Geology topics

Research about Montana, Wyoming

Source-linked reports with geographic coverage including Montana, Wyoming.

At least 19 recordsLinked to original sources

USGS2026

Mid-Cretaceous porphyritic magmatism in the Beartooth Mountains of the northern Laramide foreland and its connection to Frontier Formation sediment dispersal in Bighorn basin, Montana-Wyoming (USA), and Cordilleran tectonism

Porphyritic intrusions in the southeast Beartooth Mountains and porphyritic cobbles from the Torchlight conglomerate of the Cenomanian Frontier Formation in Bighorn basin (Montana-Wyoming, USA) bear striking resemblance. We utilize geologic mapping, petrography, geochemistry, and geochronology to characterize rocks from both locations and test whether the Beartooth Mountains area was the provenance for Bighorn basin cobbles. Mapping on Line Creek Plateau in the Beartooth Mountains shows three units with porphyritic phenocrysts consisting dominantly of plagioclase, plagioclase + potassium feldspar, and plagioclase + quartz. Petrography shows embayed quartz, opacitic rims, and zoned plagioclase. Geochemistry characterizes the intrusions as trachydacite to trachyte and trace elements exhibit enrichment in light rare earth elements and slight depletion in heavy rare earth elements relative to primitive mantle. Zircon U-Pb dating shows substantial Archean inheritance and ca. 104−92 Ma dates; some samples display multiple mid-Cretaceous date populations. Bighorn basin porphyritic cobbles exhibit similar modal mineralogy, near-identical geochemistry, and Archean inheritance and mid-Cretaceous geochronology. We suggest the porphyritic cobbles were sourced from basement-hosted porphyritic intrusions now exposed in the Beartooth Mountains, given that geochemistry does not agree with coeval volcanic sources along the Frontier Formation depositional fairway (Idaho batholith) or sedimentary-hosted Beartooth Mountains porphyritic sills intruded into Cambrian shale. Stratigraphic evidence does not support kilometer-scale mid-Cretaceous Beartooth Mountains exhumation to expose basement-hosted porphyritic intrusions, and we therefore present a volcanic hypothesis to bring rocks to the surface. This mid-Cretaceous multimillion-year magmatism is unexplained by tectonic models for the north-central Laramide foreland, and thus we further explore the emplacement processes, spatiotemporal significance, and tectonic implications of these rocks.

Montana, Wyoming↗

USGS2026

Snow simulations predict future changes in rain-on-snow events across the upper Gallatin River watershed, a Greater Yellowstone Ecosystem headwater system

Study region: The upper Gallatin River watershed, an alpine headwater system in the Greater Yellowstone Ecosystem, in Wyoming and Montana. Study focus: As global and regional air temperatures rise, mountain headwaters across the Greater Yellowstone Ecosystem (GYE) are projected to see more precipitation falling as rain. While the hydrologic effects of this snow-to-rain transition depends on a variety of factors, it can lead to an increased occurrence of rain-on-snow (RoS) events. To investigate these changes, we used high-resolution (30 m) SnowModel simulations of the upper Gallatin River watershed. Simulations were run for 2001-2013 using two scenarios: (1) historical meteorology as control and (2) pseudo global warming (PGW) where control air temperature and precipitation conditions were perturbed to represent mean end-of-century conditions under a high-emissions scenario. New hydrological insights for the region: SnowModel outputs show that changes in PGW precipitation and snow accumulation varied with elevation. Warmer air temperatures at low elevations (< 2,500 m) led to less snow accumulation and less precipitation falling as snow. Colder baseline air temperatures for elevations above 2,500 meters (m) resulted in minor reductions in winter snowfall fraction. For PGW simulations, spring (April-June) months were rainier, and elevations above 2,500 m experienced more RoS events. Snowpacks between 2,500-3,100 m generated more snowmelt during RoS events, which was reflected in the watershed average. More high-intensity melt events can affect aquatic habitat, water quality, and the accuracy of streamflow forecasts across the region.

