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Water-quality characteristics of selected lakes and reservoirs in Colorado

During the summers and autumns of 1973, 1974, and 1975, water-quality data were collected and compiled for 56 lakes and reservoirs in Colorado. The lakes and reservoirs sampled range from large reservoirs in the eastern part of the State to smaller natural lakes in the higher western parts of the Colorado Plateaus. The smallest body of water sampled was Anderson Reservoir No. 1 with a surface area of 47 acres in Mesa County, and the largest was Najajo Reservoir with a surface area of 15,600 acres in Archuleta County. The report includes physical, chemical, and biological water-quality data collected as a part of each lake or reservoir reconnaissance. Also included are data on drainage area, water-surface altitude, surface area,depth, storage capacity, and principal uses, obtained from various governmental agencies or the owner or regulating agency of each lake or reservoir. A subjective evaluation of the trophic (enrichment) state is presented based on nutrient and phytoplankton concentrations and the dissolved-oxygen distribution. In addition, a numerical trophic-state index, based on Secchi-disk transparency (a measure of light penetration) is reported, to show trophic comparisons between lakes and reservoirs. (USGS)

Open-File Report

Cell-Based metabolomics for untargeted screening and prioritization of vertebrate-active stressors in streams across the United States

The U.S. Geological Survey and the U.S. Environmental Protection Agency have assessed contaminants in 38 streams across the U.S., using an extensive suite of target-chemical analysis methods along with a variety of biological effects tools. Here we report zebrafish liver (ZFL) cell-culture based NMR metabolomic analysis of these split stream samples. We used this untargeted approach to evaluate the sites according to overall impact on the ZFL metabolome and found that neither the total number of organics detected at the sites, nor their cumulative concentrations, were good predictors of these impacts. Further, we used partial-least squares regression to compare ZFL endogenous metabolite profiles to values for 455 potential stressors (organics, inorganics, and physical properties) measured in these waters and found that the profiles covaried with at most 280 of the stressors, which were subsequently ranked into quartiles based on the strength of their covariance. While contaminants of emerging concern (CECs) were well represented in the top, most strongly, covarying quartile – suggesting considerable potential for eliciting biological responses at these sites – there was even higher representation of various well-characterized legacy contaminants (e.g., PCBs). These results emphasize the importance of complementing chemical analysis with untargeted bioassays to help focus regulatory efforts on the most significant ecosystem threats.

