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At least 181 records · Page 10Linked to original sources

Potential role of acetyl-CoA synthetase (acs) and malate dehydrogenase (mae) in the evolution of the acetate switch in Bacteria and Archaea

Although many Archaea have AMP-Acs (acetyl-coenzyme A synthetase) and ADP-Acs, the extant methanogenic genus Methanosarcina is the only identified Archaeal genus that can utilize acetate via acetate kinase (Ack) and phosphotransacetylase (Pta). Despite the importance of ack as the potential urkinase in the ASKHA phosphotransferase superfamily, an origin hypothesis does not exist for the acetate kinase in Bacteria, Archaea, or Eukarya. Here we demonstrate that Archaeal AMP-Acs and ADP-Acs contain paralogous ATPase motifs previously identified in Ack, which demonstrate a novel relation between these proteins in Archaea. The identification of ATPase motif conservation and resulting structural features in AMP- and ADP-acetyl-CoA synthetase proteins in this study expand the ASKHA superfamily to include acetyl-CoA synthetase. Additional phylogenetic analysis showed that Pta and MaeB sequences had a common ancestor, and that the Pta lineage within the halophilc archaea was an ancestral lineage. These results suggested that divergence of a duplicated maeB within an ancient halophilic, archaeal lineage formed a putative pta ancestor. These results provide a potential scenario for the establishment of the Ack/Pta pathway and provide novel insight into the evolution of acetate metabolism for all three domains of life.

Scientific Reports

Estimation of volumetric runoff coefficients for Texas watersheds using land-use and rainfall-runoff data

The rational method for peak discharge ( Q p "> Q p ) estimation was introduced in the 1880s. Although the rational method is considered simplistic, it remains an effective method for estimating peak discharge for small watersheds. The runoff coefficient ( C "> C ) is a key parameter for the rational method and can be estimated in various ways. Literature-based C "> C values ( C lit "> C lit ) are listed for different land-use/land cover (two words, no hyphen) (LULC) conditions in various design manuals and textbooks; however, these C lit "> C lit values were developed with little basis on observed rainfall and runoff data. In this paper, C lit "> C lit values were derived for 90 watersheds in Texas by using LULC data for 1992 and 2001; the C lit "> C lit values derived from the two data sets were essentially the same. Also for this study, volumetric runoff coefficients ( C v "> C v ) were estimated by using observed rainfall and runoff depths from more than 1,600 events observed in the watersheds. Watershed-median and watershed-average C v "> C v values were computed, and both are consistent with data from the National Urban Runoff Program. In addition, C v "> C v values were estimated by using rank-ordered pairs of rainfall and runoff depths (i.e., frequency matching). As anticipated, C "> C values derived by all three methods (literature based, event totals, and frequency matching) consistently had larger values for developed watersheds than for undeveloped watersheds. Two regression equations of C v "> C v versus percent impervious area were developed and combined into a single equation that can be used to rapidly estimate C v "> C v values for similar Texas watersheds.

Texas

Return period adjustment for runoff coefficients based on analysis in undeveloped Texas watersheds

The rational method for peak discharge (Q p ) estimation was introduced in the 1880s. The runoff coefficient (C) is a key parameter for the rational method that has an implicit meaning of rate proportionality, and the C has been declared a function of the annual return period by various researchers. Rate-based runoff coefficients as a function of the return period, C(T), were determined for 36 undeveloped watersheds in Texas using peak discharge frequency from previously published regional regression equations and rainfall intensity frequency for return periods T of 2, 5, 10, 25, 50, and 100 years. The C(T) values and return period adjustments C(T)/C(T=10 year) determined in this study are most applicable to undeveloped watersheds. The return period adjustments determined for the Texas watersheds in this study and those extracted from prior studies of non-Texas data exceed values from well-known literature such as design manuals and textbooks. Most importantly, the return period adjustments exceed values currently recognized in Texas Department of Transportation design guidance when T>10 years.

