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USGS · 70182034

Na+, K+-activated-ATPase inhibition in rainbow trout: A site for organochlorine pesticide toxicity?

Paul W. Davis·Gary A. Wedemeyer
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Abstract

1. The Na + , K + -activated, Mg 2+ -dependent-ATPase enzyme system in a heavy microsomal fraction of rainbow trout ( Salmo gairdneri ) brain was inhibited in vitro by chlorinated hydrocarbon pesticides. 2. T 50 (concentration at 50 per cent inhibition) values for dicofol, endosulfan and DDT were 5 × 10 −6 , 3 × 10 −5 and 1 × 10 −4 M respectively. Similar inhibition by these pesticides occurred in kidney and gill ATPase preparations. 3. An unexpected finding was a failure of the classic inhibitor, ouabain, to block the Na + , K + -activated component of ATPase activity in the gill. 4. It is suggested that inhibition of ATPase activity may be a causal factor in the toxic effects of organochlorine pesticides in fishes.

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Comparative Biochemistry and Physiology, Part B: Comparative BiochemistryArticle

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BibTeXRIS

Paul W. Davis, Gary A. Wedemeyer. 1971. Na+, K+-activated-ATPase inhibition in rainbow trout: A site for organochlorine pesticide toxicity?. https://doi.org/10.1016/0305-0491(71)90157-x

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