Nonsalmonid gas bubble trauma investigations

Abstract

From 2020 to 2023, a new spill program was implemented to aid the downstream passage of juvenile salmonids at mainstem dams on the Snake and Columbia rivers. Under this program, the total dissolved gas (TDG) cap was increased to 125% and monitoring of nonsalmonids for gas bubble trauma (GBT) became a requirement. The primary objective of this work and report was to measure the incidence and severity of GBT in nonsalmonids resulting from increased juvenile salmonid passage spill and associated levels of TDG during the spring spill period in 2023. Nonsalmonids were collected downstream from Bonneville, McNary, Ice Harbor, and Lower Granite dams and examined for the incidence and severity of GBT in 2023. Fish were collected at each location weekly (3 April to 20 June) during the spring spill period by backpack electrofishing and beach seining. Washington and Oregon State water quality agencies established minimum and target sample sizes for monitoring, but the minimum sample size of 50 fish and target sample size of 100 fish were not met in all weeks at individual projects due to high water flows and resulting low fish collections. Collected fish were examined for GBT according to the criteria and protocol established for the regional smolt monitoring program (SMP). TDG levels were often high relative to the 10-year average. GBT incidence rates and severity (according to SMP criteria) were low to moderate in most weeks. We found no apparent relationship between GBT incidence and TDG due to exposure history and interspecies susceptibility to elevated TDG that could not be quantified. GBT incidence rates exceeded the 15% threshold on two occasions below Ice Harbor Dam, triggering a reduction in spill under the State water quality standards. In the weeks immediately following the spill reductions, GBT incidence was zero or relatively low at this location. Sculpin (genus Cottus ) was the main species collected at all locations. As in past years, we did find GBT in non-SMP protocol areas, particularly in sculpin. The variability in GBT incidence rates is likely due to variability in environmental conditions, fish exposure history to TDG, and species sensitivity to TDG. Many of the species encountered in shallow shoreline habitats rear for extended times and probably do not seek the water depths that would help them reduce the effects of exposure to elevated TDG through depth compensation. Limited systematic sampling of TDG in the tailraces of each project showed that TDG can vary spatially within the tailrace and with percentage of water spilled. We investigated GBT progression and mortality in sculpin and threespine stickleback ( Gasterosteus aculeatus ) in laboratory experiments (Chapter Two of this report). Fish were tested at 120%, 125%, and 130% TDG. We found that sculpin are more sensitive to TDG than stickleback and that GBT and associated mortality progress faster in sculpin than in stickleback. GBT prevalence and severity increased through time at all TDG levels tested, but relationships between severity and exposure time were weak or nonexistent. GBT and mortality progressed more rapidly as TDG increased in both species. The SMP criteria used to rank GBT did not fully capture the incidence and severity of GBT in sculpin and stickleback compared to using criteria based on all areas of the fish. The lateral line, body, dorsal fin, and pectoral fins were common locations of GBT in sculpin at 120 and 125% TDG, but signs were more prevalent in all areas at 130% TDG. In stickleback, GBT was most common on the head and body at all TDG levels tested. Positive buoyancy of fish with severe GBT was observed in both species and may have consequences for similarly impaired fish in the wild. The proximate cause of GBT-related death in sculpin and stickleback was bubbles in the gills and heart, but unlike in other species, bubbles appeared rapidly just before the point of death. Our results help fill the information void GBT progression and mortality for sculpin and stickleback.