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Source-linked reports with geographic coverage including United States.

At least 1,153 records · Page 64Linked to original sources

Rallus elegans (King Rail) occupancy is stable, but habitat is in short supply in the Arkansas Delta

Secretive marsh birds experienced precipitous declines due to losses in wetland habitat across North America. However, from 1998 to 2004, wetland area increased, and between 2009 and 2019, the extent of emergent wetlands did not significantly decrease. This raises the question: how did secretive marsh birds, which are tied to emergent wetlands, respond to this period of relative stability in wetland area? Here, we use Rallus elegans (King Rail) occurrence data collected in 2005, 2006, 2012, and 2017 in the Arkansas Delta in the United States to test the hypothesis that a period of stability in wetland vegetation is linked to stable Rallus elegans occupancy. Specifically, we sought to (1) quantify relationships between R. elegans occupancy and land cover, (2) quantify temporal trends in R. elegans occupancy, and (3) characterize changes in land cover types linked to R. elegans occupancy. We developed spatial multi-season occupancy models, and our top model contained the categorical year effect but showed no trend in R. elegans occupancy between 2005 and 2017, instead showing high interannual variation in occupancy. We found strong associations between R. elegans occupancy and emergent wetlands (positive) and elevation (negative). From 2005 to 2017 in the ∼3.7 million ha Arkansas Delta, rice crop cover decreased by 294,750 ha, emergent wetlands increased by 6,719 ha, and all other cover types increased or decreased by < 7,500 ha. Thus, although emergent wetlands did increase, the total area of emergent wetlands in 2016 (22,262 ha) comprised <1% of the Arkansas Delta. Our results support the hypothesis that R. elegans occupancy stabilized during a time when the main habitat type R. elegans depend on, emergent wetlands, also stabilized in spatial extent. There is an opportunity to turn the tide in R. elegans declines by conserving extant emergent wetlands and creating new emergent wetlands.

Arkansas

Does tidal marsh restoration lead to the recovery of trophic pathways that support estuarine fishes?

Evaluation of tidal marsh restoration success is typically based on the recovery of habitat size and target species. However, food-web structure may provide valuable insight into ecosystem functioning trajectories. Here, we studied restored tidal marshes of different ages ( new , young , old ; spanning 1–150 years) in comparison with nearby reference sites along the San Francisco Estuary. We asked: (1) How does restoration help recover energy pathways that support fishes? (2) Do fishes rely more on algal versus detrital pathways in restored sites?; and (3) How does food-web structure vary as a function of species origin and life history? To answer these questions, we sampled fish ( n = 806) and basal resources (emergent vegetation and phytoplankton; n = 109) seasonally over two hydrologically contrasting years. Using stable isotopes (δ 13 C, δ 15 N, and δ 34 S), we calculated fish isotopic niche volumes, food chain lengths, and the relative importance of algal versus detrital energy pathways. We found that food chains in restored sites were 8% shorter than in their paired reference sites. Additionally, the young and old restored sites had 37% smaller niche volumes than their references, but the opposite was true for the new restored site (11% larger), illustrating the characteristic trophic surge of early succession. Fishes found in restored sites relied significantly less on detrital energy (7% less) than fishes found in reference sites, and resident fishes showed 12% higher reliance on the detrital pathway than transient species. Finally, most of the native niche volume overlapped with that of introduced fish, which was in turn 38% larger, and a similar pattern was observed when comparing resident to transient fish. Our findings demonstrate that food-web structure does not immediately recover with tidal marsh restoration, even if fish assemblages are species-rich; and show that transient trophic surges may complicate restoration success assessments of newly restored marshes. We contend that incorporating recovery of energy pathways as an indicator of performance may help strengthen monitoring and design of wetland ecosystem restoration projects.

