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Biodiversity connections—‘ties that bind’

Connectivity is a foundational concept in ecology and conservation and was the organising theme for the 2022 Annual Meeting of the Southeastern Fishes Council, a professional organisation dedicated to the study and conservation of freshwater fishes native to the southeast region of the United States (US). We introduce a Special Contribution of six papers selected from presentations at that meeting that illustrate perspectives on connections created by fish migration and dispersal, evolved life histories and habitat affinities and interspecific facilitation. Although focused on streams of the southeast US, each of these topics is broadly relevant to freshwater fish conservation, particularly with respect to causes and consequences of migratory fish depletion, population fragmentation and species declines. Many other connections relevant to the ecology and conservation of freshwater fishes remain relatively unexplored but could substantively advance conservation. We highlight the potential that species evolutionary histories, that is connections through time, reconstructed using species distributions and phylogenies may improve predictions of species responses to environmental change. Identifying species interdependencies, including undiscovered interactions that support survival or reproduction, could provide insights into how species losses may cascade as aquatic communities unravel. Finally, efforts to elucidate diverse connections between people and freshwater biodiversity, particularly where fisheries are historic and streams mostly go unnoticed, may prove essential to building public support for conservation measures. A research agenda anchored on ‘biodiversity connections’ has the potential to advance ecological understanding and public engagement, elements essential to conserving freshwater fishes.

Ecology of Freshwater Fish

A comparative analysis of common methods to identify waterbird hotspots

1. Hotspot analysis is a commonly used method in ecology and conservation to identify areas of high biodiversity or conservation concern. However, delineating and mapping hotspots is subjective and various approaches can lead to different conclusions with regard to the classification of particular areas as hotspots, complicating long-term conservation planning and implementation efforts. 2. We present a comparative analysis of recent approaches for identifying waterbird hotspots, with the goal of developing insights about the appropriate use of these methods. We selected four commonly used measures to identify persistent areas of high use: kernel density estimation, Getis-Ord Gi*, hotspot persistence, and hotspots conditional on presence, which represent the range of quantitative hotspot estimation approaches used in waterbird analyses. We applied each of the methods to aerial survey waterbird count data collected in the Great Lakes from 2012-2014 using a 5 km2 grid. For each approach, we identified areas of high use for seven species/species groups and then compared the results across all methods. 3. Our results indicate that formal hotspot analysis frameworks do not always lead to the same conclusions. The kernel density and Getis-Ord Gi* methods yielded the most similar results across all species analyzed. We found that these two models can differ substantially from the hotspot persistence and hotspots conditional on presence estimation approaches, which were not consistently similar to one another. The hotspot persistence approach differed most significantly from the other methods but is the only method to explicitly account for temporal variation. 4. We recommend considering the ecological question and scale of any conservation or management activities prior to designing survey methodologies. Deciding the appropriate definition and scale for analysis is critical for interpretation of hotspot analysis results. Combining methods using an integrative approach, either within a single analysis or post-hoc, could lead to greater consistency in the identification of waterbird hotspots.

Lake Erie, Lake Huron, Lake Michigan

A guide to calculating habitat-quality metrics to inform conservation of highly mobile species

Many metrics exist for quantifying the relative value of habitats and pathways used by highly mobile species. Properly selecting and applying such metrics requires substantial background in mathematics and understanding the relevant management arena. To address this multidimensional challenge, we demonstrate and compare three measurements of habitat quality: graph-, occupancy-, and demographic-based metrics. Each metric provides insights into system dynamics, at the expense of increasing amounts and complexity of data and models. Our descriptions and comparisons of diverse habitat-quality metrics provide means for practitioners to overcome the modeling challenges associated with management or conservation of such highly mobile species. Whereas previous guidance for applying habitat-quality metrics has been scattered in diversified tracks of literature, we have brought this information together into an approachable format including accessible descriptions and a modeling case study for a typical example that conservation professionals can adapt for their own decision contexts and focal populations. Considerations for Resource Managers Management objectives, proposed actions, data availability and quality, and model assumptions are all relevant considerations when applying and interpreting habitat-quality metrics. Graph-based metrics answer questions related to habitat centrality and connectivity, are suitable for populations with any movement pattern, quantify basic spatial and temporal patterns of occupancy and movement, and require the least data. Occupancy-based metrics answer questions about likelihood of persistence or colonization, are suitable for populations that undergo localized extinctions, quantify spatial and temporal patterns of occupancy and movement, and require a moderate amount of data. Demographic-based metrics answer questions about relative or absolute population size, are suitable for populations with any movement pattern, quantify demographic processes and population dynamics, and require the most data. More real-world examples applying occupancy-based, agent-based, and continuous-based metrics to seasonally migratory species are needed to better understand challenges and opportunities for applying these metrics more broadly.

