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Source-linked reports with geographic coverage including United States.

At least 937 records · Page 52Linked to original sources

Natural source zone depletion of crude oil in the subsurface: Processes controlling mass losses of individual compounds

At many petroleum hydrocarbon spill sites, residual spilled product forms a long-term source of groundwater contamination. The phrase source zone natural depletion is used to refer to the mass loss rates. Overall mass lost under environmental conditions was analyzed using conservative biomarker concentrations for a 1979 oil spill in northern Minnesota, USA. After 40–41 years, an average of 50% of the mass was lost with values ranging from 22% to 57% depending on location. It is also important to understand the composition changes in the source. To understand controls on the losses of individual compounds, concentrations of volatile hydrocarbons in oil samples were compared with aqueous solubilities, and pore-space oil saturations. The results of the comparison show that losses of the oil compounds were controlled by pore-space oil saturations, solubility, and susceptibility to degradation under methanogenic conditions. Compounds that degrade under methanogenic conditions, including toluene, o -xylene, and n -alkanes are more depleted compared to benzene, ethylbenzene, and m - and p -xylene for which losses are dominated by dissolution. These rates and compound-specific behaviors form a foundation for improved modeling approaches and risk analyses.

Minnesota

Moose survival and habitat‐associated risk of endoparasites

Parasite-induced morbidity and mortality can alter the trajectories of incidental host populations. Yet, parasites rarely act in isolation and may be one of a multitude of biotic and abiotic stressors that collectively shape mortality risk in vertebrate populations. We quantified sources of mortality in a low-density population of moose ( Alces alces ) in New York State and investigated factors including parasite infection, nutritional limitation, and thermal stress influencing mortality risk in calf moose. We observed high rates of annual survival (0.81–0.92) in adult ( n = 25) and calf ( n = 27) moose monitored 2015–2018 and 2022–2024, respectively. The majority of cause-specific mortality was attributed to disease induced by giant liver fluke ( Fascioloides magna ; 75% in adults, 67% in calves). Calf mortality risk increased by 72% for every unit increase in giant liver fluke infection risk, measured as cumulative monthly proportion of wetlands used by moose, and decreased by 16% with each additional unit of nutritional energy available. The combination of flukes, coinfecting parasites, and available nutritional energy is important to calf survival in this population, highlighting the importance of managing multiple stressors for species conservation, although the effects are hard to disentangle given the high rates of survival observed. Identifying causes of mortality and mechanisms underlying increased mortality risk contributes to the continued conservation of moose in fluctuating populations and highlights the importance of managing parasite-induced disease.

New York

Landowners' cognitions and motivations coupled with practice durability influence persistence in grazing agricultural conservation practices in southwest Virginia

Agricultural conservation practices are often used to protect stream health while continuing food production. However, recovery of stream health is often not as rapid or extensive as planned. The efficacy of practices may be improved by promoting their continued use by landowners (i.e., persistence) after cost-share contracts with government agencies end. Persistence rates and their drivers are typically unknown. Therefore, we mail-surveyed 889 landowners to understand persistence in grazing practices in southwest Virginia. Survey responses were analyzed quantitatively using logistic regression and qualitatively via coding. We found that persistence rates for vegetative practices, cattle-exclusion fencing, off-stream watering structures, and pasture management were 74%, 84%, 94%, and 94%, respectively. Both the quantitative and qualitative analyses indicated that landowner cognitions (i.e., attitudes toward practices, environment, and agencies), environmental motivations, and practice durability influence persistence. Our results highlight that persistence could be encouraged by: (1) providing targeted messaging that demonstrates persistence benefits and aligns with landowners' motivations, (2) ensuring that responsibility to maintain practices is transferred during land-tenure changes, and (3) allocating more agency funding to practice maintenance.