Texas

Spatially robust estimates of biological nitrogen (N) fixation imply substantial human alteration of the tropical N cycle

Biological nitrogen fixation (BNF) is the largest natural source of exogenous nitrogen (N) to unmanaged ecosystems and also the primary baseline against which anthropogenic changes to the N cycle are measured. Rates of BNF in tropical rainforest are thought to be among the highest on Earth, but they are notoriously difficult to quantify and are based on little empirical data. We adapted a sampling strategy from community ecology to generate spatial estimates of symbiotic and free-living BNF in secondary and primary forest sites that span a typical range of tropical forest legume abundance. Although total BNF was higher in secondary than primary forest, overall rates were roughly five times lower than previous estimates for the tropical forest biome. We found strong correlations between symbiotic BNF and legume abundance, but we also show that spatially free-living BNF often exceeds symbiotic inputs. Our results suggest that BNF in tropical forest has been overestimated, and our data are consistent with a recent top-down estimate of global BNF that implied but did not measure low tropical BNF rates. Finally, comparing tropical BNF within the historical area of tropical rainforest with current anthropogenic N inputs indicates that humans have already at least doubled reactive N inputs to the tropical forest biome, a far greater change than previously thought. Because N inputs are increasing faster in the tropics than anywhere on Earth, both the proportion and the effects of human N enrichment are likely to grow in the future.

Proceedings of the National Academy of Sciences of

Overestimated natural biological nitrogen fixation translates to an exaggerated CO2 fertilization effect in Earth System Models

CO 2 fertilization of the terrestrial biosphere is limited by nitrogen. Biological nitrogen fixation (BNF) is the dominant natural nitrogen source to the terrestrial biosphere and can alleviate nitrogen limitation but is poorly constrained in Earth system models (ESMs). Here, we compare terrestrial BNF from an ensemble of ESMs of the 6th Coupled Model Intercomparison Project to a new global synthesis of observations across natural and agricultural biomes. We find that compared to observations, ESMs underestimate agricultural BNF but overestimate natural BNF in the present day by over 50%. Natural BNF is overestimated in the most productive ecosystems that contribute most to the terrestrial carbon sink (forests and grasslands). ESMs with different BNF representations yield a range of BNF responses to CO 2 enrichment. Some ESMs with phenomenological representations of BNF predict a natural BNF increase in response to a doubling of CO 2 that aligns with a meta-analysis of CO 2 enrichment experiments (31% increase) but fail to account for the substantial carbon cost of BNF. In contrast, ESMs with mechanistic representations of BNF account for its carbon cost as well as its regulation by nitrogen limitation but overestimate the BNF response to a doubling of CO 2 (135% increase). Overall, all current BNF representations in ESMs fall short of fully capturing its response to rising atmospheric CO 2 . Finally, we find a positive correlation between modeled present-day natural BNF and the CO 2 fertilization effect across ESMs, suggesting that overestimated natural BNF translates to an exaggerated CO 2 fertilization effect of approximately 11% in ESMs.

PNAS

Evaluation of the sensitivity of a federally endangered freshwater mussel (Venustaconcha trabalis) to selected chemicals

Protection of critically endangered species requires identification of factors limiting their survival and growth. Previous studies have demonstrated that unionid mussels are sensitive to some chemicals and the sensitivity was similar among different taxonomic families and tribes of mussels. However, common species of mussels were generally used in these previous studies; little is known about the sensitivity of endangered and threatened mussels relative to common species. The objective of this study was to evaluate the sensitivity of a critically endangered mussel (Tennessee bean, Venustaconcha trabalis ) to seven chemicals with different modes of toxic action (ammonia, chloride, nitrite, potassium, cobalt, manganese, nickel) in acute 96-h exposures and to three chemicals (nitrite, cobalt, iron) in chronic 28-d exposures conducted following standard methods. A commonly tested mussel (fatmucket, Lampsilis siliquoidea ) was also tested side-by-side with Tennessee bean in chronic exposures. Test chemicals were selected based on (1) chemicals of potential concern found in a review of existing data for the river where a population of Tennessee bean occurs or was historically present, (2) chemicals to which other mussels are sensitive, or (3) chemicals that had not been previously tested with mussels. Acute 50% effect concentrations (EC50s) for the seven chemicals from the Tennessee bean tests were within or close to the range of EC50s for other mussel species tested in previous studies, and chronic 20% effect concentrations for the three chemicals were similar between Tennessee bean and fatmucket, indicating the endangered species has sensitivity similar to other tested mussel species. Inclusion of the new mussel data in existing toxicity databases for freshwater organisms would rank one or more mussel species among the four most sensitive species to ammonia, chloride, potassium, and nickel in acute exposures and to nitrite, cobalt, and iron in chronic exposures.