California

Ubiquity and causes of soil water preferential flow across 17 ecoregions

Preferential flow (PF) in soil causes the rapid transport of water, nutrients, and contaminants into the subsurface, influencing groundwater recharge and streamflow. Data scarcity has hindered the quantification of PF occurrence and the identification of its drivers across diverse ecoregions. We address this gap by analyzing high-frequency, multi-depth soil moisture data across 17 ecoregions in the USA, using ∼1,500 sensors at 40 sites. We discovered that PF is widespread, with sites experiencing PF in up to 60% of rainfall events ≥2 mm. Multiple approaches consistently show that PF is more likely to occur with increased peak rainfall intensity, finer textured material, low soil moisture variability, humid climate, and higher net primary productivity. This suggests that PF patterns could shift with projected climate changes, increasing uncertainty in predictions of groundwater recharge, water quality, and streamflow generation.

conterminous United States and Puerto Rico

Sapsucker wells as a keystone nutritional resource: Evaluating methods for detection of secondary sap consumers

North American sapsuckers are considered double keystone species because they (1) excavate nest cavities that are used by other birds, small mammals, and invertebrates, and (2) create and maintain sap wells, a temporary nutritional resource available to a variety of secondary consumers. Most previous reports of secondary sap consumption relied on human observers and were based on either brief or incidental observations. However, modern technology can greatly enhance observational techniques and provide additional insights into the functional, community-level importance of sap wells. We used visual surveys, camera traps, and environmental DNA (eDNA) to identify secondary consumers of sap from wells created by red-naped ( Sphyrapicus nuchalis ) and Williamson's ( S. thyroideus ) sapsuckers among three functional groups of shrubs and trees in south-central Colorado: shrub willows ( Salix spp.), Rocky Mountain maple ( Acer glabrum ), and conifer trees (Pinopsida). Camera traps and eDNA revealed additional sap-well visitors not identified from direct observations. Camera traps were effective for detecting nocturnal sap-well visitors such as small rodents as well as occasional diurnal visitors. Environmental DNA analyses corroborated findings from other methods and identified four additional taxa as possible sap consumers. The physiology of sap-well visitors, such as the ability to taste and assimilate compounds within sap, may aid in determining consumption versus contact when evaluating the results of eDNA analyses. Total vertebrate taxa detected using all 3 methods included 17 bird taxa in 10 families within 3 orders and 8 mammal taxa in 6 families within 4 orders. Shrub-willow sap wells attracted the most diverse vertebrate taxa (23), followed by Rocky Mountain maple (13) and conifer trees (10). Invertebrates in 13 families within 3 orders were observed feeding from sap wells during visual surveys. Because many secondary sap consumers perform ecological services such as pollination, seed dispersal, and pest control, the methods described here may aid in elucidating the importance of sap-well creators in supporting biodiversity and ecosystem functioning.

Colorado

Initial responses of songbird communities to forest reclamation on legacy surface mines

Surface coal mining and subsequent reclamation efforts in the Appalachian Mountains, USA, transform the ecological characteristics of natural landscapes. The Forestry Reclamation Approach (FRA) is a mine reclamation method that emphasizes best management practices in forestry. FRA practices have demonstrated success in establishing native forests and accelerating natural succession on coal mines; however, no studies have empirically examined the effects of the FRA on bird communities. Our study aimed to assess the avian community composition within young forests reclaimed using the FRA after one decade of forest growth. Whereas traditional reclamation practices often support grassland avian guilds, we expected that the FRA would provide habitat for shrubland and young forest avian guilds. Moreover, we sought to determine whether FRA forests would contain known avian indicator species of the native forest land cover. In June 2022, we conducted point count surveys in high-elevation, red spruce-northern hardwood (RS-NH) forests in the Appalachian Mountains of eastern West Virginia, USA. Using Bayesian multispecies occupancy models, we assessed avian guild occupancy and species richness within two FRA forest age classes (2–5 years and 8–11 years). We also examined avian community composition within two older RS-NH reference age classes to predict the future avian composition within FRA forests if reclamation succeeds. We found that the FRA breeding bird community included all of the avian indicator species expected to inhabit a young RS-NH forest. These results suggest that after approximately one decade, legacy mines reclaimed using the FRA are progressing toward a native RS-NH forest that supports associated forest bird communities.