Virginia

Assessment of treated wastewater infiltration in Bright Angel Wash and the potential for contaminants of emerging concern influencing spring water quality along the South Rim of the Grand Canyon in Grand Canyon National Park, Arizona

In April 2021, a synoptic study conducted by the U.S. Geological Survey (USGS) and National Park Service (NPS) identified wastewater-related contaminants of emerging concern (CECs) in springs along the South Rim of the Grand Canyon. These springs are located north of Bright Angel Wash, an ephemeral channel that receives treated effluent from the South Rim Wastewater Treatment Plant (SRWTP). Although water flows southwest and away from the canyon, there is evidence that treated wastewater is finding a flow path along fractures associated with the Bright Angel Fault back to water sources along the South Rim. The CECs identified during the April 2021 sampling included several per- and polyfluoroalkyl substances (PFAS) and pharmaceutical compounds. The PFAS compounds detected only consisted of perfluoroalkyl acids, and these were only detected at Bright Angel Wash (treated wastewater), Monument Spring, and upper Horn Bedrock Spring. The other five sampled springs (the Salt Creek, Horn East Alluvium, Garden, Pumphouse, and Pipe Springs) had no detections of PFAS compounds. The five perfluoroalkyl acids detected at Monument Spring (in descending order of concentration) were perfluorobutanesulfonic acid (PFBS), perfluoropentanoic acid, perfluorooctanoic acid, perfluorohexanoic acid, and perfluorooctanesulfonic acid. Only the short-chained PFBS and perfluorobutanoic acid compounds were detected at the upper Horn Bedrock Spring. All the same perfluoroalkyl acids were found at Bright Angel Wash, except for PFBS. Monument Spring was the only spring to have detections of pharmaceuticals. The two pharmaceuticals detected at the highest concentrations at Monument Spring were diphenhydramine (antihistamine) and carbamazepine (anticonvulsant or anti-epileptic drug). The other detected pharmaceuticals included (in descending order of concentration) fluconazole (antifungal), sulfamethoxazole (antibiotic), metformin (diabetes drug), tramadol (opioid analgesic), and venlafaxine (antidepressant and nerve pain medication). The same pharmaceuticals were detected in the wastewater at Bright Angel Wash but in greater concentrations. No CEC concentrations measured at Monument Spring exceeded any drinking water standards or human health benchmarks; however, most of the compounds detected have no regulatory standards. Studies of the ecological effects of these compounds show that some of the compounds detected can have endocrine and physiological effects, but generally, effects were observed at concentrations multiple orders of magnitude greater than what was measured during the April 2021 study. Data from 1980 through 2022 retrieved from the Water Quality Portal were combined with data from the one-time synoptic sampling in April 2021 to assess the usefulness of other analytes for identifying a wastewater connection to South Rim springs. Most of the historical water chemistry data showed a statistical difference between samples collected within and east of the Garden Creek watershed and samples collected from locations in watersheds to the west of Garden Creek, including the Horn Creek, Salt Creek, Monument Creek, and Hermit Creek watersheds, which roughly align with the Bright Angel Fault. Most of the historical analytes were inconclusive as potential wastewater tracers, but nitrate, chloride, and gadolinium data possibly support the historical contribution of wastewater to Monument Spring.

Arizona

Pleistocene porcupine (Erethizontidae) records in arid southwestern North America and comparisons with the modern distribution of Erethizon dorsatum in southern California and Arizona

he North American porcupine ( Erethizon dorsatum ) is widely distributed throughout many ecosystems on the continent from northern Mexico to the Arctic Ocean, except for the southeastern portion of the United States. Habi­tats include the arid Desert Southwest region where modern records are generally sparse. The paleogeographic range in the Desert Southwest is not unlike the modern distribution of Erethizon , with some exceptions. Although there are early Pleistocene records of Erethizon for southern California, no late Pleistocene records (Rancholabrean North American Land Mammal Age [NALMA]) are known for Imperial, Inyo, Riverside, or San Bernardino Counties in southern California or in Mexico. This is surprising given late-Pleistocene records in Arizona, Nevada, and New Mexico and modern records from southern California and Mexico. Pleistocene habitats for porcupines were similar to those occupied today, just at different elevations due to differing climates. Modern preferred habitats in the Desert Southwest include conifer-clad high-elevation areas and associated pinyon pine / juniper belts, and occasionally riparian corridors in desert environments. The overall rarity of porcupines today in arid parts of the Desert Southwest is likely due to the combination of increasing aridity in the region during the Holocene, past persecution by humans, and increasing predator populations.

