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An evaluation of blood plasma for monitoring DDE in birds of prey

Laboratory and field studies show that DDE residues in blood plasma are highly correlated with DDE in the brain, the rate of DDE exposure and the amount of DDE in eggs of free-living birds of prey. A blood sampling approach is proposed to supplement existing environmental contaminant methods available for birds of prey. Residues (ΣDDT) in plasma provide some direct information; however, a method is proposed to adjust blood plasma residues from four species of birds of prey to the estimated residues in eggs for the purpose of residue interpretation. The blood plasma-egg relationship appears to be species-independent in the four raptors studied. Two predicting equations are presented for estimating egg residues, one for plasma samples collected prior to, or within a few days of, egg laying, and another for the post-laying period. Limitations and advantages of the blood plasma monitoring approach are discussed. The approach may be particularly suitable for endangered species research.

Environmental Pollution (Series A)

Raptor selection of captive reared and released Galliform birds

Captive rearing and release of birds in the order Galliformes remains a popular management tactic despite low survival rates. We investigated avian predator selection of captive‐reared northern bobwhites ( Colinus virginianus ) as a potential driver of their high mortality. We simulated avian predator hunts on a flushing pair of bobwhites during trials conducted from 6 February 2015 to 14 February 2015 in Texas, USA. When presented a choice of a captive‐reared bobwhite or wild bobwhite, a Harris's hawk ( Parabuteo unicinctus ) pursued a captive‐reared bobwhite in 8 of 10 flight trials. This selective rate, combined with observations from auxiliary research indicate avian predation may be a greater risk for captive‐reared birds than wild birds. The success of captive‐rearing and release efforts may benefit from a better understanding of how to more adequately prepare captive‐reared birds for release.

Texas

Impacts to wildlife of wind energy siting and operation in the United States

Electricity from wind energy is a major contributor to the strategy to reduce greenhouse gas emissions from fossil fuel use and thus reduce the negative impacts of climate change. Wind energy, like all power sources, can have adverse impacts on wildlife. After nearly 25 years of focused research, these impacts are much better understood, although uncertainty remains. In this report, we summarize positive impacts of replacing fossil fuels with wind energy, while describing what we have learned and what remains uncertain about negative ecological impacts of the construction and operation of land-based and offshore wind energy on wildlife and wildlife habitat in the U.S. Finally, we propose research on ways to minimize these impacts. TO SUMMARIZE: 1 Environmental and other benefits of wind energy include near-zero greenhouse gas emissions, reductions of other common air pollutants, and little or no water use associated with producing electricity from wind energy. Various scenarios for meeting U.S. carbon emission reduction goals indicate that a four- to five-fold expansion of land-based wind energy from the current 97 gigawatts (GW) by the year 2050 is needed to minimize temperature increases and reduce the risk of climate change to people and wildlife. 2 Collision fatalities of birds and bats are the most visible and measurable impacts of wind energy production. Current estimates suggest most bird species, especially songbirds, are at low risk of population-level impacts. Raptors as a group appear more vulnerable to collisions. Population-level impacts on migratory tree bats are a concern, and better information on population sizes is needed to evaluate potential impacts to these species. Although recorded fatalities of cave-dwelling bat species are typically low at most wind energy facilities, additional mortality from collisions is a concern given major declines in these species due to white-nose syndrome (WNS). Assessments of regional and cumulative fatality impacts for birds and bats have been hampered by the lack of data from areas with a high proportion of the nation’s installed wind energy capacity. Efforts to expand data accessibility from all regions are underway, and this greater access to data along with improvements in statistical estimators should lead to improved impact assessments. 3 Habitat impacts of wind energy development are difficult to assess. An individual wind energy facility may encompass thousands of acres, but only a small percentage of the landscape within the project area is directly transformed. If a project is sited in previously undisturbed habitat, there is concern for indirect impacts, such as displacement of sensitive species. Studies to date indicate displacement of some species, but the long-term population impacts are unknown. 4 Offshore wind energy development in the U.S. is just beginning. Studies at offshore wind facilities in Europe indicate some bird and marine mammal species are displaced from project areas, but substantial uncertainty exists regarding the individual or population-level impacts of this displacement. Bird and bat collisions with offshore turbines are thought to be less common than at terrestrial facilities, but currently the tools to measure fatalities at offshore wind energy facilities are not available. The wind energy industry, state and federal agencies, conservation groups, academia, and scientific organizations have collaborated for nearly 25 years to conduct the research needed to improve our understanding of risk to wildlife and to avoid and minimize that risk. Efforts to reduce the uncertainty about wildlife risk must keep up withthe pace and scale of the need to reduce carbon emissions. This will require focusing our research priorities and increasing the rate at which we incorporate research results into the development and validation of best practices for siting and operating wind energy facilities.

