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Natural inquirer: The citizen science edition

People across the world are helping scientists collect data to answer important research questions. Learn how citizens are getting involved in science, from counting birds and lionfish to reporting earthquakes. All Over the Map, Citizen Science System, Device-ive Science, Invasion of the Song Snatcher, and Lion In Wait.

Book

Water availability for the Western United States--Key scientific challenges

In the Western United States, the availability of water has become a serious concern for many communities and rural areas. Near population centers, surface-water supplies are fully appropriated, and many communities are dependent upon ground water drawn from storage, which is an unsustainable strategy. Water of acceptable quality is increasingly hard to find because local sources are allocated to prior uses, depleted by overpumping, or diminished by drought stress. Some of the inherent characteristics of the West add complexity to the task of securing water supplies. The Western States, including the arid Southwest, have the most rapid population growth in the United States. The climate varies widely in the West, but it is best known for its low precipitation, aridity, and drought. There is evidence that the climate is warming, which will have consequences for Western water supplies, such as increased minimum streamflow and earlier snowmelt events in snow-dominated basins. The potential for departures from average climatic conditions threatens to disrupt society and local to regional economies. The appropriative rights doctrine governs the management of water in most Western States, although some aspects of the riparian doctrine are being incorporated. The 'use it or lose it' provisions of Western water law discourage conservation and make the reallocation of water to instream environmental uses more difficult. The hydrologic sciences have defined the interconnectedness of ground water and surface water, yet these resources are still administered separately by most States. The definition of water availability has been expanded to include sustaining riparian ecosystems and individual endangered species, which are disproportionately represented in the Western States. Federal reserved rights, common in the West because of the large amount of Federal land, exist with quite senior priority dates whether or not water is currently being used. A major challenge for water users in the West is that these reserved rights may supersede other existing users. The minimum amount of water required, however, to sustain native peoples, a riparian system, or an endangered species eventually will need to be known in order to manage the available water supply. Periodic inventory and assessment of the amounts and trends of water available in surface water and ground water are needed to support water management. There is a widespread perception that the amount of available water is diminishing with time. This and other perceptions about water availability should be replaced by objective data and analysis. Some data are presented here for the major Western rivers that show that flows are not decreasing in most streams and rivers in the West. Systematic information is lacking to make broad assessments of ground-water availability, but available data for specific aquifers indicate that these aquifers are being depleted, especially near population centers. The complexity added to the issue of Western water availability by these and other factors gives rise to a significant role of science. Science has played a role in support of Western water development from the beginning, and the role has evolved and changed over time as society's values have changed. In this report, the role of science is discussed in three phases: (1) development and construction, (2) consequences and environmental awareness, and (3) sustainability. The development and construction phase includes some historical accounting of water development in the West and shows how some precedents set in those early days are still applied today.

western United States

Vegetation community monitoring: Forest structure in Klamath Network parks

The Klamath Network, comprising six national park units in northern California and southern Oregon, initiated a vegetation monitoring protocol in 2011 to discern ecologically significant vegetation trends in these parks. The premise of the protocol is that multivariate analyses of species composition data is the most robust means for early detection of vegetation change over time. Here, we present these community metrics based on our sampling efforts from 2011 to 2019. Observations from the first sampling event (2011–2013) were used to establish baseline conditions for the vegetation communities. Observations from subsequent sampling in 2014–2019 were used to identify potential temporal variation in forest structure across habitat types and parks. Park landscapes were categorized into three strata: matrix (low- to mid-elevation upland habitats), riparian (within 10 m of a perennial stream), and high-elevation (above a predefined elevation, park-specific). At the onset of the network’s vegetation monitoring protocol, 241 permanent plots were established at random locations across the three strata. We present summary statistics from three repeated samplings (2011–2019) of each plot, describing variation in forest structure across broad habitat types and parks. Observable differences in forest structure aligned with expected productivity gradients across the parks. Measures of forest structure (vegetation cover, stem density, basal area, tree heights, height to live crown, shrub cover, and surface fuels) were generally higher in mesic sites, compared to sites located in more arid, continental climates. Differences across sampling frames also followed this general pattern of productivity. Matrix and riparian sampling frames had similar ranges of values in most cases, while high elevation sites had relatively lower stem density, basal area, shrub cover, fuels, and recruitment. Notably, we observed a relative lack of change in forest structure over time. This is not surprising given the relatively short (six-year) timespan of observations in each park. The fourth set of Klamath Network surveys (2021–2023) is likely to show substantial changes in vegetation cover and forest structure, particularly for parks that have recently experienced major fires. Continued long-term vegetation monitoring is crucial for understanding ecosystem responses to a rapidly changing world. This report on vegetation composition is the second in a series; upcoming reports will analyze structure and function, aiming to detect spatiotemporal trends.