West Virginia

Longevity, age-specific survival, and mean generation time of Rana muscosa: Implications for conservation of possibly the longest lived Ranid frog

Life history strategies vary widely among species and play a vital role in extinction risk, especially in a rapidly changing environment. For many taxa, information on life history such as longevity, lifespan, and generation time is incomplete. This is especially true for amphibians, which have experienced large-scale declines in recent decades. The mountain yellow-legged frog ( Rana muscosa ) is a California endemic recognized as a state and federally endangered species. We evaluated a 23-year dataset of six wild R. muscosa populations in southern California. We calculated the average lifespan of individuals in these six populations to be approximately 9.5 years, with a mean generation time of 7.4 years. We did not detect a difference in longevity between sexes or a difference in apparent survival across various ages of adults. We also documented the longest-lived ranid frog ever recorded from a wild population: a male R. muscosa that was at least 21 years old. Our results suggest a relatively long generation time for this species, a characteristic that may benefit them because reproduction is regularly challenged by drought, fire activity, and disease. This information is important for understanding the complex life history of this endangered ranid frog and can help guide efforts to manage and recover the species.

California

Sea-level driven isolation of glacial plant refugia revealed by submerged lake sediment from the Bering Land Bridge and St. Matthew Island

Bering Land Bridge (BLB) climate and vegetation during the Last Glacial Maximum (LGM) remains largely understudied, given challenges associated with collecting records from the submerged BLB. Previous records, confined to the margins of the modern land area and adjacent shelf, reveal conflicting interpretations of Beringian vegetation during the LGM. Here, we reconstruct LGM vegetation, sedimentology, and stable isotopes from a central BLB paleo-lake (Lake Knebel, LK) and compare it with a Holocene peat record from nearby St. Matthew Island (SMI). Results show strong similarities between LGM and late Holocene pollen assemblages, although with differences in relative taxonomic abundance. LGM communities are consistent with a cold and dry steppe or herb tundra environment but suggest the possibility of localized Betula presence in low-lying areas. LK’s bedded lacustrine stratigraphy transitions into undisturbed marine sediments by ~19 ka, providing a maximum limiting age of the transgression. Shrub absence on SMI today and during the Holocene is consistent with island isolation before ~14 to 15 ka, when Betula expanded rapidly at most sites with Bølling-Allerød warming. The combined vegetation evidence indicates preservation of LGM tundra and steppe vegetation assemblages on SMI, suggesting that island vegetation communities may provide additional constraints on the timing of sea level transgression.

Alaska

Tree swallows as indicators of per- and polyfluoroalkyl substance exposure and effects at select Department of Defense sites along the East Coast and at sites with different sources in the Upper Midwest, United States

Questions remain about the distribution of per- and polyfluoroalkyl substances (PFAS) in the environment, the sources and movement within and between ecosystems, and whether there are effects from such exposure. Information from the Upper Midwest and the mid-Atlantic regions of the United States, which have different PFAS sources, were investigated. Concentrations of Total 40 (sum of 40 PFAS), perfluorooctane sulfonate, perfluorohexane sulfonate, and Total 13 (sum of 13 PFAS) were consistently higher, by as much as a factor of 40, in tree swallow ( Tachycineta bicolor ) tissue samples (eggs, nestlings, and diet) at sites along the East Coast, where aqueous film-forming foams (AFFF) were extensively used when compared with East Coast reference sites. Sites in the Upper Midwest, with other PFAS sources, had qualitatively lower concentrations of PFAS than AFFF source sites. Perfluorooctane sulfonate was the only PFAS detected in all samples. Concentrations of most other PFAS, such as the carboxylates and fluorotelomers, did not differ between AFFF and reference sites. Perfluorohexane sulfonate, the second-most common constituent of some legacy AFFF formulations, was <1% of Total 40 at the reference sites in eggs and nestlings, but perfluorohexane sulfonate represented up to 9.7% (eggs) and 9.0% (nestlings) at AFFF-influenced sites. Despite differences in PFAS exposure, the daily probability of egg and nestling survival, as well as haptoglobin-like activity (PIT54) and total immunoglobulin Y, was similar across all sites. There were also no significant associations between these end points and concentrations of Total 40 or individual PFAS in eggs or nestlings.