Issues in Ecology

Effects of hunting on survival of American woodcock in the Northeast

Numbers of American woodcock ( Scolopax minor ) males counted on the annual singing ground survey (SGS) have declined over the last 35 years at an average rate of 2.3% per year in the Eastern Region and 1.8% per year in the Central Region. Although hunting was not thought to be a cause of these declines, mortality caused by hunters can be controlled. Furthermore, there has been no research on effects of hunting mortality on woodcock populations at local and regional levels on the breeding grounds. We used radiotelemetry to determine survival rates and causes of mortality for 913 woodcock captured during fall 1997–2000 on 7 areas in Maine, New Hampshire, Pennsylvania, and Vermont, USA. Three of 7 sites were closed to hunting. For all sites and all years combined, 176 woodcock died, and 130 were censored, of which 39 were censored mortalities. Predation was the major ( n = 134, 76%) cause of mortality. Mammals accounted for 56% of the predation, raptors accounted for 25%, and 19% was attributed to unknown predators. On hunted sites, 36% of the total mortality ( n = 102) was caused by hunting, 63% by predation, and 1 bird starved. Kaplan-Meier survival curves did not differ between hunted and non-hunted sites among years ( P = 0.46). Overall, point estimates of survival did not differ ( P = 0.217) between hunted (SR = 0.636, SE = 0.04) and nonhunted sites (SR = 0.661, SE = 0.08). We modeled hazard rates from hunting and natural mortality events using program MARK. Akaike's Information Criterion supported using a model with common constant hazards from both hunting and natural causes for groups of sites. Groupings of sites for hazard rates from natural causes were not influenced by whether a site was hunted or not. Models detected no effects of woodcock age and sex ( P = 0.52) on survival. Proportional hazards models comparing hunted and nonhunted sites found no effects of age and sex ( P = 0.45), interactions of age, sex, capture weight, and bill length ( P ≥ 0.269). Our data suggest that current hunting regulations are not causing lower survival of woodcock.

Maine, New Hampshire, Pennsylvania, Vermont

Thyroid disruption and oxidative stress in American kestrels following embryonic exposure to the alternative flame retardants, EHTBB and TBPH

Brominated flame retardant chemicals, such as 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EHTBB) (CAS #: 183658–27-7) and bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (TBPH) (CAS #: 26040–51-7), have been detected in avian tissues and eggs from remote regions. Exposure to EHTBB and TBPH has been shown to cause oxidative stress and altered thyroid function in rodents and fish, yet no controlled studies have examined potential adverse effects of exposure in birds. Because flame retardants have been detected in wild raptors, we used American kestrels ( Falco sparverius ) as a model raptor to determine whether in ovo exposure to EHTBB or TBPH affected growth, hatching success, oxidative stress, or thyroid function. We exposed kestrel embryos to nominal concentrations (10, 50, or 100 ng g −1 egg weight) of EHTBB and TBPH via egg-injection on embryonic day 5. Embryonic exposure (~23 d) to EHTBB increased thyroid gland mass, reduced glandular colloid and total thyroxine (T4) in hatchling males and females, whereas deiodinase enzyme activity increased in males but decreased in females. Hatchlings exposed to TBPH in ovo exhibited reduced colloid and increased oxidative stress. Although exposure to EHTBB and TBPH caused several physiological effects (e.g., heart and brain mass), only exposure to 50 ng g −1 EHTBB appeared to reduce hatching success. Our results suggest these flame retardants may be hazardous for predatory birds. Future research should evaluate long-term survival and fitness consequences in birds exposed to these chemicals.