Arizona, California

Watershed forest cover and habitat restoration can offset some negative impacts of climate change on freshwater fishes and mussels

Many species of freshwater fishes and freshwater mussels have experienced population declines over the past century due to threats including habitat degradation, overexploitation, species invasion, and climate change. Management actions may offset climate-related changes to biodiversity, although identifying appropriate strategies is challenging. Our goal was to identify the impacts of climate change on freshwater biota (i.e., fish and mussel) distribution and management actions that may offset the climate change impacts across the northeastern United States. We used land use, geography, stream temperature, and streamflow variables to predict species distribution in a baseline scenario, climate change scenario, and several climate change plus land use management scenarios. We found climate change negatively impacted (i.e., reduced the probability of occurrence of) coldwater fishes and reduced the relative occurrence probability of fluvial specialist and coolwater fishes compared to other species. Increasing watershed forest cover best offset these negative impacts and minimized the predicted transition from coldwater fish dominance to warmwater fish dominance in coldwater habitats; however, no intervention fully offset the negative impacts of climate change on vulnerable fish groups (i.e., coldwater and fluvial specialist fishes). Climate change negatively impacted all vulnerable groups of mussels (e.g., lotic species, drying intolerant) and mussel species richness. Combining multiple management interventions (e.g., increase forest cover, dam removal, etc.) had the greatest potential to offset the negative impacts of climate change for freshwater mussels and fishes. This study provides managers a comparison of management interventions across a landscape to combat the impacts of climate change on biota in streams and rivers.

Connecticut, Maine, Massachusetts, New Hampshire,

Ecovoltaic solar energy development effects to microclimate, temperature, and soil moisture in panel array interspaces in a warm desert

Solar energy development is increasing in warm deserts of the southwestern United States, and ecovoltaics has emerged as an approach to maintain ecosystem function within solar facilities while meeting increasing regional energy demands. The Solar Gemini Project, located in the northeastern Mojave Desert, USA, is one of largest photovoltaic facilities incorporating an ecovoltaics approach, including novel installation practices to reduce disturbance to soil and vegetation and solar tracking bifacial photovoltaic panels to maximize energy generation. To further understand the influence of the facility on environmental conditions, we compared microclimate, temperature, and soil moisture in bare soil interspaces between panel arrays inside the facility to undisturbed desert in nearby areas outside the facility from June 2023–February 2025. Our comparisons included different seasons (summer versus winter), soil depth (shallow versus deep), and facility operation (inactive versus active panels with solar-tracking). We found lower solar radiation, wind speed, and evaporative demand inside the facility compared to outside the facility, with greater differences when panels were actively tracking. When panels were active, air temperature inside and outside the facility was similar on average, but was higher inside the facility during the day and lower at night. Soil surface temperature was lower inside the facility due to panel shading in the morning and evening, whereas soil temperature (0–15 cm) was consistently higher inside the facility. Soil moisture was higher inside the facility and did not drop to the low levels observed outside the facility. In total, we found lower evaporative demand, less heat loading, and higher soil moisture inside the Gemini facility, potentially benefitting flora and fauna in the harsh desert landscape. Yet, we also found daytime increases in air and soil surface temperature inside the facility, and persistent increases to soil temperature. These findings highlight the potentially positive and negative environmental changes associated with ecovoltaics solar energy development in warm deserts and provide evidence that can inform the optimization of solar photovoltaics design and management.

Nevada

Potential interactions between birds and floating photovoltaic solar energy: Spatially informed species vulnerabilities, techno-ecological risks, and sustainability trade-offs

Floating photovoltaics (floating solar panels; FPV) can reduce the negative impacts of solar energy development in terrestrial environments, but their effects on freshwater ecosystems remain poorly understood. We examined potential FPV interactions with avian biodiversity, using previously modeled technical potential of FPV in the northeastern United States. We developed a vulnerability index based on avian species’: (i) morphological traits, (ii) level of conservation concern, and (iii) habitat requirements. Using eBird participatory science data, we assessed the risk of FPV to 291 species of birds occurring at FPV-suitable waterbodies based on their vulnerability index and estimated relative abundance at each location. The greatest risk of FPVs to birds was associated with waterbodies near the Atlantic coast and Great Lakes, and most (80%) of these high-risk waterbodies were smaller waterbodies. Because the avian risk at FPV-suitable waterbodies was not correlated with estimated energy generation, reducing risk to birds might not require energy trade-offs. Moreover, avoiding FPV installations at waterbodies predicted to pose high risk to birds is likely to return social and recreational benefits while minimizing risk of biofouling. Our analysis provides a framework for proactively evaluating risk, reducing potential adverse impacts of FPV to birds, and identifying cobenefits of sustainable FPV siting across waterscapes.

