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At least 289 records · Page 16Linked to original sources

Drowning of the - 150 m reef off Hawaii: A casualty of global meltwater pulse 1A?

We present evidence that the drowning of the - 150 m coral reef around Hawaii was caused by rapid sea-level rise associated with meltwater pulse 1A (MWP-1A) during the last deglaciation. New U/Th and 14C accelerator mass spectrometry dates, combined with reinterpretation of existing radiometric dates, constrain the age of the coral reef to 15.2-14.7 ka (U/Th age), indicating that reef growth persisted for 4.3 k.y. following the end of the Last Glacial Maximum at 19 ka. The drowning age of the reef is roughly synchronous with the onset of MWP-1A between 14.7 and 14.2 ka. Dates from coralline algal material range from 14 to 10 cal ka (calibrated radiocarbon age), 1-4 k.y. younger than the coral ages. A paleoenvironmental reconstruction incorporating all available radiometric dates, high-resolution bathymetry, dive observations, and coralgal paleobathymetry data indicates a dramatic rise in sea level around Hawaii ca. 14.7 ka. Paleowater depths over the reef crest increased rapidly above a critical depth (30-40 m), drowning the shallow reef-building Porites corals and causing a shift to deepwater coralline algal growth, preserved as a crust on the drowned reef crest. ?? 2004 Geological Society of America.

Geology

Widespread retreat of coastal habitat is likely at warming levels above 1.5 °C

Several coastal ecosystems—most notably mangroves and tidal marshes—exhibit biogenic feedbacks that are facilitating adjustment to relative sea-level rise (RSLR), including the sequestration of carbon and the trapping of mineral sediment 1 . The stability of reef-top habitats under RSLR is similarly linked to reef-derived sediment accumulation and the vertical accretion of protective coral reefs 2 . The persistence of these ecosystems under high rates of RSLR is contested 3 . Here we show that the probability of vertical adjustment to RSLR inferred from palaeo-stratigraphic observations aligns with contemporary in situ survey measurements. A deficit between tidal marsh and mangrove adjustment and RSLR is likely at 4 mm yr −1 and highly likely at 7 mm yr −1 of RSLR. As rates of RSLR exceed 7 mm yr −1 , the probability that reef islands destabilize through increased shoreline erosion and wave over-topping increases. Increased global warming from 1.5 °C to 2.0 °C would double the area of mapped tidal marsh exposed to 4 mm yr −1 of RSLR by between 2080 and 2100. With 3 °C of warming, nearly all the world’s mangrove forests and coral reef islands and almost 40% of mapped tidal marshes are estimated to be exposed to RSLR of at least 7 mm yr −1 . Meeting the Paris agreement targets would minimize disruption to coastal ecosystems.

Nature

Is it time to give up?

The world's coral reefs show rapid decline as a result of environmental change. Coral reef communities and organisms are stressed, potentially mortally, by (1) rising temperature, (2) rising atmospheric/surface ocean CO2 levels, (3) rising human populations, and (4) local aspects of climate change other than temperature. Further increase in all of these stressors is certain; the future rates and magnitudes of items 1-3 can be estimated with confidence to be substantially greater than changes in the recent past.

Conference Paper

Microbial aggregates within tissues infect a diversity of corals throughout the Indo-Pacific

Coral reefs are highly diverse ecosystems where symbioses play a pivotal role. Corals contain cell-associated microbial aggregates (CAMA), yet little is known about how widespread they are among coral species or the nature of the symbiotic relationship. Using histology, we found CAMA within 24 species of corals from 6 genera from Hawaii, American Samoa, Palmyra, Johnston Atoll, Guam, and Australia. Prevalence (%) of infection varied among coral genera: Acropora, Porites, and Pocillopora were commonly infected whereas Montipora were not. Acropora from the Western Pacific were significantly more likely to be infected with CAMA than those from the Central Pacific, whereas the reverse was true for Porites. Compared with apparently healthy colonies, tissues from diseased colonies were significantly more likely to have both surface and basal body walls infected. The close association of CAMA with host cells in numerous species of apparently healthy corals and lack of associated cell pathology reveals an intimate agent-host association. Furthermore, CAMA are Gram negative and in some corals may be related to chlamydia or rickettsia. We propose that CAMA in adult corals are facultative secondary symbionts that could play an important ecological role in some dominant coral genera in the Indo-Pacific. CAMA are important in the life histories of other animals, and more work is needed to understand their role in the distribution, evolution, physiology, and immunology of reef corals.