Environment International

Impact of harvest on survival of a heavily hunted game bird population

Context . Despite their economic importance and intensive management, many game bird species, including the northern bobwhite Colinus virginianus , are in decline. Declines may be explained, at least in part, by low survival due perhaps to poor habitat quality, high predation or excessive hunting pressure. Aims . This study sought to estimate and model annual/seasonal survival probabilities, to evaluate factors influencing them and to determine the cause-specific mortality rates for northern bobwhites subject to varying levels of harvest on the Babcock–Webb Wildlife Management Area (BW area), south Florida, USA. Methods . We applied Cox’s proportional hazard models to data collected from 2066 radio-tagged bobwhites during 2002–2008 to test for intrinsic and extrinsic factors affecting survival and the non-parametric cumulative incidence function estimator to estimate cause-specific mortality rates. Key results . Mean annual survival (0.091 ± 0.006) in the BW area was lower than most estimates reported for other bobwhite populations. Annual survival differed between adults (0.111 ± 0.008) and juveniles (0.052 ± 0.008), and varied among years. Survival in winter (October–March; 0.295 ± 0.014) was similar to that in summer (April–September; 0.307 ± 0.013). Density of food strips (i.e. long and narrow food plots) did not influence survival, but hunting effort (number of hunters per day per km 2 ) had a substantial negative impact on survival. In the lightly hunted field trial zone, winter (October–March) survival was significantly higher (0.414 ± 0.035) than in the other more heavily hunted management zones (0.319 ± 0.016). Cause-specific mortality analyses revealed that bobwhite mortality during summer (April–September) was mainly due to raptor (39.7%) and mammalian predation (35.6%), whereas hunting was the primary cause of mortality during winter (47.1%). Conclusions . Our results highlight the potential role of harvest as an important cause of the northern bobwhite population declines in south Florida. High mortality during winter may reduce recruitment of juveniles to the reproductive segment of the population, and ultimately the population growth. Implications . Our results suggest that reduction in hunting pressure may be necessary to reverse the declining population trends in heavily hunted game species in public lands, such as the northern bobwhites in the BW area.

Florida

Saker falcon research and conservation efforts in Mongolia, 1997

This past summer. our small field team followed a 4000 km route through central and eastern Mongolia. Even though there was a population crash underway for picas (Ochotona sp.) and voles (Microtus sp.). we found 38 new saker nests and visited 60 eyries found in previous years. Many of the former eyries were unoccupied. Others were occupied but without young. Productivity was good at eyries with large young. and southeastern Mongolia seemed unaffected by food shortages. The main goal for 1997 was to create new eyries and enlarge. stabilise. or otherwise alter marginal eyries. \Ve created 65 eyries as follows: 8 on wooden powerlines or telephone supports, 8 on metal power line towers, 3 in trees, 3 on boulders, 11 on cliffs, 17 on abandoned buildings, 9 on metal geological survey towers, and 6 on miscellaneous structures. \Ve also enlarged or repaired three establishedeyries and did minor repairs on several others. Lesser accomplishments include what may be the first observation of siblicide for the saker falcon (please contact us immediately if you have other records of sakerchicks attacking or killing their nest mates) and the description of a new saker flight display. We also documented an unusual golden eagle eyrie containing the remains of nearly 30 foxes, several predatory birds, and a number of gazelle. In 1998. we plan to return to Mongolia to see how many of our 'fake eyries' attracted falcons. Our work in 1997 was supported by Mr. Howell. another philanthropist (anonymous) and the Institute of Raptor Studies.

Falco

Re-Occupancy of Breeding Territories by Ferruginous Hawks in Wyoming: Relationships to Environmental and Anthropogenic Factors