Natural Resource Modeling

Evaluating contributions of recent tracking-based animal movement ecology to conservation management

The use of animal-born sensors for location-based tracking and bio-logging in terrestrial systems has expanded dramatically in the past 10 years. This rapid expansion has generated new data on how animals interact with and respond to variation in their environment, resulting in important ecological, physiological, and evolutionary insights. Although understanding the finer details of animal locations has important management relevance, applied studies are not prominent in the movement ecology literature. This is despite the long history of applied studies of animal movement and the urgent and growing need for evidence-based conservation guidance, especially in the challenging field of human-wildlife interactions. The goal of this review is to evaluate the realized contribution of tracking-based animal movement ecology to solving specific conservation problems, and to identify barriers that may hinder expansion of that contribution. To do this, we (a) briefly review the history and technologies used in animal tracking and bio-logging, (b) use a series of literature searches to evaluate the frequency with which movement ecology studies are designed to solve specific conservation problems, and (c) use this information to identify challenges that may limit the applied relevance of the field of movement ecology, and to propose pathways to expand that applied relevance. Our literature review quantifies the limited extent to which research in the field of movement ecology is designed to solve specific conservation problems, but also the fact that such studies are slowly becoming more prevalent. We discuss how barriers that limit application of these principles are likely due to constraints imposed by the types of data used commonly in the field. Problems of scale mismatch, error compounding, and data paucity all create challenges that are relevant to the field of movement ecology but may be especially pertinent in applied situations. Finding solutions to these problems will create new opportunity for movement ecologists to contribute to conservation science.

Frontiers in Ecology and Evolution

Application of genetics and genomics to wildlife epidemiology

Wildlife diseases can have significant impacts on wildlife conservation and management. Many of the pathogens that affect wildlife also have important implications for domestic animal and human health. However, management interventions to prevent or control wildlife disease are hampered by uncertainties about the complex interactions between pathogens and free-ranging wildlife. We often lack crucial knowledge about host ecology, pathogen characteristics, and host–pathogen dynamics. The purpose of this review is to familiarize wildlife biologists and managers with the application of genetic and genomic methodologies for investigating pathogen and host biology to better understand and manage wildlife diseases. The genesis of this review was a symposium at the 2013 annual Wildlife Society Conference. We reviewed the scientific literature and used our personal experiences to identify studies that illustrate the application of genetic and genomic methods to advance our understanding of wildlife epidemiology, focusing on recent research, new techniques, and innovative approaches. Using examples from a variety of pathogen types and a broad array of vertebrate taxa, we describe how genetics and genomics can provide tools to detect and characterize pathogens, uncover routes of disease transmission and spread, shed light on the ways that disease susceptibility is influenced by both host and pathogen attributes, and elucidate the impacts of disease on wildlife populations. Genetic and increasingly genomic methodologies will continue to contribute important insights into pathogen and host biology that will aid efforts to assess and mitigate the impacts of wildlife diseases on global health and conservation of biodiversity.

Journal of Wildlife Management

The evolutionary history of steelhead ( Oncorhynchus mykiss ) along the US Pacific Coast: Developing a conservation strategy using genetic diversity

Changes in genetic variation across a species range may indicate patterns of population structure resulting from past ecological and demographic events that are otherwise difficult to infer and thus provide insight into evolutionary development. Genetic data is used, drawn from 11 microsatellite loci amplified from anadromous steelhead ( Oncorhynchus mykiss ) sampled throughout its range in the eastern Pacific Ocean, to explore population structure at the southern edge in California. Steelhead populations in this region represent less than 10% of their reported historic abundance and survive in very small populations found in fragmented habitats. Genetic data derived from three independent molecular systems (allozymes, mtDNA, and microsatellites) have shown that the southernmost populations are characterized by a relatively high genetic diversity. Two hypothetical models supporting genetic population substructure such as observed were considered: (1) range expansion with founder-flush effects and subsequent population decline; (2) a second Pleistocene radiation from the Gulf of California. Using genetic and climatic data, a second Pleistocene refugium contributing to a southern ecotone seems more feasible. These data support strong conservation measures based on genetic diversity be developed to ensure the survival of this uniquely diverse gene pool.