Marine Ecology Progress Series

Spurs and grooves revisited: construction versus erosion, Looe Key Reef, Florida

Six of 12 core holes drilled at Looe Key Reef (24°37'18"N. 81°24'24"W) by a diver-operated coring device penetrated a spur and groove system. Drilling indicated that: (II the spurs and grooves formed over at least 5 m of carbonate reef sand: (2) the underlying Pleistocene surface is essentially flat and therefore could not control or initiate spacing of spurs or grooves; (3) only the thin seaward ends of spurs are rooted on underlying bedrock: and (4) the interior of the Millepora-encrusted spurs is composed primarily of Acropora palmata. a species no longer abundant on this reef. From the drilling of Looe Key Reef and from other observations along the reef tract, we propose that most shallow spurs and grooves in active coral reef areas of the Caribbean are constructional in origin and not initiated or controlled by bedrock topography. Spurs and grooves in non-coral reef areas adjacent to shorelines, however. are clearly of erosional origin and have a close spacing distinctly different from spurs and grooves known to be of constructional origin. These observations indicate that spurs and grooves in deeper (> 15 m) fore-reef areas off Florida, which have the same geometry as the shoreline features. are erosional in origin and therefore formed on a shoreline during a lower stand of sea level.

Florida

Demographic patterns in the peacock grouper ( Cephalopholis argus ), an introduced Hawaiian reef fish

This study took advantage of a unique opportunity to collect large sample sizes of a coral reef fish species across a range of physical and biological features of the Hawaiian Archipelago to investigate variability in the demography of an invasive predatory coral reef fish, Cephalopholis argus (Family: Epinephelidae). Age-based demographic analyses were conducted at 10 locations in the main Hawaiian Islands and estimates of weight-at-length, size-at-age, and longevity were compared among locations. Each metric differed among locations, although patterns were not consistent across metrics. Length-weight relationships for C. argus differed among locations and individuals weighed less at a given length at Hilo, the southernmost location studied. Longevity differed among and within islands and was greater at locations on Maui and Hawaii compared to the more northern locations on Oahu and Kauai. Within-island growth patterns differed at Kauai, Oahu, and Hawaii. This work provides a case study of fundamental life history information from distant and/or spatially limited locations that are critical for developing robust fishery models. The differences observed both among and within islands indicate that variability may be driven by cross-scale mechanisms that need to be considered in fisheries stock assessments and ecosystem-based management.

Hawai'i

Historic impact of watershed change and sedimentation to reefs along west-central Guam

Using coral growth parameters (extension, density, calcification rates, and luminescence) and geochemical measurements (barium to calcium rations; Ba/Ca) from coral cores collected in west-central Guam, we provide a historic perspective on sediment input to coral reefs adjacent to the Piti-Asan watershed. The months of August through December are dominated by increased coral Ba/Ca values, corresponding to the rainy season. With river water enriched in barium related to nearshore seawater, coral Ba/Ca ratios are presented as a proxy for input of fine-grained terrigenous sediment to the nearshore environment. The century-long Ba/Ca coral record indicates that the Asan fore reef is within the zone of impact from discharged sediments transported from the Piti-Asan watershed and has experienced increased terrestrial sedimentation since the 1940s. This abrupt shift in sedimentation occurred at the same time as both the sudden denudation of the landscape by military ordinance and the immediate subsequent development of the Asan area through the end of the war, from 1944 through 1945. In response to rapid input of sediment, as determined from coral Ba/Ca values, coral growth rates were reduced for almost two decades, while calcification rates recovered much more quickly. Furthermore, coral luminescence is decoupled from the Ba/Ca record, which is consistent with degradation of soil organic matter through disturbance by forest fires, suggesting a potential index of fire history and degradation of soil organic matter. These patterns were not seen in the cores from nearby reefs associated with watersheds that have not undergone the same degree of landscape denudation. Taken together, these records provide a valuable tool for understanding the compounding effects of land-use change on coral reef health.