East Coast, Upper Midwest

Postrelease movements of captive-reared adult Atlantic Salmon in two Maine rivers

Objective Atlantic Salmon Salmo salar remain at critically low levels in the United States, with the last remaining populations located in the state of Maine. In 2021, a pilot captive-rearing program, similar to a smolt-to-adult supplementation, was implemented to boost naturally spawning adults in support of recovery goals. Methods We conducted a 2-year acoustic telemetry study to track a subset of captive-reared salmon ( N = 270) that were released into the Penobscot and Machias rivers. We evaluated their postrelease movement patterns, dam passage, and site fidelity between the two rivers, years, and seasons. Results Atlantic Salmon that were released into the Penobscot River tended to overwinter, but their movement patterns varied between release years. In contrast, the Atlantic Salmon that were released into the Machias River were more likely to move directly downstream to the river exit. In addition, the fish that were released into the dammed Penobscot River frequently made multiple attempts to pass dams in both directions, often with passage delays and failures. Atlantic Salmon that were released during the summer displayed more exploratory movements than those that were released in the fall. Most Atlantic Salmon either left the river shortly after release (5–30 d) or the following spring (>120 d). Site fidelity was greater for the fall-released Atlantic Salmon (76%) than for the summer-released Atlantic Salmon (23%). Conclusions Overall, releasing salmon in the fall as sexually mature adults may increase site fidelity to the release reach, thereby enhancing the chances of successfully spawning in the wild.

Maine

Seasonal movements of nonnative White Catfish in the Penobscot River estuary

Objective White Catfish Ameiurus catus has been introduced to coastal watersheds across the United States. In the Penobscot River, Maine, this species has become increasingly common in upstream habitats that have been made accessible by recent dam removals. We characterized the movements of White Catfish to understand the temporal variation in their movement patterns and contextualize these findings within the recent changes in watershed connectivity. Methods We captured and tagged 10 adult White Catfish (mean fork length = 271 mm) with acoustic transmitters in the lower Penobscot River in July 2022. The movements of the tagged fish were monitored through April 2023 with a large network of stationary receivers. Results The tagged catfish were detected up to 6 km upstream and 31 km downstream from the release site. The total distance that was traveled by individuals ranged from 0 to 154 km during the study. Fall and spring movements were associated with changes in river flow and water temperature, but fish were relatively stationary from December through March, when at least five individuals were assumed to have overwintered in lower river tributaries. Conclusion Our results show that individual White Catfish may move considerable distances within large river systems and that these movements are potentially facilitated by changing river conditions. Collectively, this study fills a long-standing knowledge gap about the movement ecology of this species, adds context to help explain a recent increase in observations within their introduced range, and shows how changes in river conditions may be used to predict when and where these fish will move within a tidal system.

Maine

Genetic and environmental factors associated with survival of a rare songbird in a fragmented urban landscape

The coastal Cactus Wren ( Campylorhynchus brunneicapillus ) persists in small and fragmented populations throughout southern California that are subject to genetic drift and inbreeding. We combined individual banding and resighting data and genotyped individuals at 22 microsatellite loci to assess whether heterozygosity was associated with survival across three regional Cactus Wren populations on conserved lands in Orange and San Diego Counties between 2009 and 2020. Using Cormack-Jolly-Seber models (CJS) to analyze the 5-year capture histories of 528 individual wrens, we found that age class (hatch year or after hatch year) was the strongest predictor of survival. Individual heterozygosity and precipitation also had positive effects on survival, with survival up to 2 times higher in the most heterozygous individuals compared to the least and up to 1.5 times higher in high precipitation years versus drought years. Multi-locus heterozygosity was significantly correlated across loci, suggesting that inbreeding depression is likely driving the association between survival and heterozygosity. Study results support that genetic rescue efforts that reduce inbreeding have the potential to improve fitness and mitigate further loss of genetic variation in managed populations.

California

Experimental warming alters free-living nitrogen fixation in a humid tropical forest

Microbial nitrogen (N) fixation accounts for c. 97% of natural N inputs to terrestrial ecosystems. These microbes can be free-living in the soil and leaf litter (asymbiotic) or in symbiosis with plants. Warming is expected to increase N-fixation rates because warmer temperatures favor the growth and activity of N-fixing microbes. We investigated the effects of warming on asymbiotic components of N fixation at a field warming experiment in Puerto Rico. We analyzed the function and composition of bacterial communities from surface soil and leaf litter samples. Warming significantly increased asymbiotic N-fixation rates in soil by 55% (to 0.002 kg ha −1 yr −1 ) and by 525% in leaf litter (to 14.518 kg ha −1 yr −1 ) . This increase in N fixation was associated with changes in the N-fixing bacterial community composition and soil nutrients. Our findings suggest that warming increases the natural N inputs from the atmosphere into this tropical forest due to changes in microbial function and composition, especially in the leaf litter. Given the importance of leaf litter in nutrient cycling, future research should investigate other aspects of N cycles in the leaf litter under warming conditions.

Luquillo Experimental Forest, northeastern Puerto

SURF: An automated method for building nonplanar 3D fault models from earthquake hypocenters

Accurately characterizing 3D fault geometry is vital for improving our understanding of earthquake behavior and informing the development of seismic hazard models. Despite their importance, subsurface fault structures tend to be poorly constrained because of limitations in observational data. Improvements to the seismic networks and earthquake detection algorithms have increased the precision and volume of earthquake catalogs, which help illuminate detailed subsurface fault structure and provide the most direct information available about fault geometries at depth. We present a Python package to automate generating 3D fault geometries directly from hypocentral seismicity patterns. This method begins with clustering events based on their spatial density, identifying coherent patterns. Nearby clusters are then merged based on the similarity of their orientations. We fit nonplanar surfaces using support vector regression to balance surface accuracy with minimal deviations from planarity. The fault models are output as quadrilateral meshes at user‐defined resolution. In the process of generating the 3D fault surfaces, we compute the spatial density of seismicity around the surface and the planarity as quantitative metrics of the model outputs. As a proof of concept, we apply this approach to the San Andreas–Calaveras fault junction region and the 2019 Ridgecrest earthquake sequence, both in California, which contain complex subparallel faults well defined at the Earth’s surface and abundant microseismicity. These case studies demonstrate the method’s ability to model complex fault structures, including long continuous fault surfaces, crossing faults, variably dipping segments, and subparallel faults. We test the method on both standard network catalogs and double‐difference relocated catalogs. We find that our seismicity‐based fault model results align with published 3D models that incorporate additional constraints and interpretations ( Plesch et al. , 2020 ; Aagaard and Hirakawa, 2021 ). This workflow provides a low‐user‐input solution for estimating fault geometries at depth from earthquake catalogs.

