Development of eighteen microsatellite loci in walleye ( Sander vitreus )

D. Katharine Coykendall; Cheryl L. Morrison; Wendylee Stott; Marcus J. Springmann

doi:10.1007/s12686-014-0275-8

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Development of eighteen microsatellite loci in walleye ( Sander vitreus )

D. Katharine Coykendall·Cheryl L. Morrison·Wendylee Stott·Marcus J. Springmann

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Abstract

A suite of tri- and tetra-nucleotide microsatellite loci were developed for walleye ( Sander vitreus ) from 454 pyrosequencing data. Eighteen of the 50 primer sets tested amplified consistently in 35 walleye from two lakes on Isle Royale, Lake Superior: Chickenbone Lake and Whittlesey Lake. The loci displayed moderate levels of allelic diversity (average 5.5 alleles/locus) and heterozygosity (average 35.8 %). Levels of genetic diversity were sufficient to produce unique multi-locus genotypes and detect phylogeographic structuring as individuals assigned back to their population of origin. Cross-species amplification within S. canadensis (sauger) was successful for 15 loci, and 11 loci were diagnostic to species. The loci characterized here will be useful for detecting fine-scale spatial structuring, resolving the taxonomic status of Sander species and sub-species, and detecting walleye/sauger hybrids.

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Source-reported bounding extent: 48.001517° to 48.076659° latitude; -88.730243° to -88.687804° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

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D. Katharine Coykendall, Cheryl L. Morrison, Wendylee Stott, Marcus J. Springmann. 2014-07-20. Development of eighteen microsatellite loci in walleye ( Sander vitreus ). https://doi.org/10.1007/s12686-014-0275-8

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