Agat Bay;Apra Harbor;Asan Bay

Quantifying resilience

The biosphere is under unprecedented pressure, reflected in rapid changes in our global ecological, social, technological and economic systems. In many cases, ecological and social systems can adapt to these changes over time, but when a critical threshold is surpassed, a system under stress can undergo catastrophic change and reorganize into a different state. The concept of resilience, introduced more than 40 years ago in the ecological sciences, captures the behaviour of systems that can occur in alternative states. The original definition of resilience forwarded by Holling ( 1973 ) is still the most useful. It defines resilience as the amount of disturbance that a system can withstand before it shifts into an alternative stable state. The idea of alternative stable states has clear and profound implications for ecological management. Coral reefs, for example, are high-diversity systems that provide key ecosystem services such as fisheries and coastal protection. Human impacts are causing significant, ongoing reef degradation, and many reefs have shifted from coral- to algal-dominated states in response to anthropogenic pressures such as elevated water temperatures and overfishing. Understanding and differentiating between the factors that help maintain reefs in coral-dominated states vs. those that facilitate a shift to an undesired algal-dominated state is a critical step towards sound management and conservation of these, and other, important social–ecological systems. Resilience has gained popularity among both academicians and laypeople, as a term meant to describe a systems’ ability to withstand disturbance. Resilience has become a buzzword in the last decade, as shown by its increasing appearance in calls for research proposals and scientific citation data bases. The term resilience has in many cases lost the clarity of the original definition and in fact is frequently used in a manner in direct opposition to the original definition. Many current uses of the concept are loose and incorrect. The term is becoming increasingly used in a normative sense (Brand & Jax 2007 ), as if resilience were a desirable quality of systems. However, even systems in highly undesirable states, such as macro-algae dominated reefs, or city cores in poverty traps, may be highly resilient, which is to say they withstand attempts to transform them into different (desirable) states. Operationalizing the concept of resilience for application and management has been difficult. Misuse of the term can have significant negative impacts, because resilience is being used to help guide responses to natural disasters and to assess the sustainability of ecosystems and urban systems and has been driving international research priorities. Resilience has been argued to be a basic emergent property of systems, a process or a rate. We focus on the original concept as described by Holling, which is that of an emergent system property; when a system is in a desirable state and managers wish to enhance resilience, or when the system is in an undesirable state and managers wish to erode resilience and foster a transformation to an alternative state. Fostering or eroding resilience is a process. When a system is perturbed but resilience is not exceeded, then the recovery can be measured as a rate. Several frameworks to operationalize resilience have been proposed. A decade ago, a special feature focused on quantifying resilience was published in the journal Ecosystems (Carpenter, Westley & Turner 2005 ). The approach there was towards identifying surrogates of resilience, but few of the papers proposed quantifiable metrics. Consequently, many ecological resilience frameworks remain vague and difficult to quantify, a problem that this special feature aims to address. However, considerable progress has been made during the last decade (e.g. Pope, Allen & Angeler 2014 ). Although some argue that resilience is best kept as an unquantifiable, vague concept (Quinlan et al . 2016 ), to be useful for managers, there must be concrete guidance regarding how and what to manage and how to measure success (Garmestani, Allen & Benson 2013 ; Spears et al . 2015 ). Ideas such as ‘resilience thinking’ have utility in helping stakeholders conceptualize their systems, but provide little guidance on how to make resilience useful for ecosystem management, other than suggesting an ambiguous, Goldilocks approach of being just right (e.g. diverse, but not too diverse; connected, but not too connected). Here, we clarify some prominent resilience terms and concepts, introduce and synthesize the papers in this special feature on quantifying resilience and identify core unanswered questions related to resilience.

Journal of Applied Ecology

Collection methods and descriptions of coral cores extracted from massive corals in Dry Tortugas National Park, Florida, U.S.A.

Cores from living coral colonies were collected from Dry Tortugas National Park, Florida, U.S.A., to obtain skeletal records of past coral growth and allow geochemical reconstruction of environmental variables during the corals’ centuries-long lifespans. The samples were collected as part of the U.S. Geological Survey Coral Reef Ecosystems Studies project ( http:/coastal.er.usgs.gov/crest ) that provides science to assist resource managers tasked with the stewardship of coral reef resources. Three colonies each of the coral species Orbicella faveolata and Siderastrea siderea were collected in May 2012 using the methods described herein and as approved under National Park Service scientific collecting permit number DRTO-2012-SCI-0001 and are cataloged under accession number DRTO-353. These coral samples can be used to retroactively construct environmental parameters, including sea-surface temperature, by measuring the elemental composition of the coral skeleton. The cores described here, and others (see http://olga.er.usgs.gov/coreviewer/ ), can be requested, on loan, for scientific study. Photographic images for each coral in its ocean environment, the coral cores as curated and slabbed, and the X-rays of the slabs can be found in an associated U.S. Geological Survey Data Release.