Environmental Science & Technology

Surficial geology of the lower Comb Wash, San Juan County, Utah

The surficial geologic map of lower Comb Wash was produced as part of a master’s thesis for Northern Arizona University Quaternary Sciences program. The map area includes the portion of the Comb Wash alluvial valley between Highway 163 and Highway 95 on the Colorado Plateau in southeastern Utah. The late Quaternary geology of this part of the Colorado Plateau had not previously been mapped in adequate detail. The geologic information in this report will be useful for biological studies, land management and range management for federal, state and private industries. Comb Wash is a south flowing ephemeral tributary of the San Juan River, flanked to the east by Comb Ridge and to the west by Cedar Mesa (Figure 1). The nearest settlement is Bluff, about 7 km to the east of the area. Elevations range from 1951 m where Highway 95 crosses Comb Wash to 1291 m at the confluence with the San Juan River. Primary vehicle access to lower Comb Wash is provided by a well-maintained dirt road that parallels the active channel of Comb Wash between Highway 163 and Highway 95. For much of the year this road can be traversed without the aid of four-wheel drive. However, during inclement weather such as rain or snow the road becomes treacherous even with four-wheel drive. The Comb Wash watershed is public land managed by the Bureau of Land management (BLM) office in Monticello, Utah. The semi-arid climate of Comb Wash and the surrounding area is typical of the Great Basin Desert. Temperature in Bluff, Utah ranges from a minimum of –8° C in January to a maximum of 35° C in July with a mean annual temperature of 9.8° C (U.S. Department of Commerce, 1999). The difference between day and nighttime temperatures is as great as 20° C. Between 1928 and 1998, annual rainfall in Bluff averaged 178 mm per year (U.S. Department of Commerce, 1999). Annual rainfall in Comb Wash averaged 240 mm per year from 1991 to 1999 while Bluff received an average of 193 mm for the same 8 year period. Most precipitation is monsoonal, convective storms that bring moisture from the Gulf of Mexico beginning in early July and ending by October. Large frontal storms during December and January are responsible for most winter precipitation (Figure 2). The record from U.S. Geological Survey gauging station number 09379000 operated by the BLM from 1959 through 1968 indicates that Comb Wash flows in direct response to precipitation events. Most daily discharge and peak events occur in late July through September, coinciding with high intensity monsoon thunderstorms. Comb Wash supports a variety of vegetation typical of the Great Basin Desert and the northern desert shrub zone as described by Fowler and Koch (1982). On the lower alluvial terraces, bushes and shrubs dominate the vegetation, including: sagebrush (Artemesia tridentata), rabbitbrush (Chrysothamnus nauseosus), fourwing saltbush (Atriplex canescens), winterfat (Eurotia lanata), greasewood (Sarcobatus vermiculatus), and shadscale (Atriplex concertifolia). Juniper trees (Juniperus osteosperma) can be found on the rocky colluvial slopes near Comb Ridge and on the higher terrace near Cedar Mesa. The floodplain contains an abundance of riparian vegetation including cottonwood (Populus fremontii), willow (Salix exigua), and tamarisk (Tamarix ramosissima). Tamarisk is one of 7 non-native species present in the lower Comb Wash watershed. At least seven known species of noxious weeds have invaded the watershed, including Bermuda grass (Cynodon dactylon), field bindweed (Convolvulus avensis), Canada thistle (Cirsium arvense), Russian knapweed (Centaurea repens), tamarisk and camel thorn (Alhagi pseudalhagi). Of these, tamarisk or salt-cedar has most aggressively colonized the southwestern United States, including the San Juan watershed. Graf (1978) estimates that since the late 19th century, tamarisk has spread at a rate of 20 km per year. Tamarisk first appeared in Comb Wash during the mid to early 20th century based on photographs taken by Gregory in the early 1900’s (Gregory, 1938).

Utah

Agency approach to allocation of human capital and program composition for human-marine mammal interaction management in the United States

Introduction: Marine mammals have served important and diverse roles throughout human history. Once exploited to near extinction, many species in the United States (U.S.) have recovered dramatically under the Marine Mammal Protection Act (MMPA). The U.S. National Marine Fisheries Service (NMFS) has jurisdiction to administer the MMPA for cetaceans and most pinnipeds. While most take is now prohibited, NMFS still dedicates significant time and resources to protecting and conserving marine mammals. As human populations continue to grow and exploit marine environments, opportunities for human-marine mammal interactions (HMMI) have increased. Resources may fail to keep up with growing needs for conservation investments, so that many agencies struggle to adequately meet social and legal requirements. Methods: This study evaluated NMFS’ approach to addressing HMMI through a mixed-methods (semi-structured interviews, quantitative questionnaire, and facilitated workshops) expert elicitation analysis of resource allocation (i.e., staff time) and priorities of NMFS Science Center (SC) and Regional Office (RO) staff across five geographic regions to understand how employee time is allocated, the degree to which employee time allocation is aligned with self-reported job descriptions, and employee perceptions of actions or investments which would improve their ability to mitigate HMMI. Results and Discussion: SC staff reported spending more time on Biological and Administrative tasks , while RO staff allocated more time to Policy, Social science , and Outreach tasks . SC employees spent less time on Biological tasks than specified by their perceived job descriptions, suggesting resource constraints. Participants identified Biological research and monitoring as top priorities, followed by Policy tasks . Identified actions included increasing research capacity, enhancing enforcement, expanding social science expertise, and improving outreach programs. The study reveals mismatches between mandated activities and available resources, suggesting strategic reallocation or increased funding may benefit the agency in effectively addressing growing HMMI challenges.

