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At least 271 records · Page 15Linked to original sources
USGS2025

Combining scanning electron microscopy, X-ray diffraction, and X-ray fluorescence to characterize shear zones at the Pogo gold deposit, Alaska

This study employs a multi-method analytical approach to characterize the mineralogical, geochemical, and textural properties of fault rocks from the Pogo gold mine in the Yukon-Tanana Upland, central Alaska. Specifically, we examine cataclasites, to document the structural and geochemical evolution of shear zones and their associations with gold mineralization. To investigate the shear zone, we integrate portable X-ray fluorescence (pXRF), scanning electron microscopy-based automated mineralogy (SEM-AM), X-ray diffraction (XRD), and high-resolution micro-X-ray fluorescence (micro-XRF) mapping. These methods collectively provide insights into bulk and trace element chemistry, mineralogical composition, and deformation-related textures across multiple scales. Handheld pXRF enables rapid geochemical screening, guiding SEM-AM and XRD analyses to ensure consistent mineralogical interpretation. X-ray diffraction identifies and quantifies crystalline phases, while SEM-AM produces high-resolution mineral maps, revealing mineral abundances, grain-scale textures, and gold associations. Micro-XRF mapping further refines our understanding by showing visual trace element distributions at sub-millimetre resolution. By integrating these techniques, we improve our understanding of the nature and geochemistry of Pogo shear zones, their role in gold mineralization, and support metallurgical processing strategies. This approach enhances exploration models and resource characterization for structurally complex gold deposits.

Alaska↗
USGS1989

Can we determine the biological availability of sediment-bound trace elements?

It is clear from available data that the susceptibility of biological communities to trace element contamination differs among aquatic environments. One important reason is that the bioavailability of metals in sediments appears to be altered by variations in sediment geochemistry. However, methods for explaining or predicting the effect of sediment geochemistry upon metal bioavailability are poorly developed. Experimental studies demonstrate that ingestion of sediments and uptake from solution may both be important pathways of metal bioaccumulation in deposit/detritus feeding species. Relative importance between the two is geochemistry dependent. Geochemical characteristics of sediments also affect metal concentrations in the tissues of organisms collected from nature, but the specific mechanisms by which these characteristics influence metal bioavailability have not been rigorously demonstrated. Several prerequisites are necessary to better understand the processes that control metal bioavailability from sediments. 1) improved computational or analytical methods for analyzing distribution of metals among components of the sediments; 2) improved computational methods for assessing the influences of metal form in sediments on sediment-water metal exchange; and 3) a better understanding of the processes controlling bioaccumulation of metals from solution and food by metazoan species directly exposed to the sediments. Such capabilities would allow mechanistic explanations essential to the development of practical tools sought for determining sediment quality criteria for metals.

Hydrobiologia↗
USGS2022

Special section overview: Effects of ecosystem change on North American percid populations.

Walleye Sander vitreus , Sauger S. canadensis , and Yellow Perch Perca flavescens (referred to as percids herein) are collectively among the most culturally and ecologically important fish species in North America. As ecosystems change in response to environmental drivers, such as climate change, nutrient loading, and invasive species, there is a need to understand how percid populations respond to these changes. To address this need, a symposium was held during the 81st Annual Midwest Fish and Wildlife Conference to bring fishery scientists and managers together to describe and discuss percid population responses to ecosystem change. Prevailing symposium themes included the challenge of identifying mechanisms responsible for population-level changes, developing strategies to adaptively manage for resilient fisheries, and consideration of scale, context, and methods when interpreting variable results. Given the uncertainty of how ecosystem changes affect percid populations, participants emphasized the importance of communicating uncertainties to stakeholders, implementing data-driven management strategies, setting realistic goals, and revising management actions in an adaptive framework. There was universal agreement on both the challenge and necessity of facilitating constructive engagement among stakeholders in cooperative decision making. Symposium participants identified knowledge gaps and discussed future efforts to build on our current understanding of percid populations, including continuation of long-term monitoring, improved standardization of evaluation metrics, implementing adaptive management experiments to identify causal relationships, development of more robust analytical methods, use of historical data sources, and refining techniques to realistically convey management options to stakeholders.

North American Journal of Fisheries Management↗
USGS2023

Improving ecological data science with workflow management software

Pressing environmental research questions demand the integration of increasingly diverse and large-scale ecological datasets as well as complex analytical methods, which require specialized tools and resources. Computational training for ecological and evolutionary sciences has become more abundant and accessible over the past decade, but tool development has outpaced the availability of specialized training. Most training for scripted analyses focuses on individual analysis steps in one script rather than creating a scripted pipeline, where modular functions comprise an ecosystem of interdependent steps. Although current computational training creates an excellent starting place, linear styles of scripting can risk becoming labor- and time-intensive and less reproducible by often requiring manual execution. Pipelines, however, can be easily automated or tracked by software to increase efficiency and reduce potential errors. Ecology and evolution would benefit from techniques that reduce these risks by managing analytical pipelines in a modular, readily parallelizable format with clear documentation of dependencies. Workflow management software (WMS) can aid in the reproducibility, intelligibility and computational efficiency of complex pipelines. To date, WMS adoption in ecology and evolutionary research has been slow. We discuss the benefits and challenges of implementing WMS and illustrate its use through a case study with the targets r package to further highlight WMS benefits through workflow automation, dependency tracking and improved clarity for reviewers. Although WMS requires familiarity with function-oriented programming and careful planning for more advanced applications and pipeline sharing, investment in training will enable access to the benefits of WMS and impart transferable computing skills that can facilitate ecological and evolutionary data science at large scales.

Methods in Ecology and Evolution↗
USGS2017

Methods used to characterize the chemical composition and biological activity of environmental waters throughout the United States, 2012-14

A vast array of chemical compounds are in wide commercial use in the United States, and the potential ecological and human-health effect of exposure to chemical mixtures has been identified as a high priority in environment health science. Awareness of the potential effects of low-level chemical exposures is rising. The U.S. Geological Survey, in cooperation with the U.S. Environmental Protection Agency, conducted a study in which samples were collected from 38 streams in 25 States to provide an overview of contaminants found in stream water across the Nation. Additionally, biological screening assays were used to help determine any potential ecological and human-health effects of these chemical mixtures and to prioritize target chemicals for future toxicological studies. This report describes the site locations and the sampling and analytical methods and quality-assurance procedures used in the study.

Open-File Report↗
USGS2010

Understanding beach health throughout the Great Lakes-Entering a new era of investigations

For over a decade, the U.S. Geological Survey (USGS) has been a leader in the science of beach health. The overall mission of this work is to provide science-based information and methods that will allow beach managers to more accurately make beach closure and advisory decisions, understand the sources and physical processes affecting beach contaminants, and understand how science-based information can be used to mitigate and restore beaches and protect the public. The work consists of four science elements-real-time assessments; pathogens and microbial source tracking; coastal processes; and data analysis, interpretation, and communication - which are described in this fact sheet. Some of the key questions for USGS beach research are the following: Are there better ways to inform the public whether they can use a beach without risking their health? How do new rapid analytical methods compare to traditional methods for determining concentrations of fecal-indicator bacteria at beaches? Are pathogens present at beaches and, if so, how do they get to the beach, and what is their source? How do sand movement and wave action on the beach affect fecal-indicator-bacteria and pathogen concentrations in the lake water? What are the best indicators of pathogenic microorganisms? With so many potential sources of fecal contamination at a beach, what methods can be used to distinguish the contributions from humans? What characteristics of beaches contribute most to influencing bacterial indicator and pathogen concentrations in beach sands and groundwater?

Fact Sheet↗
USGS2025

Effects of nonmotorized recreation on ungulates in the western United States—A science synthesis to inform National Environmental Policy Act analyses

The U.S. Geological Survey is working with Federal land management agencies to develop a series of science syntheses to support National Environmental Policy Act (NEPA) analyses. This report synthesizes science information about the potential effects of nonmotorized recreation on ungulates in the western United States. We conducted a structured literature search to find published science, data, and analysis methods about the characteristics of nonmotorized recreation, ungulate exposure and response to nonmotorized recreation, and approaches to mitigate negative effects of nonmotorized recreation on ungulates. The sections of the report align with standard elements of the NEPA analysis process. We found that timing, intensity, duration, and spatial distribution of nonmotorized recreation are important factors to understand when assessing effects of recreation on ungulates. Several aspects of ungulate biology, which vary by species, population, and individual, affect ungulate susceptibility to effects from recreation, including diet, migration and movement, and seasonal biology. Techniques for assessing effects include basic spatial analyses based on buffers around trails and recreation sites and more technical analytical methods based on displacement or avoidance of recreation sites. Options for mitigating negative effects of nonmotorized recreation on ungulates include timing and type-of-use restrictions, recreator education, and project design features to avoid human-ungulate conflicts. Public land managers can use this report by incorporating it by reference in NEPA analyses or as a general reference to find literature or identify gaps in the literature about the effects of noise from nonmotorized recreation on ungulates.

Scientific Investigations Report↗
USGS2025

The importance of method selection when estimating diet composition with quantitative fatty acid signature analysis

Quantitative fatty acid signature analysis (QFASA) is a common method of estimating the composition of prey species in the diets of consumers from polar and temperate ecosystems in which lipids are an important source of energy. A key characteristic of QFASA is that the large number of fatty acids that typically comprise lipids permits the dietary contributions of a correspondingly large number of prey types to be estimated. Several modifications to the original QFASA methods have been suggested in the literature and a significant extension of the original model published in 2017 allows simultaneous estimation of both diet proportions and calibration coefficients, which are metabolic constants in the model whose values must otherwise be estimated in independent feeding experiments. However, comparisons of diet estimates obtained using different estimation options have been limited. QFASA has been used to estimate the diet composition of several polar bear ( Ursus maritimus ) subpopulations, including the Southern Beaufort Sea (SBS) subpopulation. Prior QFASA estimates of SBS polar bear diet composition have most often been obtained using variations of the original QFASA model. We investigated the influence of variations in QFASA analytical methods on diet estimates by re-estimating the diet composition of polar bears from the Alaska portion of the SBS using three different methods and found that differences among the three sets of estimates were substantial. Our results illustrate how important the careful and deliberate selection of QFASA methods can be and we provide some guidance on techniques one might use to evaluate options.

PLoS ONE↗
USGS2010

Long-term frozen storage of stream water samples for dissolved orthophosphate, nitrate plus nitrite, and ammonia analysis

Many researchers have used freezing as an effective, short-term, water sample preservation method for subsequent nutrient analysis. In this study, filtered samples held at −16±2°C for 4–8 years were reanalyzed for orthophosphate, nitrate plus nitrite, and ammonia. Orthophosphate and ammonia concentrations decreased by 0.2 μg P/L and 5 μg N/L, respectively, at mean concentrations of 69.4 μg P/L and 246 μg N/L. Nitrate plus nitrite increased by 1.1 μg N/L at a mean concentration of 139.1 μg N/L. An anaerobic well sample proved to be unsuitable for freezing because it lost significant amounts of orthophosphate during the freezing process. None of the differences observed over long periods of frozen storage were more than twice the estimated standard deviation of the analytical methods used in the study. The small changes observed demonstrate the effectiveness of frozen storage as a means of nutrient preservation in water samples that are unaffected by the freezing process itself.

Water Resources Research↗
USGS1948

Application of Brianchon's theorem to construction of geologic profiles

Brianchon's theorem states that the three diagonals joining opposite vertices of a hexagon circumscribed about a conic are concurrent. A corollary of this theorem applies to a pentagon so that the points of tangency of an inscribed conic may be located. Any five non-concurrent straight lines in a plane, no three of which are parallel, will ordinarily form some kind of a pentagon; and if considered as tangents to a conic, they will define its shape and position. If these five lines are also normals to the traces of parallel stratigraphic surfaces having a constant strike, the derived conic may be regarded as an evolute, from which a set of involutes can be drawn that will constitute a structural profile. A method is thus afforded for constructing a profile normal to the strike of the rocks, and for measuring stratigraphic thickness, by the utilization of five observations of dip along a suitable linear traverse. Graphical methods are also given for constructing a parabolic evolute from four observations and a circular evolute from three observations. Additional points on the conic evolutes are obtained by the application of Pascal's theorem. A mathematical analysis is presented of the relationship between a conic and five of its tangents; and the conic evolute, rather than its involutes, is recommended as a satisfactory record of the structure of a parallel fold. To obtain the equation of this evolute, the equations of the five tangents are first derived, using trilinear coordinates. Thereafter the tangential and trilinear equations of the general conic are deduced. Criteria are given for classifying the conic as a hyperbola, ellipse, parabola, or circle. These graphical and analytical methods are adaptations of the general method of evolute and involutes. They are offered, not as substitutes for the general method, but as quicker, though somewhat less accurate, means of obtaining similar results, where structural conditions justify their use. © 1948, The Geological Society of America, Inc.

Geological Society of America Bulletin↗
USGS2007

Methods and data used to investigate polonium-210 as a source of excess gross-alpha radioactivity in ground water, Churchill County, Nevada

Ground water is the major source of drinking water in the Carson River Basin, California and Nevada. Previous studies have shown that uranium and gross-alpha radioactivities in ground water can be greater than U.S. Environmental Protection Agency Maximum Contaminant Levels, particularly in the Carson Desert, Churchill County, Nevada. Studies also have shown that the primary source of the gross-alpha radioactivity and alpha-emitting radionuclides in ground water is the dissolution of uranium-rich granitic rocks and basin-fill sediments that have their origins in the Sierra Nevada. However, ground water sampled from some wells in the Carson Desert had grossalpha radioactivities greater than could be accounted for by the decay of dissolved uranium. The occurrence of polonium-210 (Po-210) was hypothesized to explain the higher than expected grossalpha radioactivities. This report documents and describes the study design, field and analytical methods, and data used to determine whether Po-210 is the source of excess gross-alpha radioactivity in ground water underlying the Carson Desert in and around Fallon, Nevada. Specifically, this report presents: 1) gross alpha and uranium radioactivities for 100 wells sampled from June to September 2001; and 2) pH, dissolved oxygen, specific conductance, and Po-210 radioactivity for 25 wells sampled in April and June 2007. Results of quality-control samples for the 2007 dataset are also presented.

Open-File Report↗
USGS2026

Three new calcium formate reference materials for δ13C measurements and a redetermination of the R(13C/12C) ratio for VPDB based on proton nuclear magnetic resonance measurements

Rationale Isotope ratio mass spectrometry (IRMS) and proton nuclear magnetic resonance ( 1 H NMR) spectroscopy are independent techniques for determining the isotope ratio R ( 13 C/ 12 C) in organic compounds. However, the lack of suitable reference materials has limited intercalibration of results from these methods. Methods Three high-purity calcium formate isotopic reference materials were developed, each ideal for accurate isotope ratio measurements by both 1 H NMR and isotope-ratio mass spectrometry (IRMS). For each material, R ( 13 C/ 12 C) was determined by 1 H NMR, and the relative isotope abundance ( δ 13 C VPDB-LSVEC ) by IRMS. The combined IRMS and NMR results were used to determine the R ( 13 C/ 12 C) value of Vienna Peedee belemnite (VPDB). Results The combined datasets yield a value for R ( 13 C/ 12 C) of 0.0111050 ± 0.0000047 ( k = 2) for VPDB, which is 6.7‰ lower than the commonly cited value of 0.011180, but similar to other recent determinations. These three calcium formate reference materials exhibit high accuracy across both analytical methods and provide robust tools for instrument calibration, method validation, and inter-laboratory comparison in carbon stable isotope ratio analysis. Conclusions An accurate R ( 13 C/ 12 C) value for VPDB strengthens the link between relative ( δ 13 C VPDB-LSVEC ) values from IRMS and R ( 13 C/ 12 C) isotope ratios from NMR and other techniques. These three calcium formate isotopic reference materials (USGS106, USGS107, and USGS108) are available from the US Geological Survey and provide reference materials for calibration, method validation, and inter-laboratory comparison in carbon isotope ratio analysis.

Rapid Communications in Mass Spectrometry↗
USGS2008

Estimating sediment discharge: Appendix D

Sediment-discharge measurements usually are available on a discrete or periodic basis. However, estimates of sediment transport often are needed for unmeasured periods, such as when daily or annual sediment-discharge values are sought, or when estimates of transport rates for unmeasured or hypothetical flows are required. Selected methods for estimating suspended-sediment, bed-load, bed- material-load, and total-load discharges have been presented in some detail elsewhere in this volume. The purposes of this contribution are to present some limitations and potential pitfalls associated with obtaining and using the requisite data and equations to estimate sediment discharges and to provide guidance for selecting appropriate estimating equations. Records of sediment discharge are derived from data collected with sufficient frequency to obtain reliable estimates for the computational interval and period. Most sediment- discharge records are computed at daily or annual intervals based on periodically collected data, although some partial records represent discrete or seasonal intervals such as those for flood periods. The method used to calculate sediment- discharge records is dependent on the types and frequency of available data. Records for suspended-sediment discharge computed by methods described by Porterfield (1972) are most prevalent, in part because measurement protocols and computational techniques are well established and because suspended sediment composes the bulk of sediment dis- charges for many rivers. Discharge records for bed load, total load, or in some cases bed-material load plus wash load are less common. Reliable estimation of sediment discharges presupposes that the data on which the estimates are based are comparable and reliable. Unfortunately, data describing a selected characteristic of sediment were not necessarily derived—collected, processed, analyzed, or interpreted—in a consistent manner. For example, bed-load data collected with different types of bed-load samplers may not be comparable (Gray et al. 1991; Childers 1999; Edwards and Glysson 1999). The total suspended solids (TSS) analytical method tends to produce concentration data from open-channel flows that are biased low with respect to their paired suspended-sediment concentration values, particularly when sand-size material composes more than about a quarter of the material in suspension. Instantaneous sediment-discharge values based on TSS data may differ from the more reliable product of suspended- sediment concentration values and the same water-discharge data by an order of magnitude (Gray et al. 2000; Bent et al. 2001; Glysson et al. 2000; 2001). An assessment of data comparability and reliability is an important first step in the estimation of sediment discharges. There are two approaches to obtaining values describing sediment loads in streams. One is based on direct measurement of the quantities of interest, and the other on relations developed between hydraulic parameters and sediment- transport potential. In the next sections, the most common techniques for both approaches are briefly addressed.

Book chapter↗
USGS2009

Gas chromatographic–mass spectrometric fragmentation study of phytoestrogens as their trimethylsilyl derivatives: Identification in soy milk and wastewater samples

An analytical method for the identification of eight plant phytoestrogens (biochanin A, coumestrol, daidzein, equol, formononetin, glycitein, genistein and prunetin) in soy products and wastewater samples was developed using gas chromatography coupled with ion trap mass spectrometry (GC/MS–MS). The phytoestrogens were derivatized as their trimethylsilyl ethers with trimethylchlorosilane (TMCS) and N,O- bis (trimethylsilyl)trifluoroacetamide (BSTFA). The phytoestrogens were isolated from all samples with liquid–liquid extraction using ethyl acetate. Daidzein-d 4 and genistein-d 4 labeled standards were used as internal standards before extraction and derivatization. The fragmentation patterns of the phytoestrogens were investigated by isolating and fragmenting the precursor ions in the ion-trap and a typical fragmentation involved the loss of a methyl and a carbonyl group. Two characteristic fragment ions for each analyte were chosen for identification and confirmation. The developed methodology was applied to the identification and confirmation of phytoestrogens in soy milk, in wastewater effluent from a soy-milk processing plant, and in wastewater (influent and effluent) from a treatment plant. Detected concentrations of genistein ranged from 50,000 μg/L and 2000 μg/L in soy milk and in wastewater from a soy-plant, respectively, to 20 μg/L and <1 μg/L for influent and effluent from a wastewater treatment plant, respectively.

Journal of Chromatography A↗
USGS2022

Quantitative method development to determine feed consumption using a dye

Although there are many methods to determine ingestion and absorption of aquafeeds, none exist that are simple, cost-effective, and quantitative and that can mark fish with a long-lasting, visible indicator. In addition to aquafeed development, selective baits are needed that can be used for aquatic invasive species removal efforts, including for Grass Carp Ctenopharyngodon idella . Bait incorporated with a pesticide would allow for selective removal of targeted species. A method to quickly assess multiple bait formulations was developed to expediate development for invasive species management. Incorporation of Sudan Black B (SBB) in aquafeeds at concentrations greater than 75 and 120 mg SBB/kg fish resulted in pigmented external soft tissues of Largemouth Bass Micropterus salmoides and Rainbow Trout Oncorhynchus mykiss , respectively, 24 h after consumption. Visual confirmation of consumption was detectable in the gastrointestinal tract at all concentrations tested (≥10 mg SBB/kg) and quantifiable by absorbance measured at 601 nm from extracted SBB in tissues at concentrations less than those required for visual pigmentation. Although SBB was detectable in multiple tissues, fin and mandible tissues yielded the greatest accuracy in estimating consumption from extracted SBB. Compared with other tissues tested, liver tissue accumulated the highest level of SBB but had the greatest variability, while muscle tissue accumulated little detectable SBB. We used the SBB analytical method to compare consumption of six novel baits that were in the initial developmental stages to produce a palatable bait formulation designed to attract Grass Carp for management control. Overwhelming preference of a rapeseed bait formulation was confirmed using SBB as a tracer of consumption in the laboratory; however, use of SBB under natural conditions may be valuable for answering additional questions. Baits incorporated with SBB allowed for the rapid, simultaneous assessment of multiple formulations and could allow for future refinement of management baits, with results available as quickly as 24–72 h after application.

North American Journal of Aquaculture↗
USGS2005

Comparison of mine waste assessment methods at the Rattler mine site, Virginia Canyon, Colorado

In a joint project, the mine waste-piles at the Rattler Mine near Idaho Springs, Colorado, were sampled and analyzed by scientists from the U.S. Geological Survey (USGS) and the Colorado School of Mines (CSM). Separate sample collection, sample leaching, and leachate analyses were performed by both groups and the results were compared. For the study, both groups used the USGS sampling procedure and the USGS Field Leach Test (FLT). The leachates generated from these tests were analyzed for a suite of elements using ICP-AES (CSM) and ICP-MS (USGS). Leachate geochemical fingerprints produced by the two groups for composites collected from the same mine waste showed good agreement. In another set of tests, CSM collected another set of Rattler mine waste composite samples using the USGS sampling procedure. This set of composite samples was leached using the Colorado Division of Minerals and Geology (CDMG) leach test, and a modified Toxicity Characteristic Leaching Procedure (TCLP) leach test. Leachate geochemical fingerprints produced using these tests showed a variation of more than a factor of two from the geochemical fingerprints produced using the USGS FLT leach test. We have concluded that the variation in the results is due to the different parameters of the leaching tests and not due to the sampling or analytical methods.

Colorado↗
USGS2006

Long-term population monitoring: Lessons learned from an endangered passerine in Hawai‘i

Obtaining reliable population estimates is crucial to monitoring endangered species and developing recovery strategies. The palila ( Loxioides bailleui ) is an endangered seed-eating Hawaiian honeycreeper restricted to the subalpine forests of Mauna Kea, a volcano on the island of Hawai‘i, USA. The species is vulnerable to extinction primarily because >90% of the population is concentrated in <30 km 2 of habitat on the western slope of this high, dormant volcano. Annual surveys of the palila population have been conducted for ecological, legal, and other purposes since 1980. Because refinements to sampling protocols and analytical methods have evolved, we examined means of adapting the monitoring program to produce comparable estimates of abundance over the past 25-year period and into the future. We conducted variable circular plot surveys during the nonbreeding season (Jan–Mar) and this used data to obtain estimates of effective detection radius and annual density with Distance 4.0, Release 2. For comparability over the time-series, we excluded from analysis the data from new transects. We partitioned the 25-year data set (1980–1996 and 1997–2004) into 2 separate analyses because, beginning in 1997, observers received more training to reduce their tendency to estimate distances to 5-m intervals. We used geographic strata in the analysis of recent surveys because changes in habitat may have invalidated the density-based strata used previously. By adding observer and year and observer and time of day as co-variables, we improved the model fit to the 2 data sets, respectively. Annual estimates were confounded by changes in sampling methodology and analytical procedures over time. However, the addition of new transects, increased training for observers, and use of exact distance estimates instead of rounding also improved model fit. Habitat characteristics and behavior of palila that potentially influenced detection probability, sampling, analysis, and interpretation were regeneration of trees in response to reduced numbers of introduced browsing mammals, seasonally variable rates of vocalization, non-territoriality, and resource-tracking along an elevation gradient. We believe our adaptive approach to analysis and interpretation of 25 years of annual variable circular plot data could help guide similar long-term monitoring efforts.

Hawai'i↗
USGS2024

Invisible hand of sampling for management: Underlying needs to survey a threatened seabird can bias aggregated data

Aim Surveying for a species of concern ahead of proposed activities that alter its habitat is routine practice in conservation and management. Such surveys may accumulate large datasets that could further elucidate trends in abundance and distribution. However, the as-needed surveying of proposed activities may impart a sample site selection bias on the data if used for another purpose. Management of a threatened, forest-nesting seabird offered an example of this. Here we assessed how resource management planning and survey requirements can bias clearance monitoring survey data collected prior to proposed timber harvests, if those data are used for other purposes. Location Oregon and Washington, USA. Taxon Marbled Murrelet ( Brachyramphus marmoratus ). Methods To assess how timber planning and other factors influenced marbled murrelet survey location selection, we used logistic regression models to examine habitat associations of marbled murrelet survey sites ( n = 9178) encompassing proposed timber harvests, and the survey stations ( n = 38,923) therein, across the murrelet's inland range in Washington and Oregon, USA between 1989 and 2021. We then simulated the effect this selective sampling might have on assessments of occupancy trends. Results Most habitat characteristics considered did influence where surveys were located, with distance to roads often being the strongest predictor of survey location. The strength of selection for each covariate changed over time, such that a habitat characteristic strongly influenced location selection in a year but was less influential in another year. The simulation analysis suggested that the non-random selection of survey sites could profoundly bias assessments of occupancy trends. Conclusions When using these clearance monitoring survey data– or any data–beyond their original purpose, careful consideration should be given to the scope of inference provided and analytical methods used, to ensure that observed trends are the product of biological processes and not biased by sampling artefacts.

Oregon, Washington↗