Connecticut, Delaware, Maine, Maryland, Massachuse

The impact of 3D structure on coseismic coastal land-level change and tsunami generation in the Cascadia Subduction Zone

Estimates of coseismic vertical displacements from past and potential future subduction zone earthquakes provide critical constraints on regional seismic and tsunami hazard. Many studies use elastic homogeneous half-space models to calculate vertical displacements from a specified earthquake slip distribution, neglecting complexities of 3D structure in subduction zone settings. Here, we use 3D ground motion simulations of potential Cascadia Subduction Zone earthquakes to investigate the impact of realistic 3D Earth structure on estimated vertical displacements. We show that offshore uplift increases when including 3D structure due to high fault slip in low-rigidity accretionary wedge sediments and coastal subsidence decreases due to higher rigidity material at depth. These larger offshore uplifts cause increased tsunami maximum wave heights, and coastal subsidence at paleoseismic sites decreases on average by ∼17 cm, or ∼60%, which can have important implications for the amplitude and extent of slip in earthquake scenarios developed based on paleoseismic data constraints.

California, Oregon, Washington

Using visualization science to inform the design of environmental decision-support tools—A case study of the U.S. Geological Survey Waterwatch

Environmental decision-support tools are increasingly being used to serve both expert and non-expert audiences. Many existing tools are primarily expert-focused, and redesigning them can be challenging because experts and non-experts interact with tools differently, existing users may be resistant to changes, and there is little guidance on how to prioritize redesign efforts and demonstrate their efficacy. In this report, we present a case study of a user-centered redesign of an established environmental decision-support tool—the U.S. Geological Survey WaterWatch. WaterWatch supports flood, drought, and other water resource management decisions through the display of water levels at gages across the United States. Using a participatory process, we identified a functional change (replacing the existing rainbow colormap), created an alternative design, and tested the alternative’s usability through two general public surveys. The results showed that replacing the rainbow colormap with a more intuitive diverging colormap improves usability, regardless of the audience’s subjective preference for the rainbow color scheme. In addition, we demonstrated the importance of using legends to improve the audience’s understanding of the map symbols. This study demonstrates how user-centered design approaches can be used to inform the design of high-profile products and tools.

Scientific Investigations Report

A 10-year continuous daily simulation of chloride flux from a suburban watershed in Fairfax County, Virginia, USA

Increasing levels of chloride in surface water are associated with detrimental effects on water quality, aquatic ecosystems, infrastructure, and human health. Numerous mass-balance studies have inferred watershed transport processes by interpreting chloride inputs and outputs, but few represent internal dynamics explicitly. We constructed a coupled water/chloride mass balance model to gain insights into storage, residence time, and transport processes in a 10-km 2 urban watershed. The model, which operates over a 10-year period at a daily time scale, represents storage in a dynamic soil-moisture reservoir, quick-flow runoff from storm events, and slow-flow runoff that sustains streamflow in dry weather. The calibrated model accurately represented (a)the observed transition from a streamflow enrichment regime in cold months to a dilution regime in warmer months, (b) the observed tendency for late-summer concentrations to be higher after winters with heavy snowfall, and (c) a period-of-record downward trend in chloride concentration likely associated with a downward trend in annual snowfall. Estimated chloride inputs averaged 195 metric tons per year, while the average output was 270 metric tons per year. In contrast, estimated storage was only 107 metric tons. The estimated mean residence time in groundwater was 1.27 years. This short residence time indicates that efforts to reduce inputs will manifest as decreased concentrations in streamflow on a management-relevant time scale of several years. The coupled mass balance model yielded insights into internal watershed dynamics that would not be possible from simple input/output analysis; such models can be useful tools for gaining insight into small watershed hydrology and pollutant transport.

