USGS · 1014291
Control of whirling disease (Myxosoma cerebralis): Use of methylene blue staining as a possible indicator of effect of heat on spores
Abstract
Methylene blue staining (0.08 %) was used to determine efficiency of heat treatment in killing spores of Myxosoma cerebralis . Nearly all spores heated at 90°C for 10 min and 70°C for 100 min became stained giving presumptive evidence that they were killed.
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G. L. Hoffman, M.E. Markiw. 2006-01-24. Control of whirling disease (Myxosoma cerebralis): Use of methylene blue staining as a possible indicator of effect of heat on spores. https://doi.org/10.1111/j.1095-8649.1977.tb04051.x
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