS staff. As the citizen science partners traveled and hiked to the remote spring sites to complete spring surveys and perform restoration activities, they collected stable isotope and tritium samples for the USGS. Samples were shipped to national USGS laboratories for analysis, and the results were uploaded to the NWIS database (U.S. Geological Survey, 2024).

Nevada

Satellite telemetry of marine megavertebrates: The coming of age of an experimental science

Wildlife telemetry research has expanded greatly in the last 2 decades, with the application of satellite tracking and archival logging technologies to study the ecology and conservation of marine mammals, birds, fishes, and turtles. Widespread and expanding use of satellite tracking to study movements and habitats of marine megavertebrates warrants a review of progress to date and a discussion of challenges facing this rapidly evolving research field. To this end, we reviewed the satellite telemetry literature of air-breathing marine taxa (i.e. birds, mammals, turtles) over the 20 yr time period from 1987 to 2006. This review yielded 92 studies with a wide taxonomic representation: 47 seabirds, 23 sea turtles, and 22 marine mammals. Here we critically evaluate these articles to assess progress in satellite tracking of marine megavertebrates in terms of (1) objectives and approach, (2) experimental design, and (3) reporting of results. The overall trends revealed increases in the duration and scope of tracking studies, yet improvements on experimental design and reporting are needed to facilitate comparisons across studies and species. While inherent ecological differences influence the focus and methods of taxon-specific studies, this review highlights specific areas in need of improvement and provides general suggestions for future tracking studies of marine megavertebrates.

Endangered Species Research

Initial biotic survey of Lisbon Bottom, Big Muddy National Fish and Wildlife Refuge

The 2,300-acre Lisbon Bottom Unit, located in central Missouri, became part of the Big Muddy National Fish and Wildlife Refuge (NFWR) after the Great Flood of 1993 devastated the Unit's farmland and network of levees. As a result, interdisciplinary studies were initiated through collaboration among various researches, universities, and State and Federal conservation agencies to investigate the short-term effects of the flood and to expand information about the Missouri River and flood-plain systems. The studies included in these chapters investigate diverse aspects of Lisbon Bottom Unit's physical setting and biota and provide baseline information that managers can use to assess restoration efforts on Lisbon Bottom and other units of the Big Muddy NFWR.

Missouri

Barred owl research needs and prioritization in California

Barred owls (Strix varia) have reached high densities within the range of the northern spotted owl (S. occidentalis caurina) and are rapidly increasing in number within the range of the California spotted owl (S. o. occidentalis). Encroaching populations of barred owls pose a significant competitive threat to the viability of both spotted owl subspecies in California. In response, the Director of the California Department of Fish and Wildlife (CDFW) convened the California Barred Owl Science Team (BOST) to identify and address the threat posed by barred owls to spotted owls in California. BOST is composed of subject matter scientists with a goal to provide objective scientific review and recommendations to CDFW to promote the recovery and conservation of spotted owls in California. In this document, BOST identifies, describes, and prioritizes key research needs for barred owls that have the potential to benefit the conservation of spotted owls in California. Key research needs were identified from multiple in-person and remote meetings of BOST, with considerable input from attending representatives from state and federal natural resource agencies. BOST and liaisons recognize that periodic updates of this document will likely be required as more is learned about the ecology of barred owls in California and research needs and priorities evolve. Research that BOST deemed the most likely to provide a rigorous scientific basis for reducing barred owl populations included experimental removals, along with ecological studies expected to generate information that would provide avenues for the effective management of barred owls. Other high priority research needs include research using biological samples obtained during experimental removals to better understand the ecology of barred owls and the threats they pose to spotted owls and associated wildlife. We conclude the document with a discussion of how projects are related to the State Wildlife Action Plan. In Appendix I, we discuss considerations for maximizing the success of proposed removal experiments and ecological research on barred owls.