Virginia

The value of reducing uncertainties to support the management of a high‐elevation endemic salamander

Many salamander populations are declining, and methods to determine how best to allocate limited resources to slow or reverse these declines could support land managers in their decision‐making processes. Multiple types of uncertainty may delay management decisions, including when (1) knowledge of a species' ecology is incomplete, (2) climate change effects on environmental covariates are uncertain, and (3) the efficacy of management alternatives is unknown. For management decisions, a value‐of‐information analysis can identify which uncertainties are critical to reduce in order to identify an optimal strategy from a set of possible management actions. If the same management action is optimal across the full range of uncertainties, then resources for research can be redirected toward active management. Using value‐of‐information analyses, we examine the effect of uncertainty on identifying optimal management to maximize the future expected occupancy of Plethodon shenandoah , a Federally Endangered high‐elevation endemic salamander that is threatened by climate change. Out of 11 management actions proposed by National Park Service managers, those that increase environmental moisture are expected to maximize occupancy, and we find that the selection of this action is robust to all the identified uncertainties. We show that, even in systems with multiple sources of large uncertainty, value of information analyses discriminate among investments in species management.

Ecosphere

Big River bluegill: Combining vital rates and long-term monitoring to understand population dynamics in large rivers

Long-term monitoring indicates bluegill catch rates are relatively stable in some reaches of the Upper Mississippi River and highly variable in others, whereas in the Illinois River, catch rates have decreased. A lack of age structure information precludes understanding population processes responsible for patterns in catch rates. To build a better understanding of why catch rates have changed over time, we integrated short-term age structure information with long-term monitoring data to quantify and assess spatial patterns in bluegill population dynamics across six study reaches of these two rivers. Specifically, we estimated and compared age and size structure, growth, maturity, mortality, and recruitment. We used quantile regressions to apply age estimates to long-term data for investigating trends in age-based catch rates reflective of recruitment (age-1 catch rates), mortality (using age-1 and age-2+ catch rates), and spawning stock (age-2+). Our findings indicate trends in bluegill age-2+ catch rates increased and then stabilized across upstream study reaches, but dynamic rates, size structure, and age at maturity varied among reaches. Bluegill populations in downstream study reaches had low maximum size, early maturation, low mean age, low proportional stock density, declining recruitment, and declining age-2+ catch rates. An insufficient number of bluegills were collected from the study reach furthest downstream to adequately quantify dynamic rates. Our results support life history theory in that bluegill respond to unstable environmental conditions through life history adaptations. These findings show how integrating periodic age structure information with long-term monitoring can enhance population assessments.

Illinois, Iowa, Minnesota, Missouri, Wisconsin

Evaluating uncertainties with sample-collection method and source selection in sediment fingerprinting: an example from a Great Lakes tributary

Purpose Identifying suspended-sediment (SS) sources, seasonal variability, and phosphorus (P) transported with SS is critical information for basin managers, although there may be concerns about comparability between flow-integrated SS samples used for sediment fingerprinting and discrete samples used for concentrations and loads in basins where SS is mostly silt + clay and(or) one land-use predominates. Objectives were to determine if (1) sample-collection method and (2) source consideration influenced apportionment of the largest SS source. Methods Concurrent-replicate, SS samples were collected during 2022 from the East River, Wisconsin using an automated sampler, commonly used for water-quality sampling, and passive samplers, frequently used for SS fingerprinting. Samples were evaluated for differences in physical and chemical characteristics that may affect source apportionment. Considered sources included three upland land-use (cropland, forest, and roads), two in-channel (streambank and streambed sediment), and one that connects uplands to the stream channel (gullies). Source apportionment used established methods in the SedSAT tool. Source scenarios included land-use + streambank (4src), 4src + gully, 4src + streambed, and 4src + gully + streambed (6src). Results There were no statistically significant differences in median grain size, organic carbon, or sediment-bound P as a function of collection method. In-channel sources were the largest proportional SS source, regardless of season, hydrologic condition, collection method, or source scenario. Source verification highlighted which source fingerprints were most accurately defined and implications for SS target apportionment. Conclusion Varying the source scenarios for sediment fingerprinting indicated that improved management of hydrologic connectivity between upland land use and the stream channel has the potential to mitigate SS loads.

