Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site

T. R. Dombrowski; E.M. Thurman; G. B. Mohrman

doi:10.1021/bk-1997-0657.ch018

Home/Archive/USGS

USGS · 70018126

Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site

T. R. Dombrowski·E.M. Thurman·G. B. Mohrman

View original source ↗Export metadata ↓

Abstract

Pesticide concentrations in ground water at Rocky Mountain Arsenal (RMA) near Denver, Colorado, were determined using solid-phase extraction (SPE) gas chromatography/mass spectrometry (GC/MS) procedures and enzyme-linked immunosorbent assay (ELISA) for cyclodiene insecticides and triazine herbicides. Matrix interferences resulted in inconclusive results for some GC/MS analyses due to baseline disturbances and co-elution, but ELISA analyses consistently gave definitive results in a minimum amount of time. ELISA was used initially as a screening method, and pesticide concentrations and plume extents identified by ELISA were confirmed by SPE-GC/MS. A high degree of correlation was seen between results from GC/MS and ELISA methods for the triazine herbicides (correlation coefficient (R 2 ) = 0.99). All areas with high pesticide concentrations were found to be within the boundaries of RMA.

Explore related subjects

ACS Symposium Series Article

Source-reported bounding extent: 39.798428603322634° to 39.86995918074618° latitude; -104.90116833737858° to -104.79155154599407° longitude. This indicates report coverage, not an exact sampling location. View area on OpenStreetMap.

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

T. R. Dombrowski, E.M. Thurman, G. B. Mohrman. 2009-07-23. Evaluation of immunoassay for the determination of pesticides at a large-scale groundwater contamination site. https://doi.org/10.1021/bk-1997-0657.ch018

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related USGS reports

USGS2011

Redox chemistry and natural organic matter (NOM): Geochemists' dream, analytical chemists' nightmare

Natural organic matter (NOM) is an inherently complex mixture of polyfunctional organic molecules. Because of their universality and chemical reversibility, oxidation/reductions (redox) reactions of NOM have an especially interesting and important role in geochemistry. Variabilities in NOM composition and chemistry make studies of its redox chemistry particularly challenging, and details of NOM-mediated redox reactions are only partially understood. This is in large part due to the analytical difficulties associated with NOM characterization and the wide range of reagents and experimental systems used to study NOM redox reactions. This chapter provides a summary of the ongoing efforts to provide a coherent comprehension of aqueous redox chemistry involving NOM and of techniques for chemical characterization of NOM. It also describes some attempts to confirm the roles of different structural moieties in redox reactions. In addition, we discuss some of the operational parameters used to describe NOM redox capacities and redox states, and describe nomenclature of NOM redox chemistry. Several relatively facile experimental methods applicable to predictions of the NOM redox activity and redox states of NOM samples are discussed, with special attention to the proposed use of fluorescence spectroscopy to predict relevant redox characteristics of NOM samples.

ACS Symposium Series↗

USGS2010

A first application of enzyme-linked immunosorbent assay for screening cyclodiene insecticides in ground water

A commercially available enzyme-linked immunosorbent assay (ELISA) plate kit for screening of cyclodiene insecticides (aldrin, chlordane, dieldrin, endosulfan, endrin, and heptachlor) was evaluated for sensitivity, cross reactivity, and overall performance using ground-water samples from a contaminated site. Ground-water contaminants included several pesticide compounds and their manufacturing by-products, as well as many other organic and inorganic compounds. Cross-reactivity studies were carried out for the cyclodiene compounds, and results were compared to those listed by the manufacturer. Data obtained were used to evaluate the sensitivity of the ELISA kit to the cyclodiene compounds in ground water samples with a contaminated matrix. The method quantitation limit for the ELISA kit was 15 μg/L (as chlordane). Of the 56 ground-water samples analyzed using the ELISA plate kits, more than 85% showed cyclodiene insecticide contamination. The ELISA kit showed excellent potential as a screening tool for sites with suspected ground-water contamination by insecticides.

ACS Symposium Series↗

USGS2009

Herbicide metabolites in surface water and groundwater: Introduction and overview

Several future research topics for herbicide metabolites in surface and ground water are outlined in this chapter. They are herbicide usage, chemical analysis of metabolites, and fate and transport of metabolites in surface and ground water. These three ideas follow the themes in this book, which are the summary of a symposium of the American Chemical Society on herbicide metabolites in surface and ground water. First, geographic information systems allow the spatial distribution of herbicide-use data to be combined with geochemical information on fate and transport of herbicides. Next these two types of information are useful in predicting the kinds of metabolites present and their probable distribution in surface and ground water. Finally, methods development efforts may be focused on these specific target analytes. This chapter discusses these three concepts and provides an introduction to this book on the analysis, chemistry, and fate and transport of herbicide metabolites in surface and ground water.

ACS Symposium Series↗