ICES Journal of Marine Science

Age and sex specific timing, frequency, and spatial distribution of horseshoe crab spawning in Delaware Bay: Insights from a large-scale radio telemetry array

To study horseshoe crab Limulus polyphemus spawning behavior and migration over a large-spatial extent (>100 km), we arrayed fixed station radio receivers throughout Delaware Bay and deployed radio transmitters and archival tags on adult horseshoe crabs prior to their spawning season. We tagged and released 160 females and 60 males in 2004 and 217 females in 2005. The array covered approximately 140 km of shoreline. Recapture rates were >70% with multi-year recaptures. We categorized adult age by carapace wear. Older females tended to spawn earlier in the season and more frequently than young females, but those tendencies were more apparent in 2004 when spawning overall occurred earlier than in 2005 when spawning was delayed possibly due to decreased water temperatures. Timing of initial spawning within a year was correlated with water temperature. After adjusting for day of first spring tide, the day of first spawning was 4 days earlier for every 1 degree (̊C) rise in mean daily water temperature in May. Seventy nine % of spawning occurred during nighttime high tides. Fifty five % of spawning occurred within 3 d of a spring tide, which was slightly higher than the 47% expected if spawning was uniformly distributed regardless of tidal cycle. Within the same spawning season, males and females were observed spawning or intertidally resting at more than one beach separated by >5 km. Between years, most (77%) did not return to spawn at the same beach. Probability of stranding was strongly age dependent for males and females with older adults experiencing higher stranding rates. Horseshoe crabs staging in the shallow waters east of the channel spawned exclusively along the eastern (NJ) shoreline, but those staging west of the channel spawned throughout the bay. Overall, several insights emerged from the use of radio telemetry, which advances our understanding of horseshoe crab ecology and will be useful in conserving the Delaware Bay horseshoe crab population and habitats.

Delaware, New Jersey

Reducing fertilizer-nitrogen losses from rowcrop landscapes: Insights and implications from a spatially explicit watershed model

We present conceptual and quantitative models that predict changes in fertilizer-derived nitrogen delivery from rowcrop landscapes caused by agricultural conservation efforts implemented to reduce nutrient inputs and transport and increase nutrient retention in the landscape. To evaluate the relative importance of changes in the sources, transport, and sinks of fertilizer-derived nitrogen across a region, we use the spatially explicit SPAtially Referenced Regression On Watershed attributes watershed model to map the distribution, at the small watershed scale within the Upper Mississippi-Ohio River Basin (UMORB), of: (1) fertilizer inputs; (2) nutrient attenuation during delivery of those inputs to the UMORB outlet; and (3) nitrogen export from the UMORB outlet. Comparing these spatial distributions suggests that the amount of fertilizer input and degree of nutrient attenuation are both important in determining the extent of nitrogen export. From a management perspective, this means that agricultural conservation efforts to reduce nitrogen export would benefit by: (1) expanding their focus to include activities that restore and enhance nutrient processing in these highly altered landscapes; and (2) targeting specific types of best management practices to watersheds where they will be most valuable. Doing so successfully may result in a shift in current approaches to conservation planning, outreach, and funding.

Illinois, Indiana, Iowa, Minnesota, Missouri, Ohio

Coping with the cold: An ecological context for the abundance and distribution of rock sandpipers during winter in upper Cook Inlet, Alaska

Shorebirds are conspicuous and abundant at high northern latitudes during spring and summer, but as seasonal conditions deteriorate, few remain during winter. To the best of our knowledge, Cook Inlet, Alaska (60.6˚ N, 151.6˚ W), is the world’s coldest site that regularly supports wintering populations of shorebirds, and it is also the most northerly nonbreeding location for shorebirds in the Pacific Basin. During the winters of 1997–2012, we conducted aerial surveys of upper Cook Inlet to document the spatial and temporal distribution and number of Rock Sandpipers (Calidris ptilocnemis) using the inlet. The average survey total was 8191 ± 6143 SD birds, and the average of each winter season’s highest single-day count was 13 603 ± 4948 SD birds. We detected only Rock Sandpipers during our surveys, essentially all of which were individuals of the nominate subspecies (C. p. ptilocnemis). Survey totals in some winters closely matched the population estimate for this subspecies, demonstrating the region’s importance as a nonbreeding resource to the subspecies. Birds were most often found at only a handful of sites in upper Cook Inlet, but shifted their distribution to more southerly locations in the inlet during periods of extreme cold. Two environmental factors allow Rock Sandpipers to inhabit Cook Inlet during winter: 1) an abundant bivalve (Macoma balthica) food source and 2) current and tidal dynamics that keep foraging substrates accessible during all but extreme periods of cold and ice accretion. C. p. ptilocnemis is a subspecies of high conservation concern for which annual winter surveys may serve as a relatively inexpensive population-monitoring tool that will also provide insight into adaptations that allow these birds to exploit high-latitude environments in winter.