Environmental Toxicology and Chemistry

Effects of dietary selenium on the amphipod Hyalella azteca and the midge Chironomus dilutus

Chronic selenium (Se) water quality criteria are based primarily on dietary organoselenium exposure and subsequent reproductive effects in fish. Available chronic Se toxicity data suggests that invertebrates are less sensitive than fish, but chronic invertebrate studies are limited. We evaluated yeast-based diets for chronic toxicity studies with Hyalella azteca and Chironomus dilutus. Growth and survival were similar among C. dilutus fed a yeast-only diet, a mixture of yeast and fish flake food, and a mixture of yeast and diatoms. Survival and growth of H. azteca were best in the yeast + diatom diet, so this diet was used for subsequent tests. In rangefinder tests, H. azteca 28-day survival, growth, and biomass, and C. dilutus 11-day survival were all significantly reduced at a dietary Se concentration of 49 µg/g dry weight, whereas C. dilutus growth and biomass were only affected at 200 µg/g dry weight Se. H. azteca had similar sensitivity to dietary Se in a 28–42-day chronic test—survival, growth, and biomass were significantly reduced at 44.5 µg/g dry weight Se. Dietary selenium reduced H. azteca reproduction by 75%–100% relative to Controls, but differences were not significant. Tissue Se EC10s were lower for H. azteca (9.0–23 µg/g dry weight) than for C. dilutus (11–56 µg/g dry weight). However, model evaluations suggest that the C. dilutus 11-day survival and H. azteca 42-day EC10s were less reliable than other endpoints. When tissue EC10s were converted to equivalent Se concentrations in fish tissue with a food web model, H. azteca (18 µg/g dry weight) and C . dilutus (68 µg/g dry weight) ranked 6th and 12th of 13 freshwater genera, respectively. Overall, these results suggest that the current U.S. Environmental Protection Agency tissue-based Se water quality criteria would be protective for fish and invertebrates and could be used for management of fishless waters.

Environmental Toxicology and Chemistry

Biota dose assessment of small rodents sampled near breccia pipe uranium mines in the Grand Canyon watershed

The biotic exposure and uptake of radionuclides and potential health effects due to breccia pipe uranium mining in the Grand Canyon watershed are largely unknown. This paper describes the use of the RESRAD-BIOTA dose model to assess exposure of small rodents (n = 11) sampled at three uranium mine sites in different stages of ore production (active and postproduction). Rodent tissue and soil concentrations of naturally occurring uranium (234U, 235U, and 238U), thorium (228Th, 230Th, and 232Th), and radium (226Ra) radioisotopes were used in the dose model. The dose assessment results indicated that the potential internal, external, and total doses to rodents were below the US Department of Energy’s biota dose standard of 1 mGy d−1. As expected, tissue concentrations of 238U, 234U, and 230Th were in approximate equilibrium; however, 226Ra results in tissue were 1.25 to 5.75 times greater than 238U, 234U, and 230Th tissue results for 10 out of 11 samples. Soil at the three sites also displayed 226Ra enrichment, so it is likely that the 226Ra enrichment in the rodents was from soil via typical activities (i.e., burrowing, incidental ingestion, bathing, etc.) or by dietary uptake of translocated 226Ra. The results suggest that 226Ra is more mobile in this environment and bioaccumulates in these rodent species (e.g., in bones via the bloodstream). Internal dose accounting suggests that 226Ra is the radionuclide of most concern for rodent exposure and health.

Grand Canyon

Biological nitrogen fixation: rates, patterns and ecological controls in terrestrial ecosystems

New techniques have identified a wide range of organisms with the capacity to carry out biological nitrogen fixation (BNF)—greatly expanding our appreciation of the diversity and ubiquity of N fixers—but our understanding of the rates and controls of BNF at ecosystem and global scales has not advanced at the same pace. Nevertheless, determining rates and controls of BNF is crucial to placing anthropogenic changes to the N cycle in context, and to understanding, predicting and managing many aspects of global environmental change. Here, we estimate terrestrial BNF for a pre-industrial world by combining information on N fluxes with 15 N relative abundance data for terrestrial ecosystems. Our estimate is that pre-industrial N fixation was 58 (range of 40–100) Tg N fixed yr −1 ; adding conservative assumptions for geological N reduces our best estimate to 44 Tg N yr −1 . This approach yields substantially lower estimates than most recent calculations; it suggests that the magnitude of human alternation of the N cycle is substantially larger than has been assumed.