California, Oregon

U.S. Geological Survey Science for the Wyoming Landscape Conservation Initiative: 2012 annual report

Southwest Wyoming contains abundant energy resources, wildlife, habitat, open spaces, and outdoor recreational opportunities. Although energy exploration and development have been taking place in the region since the late 1800s, the pace of development for fossil fuels and renewable energy increased significantly in the early 2000s. This and the associated urban and exurban development are leading to landscape-level environmental and socioeconomic changes that have the potential to diminish wildlife habitat and other natural resources, and the quality of human lives, in Southwest Wyoming. The potential for negative effects of these changes prompted Federal, State, and local agencies to undertake the Wyoming Landscape Conservation Initiative for Southwest Wyoming.

Wyoming

Looking forward, looking back: Building resilience today community report: St. Michael, AK

The Alaska Climate Adaptation Science Center (AK CASC), in partnership with the Aleutian Pribilof Islands Association (APIA), designed the Looking Forward, Looking Back: Building Resilience Today (hereafter ‘BRT’) project as a series of trainings and workshops with tribal community leadership and members. The overarching goal of the project was to collaboratively develop the Indigenous knowledge and western science knowledge for adaptation planning. We worked with five community teams consisting of up to four leaders from communities that chose to participate in the project: Iliamna, Kotlik, Kwigillingok, Quinhagak, and St. Michael. Community teams were developed through the application process and the project duration. Community teams were encouraged to have involvement from multiple governing bodies within the community that could include the Tribal Council, city government, and village corpora- tion. The project title, with its references to the future (Looking Forward), past (Looking Back), and present (Building Resilience Today), refers to the idea that adaptation planning relies on all three perspectives. Equally important, how- ever, is the dialogue to exchange past and present information, context, and what we expect in the future. According- ly, two training sessions held at the International Arctic Research Center in Fairbanks, Alaska at the beginning and near the end of the project were developed to provide community team interaction with each other and with university and federal science partners. The project team also traveled to the partner communities and held a series of onsite events with community members to document locally-relevant information and share climate science tailored to the needs and conditions of each community. This report represents the community information shared during those onsite events. The Meeting Announcement (page 5) shows the date and description of the outreach events. The purpose of these events was to: 1) facilitate mapping of a Traditional Use Area to refine an area for climate pro- jections; 2) construct current and past seasonal Subsistence Calendars to identify important species and times of the year; 3) document Indigenous and local knowledge from current community members about environmental changes they have observed over their lifetimes; and 4) assist with documenting what the community perceived to be climate-related issues through photos and interviews. The agenda of the visits was co-produced with the commu- nity team. In each community, the community team and the project team co-hosted an open-to-the-public meeting and met with various groups. The community team advertised the meetings by posting community fliers, making announcements on the community radio, and reaching out to individuals that would contribute to the engagement discussions. Each community meeting focused on activities to develop seasonal Subsistence Calendars, map Tradition- al Use Areas, and document observed environmental changes. Community members spent time at stations dedicated to each of these activities working with project team members. The project team also met with various groups of indi- viduals that included village corporation, tribal council, and city representatives where additional information about observed environmental changes was gathered. This community report presents some of the information developed in these activities.