Alaska

Microhabitat selection, demography, and correlates of home range size for the King Rail ( Rallus elegans )

Animal movements and habitat selection within the home range, or microhabitat selection, can provide insights into habitat requirements, such as foraging and area requirements. The King Rail ( Rallus elegans ) is a wetland bird of high conservation concern in the United States, but little is known about its movements, habitats, or demography. King Rails ( n = 34) were captured during the 2010–2011 breeding seasons in the coastal marshes of southwest Louisiana and southeast Texas. Radio telemetry and direct habitat surveys of King Rail locations were conducted to estimate home ranges and microhabitat selection. Within home ranges, King Rails selected for greater plant species richness and comparatively greater coverage of Phragmites australis , Typha spp., and Schoenoplectus robustus . King Rails were found closer to open water compared to random locations placed 50 m from King Rail locations. Home ranges ( n = 22) varied from 0.8–32.8 ha and differed greatly among sites. Home range size did not vary by year or sex; however, increased open water, with a maximum of 29% observed in the study, was correlated with smaller home ranges. Breeding season cumulative survivorship was 89% ± 22% in 2010 and 61% ± 43% in 2011, which coincided with a drought. With an equal search effort, King Rail chicks and juveniles observed in May-June decreased from 110 in 2010 to only 16 in the drier year of 2011. The findings show King Rail used marsh with ≤ 29% open water and had smaller home ranges when open water was more abundant.

Louisiana, Texas

Predicted spatial distribution of the Eastern Spotted Skunk (Spilogale putorius) in Virginia using detection and non-detection records

The geographic distribution of a species is a fundamental component in understanding its ecology and is necessary for forming effective conservation plans. For rare and elusive species of conservation concern, accurate maps of predicted occurrence are particularly problematic and often highly subjective. Spilogale putorius (Eastern Spotted Skunk) populations have experienced large declines since the 1940s. Their elusive behavior and perceived rarity result in low detection probability when using conventional methods for sampling small mammals. Low detection probability often causes uncertainty as to where Eastern Spotted Skunks could be a management concern. We modeled the distribution of predicted occurrence of Eastern Spotted Skunks using verifiable occurrence and non-detection records obtained throughout Virginia from 2010 to 2020. Occurrence data consisted of trapping records reported to the Virginia Department of Wildlife Resources, incidental photo-verified reports of sightings and road-killed animals, and remote-camera detections. Non-detections were presumed at baited remote-camera locations following intense survey efforts. We fit predicted occurrence models using generalized linear modeling in an information-theoretic framework using the package ‘stats’ in Program R. Our results incidated a greater probability of presence from the Blue Ridge westward, increasing with slope steepness along northeastern- to southeastern-facing slopes and decreasing with slope steepness along southeastern- to southwestern-facing slopes. Emergent rock outcrops prominent along northeastern slopes offer ample protective rocky cover, whereas mixed Quercus spp. (oak), Kalmia latifolia (Mountain Laurel), and Rhododendron maximum (Rosebay Rhododendron) forest communities along southern-facing slopes provide suitable areas of cover, both of which are critical for spotted skunk survival and reproductive success. Our analysis provides insight into the relationships between landscape features and Eastern Spotted Skunk distributions across Virginia. Understanding these relationships is critical for the effective management and conservation of this vulnerable species.

