USGS2002
Samples of nitrogen-source material, soil, and water were collected from several small, primarily single-source subbasins in the Lower Susquehanna River Basin during 1988–90 to determine the feasibility of using measurements of stable isotope ratios of carbon ( 13 C), nitrogen ( 15 N), and sulfur ( 34 S) to identify sources of nitrogen (N) in stream water. Chemical and isotopic compositions were measured for six N-source types consisting of rain water, forest leaf litter, synthetic fertilizer, farm-animal manure, municipal-sewage effluent and sludge, and septictank effluent and sludge. Compositions of associated, nearby samples of topsoil, subsoil, runoff water, and stream water were measured to evaluate changes in compositions of transported N-containing materials near the N source. Animal manure, human waste (sewage plus septic), and forest-leaf litter can be distinguished on the basis of 13 C; however, most N-sources cannot be distinguished on the basis of 15 N and 34 S, owing to wide ranges and overlap of compositions among different N-source types. Although values of 15 N for soil and runoff-water samples are qualitatively similar to those of the applied N source, values of 13 C and 34 S for runoff-water and stream-water samples appear to reflect the compositions of relatively large reservoirs of the elements in soil organic matter and minerals, respectively, and not the composition of the applied N source. The ratio of organic carbon to total nitrogen (C-org:N), combined with 13 C, is useful for distinguishing agricultural soils, which have characteristically high 13 C and low C-org:N, from forested soils. The C-org:N values of suspended particulates in runoff or stream waters generally are lower than those of nearby soils, however, and indicate that chemical transformations and resultant isotopic fractionation can be important controls on the compositions of N-containing compounds in the soil and water. In aqueous samples including surface water and liquid N-sources, isotopic ratios commonly differ between coexisting dissolved fractions of NO 3- N and NH 3- N and between dissolved and particulate fractions of N or S, probably because of isotopic fractionation during transport or N-source processing. Isotopic measurements provide qualitative information about important reactions that can affect N concentrations in surface waters. However, mass-balance computations generally are not sufficiently accurate to estimate the proportions of multiple sources contributing to the N load in the streams studied because of (1) variations in source chemical and isotopic compositions and (2) nonconservative behavior and fractionation during transport over short distances (hundreds of meters). Uncertainties in mass-balance computations are complicated by the propagation of errors associated with measurements of discharge, chemical concentrations,and isotopic compositions of relatively dilute, small streams.