Alaska

Marine predator surveys in Glacier Bay National Park and Preserve

Since 1999, vessel based surveys to estimate species composition, distribution and relative abundance of marine birds and mammals have been conducted along coastal and pelagic (offshore) transects in Glacier Bay, Alaska. Surveys have been conducted during winter (November-March) and summer (June). This annual report presents the results of those surveys conducted in March and June of 2001. Following completion of surveys in 2002 we will provide a final report of the results of all surveys conducted between 1999 and 2002. Glacier Bay supports diverse and abundant assemblages of marine birds and mammals. In 2001 we identified 58 species of bird, 7 species of marine mammal, and 6 species of terrestrial mammal on transects sampled during winter and summer. Of course all species are not equally abundant. Among all taxa, in both seasons, sea ducks were the numerically dominant group. In their roles as consumers and because of their generally large size, marine mammals are also likely important in the consumption of energy produced in the Glacier Bay ecosystem. Most common and abundant marine birds and mammals can be placed in either a fish based (e.g. alcids and pinnipeds), or a benthic invertebrate (e.g. sea ducks and sea otters) based food web. Distinct differences in the species composition and abundance of marine birds were observed between winter and summer surveys. Winter marine bird assemblages were dominated numerically (> 11,000; 65% of all birds) by a relatively few species of sea ducks (scoters, goldeneye, Bufflehead, Harlequin and Long-tailed ducks). The sea ducks were distributed almost exclusively along near shore habitats. The prevalence of sea ducks during the March surveys indicates the importance of Glacier Bay as a wintering area for this poorly understood group of animals that occupy a high trophic position in a principally benthic invertebrate (mussel and clam) food web. Marine mammal assemblages were generally consistent between seasons, although Humpback and Killer whales were not observed in winter 2001. Summer marine bird assemblages remained numerically dominated by sea ducks, but species composition shifted between the goldeneye whose density was 44/m 2 in winter to < 0.2/m 2 in summer, to scoters, whose density was 29/m 2 in winter to > 60/m 2 in summer. Large increases in Black-legged kittiwake, murrelet (Marbled and Kittlitz’s) and Common merganser densities were detected during summer surveys. Seasonal differences in abundance of species likely reflected differences in life history attributes (e.g. reproductive biology, foraging ecology) among species. Because of differences observed in species composition between the winter and summer, it is apparent that a single annual survey cannot accurately describe the populations of marine birds and mammals that occur in Glacier Bay. Preliminary analysis further suggests that interpretations of data resulting from this type of survey may depend to a large extent on the individual species. Because species exhibit differences in behavior, morphology, coloration, and distribution, accuracy and precision of abundance estimates likely vary among species. Confidence in survey results should be evaluated in consideration of life history and detection probabilities at the species level. However, survey results likely provide reasonable estimates of species composition and relative abundance, as well as accurate abundance estimates for those species whose detection closely approximates one.

Alaska

Estimating the effects of wetland conservation practices in croplands: Approaches for modeling in CEAP–Cropland Assessment

Quantifying the current and potential benefits of conservation practices can be a valuable tool for encouraging greater practice adoption on agricultural lands. A goal of the CEAP-Cropland Assessment is to estimate the environmental effects of conservation practices that reduce losses (exports) of soil, nutrients, and pesticides from farmlands to streams and rivers. The assessment approach combines empirical data on reported cropland practices with simulation modeling that compares field-level exports for scenarios “with practices” and “without practices.” Conserved, restored, and created wetlands collectively represent conservation practices that can influence sediment and nutrient exports from croplands. However, modeling the role of wetlands within croplands presents some challenges, including the potential for negative impacts of sediment and nutrient inputs on wetland functions. This Science Note outlines some preliminary solutions for incorporating wetlands and wetland practices into the CEAP-Cropland modeling framework. First, modeling the effects of wetland practices requires identifying wetland hydrogeomorphic type and accounting for the condition of both the wetland and an adjacent upland zone. Second, modeling is facilitated by classifying wetland-related practices into two functional categories (wetland and upland buffer). Third, simulating practice effects requires alternative field configurations to account for hydrological differences among wetland types. These ideas are illustrated for two contrasting wetland types (riparian and depressional).

CEAP-Wetlands Science Note

Preliminary testing of flow-ecology hypotheses developed for the GCP LCC region

The Ecological Limits of Hydrological Alteration (ELOHA) framework calls for the development of flow-ecology hypotheses to support protection of the flow regime from ecologically harmful alteration due to human activities. As part of a larger instream flow project for the Gulf Coast Prairie Landscape Conservation Cooperative (GCP LCC), regional flow-ecology hypotheses were developed for fish, mussels, birds, and riparian vegetation (Davis and Brewer 20141 ). The objective of this study was to assess the usefulness of existing ecological and hydrological data to test these hypotheses or others that may be developed in the future. Several databases related to biological collections and hydrologic data from Oklahoma, Texas, and Louisiana were compiled. State fish-community data from Oklahoma and Louisiana were summarized and paired with existing USGS gage data having at least a 40-year period of record that could be separated into reference and current conditions for comparison. The objective of this study was not to conduct exhaustive analyses of these data, the hypotheses, or analyses interpretation, but rather to use these data to determine if existing data were adequate to statistically test the regional flow-ecology hypotheses. The regional flow-ecology hypotheses were developed for the GCP LCC by a committee chaired by Shannon Brewer and Mary Davis (Davis and Brewer 2014). Existing data were useful for informing the hypotheses and suggest support for some hypotheses, but also highlight the need for additional testing and development as some results contradicted hypotheses. Results presented here suggest existing data are adequate to support some flow-ecology hypotheses; however, lack of sampling effort reported with the fish collections and the need for ecoregion-specific analyses suggest more data would be beneficial to analyses in some ecoregions. Additional fish sampling data from Texas and Louisiana will be available for future analyses and may ameliorate some of the data concerns and improve hypothesis interpretation. If the regional hydrologic model currently under development by the U.S. Geological Survey for the South-Central Climate Science Center is improved to produce daily hydrographs, it will enable use of fish data at ungaged locations. In future efforts, exhaustive analyses using these data, in addition to the development of more complex multivariate hypotheses, would be beneficial to understanding data gaps, particularly as relevant to species of conservation concern.

