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Disinfection protocols for herpetofaunal pathogens

Molly C. Bletz·James T. Julian·Megan S. Kirchgessner·James M. Drasher·Paula F. P. Henry·Susan D. Jewell·Pamela T. Meier·Kathy Michelle·Jennifer C. Olori·Kevin J. Oxenrider·Michael J. Ravesi·Scott A. Smith
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Abstract

The spread of disease-causing pathogens is a major threat to amphibians and reptiles worldwide (Converse and Greene 2005; Picco et al. 2007; Picco and Collins 2008; St-Amour et al. 2008; O’Hanlon et al. 2018; Scheele et al. 2019). The World Organisation for Animal Health’s global list of notifiable animal diseases includes herpetofaunal diseases caused by infection with Ranavirus spp. (RV) and two chytrid fungi, Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal; WOAH 2023). Scientists began testing the efficacy of disinfection protocols even before human-assisted transmission of these pathogens was documented (Johnson et al. 2003; Johnson and Speare 2003; Brem et al. 2007; Bryan et al. 2009), and now guidance exists to determine the appropriate biosecurity measures as personal gear moves between habitats, construction equipment moves between project areas, and investigators handle multiple animals within a population (Phillot et al. 2010; Gray et al. 2017, 2018; Julian et al. 2020; Olson et al. 2021). People who are engaged in educational, recreational, commercial, or professional activities in wetlands and aquatic habitats play an important role in helping prevent the spread of pathogens and should know how and when to employ appropriate disinfecting procedures. In particular, working groups and regional chapters of Partners in Amphibian and Reptile Conservation (PARC) have developed a variety of educational materials on biosecurity and disinfection for a wide audience (e.g., PARC 2023). Herein, we provide the instructions for the disinfection of field equipment that were recently revised by the Emerging Disease Working Group of Northeast PARC (NEPARC 2022). The impetus for revision was to inform field personnel of the use of a stronger bleach concentration (ca. 10× stronger) that is needed for effectiveness against Bsal (Van Rooij et al. 2017). This is important for regions where Bsal is currently known to occur (e.g., central Europe) as well as regions where it is presumed absent but could arrive at any time (e.g., North America)

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Molly C. Bletz, James T. Julian, Megan S. Kirchgessner, James M. Drasher, Paula F. P. Henry, Susan D. Jewell, Pamela T. Meier, Kathy Michelle, Jennifer C. Olori, Kevin J. Oxenrider, Michael J. Ravesi, Scott A. Smith. 2023. Disinfection protocols for herpetofaunal pathogens. https://pubs.usgs.gov/publication/70249970

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No detection of Batrachochytrium salamandrivorans after non-native salamander putative pet release in Massachusetts, USA

The disease caused by the amphibian chytrid fungus Batrachochytrium salamandrivorans (Bsal) has caused mass mortality of salamanders across Europe (Stegen et al. 2017; amphibians are not known to be susceptible to the disease (Martel et al. 2014). Bsal puts North American amphibian biodiversity at risk (Martel et al. 2014; Gray et al. 2015) despite imports of potentially infected amphibians (USFWS 2016; WAPTR 2018; Grear et al. 2021). Recently, the U.S. has announced the finalization of the 2016 interim rule to the Lacey Act (USFWS 2016) to restrict imports of all species of salamanders from 20 genera to the list of injurious amphibians and has added 16 genera to the injurious amphibian list in a new interim rule in response to updated research on salamander susceptibility to Bsal infection (USFWS 2025). A large-scale survey across the USA failed to detect Bsal in over 10,000 individuals (Waddle et al. 2020), and ongoing efforts by North American colleges and universities are similarly conducting Bsal surveillance without more recent detection (e.g., Hyman and Hill 2024). Concerned amphibian and disease specialists nevertheless are preparing for a Bsal invasion into North America (Grant et al. 2017; Olson et al.2024). Despite the initial survey results, the risk of Bsal incursion into North America remains due to broad host susceptibility of North American amphibian species (Gray et al. 2023). The major driver of Bsal introduction risk in the U.S. is expected to be captive amphibian release (Cunningham et al. 2015; Grear et al. 2021). Bsal primarily affects salamanders but anuran species can also be vectors (e.g., Towe et al. 2021; Monzon et al. 2022; Gray et al. 2023; Olson et al. 2024), increasing the risk of a released amphibian carrying Bsal into the natural environment (Grear et al. 2021). An early survey of pet salamanders in the United States did not detect Bsal (Klocke et al. 2017), but even one animal released with Bsal could have a major effect on local amphibians. Early detection and rapid response can improve host outcomes (Bletz et al. 2024; Gray et al. 2018), but the lack of timely knowledge about unwanted release of non-native pets to natural systems causes inevitable delay. Herein, we describe our experience responding to a report of a released non-native amphibian species, common in the pet trade (Gerson 2012; Maximo et al. 2021). We suggest improvements to early detection and rapid response efforts for amphibians and amphibian disease

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