Montana, Wyoming↗

USGS2025

Female and male grizzly bears differ in their responses to low-intensity recreation in a protected area

Strategies animals use to navigate human-dominated landscapes frequently mimic anti-predator responses employed by prey species. Understanding how large carnivores respond to outdoor recreation is important for conservation, particularly in protected areas with preservation mandates. Visitation to Yellowstone National Park doubled from 1980 to 2015, increasing the need to examine potential changes in behavior of grizzly bears ( Ursus arctos ) in relation to human recreation sites (trails, backcountry campsites). We developed integrated step-selection functions to explore how recreation sites influenced the movement rate and selection by male and female grizzly bears. Further, we tested whether time of day (diurnal, crepuscular, nocturnal) and restrictions to human access (i.e., restricted, unrestricted) modified bear responses and then compared behaviors based on proximity to recreation sites. Male grizzly bears used trails to travel during crepuscular and nocturnal hours and exhibited more pronounced behavior in restricted areas compared with unrestricted areas, suggesting recreation in unrestricted areas influenced the behavior of male bears. In contrast, female bears varied their movement rate and selection of trails in restricted areas much more than in unrestricted areas, suggesting females may make security tradeoffs between male bears and people. Both sexes used trails, likely as energetically efficient travel corridors; however, our analyses did not indicate that bears spent time near backcountry campsites. The sex-based differences in selection and movement patterns associated with trails and campsites suggest a single management approach for recreation may not equally benefit all bears. Recreation impacts on wildlife are complex to characterize and predict, but simultaneously modeling movement and selection provides a more comprehensive assessment of strategies animals use to navigate perceived risk.

Montana, Wyoming↗

USGS2025

Peak streamflow trends in Montana and northern Wyoming and their relation to changes in climate, water years 1921–2020

Frequency analysis on annual peak streamflow (hereinafter, peak flow) is essential to water-resources management applications, including critical structure design (for example, bridges and culverts) and floodplain mapping. Nonstationarity is a statistical property of a peak-flow series such that the distributional properties (the mean, variance, or skew) change either gradually (monotonic trend) or abruptly (shift, step change or change point) through time. Not incorporating or accounting for observed nonstationarity into peak-flow frequency analysis might result in a poor representation of the true probability of large floods and thus misrepresent the actual flood risks to life and property. This report summarizes how hydroclimatic variability might affect the temporal and spatial distributions of peak-flow data in the State of Montana (and northern Wyoming) and is part of a larger study to document peak-flow nonstationarity and hydroclimatic changes across a nine-State region consisting of Illinois, Iowa, Michigan, Minnesota, Missouri, Montana, North Dakota, South Dakota, and Wisconsin. A wide range of analyses and statistical approaches are applied to document the primary mechanisms controlling floods and characterize temporal changes in hydroclimatic variables and peak flows. This study was completed in cooperation with the Montana Department of Natural Resources and Conservation. The purpose of this report is to characterize temporal and spatial patterns of nonstationarity in peak flows and hydroclimatology in Montana and northern Wyoming. In this evaluation, peak-flow, daily streamflow, and model-simulated gridded climatic data were examined for monotonic trends, change points, and other statistical properties that might indicate changing climatic and environmental conditions. This report includes background information on the study area, the history of U.S. Geological Survey peak-flow data collection and frequency analysis in Montana, and the review of research relating to hydroclimatic variability and change in Montana. This study might help provide a framework for addressing potential nonstationarity issues in peak-flow frequency updates that commonly are completed by the U.S. Geological Survey in cooperation with other agencies throughout the Nation. The analytical structure of this study includes analyses of monotonic trends and change points in numerous hydroclimatic variables in assigned 30-, 50-, 75-, and 100-year analysis periods. For Montana and part of Wyoming, the 30-, 50-, 75, and 100-year analyses included 157, 70, 48, and 12 streamgages, respectively. For those streamgages, nonstationarities were analyzed in the following variables: (1) climatic variables, including annual and seasonal (winter, spring, summer, and fall) temperature and precipitation; (2) daily streamflow variables, including the annual center of volume duration, annual center of volume median, and peaks over threshold with a mean of four events per year; and (3) annual peak-flow variables, including peak-flow timing and magnitude. A likelihood approach was used to express statistical confidence and assign the nonstationarity results as likely upward or downward (highest statistical confidence), somewhat likely upward or downward (less statistical confidence), or about as likely as not (little statistical confidence; hereinafter, neutral). For the nonstationarity analyses of the climatic, daily streamflow, and peak-flow variables, the results are presented in detail and discussed with respect to statewide patterns and geographic variability. For each of the 30-, 50-, and 75-year analyses, peak-flow change-point and monotonic trend analyses were compiled for streamgages classified with likely downward or likely upward trends. For those streamgages, the associated basin characteristics and nonstationarity results for peak-flow timing, daily streamflow, and climatic variables were investigated and statistically compared to discern associations among other variables that might contribute to the peak-flow nonstationarity results. The 50- and 75-year peak-flow nonstationarities identified in this study are mostly downward, in association with mostly upward temperature and potential evapotranspiration:precipitation monotonic trends. For the 50-, 75-, and 100-year analyses, the peak-flow change points are predominantly downward and are concentrated in the 1970s and 1980s, which indicates general consistency among the longer trend periods. These findings are in association with substantial research documenting globally rising temperature and atmospheric greenhouse gas concentrations that might be largely attributed to anthropogenic activities. Anthropogenic effects might represent long-term (on the order of several decades to more than a century) climate changes that might happen within highly variable natural climate fluctuations. Several paleo studies in the north-central United States have indicated that hydroclimatic extremes (that is, low- and high-streamflow conditions) before European settlement have been outside of extremes since the 1900s. Depending on the interactions of anthropogenic effects and natural climate variability, extreme high-streamflow conditions might occur in the future, even in the presence of long-term downward peak-flow trends.