Cooperator Science Series

Biochar for soil fertility and natural carbon sequestration

Biochar is charcoal (similar to chars generated by forest fires) that is made for incorporation into soils to increase soil fertility while providing natural carbon sequestration. The incorporation of biochar into soils can preserve and enrich soils and also slow the rate at which climate change is affecting our planet. Studies on biochar, such as those cited by this report, are applicable to both fire science and soil science.

Fact Sheet

Documentation of methods and inventory of irrigation information collected for the 2015 U.S. Geological Survey estimated use of water in the United States

The U.S. Geological Survey (USGS) National Water-Use Science Project strives to report water-use estimates using the best available information for the period of the estimates. The information available on water used for irrigation activities varies from State to State and in some areas from county to county within a State, which results in many information sources and methods being used to estimate water withdrawals and consumption for the Nation. The variety of estimation methods makes it difficult to compare information across States and makes it difficult to understand how different methods or data sources bias irrigation water-use estimates and trends over time. The sources of information and methods used by USGS Water Science Centers to estimate irrigation water use (the number of irrigated acres by irrigation system type, withdrawal values by water source type, and consumed-water values) for 2015 are compiled and described herein to assist with interpreting the water-use estimates. State-level summaries of information sources and methods are compiled in appendix 1, and a dataset of calendar-year, county-level estimates of actual evapotranspiration for the conterminous United States and Hawaii is provided in an associated USGS data release.

Scientific Investigations Report

System characterization report on Resourcesat-2A Advanced Wide Field Sensor

Executive Summary This report documents the system characterization of the Indian Space Research Organisation Resourcesat-2A Advanced Wide Field Sensor (AWiFS) and is part of a series of system characterization reports produced by the U.S. Geological Survey Earth Resources Observation and Science Cal/Val Center of Excellence. These reports describe the methodology and procedures used for characterization, present technical and operational information about the specific sensing system being evaluated, and provide a summary of test measurements, data retention practices, data analysis results, and conclusions. Resourcesat-2A was launched in 2016 on the Polar Satellite Launch Vehicle-C36; it is identical to Resourcesat-2, and together, they decrease imaging revisit time from 5 days to 2–3 days, providing data continuity and improved temporal resolution. Resourcesat-2 and -2A carry the AWiFS, Linear Imaging Self Scanning-3, and Linear Imaging Self Scanning-4 medium-resolution imaging sensors, continuing the legacy of the Indian Space Research Organisation’s Indian Remote Sensing-1C/1D/P3 satellite programs. More information about Indian Space Research Organisation satellites and sensors is available through the Joint Agency Commercial Imagery Evaluation Earth Observing Satellites Online Compendium and from the Indian Space Research Organisation at https://www.isro.gov.in/ . The Earth Resources Observation and Science Cal/Val Center of Excellence system characterization team assessed the geometric, radiometric, and spatial performance of the Resourcesat-2A AWiFS sensor. Geometric performance is divided into the interior geometric performance of band-to-band registration and the exterior geometric performance of geolocation accuracy. The interior geometric performance had offsets in the range of −1.10 meters (m; −0.020 pixel) to 3.67 m (0.066 pixel) in easting and −5.68 m (−0.101 pixel) to 10.38 m (0.185 pixel) in northing with root mean square error values from 5.60 m (0.100 pixel) to 11.31 m (0.202 pixel) in easting and from 3.00 m (0.054 pixel) to 13.52 m (0.241 pixel) in northing. The exterior geometric performance had mean offsets of −25.29 m in easting and 16.22 m northing with root mean square error values of 26.07 m in easting and 17.60 m in northing compared to the Landsat 8 Operational Land Imager sensor. The radiometric performance had offsets from −0.002 to 0.029 and slopes from 0.733 to 1.012. Spatial performance was in the range of 1.354 to 1.639 pixels for full width at half maximum with a modulation transfer function at a Nyquist frequency in the range of 0.108 to 0.174.