Wisconsin

Assessing environmental drivers and protist community dynamics that shaped the historic August 2022 Heterosigma akashiwo bloom in San Francisco Bay, California

San Francisco Bay, California, typically has chlorophyll a (chl- a ) concentrations below 10 µg L −1 , despite nutrient loadings exceeding those in many estuaries with recurring harmful algal blooms (HABs). However, in August 2022 there was a Heterosigma akashiwo (raphidophyte) bloom with chl- a concentrations exceeding 450 µg L −1 , resulting in widespread hypoxia and fish die-off. We used protist community (18S ribosomal ribonucleic acid gene metabarcoding) and environmental data collected 2–3 times per month from 2015–2023 to assess differences between 2022 and other years, and capture dynamics pre-, during, and post-bloom. The 2022 protist community clustered separately from other years in non-metric multidimensional scaling and hierarchical cluster analyses. A diatom bloom in June generated the highest median June chl- a concentration (15 µg L −1 ) recorded between 1977–2023 and drew down nutrients (nitrogen, silicate), leading to the establishment of a heterotrophic community in July with low dinoflagellate abundance (0.9% of protists versus 5.9% average in other years). The absence of dinoflagellates, potentially including H. akashiwo predators , plus suitable habitat (13–14 daylight hours, temperatures 17–21˚C, nitrate concentrations 17–24 µmol L −1 , neap tides) likely provided opportunity for H. akashiwo to proliferate from 0.03% to 48% of protists between July 20 and August 25. As the bloom collapsed, the genus Gyrodinium (dinoflagellate predator of H. akashiwo ) increased from 3.0% to 52% of protists between August 25 and August 31, likely exerting grazing pressure. Results show that antecedent protist community dynamics play important roles in shaping HABs, and interactions between biotic and abiotic factors govern the development of blooms.

California

How do hydrological variability and human activities control the spatiotemporal changes of riverine nitrogen export in the Upper Mississippi River Basin?

Excessive nitrogen export from agricultural watersheds remains a critical water quality challenge, with the Upper Mississippi River Basin (UMRB) significantly contributing to downstream eutrophication and hypoxia in the Gulf. This study investigates the spatiotemporal dynamics of riverine nitrate plus nitrite (NO 3 – + NO 2 – -N) export across the UMRB at high spatial resolution (12-digit Hydrologic Unit Codes or HUC12 subwatershed scale) during 2001–2020 and quantifies the effects of anthropogenic activities and hydrological variability on riverine NO 3 – + NO 2 – -N export changes in the region between 2001–2005 and 2016–2020. Our results revealed hotspots of substantial increases in NO 3 – + NO 2 – -N yields across the UMRB, with distinct regional patterns in driving factors. Over the entire UMRB, NO 3 – + NO 2 – -N yields increased by 9.7 kg/ha/yr on average from 2001–2005 to 2016–2020, with anthropogenic activities contributing 4.8 kg/ha/yr and hydrological variability contributing 4.9 kg/ha/yr. The northern and western UMRB had combined influences from both anthropogenic activities and hydrological variability, while the east-central regions had predominantly hydrologically driven changes. Agricultural sources, including fertilizer, manure, and biological nitrogen fixation, collectively contributed over 80% of NO 3 – + NO 2 – -N loading throughout the basin. This framework for disentangling human and hydrological impacts provides critical insights for developing effective and targeted watershed management strategies to reduce nutrient losses and improve water quality.

Upper Mississippi River Basin

Insights into widespread landsliding in southern Appalachia from Hurricane Helene

Between 23 and 27 September 2024, antecedent rain followed by Hurricane Helene produced one of the most damaging weather events in southern Appalachia history. The back-to-back storm events resulted in a maximum cumulative rainfall of 848 mm and hurricane-force wind gusts over 170 km/h in western North Carolina, eastern Tennessee, and southwestern Virginia. The resulting regional flooding, landslides, and tree blowdown caused over 100 fatalities, damaged or destroyed critical infrastructure and thousands of structures, and severed connectivity across the region. Over the next several weeks, a multi-agency landslide response produced a rapid hazard assessment and mapped 2217 landslides, 55% of which damaged infrastructure or property. Orographic uplift enhanced rainfall, resulting in concentrated landsliding along the ~250 km swath of the Blue Ridge escarpment in western North Carolina. Landslides initiated predominantly on windward-facing (southeast-facing) slopes, and localized clustering of initiation points indicated a strong influence of hillslope-scale meteorological and geomorphic factors. Many shallow landslides mobilized into larger, highly mobile, and damaging debris flows that graded into floods. Here, we put our preliminary observations in the context of historical storm-driven landslide events and open new avenues for investigating the nature and extent of landslides and their effects in southern Appalachia and similar environments.

Georgia, North Carolina, South Carolina, Tennessee