Report

Modeling potential impacts of the Garrison Diversion Unit project on Sand Lake and Arrowwood National Wildlife Refuges: a feasibility analysis

The Garrison Diversion Unit (GDU) of the Pick-Sloan Missouri Basin program was authorized in 1965, with the purpose of diverting Missouri River water to the James River for irrigation, municipal and industrial water supply, fish and wildlife habitat, recreation, and flood control. The project was reauthorized in 1986, with the specification that comprehensive studies be conducted to address a variety of issues. One of these ongoing studies addresses potential impacts of GDU construction and operation on lands of the National Wildlife Refuge (NWR) system, including Arrowwood and Sand Lake Refuges (the Refuges) on the James River. A number of concerns at these Refuges have been identified; the primary concerns addressed in this report include increased winter return flows, which would limit control of rough fish; increased turbidity during project construction, which would decrease production of sago pondweed; and increased water level fluctuations in the late spring and early summer, which would destroy the nests of some over-water nesting birds. The facilitated workshop described in this report was conducted February 18-20, 1987, under the joint sponsorship of the U.S. Bureau of Reclamation, the U.S. Fish and Wildlife Service, and the North Dakota Game and Fish Department. The primary objectives of the workshop were to evaluate the feasibility of using simulation modeling techniques to estimate GDU impacts on Arrowwood and Sand Lake Refuges and to suggest enhancements to the James River Refuge monitoring program. The workshop was structured around the formulation of four submodels: a Hydrology and Water Quality submodel to simulate changes in Refuge pool elevations, turnover rates, and water quality parameters (e.g., total dissolved solids, turbidity, dissolved oxygen, nutrients, water temperature, pesticides) due to GDU construction and operation; a Vegetation submodel to simulate concomitant changes in wetland communities (e.g., sago pondweed, wet meadows, deep and shallow marsh); a Fish submodel to estimate changes in abundance or biomass of rough fish (carp, buffalo) and sportfish (northern pike); and a Wildlife submodel to calculate indices of waterfowl abundance or habitat suitability (e.g., for mallards, western grebes, migrating diving ducks, white-faced ibis, egrets, over-water nesters). Submodels considered weekly to monthly changes in pools within a Refuge over a time horizon of 30-50 years. Based on workshop discussions and past experience with impact analysis modeling, a phased modeling approach was recommended for the James River Refuges analysis. The first phase would involve two modeling efforts. The existing Sand Lake hydrology model, and a similar one developed for Arrowwood NWR, would be validated and used to predict changes on pool elevations and winter inflows to each pool for a variety of GDU alternatives. Outputs from simulations would then be evaluated in terms of potential fish and wildlife impacts. For example, the models could generate indices comparing the magnitude and timing of winter inflows for pre- and postproject conditions; fisheries biologists could then use these indices to better quantify their concerns relative to potential changes in the frequency of rough-fish control. The other modeling effort in the first phase would involve developing a sago pondweed growth model to integrate Refuge monitoring data and existing literature and perhaps to address some questions concerning turbidity impacts. A second phase of simulation modeling would be undertaken only if the initial analyses of hydrologic outputs indicated significant potential problems and if monitoring and research projects had clarified some of the biological and physical processes that cannot be modeled reliably at the present time (e.g., resuspension of sediments by carp, immigration and winter mortality of fish, loss of waterfowl nests due to wave action). The second phase would attempt to develop an integrated impact assessment model. In order to address some of the biological and physical processes that presently are not well understood, a number of studies and enhancements to the Refuge monitoring program were suggested. The Hydrology and Water Quality workgroup recommended increasing turbidity and dissolved oxygen sampling, dropping expensive analysis of some trace elements, adding more pesticide analysis (including some biological monitoring), and developing better area-capacity data for the Sand Lake hydrology model. The Vegetation workgroup suggested expanding the number of monitoring stations, monitoring photosynthetically active radiation by depth, and modifying the biomass sampling procedure and schedule. Also suggested were additional analyses of existing Refuge monitoring data and additional field studies concerning sago growth under a variety of environmental conditions and effects of rough fish density on sago. A careful examination of Refuge narrative reports was recommended by the Fish workgroup to characterize conditions that led to various rates of winter-kill. Monitoring enhancement related to a better understanding of fish population dynamics included increasing dissolved oxygen monitoring, continuing present monitoring of fish movement upstream from Jamestown Reservoir into Arrowwood NWR, initiating similar efforts for upstream movement into Sand Lake NWR and downstream movements into both Refuges, and augmenting the present gillnetting program (or replacing it) with sampling for population and age/size structure estimates. The Wildlife workgroup suggested estimating the relative density of mallard nests in over-water and wet meadow nesting areas, estimating the number of western grebe nests lost due to wave action, delineating wet meadows on the Refuge vegetation maps, estimating annual tuber consumption by birds, and monitoring insect/macroinvertebrate abundance. The workgroup also suggested research studies to better understand the relationships between food supplies and the growth and survival of ducklings and young grebes. the workshop discussions also helped identify some suggestions for modifying project features that, if feasible from an engineering and operational standpoint, would reduce impacts on Refuge lands. These suggestions included: designing drains with control structures or small "reregulation" reservoirs to hold winter return flows that might adversely affect rough fish control, spreading construction activities over a number of years to reduce potential impacts of turbidity on sago pondweed in any single year, scheduling construction to occur after the spring sprouting and elongation growth stages to reduce impacts on sago pondweed, and installing "quick acting" control structures at Arrowwood NWR to reduce pool level fluctuations that might destroy nests of some over-water nesting waterfowl.