Open-File Report

System characterization report on Resourcesat-2A Linear Imaging Self Scanning-4 sensor

Executive Summary This report documents the system characterization of the Indian Space Research Organisation Resourcesat-2A Linear Imaging Self Scanning-4 (LISS–4) sensor. It is part of a series of system characterization reports produced by the U.S. Geological Survey Earth Resources Observation and Science Cal/Val Center of Excellence. These reports describe the methodology and procedures used for characterization, present technical and operational information about the specific sensing system being evaluated, and provide a summary of test measurements, data retention practices, data analysis results, and conclusions. Resourcesat-2A was launched in 2016 on the Polar Satellite Launch Vehicle-C36; it is identical to Resourcesat-2, and together, they decrease imaging revisit time from 5 days to 2–3 days, providing data continuity and improved temporal resolution. Resouresat-2 and 2A carry the Advanced Wide Field Sensor, Linear Imaging Self Scanning-3, and LISS–4 medium-resolution imaging sensors, continuing the legacy of the Indian Space Research Organisation’s Indian Remote Sensing-1C/1D/P3 satellite programs. More information about the Indian Space Research Organisation’s satellites and sensors is available through the Joint Agency Commercial Imagery Evaluation Earth Observing Satellites Online Compendium at https://calval.cr.usgs.gov/apps/compendium/ and from the manufacturer at https://www.isro.gov.in/ . The Earth Resources Observation and Science Cal/Val Center of Excellence system characterization team assessed the geometric, radiometric, and spatial performances of the Resourcesat-2A LISS–4 sensor. Geometric performance is divided into the interior geometric performance of band-to-band registration and the exterior geometric performance of geolocation accuracy. The interior geometric performance had mean offsets in the range of −0.118 to 0.024 pixel in easting and −0.053 to 0.022 pixel in northing with root mean square error values from 0.067 to 0.230 pixel in easting and from 0.087 to 0.2 pixel in northing. The exterior geometric performance had offsets in the range of 2.55 to 7.85 meters (m) in easting and −6.15 to 11.15 m in northing with root mean square error values in the range of 2.6 to 8.2 m in easting and 6.35 to 11.8 m in northing compared to the U.S. Department of Agriculture National Agriculture Imagery Program and WorldView-3 orthoimages. The measured radiometric performance had offsets from 0.003 to 0.024 and slopes from 0.736 to 0.952, and spatial performance was in the range of 1.633 to 1.903 pixels for the full width at half maximum with a modulation transfer function at a Nyquist frequency in the range of 0.0529 to 0.0952.

Open-File Report

Quality assessment of past spawning mark estimations from a long-term survey in the Connecticut River watershed

The calcified structures of fishes provide insight into their periodic growth rates and can be combined with other biological variables to identify metrics such as size or age at maturity and mortality rates. Collecting this information on growth and life history can help evaluate the success of conservation efforts and inform future management decisions for a species in need. However, before these life history data can be applied to larger stock assessments that direct management decisions, confidence in the validity of the data needs to be reported through metrics of accuracy and precision. For this report, we assessed the bias and precision of paired reader estimations of spawning marks on scales of Blueback herring (Alosa aestivalis) collected in the U.S. Fish & Wildlife (USFWS) Annual Adult River Herring Stock Assessment for the lower Connecticut River basin. The paired reads on scales from a total of 8,698 fish over the ten years of the long term monitoring program were evaluated for the annual presence of systematic bias and precision using a combination of qualitative (i.e., frequency tables) and quantitative (i.e., Evan’s and Hoenig’s Test of Symmetry, and Coefficient of Variation (CV) calculations) analyses. While seven out of the ten survey years had systematic bias detected by the tests of symmetry, only three years (2013, 2016, 2018) had imprecision values >10% CV threshold. Data were further categorized into specific age classes within survey years to increase our resolution on where bias and imprecision was most prevalent. While the ability for accurate bias detection was limited by sufficient sample sizes (>25 fish), average imprecision values increased with age, and median age classes (4 through 6) commonly had bias detected. However, the removal of insufficient age classes prior to calculating average annual CV did not significantly change the initial average. Lastly, 2023, which was the first year to implement a standardized training procedure prior to production estimating, had the highest precision for both the annual average and specific age classes compared to all prior survey years. This standardized training procedure will continue to be used by USFWS for the lower Connecticut River tributaries, and can be modified for other river systems. Overall, this report’s results highlight the importance of assessing precision and encourage the standardization of spawning mark identification quality control and assurance for future studies. With more quality assessments and baseline information on precision and bias, there can be more beneficial discussion on defining thresholds and how to implement spawning history variability into catch curve analyses.