Virginia

Age-0 Smallmouth Bass abundance depends on physicochemical conditions and stream network position

Stream fish survival and recruitment are products of a physicochemical environment that affects growth and provides refuge; yet, the drivers of spatiotemporal variation in juvenile fish abundance remain unclear. Understanding how physicochemical conditions drive spatial and temporal patterns in fish abundances provides insight into how conditions across stream networks influence fish population success, thereby providing direction to managers about the types and locations of conservation actions that would be most beneficial. Using snorkel and habitat surveys of 120 sites sampled from 2015 to 2017, we evaluated the multiscale relationships among physicochemical features, hydrology, and age-0 Smallmouth Bass ( Micropterus dolomieu velox ) abundance in relation to network spatial position. Abundance of age-0 bass was spatiotemporally variable in relation to a July streamflow–network position interaction, a pool depth–stream size interaction, and a stream temperature–network position interaction. High flows at the end of the nesting season were related to lower age-0 abundance, but this effect was dampened in stream reaches in close proximity to larger mainstems. In small streams, reaches with deeper pool habitat supported higher age-0 bass abundances, but this trend was not apparent in larger tributaries and mainstem systems. Generally, colder streams had lower age-0 Smallmouth Bass abundance, though this relationship was not apparent in reaches adjacent to larger streams that generally supported higher age-0 bass abundances. Conservation actions that (1) facilitate habitat connectivity within and among streams, (2) limit future anthropogenic practices that alter natural geomorphology by creating shallower stream channels, and (3) maintain adequate flow magnitude and timing to support channel complexity (e.g., deeper pools within smaller catchments) would be most beneficial to supporting rearing habitat for age-0 riverine Smallmouth Bass.

Arkansas, Missouri, Oklahoma

Divergent climate impacts on C3 versus C4 grasses imply widespread 21st century shifts in grassland functional composition

Aim Grasslands cover a third of Earth's landmass and provide critical ecosystem services. Anticipating how perennial C 3 (cool-season) and C 4 (warm-season) grasses respond to climate change will be key to predicting future composition and functioning of grasslands. Here, we evaluate environmental drivers of C 3 and C 4 perennial distributions and assess how C 3 and C 4 grass distributions shift in response to future climate change. Location Western United States. Methods We developed integrated species distribution models to identify climate and soil drivers of relative abundance of C 3 and C 4 perennial grasses. We then created projections of species abundances under future climate and evaluated when and where projected shifts in relative abundance were robust across climate models. Results Historically, C 3 grasses occupied areas with lower temperature and more variable precipitation regimes, while C 4 grasses occupied areas of higher temperature, greater temperature variability and greater warm-season precipitation. C 4 species also occupied narrower soil texture niches. In response to future climate change, C 3 grass abundance declined across 74% of areas, while C 4 abundance increased across 66% of areas. C 3 grasses expanded in mid- to higher-latitude areas with increasing temperature and decreasing seasonality of precipitation. In contrast, C 4 grasses increased in higher-latitude regions, but declined in lower-latitude, dryer regions. Results were surprisingly robust across climate scenarios, suggesting high confidence in the direction of these future changes. Main Conclusions Findings imply C 3 and C 4 perennial grasses will have highly divergent responses to climate change that may result in grassland functional compositional changes. Increasing temperatures and precipitation variability may favour some C 4 grasses, but C 4 habitat expansion may be constrained by soil conditions in western USA. Results provide actionable insights for anticipating the impacts of climate change on grass-dominated and co-dominated ecosystems and improving large-scale conservation and restoration efforts.

Diversity and Distributions

Feeding habits of the endangered Ozark big-eared bat (Corynorhinus townsendii ingens) relative to prey abundance

Feeding habits of the endangered Ozark big-cared bat (Corynorhinus townsendii ingens) in eastern Oklahoma, USA, were studied from July 1987 through July 1988. Diets were determined from microscopic analysis of fecal pellets and compared with arthropods collected in Malaise traps. Although lepidopterans comprised only 21.5% of the available prey, they occurred in > 90% of the pellets examined and accounted for > 85% of the volume of prey consumed. Dipterans, coleopterans, and homopterans occurred in 18.3%, 10,6%, and 6.7% of the feces, respectively, but each accounted for < 5% of the volume of prey consumed. Trichopterans, hymenopterans, and neuropterans also were found in feces but in trace amounts. Our results support the classification of C. t. ingens as a moth specialist, but additional insights are needed to fully understand how its feeding tactics conform to the allotonic frequency hypothesis (i.e., avoiding detection by cared moths). Conservation of this highly endangered North American bat will require, in part, maintenance of habitats capable of supporting abundant populations of Lepidoptera.