Grassland and shrubland birds are declining globally due in part to anthropogenic habitat modification. Because population performance of these species is also influenced by non-anthropogenic factors, it is important to incorporate all relevant ecological drivers into demographic models. We used design-based sampling and occupancy models to test relationships of environmental factors that influence raptor demographics with re-occupancy of breeding territories by ferruginous hawks ( Buteo regalis ) across Wyoming, USA, 2011–2013. We also tested correlations of territory re-occupancy with oil and gas infrastructure—a leading cause of habitat modification throughout the range of this species of conservation concern. Probability of re-occupancy was not related to any covariates we investigated in 2011, had a strong negative relationship with cover of sagebrush ( Artemisia spp.) in 2012, was slightly higher for territories with artificial platforms than other nest substrates in 2013, and had a positive relationship with abundance of ground squirrels ( Urocitellus spp.) that was strong in 2012 and weak in 2013. Associations with roads were weak and varied by year, road-type, and scale: in 2012, re-occupancy probability had a weak positive correlation with density of roads not associated with oil and gas fields at the territory-scale; however, in 2013 re-occupancy had a very weak negative correlation with density of oil and gas field roads near nest sites (≤500 m). Although our results indicate re-occupancy of breeding territories by ferruginous hawks was compatible with densities of anthropogenic infrastructure in our study area, the lack of relationships between oil and gas well density and territory re-occupancy may have occurred because pre-treatment data were unavailable. We used probabilistic sampling at a broad spatial extent, methods to account for imperfect detection, and conducted extensive prey sampling; nonetheless, future research using before-after-control-impact designs is needed to fully assess impacts of oil and gas development on ferruginous hawks.

Wyoming

Increased mortality rates caused by highly pathogenic avian influenza virus in a migratory raptor

Highly pathogenic avian influenza virus (HPAIV) has caused extensive mortalities in wild birds with a disproportionate impact on raptors since 2021. The population-level impact of HPAIV can be informed by telemetry studies that track large samples of initially healthy, wild birds. We leveraged movement data from 71 rough-legged hawks ( Buteo lagopus ) across all major North American migratory bird flyways concurrent with the 2022–2023 HPAIV outbreak and identified a total of 29 mortalities, of which 11 were confirmed, and an additional ~9 were estimated to have been caused by HPAIV. We estimated a 28% HPAIV cause-specific mortality rate among rough-legged hawks during a single year concurrent with the HPAIV outbreak in North America. Additionally, the overall mortality rate during the HPAIV outbreak (47%) was significantly higher than baseline annual mortality rates (3%–17%) suggesting that HPAIV-caused deaths were additive above baseline mortality levels. HPAIV mortalities were concentrated within the Central and Atlantic flyways during prebreeding migration and peaked in April 2022 when large-scale HPAIV mortalities were reported in other wild birds throughout North America. HPAIV exposure was most likely caused by scavenging or preying on infected waterfowl, as rough-legged hawks are known to opportunistically scavenge during the nonbreeding season. We utilized movement data to identify a continental-scale HPAIV cause-specific mortality event in rough-legged hawks that has the potential to exacerbate ongoing population declines. Our study highlights the usefulness of monitoring movement data to pinpoint sources of mortality that can help better understand the drivers of population change, even if studies are focused on other research questions.

Ecology and Evolution

Toxicological responses to sublethal anticoagulant rodenticide exposure in free-flying hawks

An important component of assessing the hazards of anticoagulant rodenticides to non-target wildlife is observations in exposed free-ranging individuals. The objective of this study was to determine whether environmentally realistic, sublethal first-generation anticoagulant rodenticide (FGAR) exposures via prey can result in direct or indirect adverse effects to free-flying raptors. We offered black-tailed prairie dogs ( Cynomys ludovicianus ) that had fed on Rozol® Prairie Dog Bait (Rozol, 0.005% active ingredient chlorophacinone, CPN) to six wild-caught red-tailed hawks (RTHA, Buteo jamaicensis ), and also offered black-tailed prairie dogs that were not exposed to Rozol to another two wild-caught RTHAs for 7 days. On day 6, blood was collected to determine CPN’s effects on blood clotting time. On day 7, seven of the eight RTHAs were fitted with VHF radio telemetry transmitters and the RTHAs were released the following day and were monitored for 33 days. Prothrombin time (PT) and Russell’s viper venom time confirmed that the CPN-exposed RTHAs were exposed to and were adversely affected by CPN. Four of the six CPN-exposed RTHAs exhibited ptiloerection, an indication of thermoregulatory dysfunction due to CPN toxicity, but no signs of intoxication were observed in the reference hawk or the remaining two CPN-exposed RTHAs. Of note is that PT values were associated with ptiloerection duration and frequency; therefore, sublethal CPN exposure can directly or indirectly evoke adverse effects in wild birds. Although our sample sizes were small, this study is a first to relate coagulation times to adverse clinical signs in free-ranging birds.

Colorado