Florida

Fijian sea krait behavior relates to fine-scale environmental heterogeneity in old-growth coastal forest: The importance of integrated land–sea management for protecting amphibious animals

The importance of terrestrial coastal ecosystems for maintaining healthy coral reef ecosystems remains understudied. Sea kraits are amphibious snakes that require healthy coral reefs for foraging, but little is known about their requirements of terrestrial habitats, where they slough their skin, digest prey, and breed. Using concurrent microclimate measurements and behavior surveys, we show that a small, topographically flat atoll in Fiji with coastal forest provides many microhabitats that relate to the behaviors of Yellow Lipped Sea Kraits, Laticauda colubrina . Microclimates were significantly related to canopy cover, leaf litter depth, and distance from the high-water mark (HWM). Sea kraits were almost exclusively observed in coastal forest within 30 m of the HWM. Sloughing of skins only occurred within crevices of mature or dying trees. Resting L. colubrina were significantly more likely to occur at locations with higher mean diurnal temperatures, lower leaf litter depths, and shorter distances from the HWM. On Leleuvia, behavior of L. colubrina therefore relates to environmental heterogeneity created by old-growth coastal forests, particularly canopy cover and crevices in mature and dead tree trunks. The importance of healthy coastal habitats, both terrestrial and marine, for L. colubrina suggests it could be a good flagship species for advocating integrated land-sea management. Furthermore, our study highlights the importance of coastal forests and topographically flat atolls for biodiversity conservation. Effective conservation management of amphibious species that utilize land- and seascapes is therefore likely to require a holistic approach that incorporates connectivity among ecosystems and environmental heterogeneity at all relevant scales.

Ecology and Evolution

Regional estimates of reef carbonate dynamics and productivity Using Landsat 7 ETM+, and potential impacts from ocean acidification

Using imagery at 30 m spatial resolution from the most recent Landsat satellite, the Landsat 7 Enhanced Thematic Mapper Plus (ETM+), we scale up reef metabolic productivity and calcification from local habitat-scale (10 -1 to 10 0 km 2 ) measurements to regional scales (10 3 to 10 4 km 2 ). Distribution and spatial extent of the North Florida Reef Tract (NFRT) habitats come from supervised classification of the Landsat imagery within independent Landsat-derived Millennium Coral Reef Map geomorphologic classes. This system minimizes the depth range and variability of benthic habitat characteristics found in the area of supervised classification and limits misclassification. Classification of Landsat imagery into 5 biotopes (sand, dense live cover, sparse live cover, seagrass, and sparse seagrass) by geomorphologic class is >73% accurate at regional scales. Based on recently published habitat-scale in situ metabolic measurements, gross production (P = 3.01 ?? 10 9 kg C yr -1 ), excess production (E = -5.70 ?? 10 8 kg C yr -1 ), and calcification (G = -1.68 ?? 10 6 kg CaCO 3 yr -1 ) are estimated over 2711 km 2 of the NFRT. Simple models suggest sensitivity of these values to ocean acidification, which will increase local dissolution of carbonate sediments. Similar approaches could be applied over large areas with poorly constrained bathymetry or water column properties and minimal metabolic sampling. This tool has potential applications for modeling and monitoring large-scale environmental impacts on reef productivity, such as the influence of ocean acidification on coral reef environments. ?? Inter-Research 2009.

Marine Ecology Progress Series

Coral restoration for coastal resilience: Integrating ecology, hydrodynamics, and engineering at multiple scales

The loss of functional and accreting coral reefs reduces coastal protection and resilience for tropical coastlines. Coral restoration has potential for recovering healthy reefs that can mitigate risks from coastal hazards and increase sustainability. However, scaling up restoration to the large extent needed for coastal protection requires integrated application of principles from coastal engineering, hydrodynamics, and ecology across multiple spatial scales, as well as filling missing knowledge gaps across disciplines. This synthesis aims to identify how scientific understanding of multidisciplinary processes at interconnected scales can advance coral reef restoration. The work is placed within the context of a decision support framework to evaluate the design and effectiveness of coral restoration for coastal resilience. Successfully linking multidisciplinary science with restoration practice will ensure that future large-scale coral reef restorations maximize protection for at-risk coastal communities.