Acta Chiropterologica

Post-fire vegetation response in a repeatedly burned low-elevation sagebrush steppe protected area provides insights about resilience and invasion resistance

Sagebrush steppe ecosystems are threatened by human land-use legacies, biological invasions, and altered fire and climate dynamics. Steppe protected areas are therefore of heightened conservation importance but are few and vulnerable to the same impacts broadly affecting sagebrush steppe. To address this problem, sagebrush steppe conservation science is increasingly emphasizing a focus on resilience to fire and resistance to non-native annual grass invasion as a decision framework. It is well-established that the positive feedback loop between fire and annual grass invasion is the driving process of most contemporary steppe degradation. We use a newly developed ordinal zero-augmented beta regression model fit to large-sample vegetation monitoring data from John Day Fossil Beds National Monument, USA, spanning 7 years to evaluate fire responses of two native perennial foundation bunchgrasses and two non-native invasive annual grasses in a repeatedly burned, historically grazed, and inherently low-resilient protected area. We structured our model hierarchically to support inferences about variation among ecological site types and over time after also accounting for growing-season water deficit, fine-scale topographic variation, and burn severity. We use a state-and-transition conceptual diagram and abundances of plants listed in ecological site reference conditions to formalize our hypothesis of fire-accelerated transition to ecologically novel annual grassland. Notably, big sagebrush ( Artemisia tridentata ) and other woody species were entirely removed by fire. The two perennial grasses, bluebunch wheatgrass ( Pseudoroegneria spicata ) and Thurber's needlegrass ( Achnatherum thurberianum ) exhibited fire resiliency, with no apparent trend after fire. The two annual grasses, cheatgrass ( Bromus tectorum ) and medusahead ( Taeniatherum caput-medusae ), increased in response to burn severity, most notably medusahead. Surprisingly, we found no variation in grass cover among ecological sites, suggesting fire-driven homogenization as shrubs were removed and annual grasses became dominant. We found contrasting responses among all four grass species along gradients of topography and water deficit, informative to protected-area conservation strategies. The fine-grained influence of topography was particularly important to variation in cover among species and provides a foothold for conservation in low-resilient, aridic steppe. Broadly, our study demonstrates how to operationalize resilience and resistance concepts for protected areas by integrating empirical data with conceptual and statistical models.

Oregon

Hydrologic characteristics of freshwater mussel habitat: novel insights from modeled flows

The ability to model freshwater stream habitat and species distributions is limited by the spatially sparse flow data available from long-term gauging stations. Flow data beyond the immediate vicinity of gauging stations would enhance our ability to explore and characterize hydrologic habitat suitability. The southeastern USA supports high aquatic biodiversity, but threats, such as landuse alteration, climate change, conflicting water-resource demands, and pollution, have led to the imperilment and legal protection of many species. The ability to distinguish suitable from unsuitable habitat conditions, including hydrologic suitability, is a key criterion for successful conservation and restoration of aquatic species. We used the example of the critically endangered Tar River Spinymussel ( Parvaspina steinstansana ) and associated species to demonstrate the value of modeled flow data (WaterFALL™) to generate novel insights into population structure and testable hypotheses regarding hydrologic suitability. With ordination models, we: 1) identified all catchments with potentially suitable hydrology, 2) identified 2 distinct hydrologic environments occupied by the Tar River Spinymussel, and 3) estimated greater hydrological habitat niche breadth of assumed surrogate species associates at the catchment scale. Our findings provide the first demonstrated application of complete, continuous, regional modeled hydrologic data to freshwater mussel distribution and management. This research highlights the utility of modeling and data-mining methods to facilitate further exploration and application of such modeled environmental conditions to inform aquatic species management. We conclude that such an approach can support landscape-scale management decisions that require spatial information at fine resolution (e.g., enhanced National Hydrology Dataset catchments) and broad extent (e.g., multiple river basins).

