A methodology for quantifying trace elements in the exoskeletons of Florida stone crab ( Menippe mercenaria ) larvae using inductively coupled plasma optical emission spectrometry (ICP–OES)

Philip M. Gravinese; Jennifer A. Flannery; Lauren T. Toth

doi:10.3133/ofr20161148

Home/Archive/USGS

USGS · ofr20161148

A methodology for quantifying trace elements in the exoskeletons of Florida stone crab ( Menippe mercenaria ) larvae using inductively coupled plasma optical emission spectrometry (ICP–OES)

Philip M. Gravinese·Jennifer A. Flannery·Lauren T. Toth

View original source ↗Read source document ↗Export metadata ↓

Abstract

The larvae of the Florida stone crab, Menippe mercenaria , migrate through a variety of habitats as they develop and, therefore, experience a broad range of environmental conditions through ontogeny. Environmental variability experienced by the larvae may result in distinct elemental signatures within the exoskeletons, which could provide a tool for tracking the environmental history of larval stone crab populations. A method was developed to examine trace-element ratios, specifically magnesium-to-calcium (Mg/Ca) and strontium-to-calcium (Sr/Ca) ratios, in the exoskeletons of M. mercenaria larvae. Two developmental stages of stone crab larvae were analyzed—stage III and stage V. Specimens were reared in a laboratory environment under stable conditions to quantify the average ratios of Mg/Ca and Sr/Ca of larval stone crab exoskeletons and to determine if the ratios differed through ontogeny. The elemental compositions (Ca, Mg, and Sr) in samples of stage III larvae (n = 50 per sample) from 11 different broods (mean Sr/Ca = 5.916 ± 0.161 millimole per mole [mmol mol −1 ]; mean Mg/Ca = 218.275 ± 59.957 mmol mol −1 ) and stage V larvae (n = 10 per sample) from 12 different broods (mean Sr/Ca = 6.110 ± 0.300 mmol mol −1 ; mean Mg/Ca = 267.081 ± 67.211 mmol mol –1 ) were measured using inductively coupled plasma optical emission spectrometry (ICP–OES). The ratio of Sr/Ca significantly increased from stage III to stage V larvae, suggesting an ontogenic shift in Sr/Ca ratios between larval stages. The ratio of Mg/Ca did not change significantly between larval stages, but variability among broods was high. The method used to examine the trace-element ratios provided robust, highly reproducible estimates of Sr/Ca and Mg/Ca ratios in the larvae of M. mercenaria , demonstrating that ICP–OES can be used to determine the trace-element composition of chitinous organisms like the Florida stone crab.

Explore related subjects

Open-File Report Report

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Philip M. Gravinese, Jennifer A. Flannery, Lauren T. Toth. 2016-11-23. A methodology for quantifying trace elements in the exoskeletons of Florida stone crab ( Menippe mercenaria ) larvae using inductively coupled plasma optical emission spectrometry (ICP–OES). https://doi.org/10.3133/ofr20161148

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related USGS reports

USGS2026

ECCOE Landsat quarterly calibration and validation report—Quarter 2, 2026

Open-File Report↗

USGS2026

ECCOE Landsat quarterly calibration and validation report—Quarter 1, 2026

Executive Summary The U.S. Geological Survey Earth Resources Observation and Science Calibration and Validation (Cal/Val) Center of Excellence (ECCOE) focuses on improving the accuracy, precision, calibration, and product quality of remote-sensing data, leveraging years of multiscale optical system geometric and radiometric calibration and characterization experience. The ECCOE Landsat Cal/Val Team continually monitors the geometric and radiometric performance of active Landsat missions and makes calibration adjustments, as needed, to maintain data quality at the highest level. This report provides observed geometric and radiometric analysis results for Landsats 8 and 9 for quarter 1 (January–March) of 2026. All data used to compile the Cal/Val analysis results presented in this report are freely available from the U.S. Geological Survey EarthExplorer website: https://earthexplorer.usgs.gov . One specific activity that the ECCOE Landsat Cal/Val Team closely monitored was a Landsat 8 Thermal Infrared Sensor encoder alerts anomaly. On March 18, 2026, the Thermal Infrared Sensor instrument’s mechanism control electronics board reset, driving several parameters to default power-on values. Subsequent telemetry and analysis determined that no persistent hardware misconfiguration was present and no radiometric or geometric degradation was observed.

Open-File Report↗

USGS2026

Floods of June 20–July 6, 2024, in the Big Sioux River, Rock River, Little Sioux River, Ocheyedan River, and Floyd River Basins, northwestern Iowa

Major flooding occurred on June 20–July 6, 2024, in northwestern Iowa affecting the Big Sioux, Rock, Little Sioux, Ocheyedan, and Floyd River Basins. Heavy rain fell in northwestern Iowa, southwestern Minnesota, and southeastern South Dakota on June 20–22, 2024. Parts of northwestern Iowa recorded 2–6 inches of rainfall and localized amounts exceeding 12 inches. A maximum peak-of-record streamflow of 175,000 cubic feet per second at the U.S. Geological Survey streamgage Big Sioux River at Akron, Iowa (06485500), was recorded on June 22, 2024, and had an annual exceedance probability range of 0.2–0.49 percent. High-water marks were measured at four locations along the Big Sioux River between U.S. Interstate 29 at Sioux City, Iowa, upstream to Iowa Highway 10 north of Hawarden, Iowa, a distance of 75.7 river miles. A maximum peak-of-record streamflow of 157,000 cubic feet per second at the U.S. Geological Survey streamgage Rock River near Rock Valley, Iowa (06483500), was recorded on June 22, 2024, and had an annual exceedance probability of less than 0.2 percent. High-water marks were measured at eight locations along the Rock River between County Road B30 east of Hudson, South Dakota, upstream to Iowa Highway 9 at Rock Rapids, Iowa, a distance of 39.3 river miles. A maximum peak-of-record streamflow of 63,000 cubic feet per second at the U.S. Geological Survey streamgage Little Sioux River at Correctionville, Iowa (06606600), was recorded on June 24, 2024, and had an annual exceedance probability range of 0.2–0.49 percent. High-water marks were measured at 11 locations along the Little Sioux River between Iowa Highway 31 west of Correctionville, Iowa, upstream to U.S. Highway 18 north of Spencer, Iowa, a distance of 134.8 river miles. A maximum streamflow of 24,500 cubic feet per second at the U.S. Geological Survey streamgage Ocheyedan River near Spencer, Iowa (06605000), was recorded on June 22, 2024, and had an annual exceedance probability range of 0.2–0.49 percent. High-water marks were measured at three locations along the Ocheyedan River between County Road M38 west of Spencer, Iowa, upstream to U.S. Highway 18 west of Everly, Iowa, a distance of 12.8 river miles. A maximum streamflow of 41,000 cubic feet per second at the U.S. Geological Survey streamgage Floyd River at Alton, Iowa (06600100), was recorded on June 22, 2024, and had an annual exceedance probability range of 1–1.99 percent. High-water marks were measured at six locations along the Floyd River between Iowa Highway 3 at Le Mars, Iowa, upstream to Iowa Highway 10 at Alton, Iowa, a distance of 27.5 river miles. The high-water marks were used to develop flood profiles for the Big Sioux, Rock, Little Sioux, Ocheyedan, and Floyd Rivers.

Iowa, Minnesota, South Dakota↗