Philosophical Transactions of the Royal Society B:

A roadmap for sampling and scaling biological nitrogen fixation in terrestrial ecosystems

Accurately quantifying rates and patterns of biological nitrogen fixation (BNF) in terrestrial ecosystems is essential to characterize ecological and biogeochemical interactions, identify mechanistic controls, improve BNF representation in conceptual and numerical modelling, and forecast nitrogen limitation constraints on future carbon (C) cycling. While many resources address the technical advantages and limitations of different methods for measuring BNF, less systematic consideration has been given to the broader decisions involved in planning studies, interpreting data, and extrapolating results. Here, we present a conceptual and practical road map to study design, study execution, data analysis and scaling, outlining key considerations at each step. We address issues including defining N‐fixing niches of interest, identifying important sources of temporal and spatial heterogeneity, designing a sampling scheme (including method selection, measurement conditions, replication, and consideration of hotspots and hot moments), and approaches to analysing, scaling and reporting BNF. We also review the comparability of estimates derived using different approaches in the literature, and provide sample R code for simulating symbiotic BNF data frames and upscaling. Improving and standardizing study design at each of these stages will improve the accuracy and interpretability of data, define limits of extrapolation, and facilitate broader use of BNF data for downstream applications. We highlight aspects—such as quantifying scales of heterogeneity, statistical approaches for dealing with non‐normality, and consideration of rates versus ecological significance—that are ripe for further development.

Methods in Ecology and Evolution

Relationships among net primary productivity, nutrients and climate in tropical rain forest: A pan-tropical analysis

Tropical rain forests play a dominant role in global biosphere-atmosphere CO 2 exchange. Although climate and nutrient availability regulate net primary production (NPP) and decomposition in all terrestrial ecosystems, the nature and extent of such controls in tropical forests remain poorly resolved. We conducted a meta-analysis of carbon-nutrient-climate relationships in 113 sites across the tropical forest biome. Our analyses showed that mean annual temperature was the strongest predictor of aboveground NPP (ANPP) across all tropical forests, but this relationship was driven by distinct temperature differences between upland and lowland forests. Within lowland forests (< 1000 m), a regression tree analysis revealed that foliar and soil-based measurements of phosphorus (P) were the only variables that explained a significant proportion of the variation in ANPP, although the relationships were weak. However, foliar P, foliar nitrogen (N), litter decomposition rate (k), soil N and soil respiration were all directly related with total surface (0–10 cm) soil P concentrations. Our analysis provides some evidence that P availability regulates NPP and other ecosystem processes in lowland tropical forests, but more importantly, underscores the need for a series of large-scale nutrient manipulations – especially in lowland forests – to elucidate the most important nutrient interactions and controls.

Ecology Letters

Stoichiometric patterns in foliar nutrient resorption across multiple scales

*Nutrient resorption is a fundamental process through which plants withdraw nutrients from leaves before abscission. Nutrient resorption patterns have the potential to reflect gradients in plant nutrient limitation and to affect a suite of terrestrial ecosystem functions. *Here, we used a stoichiometric approach to assess patterns in foliar resorption at a variety of scales, specifically exploring how N : P resorption ratios relate to presumed variation in N and/or P limitation and possible relationships between N : P resorption ratios and soil nutrient availability. *N : P resorption ratios varied significantly at the global scale, increasing with latitude and decreasing with mean annual temperature and precipitation. In general, tropical sites (absolute latitudes < 23°26′) had N : P resorption ratios of < 1, and plants growing on highly weathered tropical soils maintained the lowest N : P resorption ratios. Resorption ratios also varied with forest age along an Amazonian forest regeneration chronosequence and among species in a diverse Costa Rican rain forest. *These results suggest that variations in N : P resorption stoichiometry offer insight into nutrient cycling and limitation at a variety of spatial scales, complementing other metrics of plant nutrient biogeochemistry. The extent to which the stoichiometric flexibility of resorption will help regulate terrestrial responses to global change merits further investigation.