Montana, Wyoming↗

USGS2024

Dynamic treeline and cryosphere response to pronounced mid-Holocene climatic variability in the US Rocky Mountains

Climate-driven changes in high-elevation forest distribution and reductions in snow and ice cover have major implications for ecosystems and global water security. In the Greater Yellowstone Ecosystem of the Rocky Mountains (United States), recent melting of a high-elevation (3,091 m asl) ice patch exposed a mature stand of whitebark pine ( Pinus albicaulis ) trees, located ~180 m in elevation above modern treeline, that date to the mid-Holocene (c. 5,950 to 5,440 cal y BP). Here, we used this subfossil wood record to develop tree-ring-based temperature estimates for the upper-elevation climate conditions that resulted in ancient forest establishment and growth and the subsequent regional ice-patch growth and downslope shift of treeline. Results suggest that mid-Holocene forest establishment and growth occurred under warm-season (May-Oct) mean temperatures of 6.2 °C (±0.2 °C), until a multicentury cooling anomaly suppressed temperatures below 5.8 °C, resulting in stand mortality by c. 5,440 y BP. Transient climate model simulations indicate that regional cooling was driven by changes in summer insolation and Northern Hemisphere volcanism. The initial cooling event was followed centuries later (c. 5,100 y BP) by sustained Icelandic volcanic eruptions that forced a centennial-scale 1.0 °C summer cooling anomaly and led to rapid ice-patch growth and preservation of the trees. With recent warming (c. 2000–2020 CE), warm-season temperatures now equal and will soon exceed those of the mid-Holocene period of high treeline. It is likely that perennial ice cover will again disappear from the region, and treeline may expand upslope so long as plant-available moisture and disturbance are not limiting.

Montana, Wyoming↗

USGS2024

Functional turnover in a prairie-river fish community over 130 years

Objective In many Great Plains rivers, functional turnover—the change in proportional dominance of members in biological communities that fill certain ecological roles—has occurred due to impoundment and habitat alteration. The Powder River of Montana and Wyoming remains one of the few unregulated prairie rivers, but long-term monitoring is limited, so we analyzed changes over time at the functional, assemblage, and species levels. Methods We used fish sampling data from 43 different sources collected from 1893 to 2022 to analyze trends in fish communities. Result Across the main-stem Powder River, Sand Shiner Miniellus stramineus and Channel Catfish Ictalurus punctatus substantially increased in abundance, whereas Sturgeon Chub Macrhybopsis gelida decreased. While most other species did not show significant changes in relative abundance (although the always rare Lake Chub Couesius plumbeus may have been extirpated), significant functional turnover occurred in the upper river due to increases in generalist feeders, predators, omnivores, and cavity-guarding species, with declines in benthic feeders, invertivores, and pelagic broadcast spawners, among others. Community and functional changes were more substantial in the upper river than in the lower river, possibly due to augmented streamflow from a major tributary. Conclusion Functional turnover within the upper river was substantial despite the relative stability of most individual species, even when the Sand Shiner—the most significantly increasing species—was excluded from analysis. This suggests small but consistent increases and decreases within functional groups, which cumulatively are likely impacting the ecosystem. We hypothesize a complex set of mechanisms causing these changes that offer avenues for future work. The collation of data from disparate studies and the resampling of even a limited number of historical fish collection locations can greatly aid in identifying potential fish community changes in systems where monitoring is limited.