Report

U.S. Department of the Interior Climate Science Centers and U.S. Geological Survey National Climate Change and Wildlife Science Center—Annual report for 2015

2015 was another great year for the Department of the Interior (DOI) Climate Science Centers (CSCs) and U.S. Geological Survey (USGS) National Climate Change and Wildlife Science Center (NCCWSC) network. The DOI CSCs and USGS NCCWSC continued their mission of providing the science, data, and tools that are needed for on-the-ground decision making by natural and cultural resource managers to address the effects of climate change on fish, wildlife, ecosystems, and communities. Our many accomplishments in 2015 included initiating a national effort to understand the influence of drought on wildlife and ecosystems; providing numerous opportunities for students and early career researchers to expand their networks and learn more about climate change effects; and working with tribes and indigenous communities to expand their knowledge of and preparation for the impacts of climate change on important resources and traditional ways of living. Here we illustrate some of these 2015 activities from across the CSCs and NCCWSC.

Open-File Report

Impact of wildfire on levels of mercury in forested watershed systems: Voyageurs National Park, Minnesota

Atmospheric deposition of mercury to remote lakes in mid-continental and eastern North America has increased approximately threefold since the mid-1800s (Swain and others, 1992; Fitzgerald and others, 1998; Engstrom and others, 2007). As a result, concerns for human and wildlife health related to mercury contamination have become widespread. Despite an apparent recent decline in atmospheric deposition of mercury in many areas of the Upper Midwest (Engstrom and Swain, 1997; Engstrom and others, 2007), lakes in which fish contain levels of mercury deemed unacceptable for human consumption and possibly unacceptable for fish-consuming wildlife are being detected with increasing frequency. In northern Minnesota, Voyageurs National Park (VNP) (fig. 1) protects a series of southern boreal lakes and wetlands situated on bedrock of the Precambrian Canadian Shield. Mercury contamination has become a significant resource issue within VNP as high concentrations of mercury in loons, bald eagle eaglets, grebes, northern pike, and other species of wildlife and fish have been found. The two most mercury-contaminated lakes in Minnesota, measured as methylmercury in northern pike ( Esox lucius ), are in VNP. Recent multidisciplinary U.S. Geological Survey (USGS) research demonstrated that the bulk of the mercury in lake waters, soils, and fish in VNP results from atmospheric deposition (Wiener and others, 2006). The study by Wiener and others (2006) showed that the spatial distribution of mercury in watershed soils, lake waters, and age-1 yellow perch ( Perca flavescens ) within the Park was highly variable. The majority of factors correlated for this earlier study suggested that mercury concentrations in lake waters and age-1 yellow perch reflected the influence of ecosystem processes that affected within-lake microbial production and abundance of methylmercury (Wiener and others, 2006), while the distribution of mercury in watershed soils seemed to be partially dependent on forest disturbance, especially the historic forest fire pattern (Woodruff and Cannon, 2002). Forest fire has an essential role in the forest ecosystems of VNP (Heinselman, 1996). Because resource and land managers need to integrate both natural wildfire and prescribed fire in management plans, the potential influence of fire on an element as sensitive to the environment as mercury becomes a critical part of their decisionmaking. A number of recent studies have shown that while fire does have a significant impact on mercury at the landscape level, the observed effects of fire on aquatic environments are highly variable and unpredictable (Caldwell and others, 2000; Garcia and Carrigan, 2000; Kelly and others, 2006; Nelson and others, 2007). Caldwell and others (2000) described an increase in methylmercury in reservoir sediments resulting from mobilization and transport of charred vegetative matter following a fire in New Mexico. Krabbenhoft and Fink (2000) attributed increases in total mercury concentrations in young-of-the-year fish in the Florida Everglades to release of mercury resulting from peat oxidation following fires. A fivefold increase in whole-body mercury accumulation by rainbow trout ( Oncorhynchus mykiss ) following a fire in Alberta, Canada, apparently resulted from increased nutrient concentrations that enhanced productivity and restructured the food web of a lake within the fire’s burn footprint (Kelly and others, 2006). For this study, we determined the short-term effects of forest fire on mercury concentrations in terrestrial and aquatic environments in VNP by comparing and contrasting mercury concentrations in forest soils, lake waters, and age-1 yellow perch for a burned watershed and an adjacent lake, with similar samples from watersheds and lakes with no fire activity (control watersheds and lakes). The concentration of total mercury in whole, 1-year-old yellow perch serves as a good biological indicator for monitoring trends in methylmercury concentrations in food webs of lakes in North America (Wiener and others, 2007). With a limited gape, age-1 yellow perch that hatched the previous year and resided in a lake for 1 year feed largely on zooplankton and small benthic invertebrates. Thus, age-1 yellow perch provide a baseline for methylmercury concentrations for individual lakes that can be compared across spatial areas. The nine appendixes that accompany this report contain the complete datasets for soils, lake waters, and age-1 yellow perch collected for this study. This report uses data from these three media to provide a framework for evaluating short-term effects of fire on mercury in forested soils and possible effects of the mobilization of mercury from soils on lake water quality and aquatic health.