Frontiers in Marine Science

Occurrence and Distribution of Organochlorine Compounds in Biological Tissue and Bed Sediment From Streams in the Trinity River Basin, Texas, 1992-93

This report describes the occurrence and distribution of organochlorine compounds in biological tissue and bed sediment from the Trinity River Basin study area of the National Water-Quality Assessment Program. Concentrations of organochlorine pesticides, polychlorinated biphenyls (PCBs), and other organochlorine compounds were determined in biological tissue and surficial bed sediment from 16 stream sites in the Trinity River Basin of east-central Texas. Asiatic clams (Corbicula fluminea) were collected at 10 sites, and fish, including blue catfish (Ictalurus furcatus), common carp (Cyprinus carpio), bluegill (Lepomis cyanellus), and yellow bullhead (Ameiurus natalis) were collected at all mainstem and two tributary sites. Thirty of the 36 compounds analyzed in biological tissue or surficial bed sediment were detected in one or both media. Overall, more organochlorine compounds were detected in bed sediment than in biological tissue; however, various chlordane isomers, DDT metabolites, and PCBs were detected more frequently in tissue than in sediment. The chlordane isomers and PCBs that were detected more frequently in biological tissue also were detected more frequently at urban sites than at agricultural sites. Organochlorine compound concentrations generally were highest in fish tissue from Trinity River mainstem sites. Fish tissue from the mainstem sites contained a higher percentage of lipids than did fish- and clam-tissue samples from the tributary sites.

Water-Resources Investigations Report

Assessing biological effects from highway-runoff constituents

Increased emphasis on evaluation of nonpoint-source pollution has intensified the need for techniques that can be used to discern the toxicological effects of complex chemical mixtures. In response, the use of biological assessment techniques is receiving increased regulatory emphasis. When applied with documented habitat assessment and chemical analysis, these techniques can increase our understanding of the influence of environmental contaminants on the biological integrity and ecological function of aquatic communities. The contaminants of greatest potential concern in highway runoff are those that arise from highway construction, maintenance, and use. The major contaminants of interest are deicers; nutrients; metals; petroleum-related organic compounds, such as polycyclic aromatic hydrocarbons (PAHs), benzene, toluene, ethylbenzene, and xylene (BTEX), and methyl tert -butyl ether (MTBE); sediment washed off the road surface; and agricultural chemicals used in highway maintenance. Hundreds, if not thousands, of biological endpoints (measurable responses of living organisms) may be either directly or associatively affected by contaminant exposure. Measurable effects can occur throughout ecosystem processes across the wide range of biological complexity, ranging from responses at the biochemical level to the community level. The challenge to the environmental scientist is to develop an understanding of the relationship of effects at various levels of biological organization in order to determine whether a causal relationship exists between chemical exposure and substantial ecological impairment. This report provides a brief history of the evolution of biological assessment techniques, a description of the major classes of contaminants that are of particular interest in highway runoff, an overview of representative biological assessment techniques, and a discussion of data-quality considerations. Published reports with a focus on the effects of highway runoff on the local ecosystem were reviewed to provide information on (1) the suitability of the existing data for a quantitative national synthesis, (2) the methods available to study the effects of highway runoff on local ecosystems, and (3) the potential for adverse effects on the roadside environment and receiving waters. Although many biological studies have been done, the use of different methods and a general lack of sufficient documentation precludes a quantitative national synthesis on the basis of the existing data. The Federal Highway Administration, the U.S. Environmental Protection Agency, the U.S. Geological Survey, the Intergovernmental Task Force on Monitoring Water Quality, and the National Resources Conservation Service all have developed and documented methods for assessing the effects of contaminants on ecosystems in receiving waters. These published methods can be used to formulate a set of protocols to provide consistent information from highway-runoff studies. Review of the literature indicates (qualitatively) that highway runoff (even from highways with high traffic volume) may not usually be acutely toxic. Tissue analysis and community assessments, however, indicate effects from highway- runoff sediments near discharge points (even from sites near highways with relatively low traffic volumes). At many sites, elevated concentrations of highway-runoff constituents were measured in tissues of species associated with aquatic sediments. Community assessments also indicate decreases in the diversity and productivity of aquatic ecosystems at some sites receiving highway runoff. These results are not definitive, however, and depend on many site-specific criteria that were not sufficiently documented in most of the studies reviewed.

Open-File Report

Assessing mercury exposure and effects to American dippers in headwater streams near mining sites

To evaluate mercury (Hg) exposure and possible adverse effects of Hg on American dipper ( Cinclus mexicanus ) reproduction, we collected eggs and nestling feathers and the larval/nymph form of three Orders of aquatic macroinvertebrates (Ephemeroptera, Plecoptera and Trichoptera = EPT) important in their diet from three major headwater tributaries of the upper Willamette River, Oregon in 2002. The Coast Fork Willamette River is contaminated with Hg due to historical cinnabar (HgS) mining at the Black Butte Mine; the Row River is affected by past gold-mining operations located within the Bohemia Mining District, where Hg was used in the amalgamation process to recover gold; and the Middle Fork Willamette River is the reference area with no known mining. Methyl mercury (MeHg) concentrations (geometric mean) in composite EPT larvae (111.9 ng/g dry weight [dw] or 19.8 ng/g wet weight [ww]), dipper eggs (38.5 ng/g ww) and nestling feathers (1158 ng/g ww) collected from the Coast Fork Willamette were significantly higher than MeHg concentrations in EPT and dipper samples from other streams. Total mercury (THg) concentrations in surface sediments along the same Hg-impacted streams were investigated by others in 1999 (Row River tributaries) and 2002 (Coast Fork). The reported sediment THg concentrations paralleled our biological findings. Dipper breeding territories at higher elevations had fewer second clutches; however, dipper reproductive success along all streams (including the lower elevation and most Hg-contaminated Coast Fork), was judged excellent compared to other studies reviewed. Furthermore, MeHg concentrations in EPT samples from this study were well below dietary concentrations in other aquatic bird species, such as loons and ducks, reported to cause Hg-related reproductive problems. Our data suggest that either dipper feathers or EPT composites used to project MeHg concentrations in dipper feathers (with biomagnification factor of 10–20×) may be used, but with caution, to screen headwater streams for potential Hg-related effects on dippers. When actual feather concentrations or projected feather concentrations are equal to or lower than concentrations reported for the Coast Fork, dippers are expected to reproduce well (assuming adequate prey and suitable nest sites). When Hg concentrations are substantially higher, more detailed investigations may be required. Birds feeding almost exclusively on fish (e.g., osprey [ Pandion haliaetus ]) and usually found further downstream from the headwaters would not be adequately represented by dippers given the higher MeHg concentrations in fish resulting from biomagnification, compared to lower trophic level invertebrates.

Oregon

Identification of larval Pacific lampreys (Lampetra tridentata), river lampreys (L. ayresi), and western brook lampreys (L. richardsoni) and thermal requirements of early life history stages of lampreys. Annual report 2002-2003

Two fundamental aspects of lamprey biology were examined to provide tools for population assessment and determination of critical habitat needs of Columbia River Basin (CRB) lampreys (the Pacific lamprey, Lampetra tridentata, and the western brook lamprey, L. richardsoni). We evaluated the usefulness of current diagnostic characteristics for identification of larval lampreys (i.e., pigment patterns) and collected material for development of meristic and morphometric descriptions of early life stage CRB lampreys, and we determined the effects of temperature on survival and development of early life stage CRB lampreys. Thirty-one larval lampreys were collected from locations throughout the CRB and transported to the Columbia River Research Laboratory. Lampreys were sampled at six-week intervals at which time they were identified to the species level based on current diagnostic characteristics. Sampling was repeated until lampreys metamorphosed, at which time species identification was validated based on dentition, or until they died, at which time they were preserved for genetic examination. These lampreys were sampled 30 times with two individuals metamorphosing, both of which were consistently identified, and subsequently validated, as Pacific lampreys. Of the remaining lampreys, only one was inconsistently identified (Pacific lamprey in 83% of the sampling events and western brook lamprey in 17% of the sampling events). These data suggest that pigmentation patterns do not change appreciably through time. In 2001 and 2002 we artificially spawned Pacific and western brook lampreys in the laboratory to provide material for meristic and morphometric descriptions. We collected, digitized, preserved, and measured the mean chorion diameter of Pacific and western brook lamprey embryos. Embryos ranged in development from 1 d post fertilization to just prior to hatch, and were incubated at 14 C. Mean chorion diameter was greater and more variable for Pacific lampreys (mean {+-} SD; 1.468 {+-} 0.107 mm, N = 320) than for western brook lampreys (1.237 {+-} 0.064 mm, N = 280). An unpaired t-test showed that the difference in mean chorion diameter between species was highly significant (t = 32.788, df = 528.62, P < 0.0001). For larvae, we collected, digitized, and preserved 156 individuals from each species. Eight homologous landmarks defining a two-cell truss network with two appended triangles were selected for morphometric analyses and species discrimination. A full model discriminant analysis correctly classified 92% of the Pacific lampreys and 93% of the western brook lampreys in a classification data set. When applied to a test data set, the classification functions correctly classified 91% of the Pacific lampreys and 85% of the western brook lampreys. A backward elimination discriminant analysis removed four variables from the full model, and the reduced model correctly classified 91% of the Pacific lampreys and 93% of the western brook lampreys in a classification data set. The reduced model classification functions correctly classified 91% of the Pacific lampreys and 85% of the western brook lampreys in a test data set. In 2001 and 2002 Pacific and western brook lampreys were artificially spawned and resulting progeny were reared in the laboratory at 10 C, 14 C, 18 C, and 22 C. The estimated temperature for zero development was 4.85 C for Pacific and 4.97 C for western brook lampreys. Survival was greatest at 18 C followed by 14 C, 10 C, and 22 C, with significant differences observed between 22 C and other temperatures. Overall survival was significantly greater for western brook than for Pacific lampreys, although the difference in proportion of individuals surviving was only 0.02. Survival to hatch was significantly greater than survival to the larval stage with a difference of only 0.03. The proportion of individuals exhibiting abnormalities at the larval stage was greatest at 22 C followed by 18 C, 10 C, and 14 C, with significant differences observed between 22 C and other temperatures.

Report

Populations, productivity, and feeding habits of seabirds at Cape Thompson, Alaska: Final report

Investigations of seabird population sizes and breeding biology were conducted at Cape Thompson from 1959 to 1961 during pre-development studies associated with the Atomic through 1982, the Alaskan Program (OCSEAP) supported determine whether changes Energy Commission’s “Project Chariot.” From 1976 Outer Continental Shelf Environmental Assessment efforts to recensus seabirds at Cape Thompson and had occurred since the 1959-61 period. Prior to the present study, it had been 6 years since the last efforts to census seabird colonies in this area. We established a field camp at the and occupied it continuously until 31 were selected for cliff nesting species comprising the Cape Thompson complex, mouth of Ikijaktusak Creek on 2 July August 1988. Permanent study plots in four of the five discrete colonies and regular observations were made throughout the study to document attendance patterns, breeding phenology, and success of murres and kittiwakes. Periodic collections of adults offshore were used to determine the food habits of study species. Shore-based work was supplemented with offshore studies of seabird foraging from the USFWS vessel Eagle-Tiglax, 24-31 August (Fig. 2). Correlation analysis revealed negative trends in murre attendance at all Cape Thompson colonies between 1960 and 1982 or 1988, significantly so for 3 of the 5 colonies. Based on apparent changes in species composition within the colonies, Common Murres declined at a more rapid rate than Thick-billed Murres between 1960 and 1988. Combining information from all colonies, it appears that murre populations have been relatively stable since about 1979. In contrast to murres, the kittiwake population showed no significant trends between 1960 and 1982 or between 1960 and 1988. All fluctuations in kittiwake numbers documented between years were within the variability expected within years. Breeding productivity of murres was about average during 1988 (0.47 young/pair), whereas the productivity of kittiwakes was very poor (0.15 young/pair). Murres and kittiwakes fed mostly on arctic cod and sand lance distributed widely but in low concentrations (e.g., 0.1-10 g/m 3 ) UP to 1.20 km north and northwest of Cape Thompson. In the total area surveyed (225 km 2 ), only two major feeding aggregations were observed where fish school densities exceeded 15 g/ins. Forage fish densities were higher in shallow Alaska Coastal Current waters than offshore in Bering Sea waters, and piscivorous seabirds like murres and kittiwakes fed mostly in coastal waters. Reduced numbers of fish in murre and kittiwake stomachs in August and low breeding success of kittiwakes suggested that forage fish densities observed around Cape Thompson in late August were sufficient to sustain murres but were insufficient for, or inaccessible to, kittiwakes. The breeding failure of Black-legged Kittiwakes at Cape Thompson in 1988 was part throughout causes of kittiwakes of a pervasive syndrome of failure in this species observed the Bering/Chukchi seas and Gulf of Alaska in recent years. The recurrent widespread breeding failure need to be identified if are to have a role in area-wide population monitoring during the period of Alaskan OCS development by the oil and gas industry. The system of land-based plots established in 1988 is recommended for future population monitoring of cliff-nesting birds at Cape Thompson. Based on the coefficients of variation among counts observed in this study, it is estimated that 10 replicate counts per year would detect an 8% change in numbers of Thick-billed Murres between years and a 12% change in Common Murres, with 75% certainty of statistical significance at the 0.05 level. Similarly, a 9% annual change in the population of Black-legged Kittiwakes should be detectable at the 0.05 significance level given samples of 10 replicate counts of the land-based plots.

Alaska

Endocrine active chemicals, pharmaceuticals, and other chemicals of concern in surface water, wastewater-treatment plant effluent, and bed sediment, and biological characteristics in selected streams, Minnesota: Design, methods, and data, 2009

This report presents the study design, environmental data, and quality-assurance data for an integrated chemical and biological study of selected streams or lakes that receive wastewater-treatment plant effluent in Minnesota. This study was a cooperative effort of the U.S. Geological Survey, the Minnesota Pollution Control Agency, St. Cloud State University, the University of St. Thomas, and the University of Colorado. The objective of the study was to identify distribution patterns of endocrine active chemicals, pharmaceuticals, and other organic and inorganic chemicals of concern indicative of wastewater effluent, and to identify biological characteristics of estrogenicity and fish responses in the same streams. The U.S. Geological Survey collected and analyzed water, bed-sediment, and quality-assurance samples, and measured or recorded streamflow once at each sampling location from September through November 2009. Sampling locations included surface water and wastewater-treatment plant effluent. Twenty-five wastewater-treatment plants were selected to include continuous flow and periodic release facilities with differing processing steps (activated sludge or trickling filters) and plant design flows ranging from 0.002 to 10.9 cubic meters per second (0.04 to 251 million gallons per day) throughout Minnesota in varying land-use settings. Water samples were collected from the treated effluent of the 25 wastewater-treatment plants and at one point upstream from and one point downstream from wastewater-treatment plant effluent discharges. Bed-sediment samples also were collected at each of the stream or lake locations. Water samples were analyzed for major ions, nutrients, trace elements, pharmaceuticals, phytoestrogens and pharmaceuticals, alkylphenols and other neutral organic chemicals, carboxylic acids, and steroidal hormones. A subset (25 samples) of the bed-sediment samples were analyzed for carbon, wastewater-indicator chemicals, and steroidal hormones; the remaining samples were archived. Biological characteristics were determined by using an in-vitro bioassay to determine total estrogenicity in water samples and a caged fish study to determine characteristics of fish from experiments that exposed fish to wastewater effluent in 2009. St. Cloud State University deployed and processed caged fathead minnows at 13 stream sites during September 2009 for the caged fish study. Measured fish data included length, weight, body condition factor, and vitellogenin concentrations.

Minnesota

Physical, chemical, and biological characteristics of Sturgeon Lake, Goodhue County, Minnesota, 2003-04

The U.S. Geological Survey, in cooperation with the Prairie Island Indian Community and the Minnesota Department of Natural Resources, conducted a study of Sturgeon Lake (a backwater lake in Navigation Pool 3 of the Mississippi River) during 2003-04 to describe the physical, chemical, and biological characteristics of the lake. Riparian and shoreline areas surrounding Sturgeon Lake consist primarily of deciduous tree and shrub cover with minimal amounts of commercial or residential land use. Woody debris and aquatic vegetation are the major types of physical habitat suitable for fish and invertebrates. Among 10 bottom-sediment sampling sites, 24 organic wastewater compounds, 1 organochlorine pesticide metabolite (p,p&rsquo;DDE), and total polychlorinated biphenyls (PCBs) were detected in the bottom sediments of Sturgeon Lake. The most prevalent class of compounds detected were polyaromatic hydrocarbons. Other classes of compounds detected include sterols, disinfectants, plastic components, alkylphenols, and fragrances. Three compounds detected (bisphenol A, benzo[a]pyrene, and triclosan) are considered endocrine disrupting compounds. Twenty-one and 49 invertebrate taxa were identified from 10 bottom-sediment and 6 woody-debris/vegetation samples, respectively. Most of the taxa were Diptera in the family Chironomidae. The most common invertebrate in terms of density in bottom-sediment samples was the burrowing mayfly ( Hexagenia sp.). Trichoptera in the families Hydropsychidae or Polycentropodidae were common in most of the woody-debris samples. The presence of the Hexagenia larvae in samples indicates that the bottom sediments are stable and that dissolved oxygen concentrations in the lake do not drop to acute or sub-lethal anoxic conditions. Backwater lakes such as Sturgeon Lake are important areas of habitat for aquatic organisms along the Mississippi River, and this report provides baseline physical, chemical, and biological information that resource managers can compare with future investigations.

Minnesota

U.S. Geological Survey applied research studies of the Cheyenne River System, South Dakota; description and collation of data, water years 1987-88

The Cheyenne River System in western South Dakota has been impacted by the discharge of about 100 million metric tons of gold-mill tailings to Whitewood Creek near Lead, South Dakota. In April 1985, the U.S. Geological Survey initiated an extensive series of research studies to investigate the magnitude of the impact and to define important processes acting on the contaminated sediments present in the system. The report presents all data collected during the 1987 and 1988 water years for these research studies. Some of the data included have been published previously. Data collected in the 1985 and 1986 water years have been published in a companion report (U.S. Geological Survey Open-File Report 88-484). Hydrologic, geochemical, and biologic data are available for sites on Whitewood Creek, and the Belle Fourche and Cheyenne Rivers. Data complexity varies from routine discharge and water-quality to very complex energy-dispersive x-ray analysis. Methods for sample collection, handling and preservation, and laboratory analysis are also presented. No interpretations or complex statistical summaries are included. (See also W89-08390) (USGS)

Open-File Report

Limnological data for the major streams in Chester County, Pennsylvania

Limnological data on major streams in Chester County, Pennsylvania are tabulated to provide a base line as to the present stream conditions. As land-use patterns change and further urbanization takes place, it is anticipated that these data will serve as a basis for comparison of conditions in the future. The 13 basins encompass a total drainage area of 1,812 km2 (697 mi2) of the 1,976 km2 (760 mi2) within Chester County. Four of the streams are tributaries to the Schuylkill River and include Pickering, Stony Run, Pigeon, and French Creek. Tributaries to the Delaware River include Darby, Crum, Ridley, Chester, Red Clay, and White Clay Creeks. The Elk Creeks flow into the Chesapeake Bay. The Brandywine is a tributary to the Christiana River, itself a tributary to the Delaware. The Octoraro Creek is a tributary to the Susquehanna River. Chester County, which is located in the Piedmont area of Pennsylvania, is undergoing rapid transition from a rural to suburban condition as the population increases. This basic-data report contains the chemical, physical, and biological information collected for the period fall 1969 through fall 1974. The network of 50 stations is shown in figure 1, which is followed by a listing (table 1) of station numbers and a brief description of station locations. Table 2 shows the physical, chemical, and bacteriological data collected by station and date. Table 3 shows the benthic invertebrate data collected by station and date.

Pennsylvania

The influence of biological soil crusts on mineral uptake by associated vascular plants

Soil surfaces dominated by cyanobacteria and cyanolichens (such as Collema sp.) are widespread in deserts of the world. The influence of these biological soil crusts on the uptake of bioessential elements is reported for the first time for six seed plants of the deserts of Utah. This sample almost doubles the number of species for which the influence of biological soil crusts on mineral uptake of associated vascular plants is known. These new case studies, and others previously published, demonstrate that cyanobacterial or cyanobacteria- Collema crusts significantly alter uptake by plants of many bioessential elements. In studies now available, these crusts always increase the N content of associated seed plants. Uptake of Cu, K, Mg, and Zn is usually (>70% of reported cases) increased in the presence of the biological soil crusts. Soil crusts are generally negatively associated with Fe and P levels in associated seed plant tissue, while plant tissue levels of Ca, Mn, and Na are positively as often as negatively associated with the presence of soil crusts. Increases in bioessential elements in vascular plant tissue from biologically-crusted areas are greatest for short-lived herbs that are rooted primarily within the surface soil, the horizon most influenced by crustal organisms. The mineral content of a deeply rooted shrub ( Coleogyne ramosissima ) was less influenced by co-occurrence of biological soil crusts.

Utah

Quality of Streams in Johnson County, Kansas, and Relations to Environmental Variables, 2003-07

The quality of streams and relations to environmental variables in Johnson County, northeastern Kansas, were evaluated using water, streambed sediment, land use, streamflow, habitat, algal periphyton (benthic algae), and benthic macroinvertebrate data. Water, streambed sediment, and macroinvertebrate samples were collected in March 2007 during base flow at 20 stream sites that represent 11 different watersheds in the county. In addition, algal periphyton samples were collected twice (spring and summer 2007) at one-half of the sites. Environmental data including water and streambed-sediment chemistry data (primarily nutrients, fecal-indicator bacteria, and organic wastewater compounds), land use, streamflow, and habitat data were used in statistical analyses to evaluate relations between biological conditions and variables that may affect them. This report includes an evaluation of water and streambed-sediment chemistry, assessment of habitat conditions, comparison of biological community attributes (such as composition, diversity, and abundance) among sampling sites, placement of sampling sites into impairment categories, evaluation of biological data relative to environmental variables, and evaluation of changes in biological communities and effects of urbanization. This evaluation is useful for understanding factors that affect stream quality, for improving water-quality management programs, and for documenting changing conditions over time. The information will become increasingly important for protecting streams in the future as urbanization continues. Results of this study indicate that the biological quality at nearly all biological sampling sites in Johnson County has some level of impairment. Periphyton taxa generally were indicative of somewhat degraded conditions with small to moderate amounts of organic enrichment. Camp Branch in the Blue River watershed was the only site that met State criteria for full support of aquatic life in 2007. Since 2003, biological quality improved at one rural sampling site, possibly because of changes in wastewater affecting the site, and declined at three urban sites possibly because of the combined effects of ongoing development. Rural streams in the western and southern parts of the county, with land-use conditions similar to those found at the State reference site (Captain Creek), continue to support some organisms normally associated with healthy streams. Several environmental factors contribute to biological indicators of stream quality. The primary factor explaining biological quality at sites in Johnson County was the amount of urbanization upstream in the watershed. Specific conductance of stream water, which is a measure of dissolved solids in water and is determined primarily by the amount of groundwater contributing to streamflow, the amount of urbanization, and discharges from wastewater and industrial sites, was strongly negatively correlated with biological stream quality as indicated by macroinvertebrate metrics. Concentration of polycyclic aromatic hydrocarbons (PAHs) in streambed sediment also was negatively correlated with biological stream quality. Individual habitat variables that most commonly were positively correlated with biological indicators included stream sinuosity, buffer length, and substrate cover diversity. Riffle substrate embeddedness and sediment deposition commonly were negatively correlated with favorable metric scores. Statistical analysis indicated that specific conductance, impervious surface area (a measure of urbanization), and stream sinuosity explained 85 percent of the variance in macroinvertebrate communities. Management practices affecting environmental variables that appear to be most important for Johnson County streams include protection of stream corridors, measures that reduce the effects of impervious surfaces associated with urbanization, reduction of dissolved solids in stream water, reduction of PAHs entering streams and

Scientific Investigations Report