California

Mapping a Carrington storm

A map is presented of median 1-min-resolution peak geoelectric-field strength across the United States as would be induced by magnetic storms as intense as the 2 September 1859 Carrington storm. The map is constructed from two data sets: Magnetometer time series from 22 ground-based observatories recording 40 magnetic storms, and surface impedance tensors derived from magnetotelluric measurements acquired at 1616 survey sites across the contiguous United States. Carrington-class storm geoelectric fields are likely to be very strong in the United States East and Midwest; > 5.00 V/km at many places. In Virginia, strengths would likely range from 30.30 V/km, with a 68% confidence interval of [19.44,47.20] V/km, to as low as 0.05 [0.03,0.07] V/km. Comparison of model geopotentials with those measured on 30 long lines, indicates errors of about 18%. A Carrington-class storm would likely induce geoelectric fields with strengths 55% greater than for the 13–14 March 1989 storm.

contiguous United States

Near real-time indicators of burn severity in the western U.S. from active fire tracking

Background Timely information on wildfire burn severity is critical to assess and mitigate potential post-fire impacts on soils, vegetation, and hillslope stability. Tracking individual fire spread and intensity using satellite active fire data provides a pathway to near real-time (NRT) information. Here, we generated a large database ( n = 2177) of wildfire events in the western United States (U.S.) between 2012 and 2021 using active fire detections from the Visible Infrared Imaging Radiometer Suite (VIIRS) sensor on the Suomi National Polar-orbiting Partnership (SNPP) satellite and the Fire Events Data Suite (FEDS) algorithm to track large fire growth every 12 h. We integrated fire tracking data with final fire perimeters and burn severity data from the Monitoring Trends in Burn Severity (MTBS) program to evaluate the relationship between burn severity and fire behavior metrics derived from the fire tracking approach, including the rate of fire spread and average fire radiative power (FRP) of fire detections for each 12-h growth increment. Results When stratified by vegetation type, FRP and rate of spread metrics were positively correlated with classified burn severity for each 12-h growth increment, highlighting the potential to rapidly identify areas of high and low severity burning. In forests, integrated measures of FRP over the fire lifetime captured persistent flaming and smoldering that compensated for initial differences between AM (01:30) and PM (13:30) fire detections. Predictive modeling of these relationships based on multiple fire behavior indicators and vegetation type from the LANDFIRE program yielded an accuracy of 78% for the separation of unburned/low and moderate/high burn severity classes. Conclusions These results demonstrate the ability to capture within-fire differences in burn severity using NRT indicators from fire tracking to assist with emergency management and disaster preparedness for post-fire hazards, such as landslides, debris flows, or changes in stream flow and water quality. As VIIRS data are available within minutes of each satellite overpass in the U.S., rapid estimates of burn severity based on fire tracking can be made days or weeks before a large wildfire is fully contained.

western United States

Submarine groundwater discharge creates cold‐water refugia that can mitigate exposure of heat stress in nearshore corals

Coral reef mortality around the world is accelerating due to human activities and rising sea temperatures that cause bleaching, which is expected to become more frequent. Our ability to predict which corals will be most resilient, however, remains limited due to insufficient information characterizing nearshore temperature and habitat conditions. In this study, we examine how submarine groundwater discharge (SGD) reduces nearshore water temperatures and exposure of corals to heat stress, complementing the understanding that SGD can adversely affect coral when it contains elevated nutrient concentrations. Data from fixed nearshore sensors and vertical depth profiles along ~100 km of the western shoreline of the Island of Hawai’i from 2003 to 2014 demonstrate that submarine groundwater discharge (SGD) can reduce nearshore water temperatures by 1 °C–5°C and create estuarine-like conditions with salinities as low as 20 PSU, where the prevalent coral species, Pocillopora meandrina, Porites lobata , and Montipora capitata , thrive. Time-series temperature records reveal that exposure to high ambient ocean temperatures, which are known to initiate bleaching events, are reduced up to 5%–46% of the time. Coral health surveys indicated coral bleaching in response to moderately high annual temperatures in 2010 and 2011, with more colonies affected farther from cold, SGD-fed waters. Synthesis of these results, along with coral response data following the more extreme marine heat wave of 2014–2015, demonstrates lower coral loss and greater coral recovery near groundwater seeps, particularly those with higher flux and influence on reducing nearshore water temperatures. Our results demonstrate that SGD may therefore provide a beneficial ecosystem service and enhance coral reef resilience, particularly where human-related nutrient additions to groundwater can be mitigated. The implications of our findings are relevant across tropical coasts where groundwater inputs can be substantial, such as the Caribbean and Indo-Pacific, and contribute to improving our understanding of coral sensitivity to gradients in temperature and nutrient stress. Improved management of groundwater resources could thus be vital to local–regional strategies for mitigating future heat stress.