Minnesota

Mercury monitoring in fish using a non-lethal tissue biopsy method

The occurrence of mercury in fish is well-known and often occurs at levels that warrant restricted consumption by sensitive human populations. Because of this, local wildlife and health agencies have developed monitoring programs to identify the magnitude of fish contamination and changes through time. Monitoring mercury levels in fish typically requires killing fish for removal of a fillet. Recently, researchers have proposed the use of a non-lethal tissue biopsy plug method as a surrogate for analysis of the entire fillet. A non-lethal method is particularly desirable for sampling rare or endangered fish or highly valued fisheries. The Missouri Department of Conservation manages several fisheries where the public is sensitive to excessive fish removal, yet there is a desire for mercury information. One such example is the trophy smallmouth bass (Micropterus dolomieu) fishery in the Ozark’s Eleven Point River. Plug removal is not expected to affect fish survival in the shortterm. However, limited information is available on survival of fish for weeks or months after plug removal.

Missouri

Efficiency of sampling sunfishes using snorkeling in clear, warmwater streams of the south-central United States

The continued evaluation of fish-sampling gears and methods is essential to identify their applicability across environmental conditions and among species. Although limited by visibility, snorkeling has potential advantages relative to other fish-sampling gears in wadeable streams (e.g., minimally intrusive, cost effective, and appropriate in deeper areas). Clear water is common to warm-water streams; however, the use of snorkeling for monitoring stream-fish populations has largely focused on cold-water systems. To assess relative snorkeling efficiency in warm-water streams, we compared standardized single-pass snorkel counts to tow-barge electrofishing abundance estimates for six sunfishes (Centrarchidae) in the Ozark Highlands ecoregion of northwest Oklahoma and southwest Missouri under relatively similar environmental conditions (i.e., clear water, cobble substrates, low-flow conditions). Snorkeling efficiency was variable among sunfishes and consistently low for species with cryptic traits and habitat use. We also did not detect cryptic sunfishes (i.e., a single individual was not encountered) using snorkeling at multiple stream reaches where estimated abundance was > 50 within a 0.5- to 1.0-km stream reach. Our findings indicate that snorkeling has applications for monitoring sunfish populations and assemblages when using an abundance estimator or accounting for imperfect detection; however, it is inappropriate for estimating population size of cryptic sunfishes. We encourage continued research into the applicability of snorkeling to estimate warm-water stream fish abundance.

Missouri, Oklahoma