New Phytologist

Melanoma and other melanistic lesions in brown bullhead Ameiurus nebulosus from waterbodies in the northeastern United States and Canada: Identification of risk factors

Melanistic lesions, including non-raised black areas due to proliferations of melanocytes and melanomacrophages in the dermis and epidermis, as well as raised black areas consistent with melanoma, are described in brown bullhead (BBH) Ameiurus nebulosus from three water bodies in the northeastern United States and Quebec, Canada. First observed in the Vermont portion of Lake Memphremagog, Vermont, USA and Quebec, Canada, the prevalence of melanistic lesions during 2014–2020 was greater than 30% in BBH 200 mm and longer. In 2023, seven sites throughout the lake were assessed, and prevalence ranged from 18% to 42%. In Hermon Pond, Maine, the prevalence was 29% in 2024, and in Village Pond, New Hampshire, lesions occurred in 22% of BBH in 2025. Compared to skin from visibly normal BBH, skin with melanistic lesions had significantly higher concentrations of seven metals, including arsenic, a known carcinogen and zinc. Lesions associated with oxidative damage, such as the accumulation of ceroid/lipofuscin, were also observed in the gill, spleen and kidney tissue of both affected and visibly normal BBH. The progression of lesions, observed by histopathology, ranged from inflammation, signs of oxidative damage, proliferation and necrosis of club cells, and the presence of melanomacrophages and melanocytes in the epidermis to invasive melanoma and suggests chronic exposure of BBH to environmental initiators and promoters of carcinogenesis.

New Hampshire, New York, Quebec, Vermont

Plutonium speciation in water from Mono Lake, California

The solubility of plutonium in Mono Lake water is enhanced by the presence of large concentrations of indigenous carbonate ions and moderate concentrations of fluoride ions. In spite of the complex chemical composition of this water, only a few ions govern the behavior of plutonium, as demonstrated by the fact that it was possible to duplicate plutonium speciation in a synthetic water containing only the principal components of Mono Lake water.

California

Latitudinal variation in snowshoe hare (Lepus americanus) body mass: A test of Bergmann’s Rule

The relationship between body size and latitude have been the focus of dozens of studies across many species. However, results of testing Bergmann’s Rule – that organisms in colder climates or at higher latitudes possess larger body sizes – have been inconsistent across studies. We investigated whether snowshoe hares (Lepus americanus) follow the Rule by investigating differences in body mass using data from six published studies and from data of 755 individual hares captured from ten populations across North America covering 26° of latitude. We also explored alternative hypotheses related to variation in hare body mass, including winter severity, length of growing season, elevation, and snow depth. We found body mass of hares varied throughout their range, but the drivers of body mass differed based on geographic location. Females in northern populations followed Bergmann’s rule, whereas males did not. In northern populations male mass was related to average snow depth. In contrast, in southern populations body mass of both sexes was related to length of the growing season. These differences likely represent variation in the drivers of selection. Specifically, in the north, a large body size is beneficial to conserve heat because of low winter temperatures, whereas in the south, it is likely due to increased food supply associated with longer growing seasons.

Canadian Journal of Zoology

Enabling real-time user interaction for decision support: Experiences extending a local agave platform metadata service

The University of Hawai'i Information Technology Services Cyber-infrastructure team in partnership with the United States Geological Survey developed the Hawai'i groundwater recharge tool, a decision support tool, as part of the 'Ike Wai Gateway to support water sustainability research for the state of Hawai'i. To enable the development of the tool within the existing University of Hawai'i Agave platform that the 'Ike Wai Gateway is built on, the Metadata application programmatic interface (API) and Agave infrastructure needed to be extended to store and retrieve spatial data and handle a larger number of requests. The development team successfully added the functionality to the local University of Hawai'i Agave platform instance to support the storage of deserialized vector data and metadata that can be spatially queried across hundreds of thousands of records to enable decision support applications within a science gateway.

Hawaii

Functional ecology of free-living nitrogen fixation: A contemporary perspective

Nitrogen (N) availability is thought to frequently limit terrestrial ecosystem processes, and explicit consideration of N biogeochemistry, including biological N 2 fixation, is central to understanding ecosystem responses to environmental change. Yet, the importance of free-living N 2 fixation—a process that occurs on a wide variety of substrates, is nearly ubiquitous in terrestrial ecosystems, and may often represent the dominant pathway for acquiring newly available N—is often underappreciated. Here, we draw from studies that investigate free-living N 2 fixation from functional, physiological, genetic, and ecological perspectives. We show that recent research and analytical advances have generated a wealth of new information that provides novel insight into the ecology of N 2 fixation as well as raises new questions and priorities for future work. These priorities include a need to better integrate free-living N 2 fixation into conceptual and analytical evaluations of the N cycle's role in a variety of global change scenarios.

Annual Review of Ecology, Evolution, and Systemati