Hawaii

Synthesis of observed field salinity ranges for oyster and seagrass species in the U.S.

Oyster and seagrass are important sessile, habitat-forming species that may be impacted by changes in salinity regimes from anthropogenic or climatic drivers. While salinity tolerance literature is focused on controlled experiments, observed field salinity ranges of species are more disparate. The salinity ranges in which organisms are observed in the field may not align exactly with their experimentally (wet lab) determined physiological salinity tolerances, since other critical factors control species occurrence. We performed a systematic review to synthesize observed practical salinity ranges for 10 oyster and 12 seagrass species in coastal waters of the United States. Of initially returned search results for oysters and seagrass, only 11.4% and 6.5% met our review inclusion criteria, respectively, corresponding to 3 oyster species and 5 seagrass species. The majority (95%) of the 57 reviewed studies were along the Atlantic and Gulf coasts, with 68% focusing on Crassostrea virginica . Shorter studies following episodic events were more common than long-term monitoring. Observed field salinity ranges were as follows (ppt): Crassostrea virginica (0.1 – 42.8, mean: 19.6), Ostrea lurida (1.7 – 32.5, mean: 27.8), Thalassia testudinum (0 – 65, mean 31.8), Halodule wrightii (<0.5 – 68.7, mean: 18.5), Syringodium filiforme (13.7 – 68.7, mean: 30.4), Ruppia maritima (0 – 29.4, mean: 3.8), and Zostera marina (19.7 – 51.2, mean: 28.9). Place-based examples highlight how reported overlapping salinity ranges for oyster and seagrass species can be used to expand existing habitat suitability models, inform restoration, and provide considerations for guiding holistic management of freshwater inflows to estuaries.

Estuaries and Coasts

Diel and spatial variability in cyanobacterial composition, gene abundance, and toxin concentration: A pilot study

We designed a pilot field study to assess relations between sunlight, cyanobacteria, and cyanotoxins. In 2021, we collected day (07:00 h, 10:00 h, 13:00 h, 16:00 h) and night samples (19:00 h, 22:00 h, 01:00 h, 04:00 h) at two locations in Kabetogama Lake, MN, USA. One sample set was collected from the lakeward end of a boat dock and the other on the nearby shoreline. Cyanobacterial phylogenetic eDNA differences over 24 h (pseudo F = 2.0938, p = 0.127) were not significant. Copies of anatoxin ( anaC ) and microcystin ( mcyE ) synthetase genes varied significantly over the sampling times at the dock (Friedman Χ 2 = 15.01, df = 7, p = 0.036; Friedman Χ 2 = 19.22, df = 7, p = 0.008) and the shoreline (Friedman Χ 2 = 19.33, df = 7, p = 0.007; Friedman Χ 2 = 20.56, df = 7, p = 0.005), with the highest anaC counts occurring during the night for both sites. Additionally, the highest total and dissolved microcystin concentrations occurred at night. Despite the proximity of the sampling locations, cyanobacterial phylogenetic eDNA results indicate that the variability between sites (pseudo-F = 27.547, p = 0.001) were greater than temporal differences over 24 h (pseudo F = 2.0938, p = 0.127). Understanding the effect of diel and spatial variability may help researchers and resource managers make informed decisions about sampling and potential exposure.

Minnesota