Montana, Wyoming↗

USGS2024

Declining geomorphic diversity and potential adaptive management opportunities on a highly regulated reach of the Bighorn River, Montana

Rivers downstream from dams often experience decreased flow variability and disrupted sediment transport. We investigated a highly regulated 35.5-kilometre reach of the Bighorn River, downstream from Yellowtail Dam, emplaced in 1965. This dam created a thriving trout fishery, but more recently, side channel networks and habitat diminished. We document how the Bighorn River's anabranching morphology responded to flow regulation and a near cessation of sediment supply. Geomorphic diversity drastically decreased since 1939 and the Bighorn River abandoned numerous side channels. By 1980, geomorphic loss slowed, and the river became laterally static. The 1980 river and side channel network could represent the maximum areal extent of a downscaled morphology, maintained through restoration and adaptive management. However, side channel restoration alone will not return natural alluvial processes to this river reach. The Bighorn River needs sediment to reinstate dynamic lateral movement, even within a downscaled morphology. Sediment augmentation is an option, but more complex to implement on rivers where potential downstream impacts and landowners must be considered. The decline of geomorphic diversity and potential adaptive management solutions on the Bighorn River has wide applicability to numerous dammed rivers, where similar changes to the flow and sediment transport regime are common.

Montana, Wyoming↗

USGS2023

Organic layers preserved in ice patches: A new record of Holocene environmental change on the Beartooth Plateau, USA

Growing season temperatures play a crucial role in controlling treeline elevation at regional to global scales. However, understanding of treeline dynamics in response to long-term changes in temperature is limited. In this study, we analyze pollen, plant macrofossils, and charcoal preserved in organic layers within a 10,400-year-old ice patch and in sediment from a 6000-year-old wetland located above present-day treeline in the Beartooth Mountains, Wyoming, to explore the relationship between Holocene climate variability and shifts in treeline elevation. Pollen data indicate a lower-than-present treeline between 9000 and 6200 cal yr BP during the warm, dry summer and cold winter conditions of the early Holocene. Increases in arboreal pollen at 6200 cal yr BP suggest an upslope treeline expansion when summers became cooler and wetter. A possible hiatus in the wetland record at ca. 4200–3000 cal yr BP suggests increased snow and ice cover at high elevations and a lowering of treeline. Treeline position continued to fluctuate with growing season warming and cooling during the late-Holocene. Periods of high fire activity correspond with times of increased woody cover at high elevations. The two records indicate that climate was an important driver of vegetation and treeline change during the Holocene. Early Holocene treeline was governed by moisture limitations, whereas late-Holocene treeline was sensitive to increases in growing season temperatures. Climate projections for the region suggest warmer temperatures could decrease effective growing season moisture at high elevations resulting in a reduction of treeline elevation.

Montana, Wyoming↗

USGS2023

Spatial variation in density of American black bears in northern Yellowstone National Park

The quality and availability of resources are known to influence spatial patterns of animal density. In Yellowstone National Park, relationships between the availability of resources and the distribution of grizzly bears ( Ursus arctos ) have been explored but have yet to be examined in American black bears ( Ursus americanus ). We conducted non-invasive genetic sampling during 2017–2018 (mid-May to mid-July) and applied spatially explicit capture-recapture models to estimate density of black bears and examine associations with landscape features. In both years, density estimates were higher in forested vegetation communities, which provide food resources and thermal and security cover preferred by black bears, compared with non-forested areas. In 2017, density also varied by sex, with female densities being higher than males. Based on our estimates, the northern range of Yellowstone National Park supports one of the highest densities of black bears (20 black bears/100 km 2 ) in the northern Rocky Mountains (6–12 black bears/100 km 2 in other regions). Given these high densities, black bears could influence other wildlife populations more than previously thought, such as through displacement of sympatric predators from kills. Our study provides the first spatially explicit estimates of density for black bears within an ecosystem that contains the majority of North America's large mammal species. Our density estimates provide a baseline that can be used for future research and management decisions of black bears, including efforts to reduce human–bear conflicts.

Montana, Wyoming↗

USGS2023

Multi-level thresholds of residential and agricultural land use for elk avoidance across the Greater Yellowstone Ecosystem

1. