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Differential changes in the onset of spring across US National Wildlife Refuges and North American migratory bird flyways

Warming temperatures associated with climate change can have indirect effects on migratory birds that rely on seasonally available food resources and habitats that vary across spatial and temporal scales. We used two heat-based indices of spring onset, the First Leaf Index (FLI) and the First Bloom Index (FBI), as proxies of habitat change for the period 1901 to 2012 at three spatial scales: the US National Wildlife Refuge System; the four major bird migratory flyways in North America; and the seasonal ranges (i.e., breeding and non-breeding grounds) of two migratory bird species, Blue-winged Warbler ( Vermivora cyanoptera ) and Whooping Crane ( Grus americana ). Our results show that relative to the historical range of variability, the onset of spring is now earlier in 76% of all wildlife refuges and extremely early (i.e., exceeding 95% of historical conditions) in 49% of refuges. In all flyways but the Pacific, the rate of spring advance is generally greater at higher latitudes than at lower latitudes. This differential rate of advance in spring onset is most pronounced in the Atlantic flyway, presumably because of a “warming hole” in the southeastern US. Both FLI and FBI have advanced markedly in the breeding ranges–but not the non-breeding ranges–of the two selected bird species, albeit with considerable intra-range variation. Differences among species in terms of migratory patterns and the location and extent of seasonal habitats, as well as shifts in habitat conditions over time, may complicate predictions of the vulnerability of migratory birds to climate change effects. This study provides insight into how differential shifts in the phenology of disparate but linked habitats could inform local- to landscape-scale management strategies for the conservation of migratory bird populations.

PLoS ONE

Changes in the prevalence of avian disease and mosquito vectors at Hakalau Forest National Wildlife Refuge: a 14-year perspective and assessment of future risk

Throughout the main Hawaiian Islands, introduced mosquito-borne disease has had, and continues to have, a profound impact on the distributions and abundance of native Hawaiian forest birds. Populations of remaining native forest birds are largely restricted to high elevation forests where mean temperatures are marginal for vector and parasite development and limited availability of larval mosquito habitat constrains mosquito populations and disease transmission. Hakalau Forest National Wildlife Refuge (HFNWR) was established for the preservation of endemic avifauna in 1985. Since its creation, native bird communities there have remained intact and most species populations are stable or increasing. However, avian malaria had been detected at HFNWR in the past and, in light of documented climate change, new concerns have been raised regarding the long-term fate of the refuge’s forest birds. To examine the possible changes in avian malaria transmission at HFNWR we sampled forest birds for blood parasites, trapped adult mosquitoes and surveyed larval mosquito habitat at three sites during 2012 and compared our results with similar data collected between 1998 and 1999. We tested blood samples by polymerase chain reaction (PCR), immunoblotting, and microscopy to determine prevalence of acute and chronic infection and used attractive gravid traps to sample the vector mosquito Culex quinquefasciatus. Our study documented spatial trends and temporal changes in the prevalence of avian malaria, mosquito presence, larval mosquito habitat and feral pig activity at HFNWR. We found evidence of local transmission in high elevation forests, a general pattern of increasing prevalence at lower elevations and along a South to North gradient and a two-fold decrease in the prevalence of avian malaria in the intervening 14 years. Despite considerable effort, we were unable to detect larval C. quinquefasciatus and captured only one adult indicating that the vector of avian malaria has a very limited presence at HFNWR. We did, however, document the establishment of another invasive mosquito, Aedes japonicus japonicus , and its occurrence in tree fern cavities and rock pools as larval habitat in the lower forests of HFNWR. We suggest that interspecific competition by A. j. japonicus and predation by a suite of native predators may provide biotic resistance to the establishment of permanent C. quinquefasciatus populations. While current predictions of climate change in the Hawaiian Islands include a gradual warming and enhanced transmission by mid-century, the current cooling trend recorded at high elevation HFNWR illustrates the importance of monitoring to document fine scale temporal and site specific changes in prevalence. Long term changes in precipitation may have a more profound effect on local transmission of malaria than temperature and we may have already seen some potential impacts of an extended drought at HFNWR with a decrease in feral pig activity and pig associated larval mosquito habitat and increases in stream-associated larval mosquito habitat.

Hawaii

Host plant associations of Lepidoptera and implications for forest bird management at Hakalau Forest National Wildlife Refuge

Forests dominated or co-dominated by ‘ōhi‘a ( Metrosideros polymorpha ) are critical to most Hawaiian forest birds, but fungal diseases causing Rapid ‘Ōhi‘a Death (ROD) threaten ‘ōhi‘a-based food webs that support native bird communities on Hawai‘i Island. Caterpillars are the most frequently consumed arthropod prey of native birds and their young and are especially frequent in the diets of one threatened (T) and three endangered (E) species (“listed” species) at Hakalau Forest National Wildlife Refuge (Hakalau): ‘akiapōlā‘au ( Hemignathus wilsoni , E), ‘alawī (Hawai‘i creeper; Loxops mana , E), Hawai‘i ‘ākepa ( L. coccineus , E), and ‘i‘iwi ( Drepanis coccinea , T). Hakalau harbors the largest and most stable populations of listed forest birds in Hawai‘i, presumably due to the availability of food resources and the extent of suitable, managed habitat above the range of mosquito-borne avian malaria. Because a previous study indicated that only a few caterpillar species were important in the diets of listed birds at Hakalau, we investigated the distribution of caterpillars on common host plants available to foraging birds. Eleven native plant species hosted two or more taxa identified to genus or species, with at least seven from ‘ōhi‘a, six from koa ( Acacia koa ), and five from ‘ākala ( Rubus hawaiensis ). We identified 16 taxa to genus or species from 9 families, assigning 11 to species. Leaves, which were the focus of our sampling effort, were the substrate used by 20 caterpillar taxa, and dead wood or bark was used by 7 taxa. In a previous study, we classified 19 morphotypes of caterpillar mandibles in the diets of native and alien birds at Hakalau, and in the present study we dissected mandibles from caterpillars that likely matched 10 of those morphotypes. These 10 morphotypes potentially represented >95% of caterpillar prey found in the earlier diet study and were collected from 11 host plant species, with ‘ōhi‘a hosting 8 morphotypes, 4 of which were exclusive to ‘ōhi‘a. The most widely hosted morphotype was found on all 11 plant species that we sampled, including ‘ōhi‘a, but the other 9 morphotypes were found on 1–7 hosts. As shown by the previous diet study, each of the listed bird species consumed caterpillar prey consisting mostly of combinations of two morphotypes drawn from a pool of only five, indicating a high degree of specialization. In the present study, we collected three of the five key morphotypes only on ‘ōhi‘a, highlighting the importance of this tree to listed bird species. Because ‘ōhi‘a forests in Hakalau remain vulnerable to ROD, measures to mitigate the impacts of reduced ‘ōhi‘a cover are important to consider from the perspective of forest bird food webs and diet. Ongoing reforestation of former pasturelands with koa and common understory species should provide alternative caterpillar prey for forest birds. Our results and information from the literature indicate that koa supports, to varying degrees, nearly all forest birds at Hakalau, while ‘ākala, ‘ōhelo ( Vaccinium calycinum ), kōlea ( Myrsine lessertiana ), ‘ōlapa ( Cheirodendron trigynum ), pūkiawe ( Leptecophylla tameiameiae ), and māmaki ( Pipturus albidus ) could benefit bird populations by increasing prey availability and structural complexity in koa-dominated stands. Foraging studies and additional research to identify species and host plant associations of important forest bird prey, including caterpillars and other arthropods, can help managers evaluate the complex interactions between native forest birds and their food webs and habitats.

Hawaii

Social attraction used to establish Caspian tern (Hydroprogne caspia) nesting colonies on modified islands at the Don Edwards San Francisco Bay National Wildlife Refuge, California—Final report