Connecticut, Massachusetts

System characterization report on Tanager

Executive Summary This report addresses the system characterization of the Tanager satellite hyperspectral sensor created by Planet Labs PBC and is part of a series of system characterization reports produced and delivered by the U.S. Geological Survey Earth Resources Observation and Science Cal/Val Center of Excellence. These reports present and detail the methodology and procedures for characterization; present technical and operational information about the Tanager hyperspectral sensor; and provide a summary of test measurements, data retention practices, data analysis results, and conclusions. This report summarizes the sensor performance of the Tanager based on the U.S. Geological Survey Earth Resources Observation and Science Cal/Val Center of Excellence system characterization process. In summary, we determined that the Tanager exhibits a band- to- band geometric error ranging from −0.074 to 0.097 pixel. Compared to the Landsat Operational Land Imager, geometric offsets ranged from −5.980 meters (−0.20 pixel) to 11.348 meters (0.40 pixel). Radiometric comparisons showed offsets between −0.004 and 0.056 with slopes from 0.830 to 1.066. Spectral shifts are found between 0.65 and 0.75 nanometers. Finally, spatial performance evaluation yielded a point spread function full width at half maximum of 1.27 to 1.75 pixels, a relative edge response of 0.802 to 0.651, and a modulation transfer function at Nyquist of 0.488 to 0.253.

Open-File Report

Undergraduate research projects help promote diversity in the geosciences

A workforce that draws from all segments of society and mirrors the ethnic, racial, and gender diversity of the United States population is important. The geosciences (geology, hydrology, geospatial sciences, environmental sciences) continue to lag far behind other science, technology, engineering and mathematical (STEM) disciplines in recruiting and retaining minorities (Valsco and Valsco, 2010). A report published by the National Science Foundation in 2015, “Women, Minorities, and Persons with Disabilities in Science and Engineering” states that from 2002 to 2012, less than 2% of the geoscience degrees were awarded to African-American students. Data also show that as of 2012, approximately 30% of African-American Ph.D. graduates obtained a bachelor’s degree from a Historic Black College or University (HBCU), indicating that HBCUs are a great source of diverse students for the geosciences. This paper reviews how an informal partnership between Tennessee State University (a HBCU), the U.S. Geological Survey, and Mammoth Cave National Park engaged students in scientific research and increased the number of students pursuing employment or graduate degrees in the geosciences.

Conference Paper

Maintaining frog monitoring and population on the Las Cienegas National Conservation Area

This report summarizes and analyzes the data collected on the Chiricahua Leopard Frog (CLF) populations on the Las Ciénegas National Conservation Area (LCNCA) in southeast Arizona, U.S.A., during the reporting period covered under this funding agreement from September 2020 through October 2024 and place this analysis in historical context of the previous monitoring efforts. The major findings of this report are: 1. We conducted a total of 465 surveys across 46 sites on the LCNCA and the associated Elgin Buffer Zone during September 2020 through October 2024. We conducted 323 surveys across the 19 core CLF monitoring sites on the LCNCA and 112 surveys across 13 core buffer zone sites. 2. We detected CLF at each of our 19 core LCNCA monitoring sites during at least one year in which we conducted surveys across the year’s entire field season (i.e., 2021-2024). We detected CLF reproduction in each of these four years at four sites and suspected reproduction to have occurred during all four years at a fifth site. We did not detect CLF reproduction at seven of these 19 core monitoring sites during the report period. We assisted with six CLF translocations to sites on the LCNA during the report period. 3. Site occupancy rates across the LCNCA during the report period remain consistent with those reported during previous years and continue to indicate the presence of a stable CLF metapopulation within the LCNCA. 4. Monitoring efforts in the Elgin Buffer Zone continue to be successful at preventing reinvasion by invasive American bullfrogs to the LCNCA. We detected and removed bullfrogs from three sites in the Elgin Buffer Zone and no bullfrogs were detected during 2024. The LCNCA continues to remain bullfrog free since 2014.

Arizona