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Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology

Julia P. Galkiewicz·Christina A. Kellogg
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Abstract

PCR amplification of pure bacterial DNA is vital to the study of bacterial interactions with corals. Commonly used Bacteria -specific primers 8F and 27F paired with the universal primer 1492R amplify both eukaryotic and prokaryotic rRNA genes. An alternative primer set, 63F/1542R, is suggested to resolve this problem.

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Applied and Environmental MicrobiologyArticle

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Julia P. Galkiewicz, Christina A. Kellogg. 2008. Cross-kingdom amplification using Bacteria-specific primers: Complications for studies of coral microbial ecology. https://doi.org/10.1128/aem.01303-08

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