To address the 2008/2010 and Supplemental 2014 National Oceanic and Atmospheric Administration Fisheries Biological Opinion for operation of the Federal Columbia River Power System, the U.S. Army Corps of Engineers (USACE) and the Bureau of Reclamation (Reclamation) developed and began implementation of Caspian tern ( Hydroprogne caspia ) management plans. This implementation includes redistribution of the Caspian terns in the Columbia River estuary and the mid-Columbia River region to reduce predation on salmonids listed under the Endangered Species Act. Key elements of the plans are (1) reduction of nesting habitat for Caspian terns in the Columbia River estuary and the mid-Columbia River region, and (2) creation or modification of nesting habitat at alternative sites within the Caspian tern breeding range. As part of this effort, USACE and Reclamation developed Caspian tern nesting habitat at the U.S. Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge (DENWR), California, prior to the 2015 nesting season. Furthermore, nesting habitat for western snowy plovers ( Charadrius alexandrinus nivosus ) also was developed to provide separate nesting opportunities in the same managed ponds to reduce potential conflicts with Caspian terns. Specifically, seven recently constructed islands within two managed ponds (Ponds A16 and SF2) of DENWR were modified to provide habitat attractive to nesting Caspian terns (5 islands) and snowy plovers (2 islands). These 7 islands were a subset of 46 islands recently constructed in Ponds A16 and SF2 to provide waterbird nesting habitat as part of the South Bay Salt Pond (SBSP) Restoration Project. We used social attraction methods (decoys and electronic call systems) to attract Caspian terns and snowy plovers to these seven modified islands, and conducted surveys from March to September of 2015, 2016, and 2017 to evaluate nest numbers, nest density, and productivity. Results from the 2015 nesting season, the first year of the study, indicated that island modifications and social attraction measures were successful in establishing Caspian tern breeding colonies at Ponds A16 and SF2 of DENWR. Prior to 2015, there was no history of Caspian terns nesting in either Pond A16 or Pond SF2. The success of 2015 continued in 2016 and 2017. In 2017, the third and final year of the project, Caspian terns initiated at least 664 nests, fledged at least 239 chicks, and had a breeding success rate of 0.36 fledged chicks per breeding pair. This represents a 171 percent increase in the number of breeding pairs and a 41 percent increase in the number of chicks fledged, but a 48 percent decrease in the fledglings produced per breeding pair in 2017 compared to 2015, the first year the colonies were established. The two new large and growing Caspian tern nesting colonies at Ponds A16 and SF2 demonstrate the effectiveness of social attraction measures in helping to establish tern nesting colonies in San Francisco Bay. Social attraction measures similar to those used in this study, but targeting other colonial species such as Forster’s terns ( Sterna forsteri ) and American avocets ( Recurvirostra americana ), may help to establish waterbird breeding colonies at wetlands enhanced as part of the SBSP Restoration Project.

California

Arthropod community structure on bark of koa ( Acacia koa ) and ʻōhiʻā ( Metrosideros polymorpha ) at Hakalau Forest National Wildlife Refuge, Hawaiʻi Island, Hawaiʻi

The arthropod community associated with tree bark contains a wide variety of taxa but is poorly described, particularly in Hawaiʽi. Our overall goals were to evaluate the abundance of arthropods available to foraging birds and how variation in bark substrates may contribute to arthropod distributions in native forests. Our study aimed to identify this fauna on the dominant canopy-forming trees koa ( Acacia koa ) and ʽōhiʽa ( Metrosideros polymorpha ) within wet montane forest at Hakalau Forest National Wildlife Refuge, Hawaiʽi Island. At two sites roughly similar in elevation and habitat structure, we deployed three trap types designed to intercept arthropods moving along bark within tree canopies: a bole trap based on a pre-existing design and two traps specially designed for this study. Bole traps were placed on koa and ʽōhiʽa while branch traps were established on large and small branches of ʽōhiʽa. In total, 15 arthropod orders were identified, with Collembola most abundant (number/trap-day) generally followed by Isopoda and Araneae. Differences in abundance were found in some instances, but overall, few differences were detected between tree species or sites. Relative abundances of arthropod groups were also generally similar between trees and sites and among different parts of ʽōhiʽa. These results indicate that bark-dwelling arthropod communities are similar on koa and ʽōhiʽa, and birds should not develop strong preferences for gleaning arthropods from the bark of either species of tree based on prey availability.

Hawai'i

Soil data for a collapse-scar bog chronosequence in Koyukuk Flats National Wildlife Refuge, Alaska, 2008

Peatlands in the northern permafrost region store large amounts of organic carbon, most of which is currently stored in frozen peat deposits. Recent warming at high-latitudes has accelerated permafrost thaw in peatlands, which will likely result in the loss of soil organic carbon from previously frozen peat deposits to the atmosphere. Here, we report soil organic carbon inventories, soil physical data, and field descriptions from a collapse-scar bog chronosequence located in a peatland ecosystem at Koyukuk Flats National Wildlife Refuge in Alaska.

Alaska

Response of vegetation in open and partially wooded fens to prescribed burning at Seney National Wildlife Refuge

The health and function of northern peatlands, particularly for fens, are strongly affected by fire and hydrology. Fens are important to several avian species of conservation interest, notably the yellow rail ( Coturnicops noveboracensis ). Fire suppression and altered hydrology often result in woody encroachment, altering the plant community and structure. Woody encroachment and its effects on biodiversity have become an increasing concern in the conservation and management of plant communities. This study evaluated the effects of spring and summer prescribed burns on the plant community, cover, and structure in open and partially wooded fens at Seney National Wildlife Refuge, Michigan, using a before-after-control-impact design. Paired, 1-hectare blocks were established in two fen areas, C3 and Marsh Creek, and data were collected for 2 years before burning (2006–7) and 3 years after burning (2008–10). We used generalized linear mixed models and ordination to assess differences among four treatments: C3 control, C3 spring burn (May 2008), Marsh Creek control, and Marsh Creek summer burn (July 2008); results from a block burned under drier conditions in July 2007 also are reported. Variables include water depth; litter depth; graminoid height; species richness and diversity; percent cover of plant taxa, mosses, and open area; shrub height, number of patches, and cover; and visual obstruction readings. The 2008 prescribed burns were done under moderate fire conditions, whereas the 2007 summer burn on one block was done under high fire conditions because of prolonged drought. We identified 104 plant taxa over the 5 years and noted differences between C3 and Marsh Creek communities. We examined data for effects of treatment, year, and year × treatment interactions for percent open and the 28 most common taxa. Most differences among treatments were related to natural differences in the plant community and hydrology between the two areas rather than fire effects; year effects were likely related to annual differences in water conditions. We detected few effects of spring burning in C3, even in the same year of burning. In Marsh Creek, most treatment effects were in 2008, when data were collected within 3 weeks of burning. Some fire effects there, however, persisted one to two growing seasons (2009, 2010) and two to three growing seasons in the block burned in the more intense summer 2007 fire. Effects of burning on shrub measures were more apparent on summer-burned blocks, but most measures returned to preburn conditions by 2010. Our results demonstrate the heterogeneity of plant community and environmental conditions of fens within and among years and the interactions of water conditions with burning. The results also demonstrate that neither single spring nor summer burning under moderate fire conditions are effective in setting back woody cover. Maintaining more open conditions in fens may require different approaches to water management, more frequent fires, more aggressive fire management, or a combination of tools to control woody cover.

Michigan

Hydrologic conditions in the Bill Williams River National Wildlife Refuge and Planet Valley, Arizona, 2000

During a period of sustained base-flow conditions in the Bill Williams River below Alamo Dam in west central Arizona from March to July 2000, the channel of the river through Planet Valley was dry, and the water table sloped almost due west parallel to the main slope of the flood plain. Water from the river infiltrated into the channel bottom at the head of Planet Valley, moved downgradient in the subsurface, and reappeared in the channel about 0.3 mile downstream from the east boundary of the Bill Williams River National Wildlife Refuge. A river aquifer in hydraulic connection with the Bill Williams River was mapped from a point 6.3 miles upstream from Highway 95 to the upstream end of Planet Valley. Formations that make up the river aquifer in Planet Valley are younger alluvium, older alluviums, and fanglomerate. Total thickness of the river aquifer probably is less than 200 feet in the bedrock canyons to as much as 1,035 feet in Planet Valley. The purpose of this study was to investigate the current hydrologic conditions along the Bill Williams River, which included an inventory of wells within the river aquifer of the Colorado River and in Planet Valley, and to determine the configuration of the water table. A map shows the elevation and configuration of the water table from the east end of Planet Valley to the confluence of the Bill Williams River with Lake Havasu.

Arizona

Aerial dispersal of Lygodium microphyllum spores within Arthur R. Marshall Loxahatchee National Wildlife Refuge

Native across the Old World tropics from Africa to Southeast Asia and Australia, Lygodium microphyllum (Cav.) R. Br. (Old World Climbing Fern) is one of the most invasive plant species threatening South Florida ecosystems (Rodgers et al. 2014). This invasive fern was first collected as a naturalized plant in South Florida in the late 1960s (Beckner 1968). Subsequent populations were observed in the late 1970s in both Martin and Palm Beach Counties (Nauman and Austin 1978). In 1989 L. microphyllum was observed by the South Florida Water Management District (SFWMD) in the Arthur R. Marshall Loxahatchee National Wildlife Refuge (LNWR). In 1995, a survey by SFWMD indicated that 12% of LNWR was colonized by L. microphyllum. By 1997 this had increased to 36% (Ferriter 2001). Ten years later, Systematic Reconnaissance Flights conducted over LNWR estimated that 44% of LNWR had sparse (> a single individual tree, shrub or stem to <50%) or dense (>50%) infestations of L. microphyllum

Florida Scientist

Optimal water depth management on river-fed National Wildlife Refuges in a changing climate

The prairie pothole region (PPR) in the north-central United States and south-central Canada constitutes the most important waterfowl breeding area in North America. Projected long-term changes in precipitation and temperature may alter the drivers of waterfowl abundance: wetland availability and emergent vegetation cover. Previous studies have focused on isolated wetland dynamics, but the implications of changing precipitation on managed, river-fed wetlands have not been addressed. Using a structured decision making (SDM) approach, we derived optimal water management actions for 20 years at four river-fed National Wildlife Refuges (NWRs) in North and South Dakota under contrasting increasing/decreasing (+/&minus;0.4 %/year) inflow scenarios derived from empirical trends. Refuge pool depth is manipulated by control structures. Optimal management involves setting control structure heights that have the highest probability of providing a desired mix of waterfowl habitat, given refuge capacities and inflows. We found optimal seasonal control structure heights for each refuge were essentially the same under increasing and decreasing inflow trends of 0.4 %/year over the next 20 years. Results suggest managed pools in the NWRs receive large inflows relative to their capacities. Hence, water availability does not constrain management; pool bathymetry and management tactics can be greater constraints on attaining management objectives than climate-mediated inflow. We present time-dependent optimal seasonal control structure heights for each refuge, which are resilient to the non-stationary precipitation scenarios we examined. Managers can use this information to provide a desired mixture of wildlife habitats, and to re-assess management objectives in reserves where pool bathymetry prevents attaining the currently stated objectives.

Climatic Change

Dynamics and ecological consequences of the 2013−2014 koa moth outbreak at Hakalau Forest National Wildlife Refuge.

A massive outbreak of the koa moth (Geometridea: Scotorythra paludicola) defoliated more than a third of the koa (Acacia koa) forest on Hawai&lsquo;i Island during 2013&minus;2014. This was the largest koa moth outbreak ever recorded and the first on the island since 1953. The outbreak spread to sites distributed widely around the island between 800&minus;2,000 m elevation and in wet rainforest to dry woodland habitats. We monitored the outbreak at two windward forest sites (Laupāhoehoe and Saddle Road Kīpuka) and one leeward forest site (Kona), and we studied the dynamics of the outbreak and its impacts on the forest ecosystem at Hakalau Forest National Wildlife Refuge, our higher elevation windward site. Study sites at Hakalau included two stands of koa that were planted (reforestation stands) in former cattle pastureland about 20 years earlier and two stands of koa that were dominated by &lsquo;ōhi&lsquo;a (Metrosideros polymorpha) and that were naturally recovering from cattle grazing (forest stands). We observed one outbreak at Hakalau, multiple outbreaks at the two other windward sites, but no outbreak at the leeward site. Caterpillars at Hakalau reached peak estimated abundances of more than 250,000 per tree and 18,000,000 per hectare, and they removed between 64&minus;93% of the koa canopy in managed forest stands. Defoliation was more extensive in naturally recovering forest, where &lsquo;ōhi&lsquo;a dominated and koa was less abundant, compared to the planted stands, where koa density was high. Koa trees were still growing new foliage six months after being defoliated, and leaves were produced in greater proportion to phyllodes, especially by small koa (&le; 8 cm dbh) and by larger trees in forest stands, where light levels may have remained relatively low after defoliation due to the high cover of &lsquo;ōhi&lsquo;a. Small branches of many trees apparently died, and canopy regrowth was absent or low in 9% of koa trees and seedlings, which indicates the likely level of mortality. Between 2,000&minus;5,000 kg/ha of frass fell during the defoliation event, resulting in the deposition of up to 200 kg/ha of highly labile nitrogen on the forest floor in less than two months. The deposition of nitrogen was detected as pulses in resin-available nitrogen in the top 5&minus;10 cm of soil at two of three sites. These sites showed elevated soil nitrogen for about seven months. Nitrogen content of understory plant foliage, which is indicative of nitrogen uptake, suggested weak and variable effects of nitrogen deposition in the soil. Foliar nitrogen increased slightly in alien pasture grasses four months after the deposition of frass, although distinctive increases were not detected in native woody species. Birds responded to the abundance of caterpillars by increasing their activity in koa during the buildup of caterpillars and decreasing their use of koa after defoliation. During the outbreak, caterpillars increased in the diets of the two generalist insectivores we examined, and nearly all species gained weight. Bats responded to the abundance of moths by compression of active foraging into the first three hours of darkness each night after presumably having reached a digestive bottleneck. Reduced foraging activity by bats also resulted in lower indices of detectability based upon acoustic monitoring when compared to non-outbreak years. Parasitoid wasps tracked caterpillar abundance, but the low rate at which they attacked caterpillars suggests that they had little influence on the population. The predatory yellowjacket (Vespula pensylvanica) did not respond to the outbreak. Although a single, protracted outbreak occurred at Hakalau, multiple outbreaks and defoliations occurred at lower elevations. Our results provide a broad foundation for evaluating the dynamics and impacts of future Scotorythra outbreaks.

Hawaii

Landscape unit based digital elevation model development for the freshwater wetlands within the Arthur C. Marshall Loxahatchee National Wildlife Refuge, Southeastern Florida

The hydrologic regime is a critical limiting factor in the delicate ecosystem of the greater Everglades freshwater wetlands in south Florida that has been severely altered by management activities in the past several decades. "Getting the water right" is regarded as the key to successful restoration of this unique wetland ecosystem. An essential component to represent and model its hydrologic regime, specifically water depth, is an accurate ground Digital Elevation Model (DEM). The Everglades Depth Estimation Network (EDEN) supplies important hydrologic data, and its products (including a ground DEM) have been well received by scientists and resource managers involved in Everglades restoration. This study improves the EDEN DEMs of the Loxahatchee National Wildlife Refuge, also known as Water Conservation Area 1 (WCA1), by adopting a landscape unit (LU) based interpolation approach. The study first filtered the input elevation data based on newly available vegetation data, and then created a separate geostatistical model (universal kriging) for each LU. The resultant DEMs have encouraging cross-validation and validation results, especially since the validation is based on an independent elevation dataset (derived by subtracting water depth measurements from EDEN water surface elevations). The DEM product of this study will directly benefit hydrologic and ecological studies as well as restoration efforts. The study will also be valuable for a broad range of wetland studies.

Florida

Spatial heterogeneity in statistical power to detect changes in lake area in Alaskan National Wildlife Refuges

Over the past 50 years, the number and size of high-latitude lakes have decreased throughout many regions; however, individual lake trends have been variable in direction and magnitude. This spatial heterogeneity in lake change makes statistical detection of temporal trends challenging, particularly in small analysis areas where weak trends are difficult to separate from inter- and intra-annual variability. Factors affecting trend detection include inherent variability, trend magnitude, and sample size. In this paper, we investigated how the statistical power to detect average linear trends in lake size of 0.5, 1.0 and 2.0 %/year was affected by the size of the analysis area and the number of years of monitoring in National Wildlife Refuges in Alaska. We estimated power for large (930–4,560 sq km) study areas within refuges and for 2.6, 12.9, and 25.9 sq km cells nested within study areas over temporal extents of 4–50 years. We found that: (1) trends in study areas could be detected within 5–15 years, (2) trends smaller than 2.0 %/year would take >50 years to detect in cells within study areas, and (3) there was substantial spatial variation in the time required to detect change among cells. Power was particularly low in the smallest cells which typically had the fewest lakes. Because small but ecologically meaningful trends may take decades to detect, early establishment of long-term monitoring will enhance power to detect change. Our results have broad applicability and our method is useful for any study involving change detection among variable spatial and temporal extents.

Alaska

Dust control products at Hagerman National Wildlife Refuge, Texas: environmental safety and performance

Controlling fugitive dust while protecting natural resources is a challenge faced by all managers of unpaved roads. Unfortunately, road managers choosing between dust control products often have little objective environmental information to aid their decisions. To address this information gap, the U.S. Geological Survey and the U.S. Fish and Wildlife Service collaborated on a field test of three dust control products with the objectives of ( a ) evaluating product performance under real-world conditions, ( b ) verifying the environmental safety of products identified as practically nontoxic in laboratory tests, and ( c ) testing the feasibility of several environmental monitoring techniques for use in dust control tests. In cooperation with refuge staff and product vendors, three products (one magnesium chloride plus binder, one cellulose, and one synthetic fluid plus binder) were applied in July 2012 to replicated road sections at the Hagerman National Wildlife Refuge in Texas. These sections were monitored periodically for 12 months after application. Product performance was assessed by mobile-mounted particulate-matter meters measuring production of fugitive dust and by observations of road conditions. Environmental safety was evaluated through on-site biological observations and leaching tests with samples of treated aggregate. All products reduced dust and improved surface condition during those 12 months. Planned environmental measurements were not always compatible with day-to-day refuge management actions; this incompatibility highlighted the need for flexible biological monitoring plans. As one of the first field tests of dust suppressants that explicitly incorporated biological endpoints, this effort provides valuable information for improving field tests and for developing laboratory or semifield alternatives.

Texas

Prevalence of the amphibian chytrid fungus (Batrachochytrium dendrobatidis) at Buenos Aires National Wildlife Refuge, Arizona, USA

Information on disease presence can be of use to natural resource managers, especially in areas supporting threatened and endangered species that occur coincidentally with species that are suspected vectors for disease. Ad hoc reports may be of limited utility (Muths et al. 2009), but a general sense of pathogen presence (or absence) can inform management directed at T&E species, especially in regions where disease is suspected to have caused population declines (Bradley et al. 2002). The Chiricahua Leopard Frog (Lithobates chiricahuensis), a species susceptible to infection by the amphibian chytrid fungus (Batrachochytrium dendrobatidis, Bd) (Bradley et al. 2002), and the non-native, invasive American Bullfrog (L. catesbeianus), a suspected vector for chytridiomycosis (Schloegel et al. 2012, Gervasi et al. 2013), both occur at Buenos Aires National Wildlife Refuge (BANWR) and surrounding lands in southern Arizona. Efforts to eradicate the bullfrog from BANWR began in 1997 (Suhre, 2010). Eradication from the southern portion of BANWR was successful by 2008 but the bullfrog remains present at the Arivaca Cienega and in areas immediately adjacent to the refuge (Fig. 1). Curtailing the re-invasion of the bullfrog into BANWR will require vigilance as to ensure the health of Chiricahua Leopard Frog populations.

Arizona

Archive of bathymetry and backscatter data collected in 2014 nearshore Breton and Gosier Islands, Breton National Wildlife Refuge, Louisiana

As part of the Barrier Island Monitoring Project, scientists from the U.S. Geological Survey (USGS) St. Petersburg Coastal and Marine Science Center conducted nearshore geophysical surveys off Breton and Gosier Islands, Louisiana, in July and August of 2014. To assist the United States Fish and Wildlife Service (USFWS) with restoration planning efforts, the USGS was tasked with answering fundamental questions about the physical environment of the southern Chandeleur Islands, including the geology, morphology, and oceanography. Baseline data needed to answer these questions were either insufficient or missing. The USGS conducted a comprehensive geologic investigation in the summer of 2014, collecting geophysical and sedimentological data. Breton Island, located at the southern end of the Chandeleur Island chain in southeastern Louisiana, was recognized as a natural, globally significant nesting sanctuary for several bird species and was established as the Breton National Wildlife Refuge (NWR) in 1904. The areal extent of Breton Island has diminished 90 percent since 1920. Land loss is attributed to ongoing relative sea-level rise, diminished sediment supply, and storm impacts. The bird population on Breton Island has also declined over the years, most notably after Hurricane George in 1998 and after Hurricane Katrina in 2015; the latter completely submerged the island. Despite decreasing habitable acreage, migratory seabirds continue to return and nest on Breton Island. To prevent the island from being submerged in the future, and to protect, stabilize, and provide more nesting and foraging areas for the bird population, the USFWS proposed a restoration effort to rebuild Breton Island to its pre-Katrina footprint. This data series serves as an archive of processed interferometric swath and single-beam bathymetry data, and side-scan sonar data, collected in the nearshore of Breton and Gosier Islands, NWR, Louisiana. The data were collected during two USGS cruises (USGS Field Activity Numbers 2014-314-FA and 2014-317-FA) in July and August 2014. Geographic information system data products include a 100-meter-cell-size interpolated bathymetry grid, trackline maps, and point data files. Additional files include error analysis maps, Field Activity Collection System logs, and formal Federal Geographic Data Committee metadata. NOTE: These data are scientific in nature and are not to be used for navigation. Any use of trade names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Louisiana

Efficacy of eDNA as an early detection indicator for Burmese pythons in the ARM Loxahatchee National Wildlife Refuge in the Greater Everglades Ecosystem

Environmental DNA (eDNA) detection of invasive species can be used to delimited occupied ranges and estimate probabilities to inform management decisions. Environmental DNA is shed into the environment through skin cells and bodily fluids and can be detected in water samples collected from lakes, rivers, and swamps. In south Florida, invasive Burmese pythons occupy much of the Greater Everglades in mostly inaccessible habitat and are credited with causing severe declines of native species’ populations. Detection of Burmese pythons by traditional methods, such as trapping and visual searching, have been largely ineffective, making eDNA a superior method for differentiating invaded habitat. We adapted a quantitative PCR eDNA assay for droplet digital PCR, a state-of-the-art method that improves precision and accuracy. From August 2014 to October 2016, locations in and around Arthur R. Marshall Loxahatchee National Wildlife Refuge in southeast Florida were surveyed for Burmese python eDNA. The Refuge is maintained to provide water storage and is considered one of the last remnants of the northern Everglades wetlands. Positive eDNA detections were made at each of the five sampling events, assessing a total of 399 samples, with moderate occurrence (ψ=58-91%) and detection (p=40-70%) probabilities, potentially reduced by high PCR inhibition-levels. The high occurrence rates and geographic distribution of the positive samples within the Refuge suggests a steady release of python eDNA from a resident Burmese python population and reduces support for primarily transport of eDNA through boats or flowing water from the north. The first confirmed sighting of a Burmese python in the Refuge occurred in September 2016, after eDNA testing had indicated the presence of pythons. An established population is not expected this far north, however, the detections likely indicate northern range limit of a consistent population at Loxahatchee on the eastern side of the Florida peninsula. Our study demonstrates the benefit of eDNA for determining more accurate range limits and expansion information for Burmese pythons, as well as laying the foundation for the assessment of control efforts.

Florida

Geology and mineral resources of the Sheldon-Hart Mountain National Wildlife Refuge Complex (Oregon and Nevada), the Southeastern Oregon and North-Central Nevada, and the Southern Idaho and Northern Nevada (and Utah) Sagebrush Focal Areas: Chapter B in Mineral resources of the Sagebrush Focal Areas of Idaho, Montana, Nevada, Oregon, Utah, and Wyoming

Summary The U.S. Department of the Interior has proposed to withdraw approximately 10 million acres of Federal lands from mineral entry (subject to valid existing rights) from 12 million acres of lands defined as Sagebrush Focal Areas (SFAs) in Idaho, Montana, Nevada, Oregon, Utah, and Wyoming (for further discussion on the lands involved see Scientific Investigations Report 2016–5089–A). The purpose of the proposed action is to protect the greater sage-grouse ( Centrocercus urophasianus ) and its habitat from potential adverse effects of locatable mineral exploration and mining. The U.S. Geological Survey Sagebrush Mineral-Resource Assessment (SaMiRA) project was initiated in November 2015 and supported by the Bureau of Land Management to (1) assess locatable mineral-resource potential and (2) to describe leasable and salable mineral resources for the seven SFAs and Nevada additions. This chapter summarizes the current status of locatable, leasable, and salable mineral commodities and assesses the potential of selected locatable minerals in lands proposed for withdrawal that span the Nevada, Oregon, Idaho, and Utah borders. In this report, the four study areas evaluated were (1) the Sheldon-Hart Mountain National Wildlife Refuge Complex SFA in Washoe County, Nevada, and Harney and Lake Counties, Oregon; (2) the Southeastern Oregon and North-Central Nevada SFA in Humboldt County, Nevada, and Harney and Malheur Counties, Oregon; (3) the Southern Idaho and Northern Nevada SFA in Cassia, Owyhee, and Twin Falls Counties, Idaho, Elko County, Nevada, and Box Elder County, Utah; and (4) the Nevada additions in Humboldt and Elko Counties, Nevada.

Idaho, Nevada, Oregon, Utah