Ecosphere

Long- versus short-term changes in seafloor elevation and volume of the Upper Florida Keys Reef Tract: 1935–2002 and 2002–2016

Coral reefs provide immense ecosystem and economic value, supporting biodiversity, fisheries, tourism, and coastal protection worth billions annually. However, widespread degradation from thermal stress, storms, disease, and human impacts has caused significant coral cover and reef structure loss, increasing coastal vulnerability and economic risks. While coral loss is well-documented, degradation of underlying reef infrastructure and surrounding seafloor changes remain poorly understood. This study addresses this knowledge gap by quantifying seafloor elevation and volume changes across 234.2 km 2 of the Upper Florida Keys (UFK) reef tract using historical bathymetric and modern lidar (light detection and ranging) data collected from two periods with distinctly different disturbance regimes: 1935–2002 (frequent storms and major coral loss) and 2002–2016 (few storms and persistently low coral cover). Analysis of over 25,000 data points revealed substantial elevation and volume loss during 1935–2002 (−0.1 ± 0.8 m; 13.6 × 10 6 m 3 net loss), shifting to minimal gains by 2002–2016 (0.0 ± 0.3 m; 1.6 × 10 6 m 3 net gain). Despite this shift, benthic cover data showed continued declines in stony coral, with increases in macroalgae and octocorals, indicating that limited reef accretion persists even with reduced storm activity. Spatial analyses highlighted variable accretion and erosion patterns across habitats and subregions, underscoring the limitations of localized measurements for ecosystem-wide assessments. Our findings demonstrate the value of integrating historical and modern datasets for regional reef monitoring, establishing baselines for restoration planning, and emphasizing the need for continued high-resolution monitoring to guide adaptive management amid ongoing environmental change.

Florida

Reconnaissance geology of the Wadi Amq Quadrangle, sheet 18/41 C, Kingdom of Saudi Arabia

The Wadi Amq quadrangle (sheet 18/41 C) lies between lat 18?00' and 18?30' N. and long 41?00' and 41?30' E. and encompasses an area of 2,937 km2, of which only about 150 km2 is land; the remainder is covered by the Red Sea. The only geologic formations exposed in the quadrangle are Quaternary volcanic rocks and surficial deposits. Most of the volcanic rocks are basalt that was extruded during a continuation of the opening of the Red Sea rift. The Quaternary surficial deposits include those of shallow bank and coral reefs, carbonate sand, sabkhahs, pediment and plains, and alluvial sand and gravel. The coral reefs could possibly provide raw material for a cement industry if required, and the sabkhahs might yield useful salts; otherwise the quadrangle has no economic mineral potential.

Open-File Report

Calcification rates of the massive coral Siderastrea siderea and crustose coralline algae along the Florida Keys (USA) outer-reef tract

Coral reefs are degrading on a global scale, and rates of reef-organism calcification are predicted to decline due to ocean warming and acidification. Systematic measurements of calcification over space and time are necessary to detect change resulting from environmental stressors. We established a network of calcification monitoring stations at four managed reefs along the outer Florida Keys Reef Tract (FKRT) from Miami to the Dry Tortugas. Eighty colonies (in two sequential sets of 40) of the reef-building coral, Siderastrea siderea , were transplanted to fixed apparatus that allowed repetitive detachment for buoyant weighing every 6 months. Algal-recruitment tiles were also deployed during each weighing interval to measure net calcification of the crustose coralline algal (CCA) community. Coral-calcification rates were an order of magnitude greater than those of CCA. Rates of coral calcification were seasonal (summer calcification was 53% greater than winter), and corals in the Dry Tortugas calcified 48% faster than those at the other three sites. Linear extension rates were also highest in the Dry Tortugas, whereas percent area of the coral skeletons excavated by bioeroding fauna was lowest. The spatial patterns in net coral calcification revealed here correlate well with Holocene reef thickness along the FKRT and, in part, support the “inimical waters hypothesis” proposed by Ginsburg, Hudson, and Shinn almost 50 yrs ago to explain reef development in this region. Due to the homogeneity in coral-calcification rates among the three main Keys sites, we recommend refinement of this hypothesis and suggest that water-quality variables (e.g., carbonate mineral saturation state, dissolved and particulate organic matter, light attenuation) be monitored alongside calcification in future studies. Our results demonstrate that our calcification monitoring network presents a feasible and worthwhile approach to quantifying potential impacts of ocean acidification, warming, and/or deteriorating water quality on the process of calcification.

Florida

Advances in coral immunity ‘omics in response to disease outbreaks

Coral disease has progressively become one of the most pressing issues affecting coral reef survival. In the last 50 years, several reefs throughout the Caribbean have been severely impacted by increased frequency and intensity of disease outbreaks leading to coral death. A recent example of this is stony coral tissue loss disease which has quickly spread throughout the Caribbean, devastating coral reef ecosystems. Emerging from these disease outbreaks has been a coordinated research response that often integrates ‘omics techniques to better understand the coral immune system. ‘Omics techniques encompass a wide range of technologies used to identify large scale gene, DNA, metabolite, and protein expression. In this review, we discuss what is known about coral immunity and coral disease from an ‘omics perspective. We reflect on the development of biomarkers and discuss ways in which coral disease experiments to test immunity can be improved. Lastly, we consider how existing data can be better leveraged to combat future coral disease outbreaks.

Frontiers in Marine Science

International Ocean Discovery Program Expedition 389 preliminary report: Hawaiian drowned reefs

Our understanding of the mechanisms controlling eustatic sea level and global climate changes has been hampered by a lack of appropriate fossil coral records over the last 500 ky, particularly into and out of the glacial periods. This problem was addressed by International Ocean Discovery Program Expedition 389 by drilling a unique succession of Hawaiian drowned coral reefs now at 110–1300 meters below sea level (mbsl). The four objectives are to investigate (1) the timing, rate, and amplitude of sea level variability to examine cryosphere and geophysical processes, including the assessment of abrupt sea level change events; (2) the processes that determine changes in mean and high-frequency (seasonal–interannual) climate variability from times with different boundary conditions (e.g., ice sheet size, p CO 2 , and solar forcing); (3) the response of coral reef systems to abrupt sea level and climate changes; and (4) the variation through space and time of the subsidence and the volcanic evolution of the island. To achieve these objectives, 35 holes at 16 sites ranging 131.9–1241.8 mbsl were drilled during the expedition. A total of 425 m of core was recovered, comprising reef (83%) and volcanic (17%) material. Average core recoveries were 66%, with numerous intervals characterized by very well preserved mixtures of coralgal and microbialite frameworks with recoveries >90%. Some science-critical shallow sites were not drilled due to a failure to secure permits to operate in Hawaiian state waters. Furthermore, apart from one site the target penetration depths were not achieved. Preliminary radiometric dates indicate that the recovered reef deposits are from 488 to 13 ka in age. The Onshore Science Party took place in February 2024. Cores were CT and hyperspectral scanned and described. Standard measurements were made, and samples were taken for postcruise research. Preliminary assessment of the age and quality of the reef and basalt cores suggest that many of the expedition objectives will be met.

Hawaii

Augmentation of French grunt diet description using combined visual and DNA-based analyses

Trophic linkages within a coral-reef ecosystem may be difficult to discern in fish species that reside on, but do not forage on, coral reefs. Furthermore, dietary analysis of fish can be difficult in situations where prey is thoroughly macerated, resulting in many visually unrecognisable food items. The present study examined whether the inclusion of a DNA-based method could improve the identification of prey consumed by French grunt, Haemulon flavolineatum, a reef fish that possesses pharyngeal teeth and forages on soft-bodied prey items. Visual analysis indicated that crustaceans were most abundant numerically (38.9%), followed by sipunculans (31.0%) and polychaete worms (5.2%), with a substantial number of unidentified prey (12.7%). For the subset of prey with both visual and molecular data, there was a marked reduction in the number of unidentified sipunculans (visual – 31.1%, combined &ndash 4.4%), unidentified crustaceans (visual &ndash 15.6%, combined &ndash 6.7%), and unidentified taxa (visual &ndash 11.1%, combined &ndash 0.0%). Utilising results from both methodologies resulted in an increased number of prey placed at the family level (visual &ndash 6, combined &ndash 33) and species level (visual &ndash 0, combined &ndash 4). Although more costly than visual analysis alone, our study demonstrated the feasibility of DNA-based identification of visually unidentifiable prey in the stomach contents of fish.

Marine and Freshwater Research