Detection and genetic characterization of red-spotted grouper nervous necrosis virus and a novel genotype of nervous necrosis virus in black sea bass from the U.S. Atlantic coast

Abstract

Nervous necrosis virus (NNV) causes a neurologic disease in a wide range of marine fish and poses serious disease risks to marine aquaculture worldwide. Little is known about the presence of NNV along the Atlantic coast of the United States, aside from the presence of barfin flounder nervous necrosis virus (BFNNV) in coldwater species in the northern part of this range. Herein we conducted surveillance for NNV from 2020 to 2022 in the mid-Atlantic region of the United States in black sea bass Centropristis striata , a serranid fish that is found throughout the eastern U.S. coast. Molecular detection methods have identified and characterized red-spotted grouper nervous necrosis virus (RGNNV) sequences at low prevalence throughout the years. Further, in 2022, a higher prevalence of a novel NNV genotype, tentatively named black sea bass nervous necrosis virus (BSBNNV), was characterized for the first time. Though virus isolation was unsuccessful, this study was the first to genetically identify NNV in this region and in this species. These findings highlight the need for further research on NNV to understand epidemiology and virulence in the context of marine fisheries and an emerging marine aquaculture industry in the United States.