North Carolina

Directional connectivity in hydrology and ecology

Quantifying hydrologic and ecological connectivity has contributed to understanding transport and dispersal processes and assessing ecosystem degradation or restoration potential. However, there has been little synthesis across disciplines. The growing field of ecohydrology and recent recognition that loss of hydrologic connectivity is leading to a global decline in biodiversity underscore the need for a unified connectivity concept. One outstanding need is a way to quantify directional connectivity that is consistent, robust to variations in sampling, and transferable across scales or environmental settings. Understanding connectivity in a particular direction (e.g., streamwise, along or across gradient, between sources and sinks, along cardinal directions) provides critical information for predicting contaminant transport, planning conservation corridor design, and understanding how landscapes or hydroscapes respond to directional forces like wind or water flow. Here we synthesize progress on quantifying connectivity and develop a new strategy for evaluating directional connectivity that benefits from use of graph theory in ecology and percolation theory in hydrology. The directional connectivity index (DCI) is a graph-theory based, multiscale metric that is generalizable to a range of different structural and functional connectivity applications. It exhibits minimal sensitivity to image rotation or resolution within a given range and responds intuitively to progressive, unidirectional change. Further, it is linearly related to the integral connectivity scale length—a metric common in hydrology that correlates well with actual fluxes—but is less computationally challenging and more readily comparable across different landscapes. Connectivity-orientation curves (i.e., directional connectivity computed over a range of headings) provide a quantitative, information-dense representation of environmental structure that can be used for comparison or detection of subtle differences in the physical-biological feedbacks driving pattern formation. Case-study application of the DCI to the Everglades in south Florida revealed that loss of directional hydrologic connectivity occurs more rapidly and is a more sensitive indicator of declining ecosystem function than other metrics (e.g., habitat area) used previously. Here and elsewhere, directional connectivity can provide insight into landscape drivers and processes, act as an early-warning indicator of environmental degradation, and serve as a planning tool or performance measure for conservation and restoration efforts.

Ecological Applications

Applications of a broad-spectrum tool for conservation and fisheries analysis: Aquatic gap analysis

Natural resources support all of our social and economic activities, as well as our biological existence. Humans have little control over most of the physical, biological, and sociological conditions dictating the status and capacity of natural resources in any particular area. However, the most rapid and threatening influences on natural resources typically are anthropogenic overuse and degradation. In addition, living natural resources (i.e., organisms) do not respect political boundaries, but are aware of their optimal habitat and environmental conditions. Most organisms have wider spatial ranges than the jurisdictional boundaries of environmental agencies that deal with them; even within those jurisdictions, information is patchy and disconnected. Planning and projecting effects of ecological management are difficult, because many organisms, habitat conditions, and interactions are involved. Conservation and responsible resource use involves wise management and manipulation of the aspects of the environment and biological communities that can be effectively changed. Tools and data sets that provide new insights and analysis capabilities can enhance the ability of resource managers to make wise decisions and plan effective, long-term management strategies. Aquatic gap analysis has been developed to provide those benefits. Gap analysis is more than just the assessment of the match or mis-match (i.e., gaps) between habitats of ecological value and areas with an appropriate level of environmental protection (e.g., refuges, parks, preserves), as the name suggests. Rather, a Gap Analysis project is a process which leads to an organized database of georeferenced information and previously available tools to examine conservation and other ecological issues; it provides a geographic analysis platform that serves as a foundation for aquatic ecological studies. This analytical tool box allows one to conduct assessments of all habitat elements within an area of interest. Aquatic gap analysis naturally focuses on aquatic habitats. The analytical tools are largely based on specification of the species-habitat relations for the system and organism group of interest (Morrison et al. 2003; McKenna et al. 2006; Steen et al. 2006; Sowa et al. 2007). The Great Lakes Regional Aquatic Gap Analysis (GLGap) project focuses primarily on lotic habitat of the U.S. Great Lakes drainage basin and associated states and has been developed to address fish and fisheries issues. These tools are unique because they allow us to address problems at a range of scales from the region to the stream segment and include the ability to predict species specific occurrence or abundance for most of the fish species in the study area. The results and types of questions that can be addressed provide better global understanding of the ecological context within which specific natural resources fit (e.g., neighboring environments and resources, and large and small scale processes). The geographic analysis platform consists of broad and flexible geospatial tools (and associated data) with many potential applications. The objectives of this article are to provide a brief overview of GLGap methods and analysis tools, and demonstrate conservation and planning applications of those data and tools. Although there are many potential applications, we will highlight just three: (1) support for the Eastern Brook Trout Joint Venture (EBTJV), (2) Aquatic Life classification in Wisconsin, and (3) an educational tool that makes use of Google Earth (use of trade or product names does not imply endorsement by the U.S. Government) and Internet accessibility.

Gap Analysis Bulletin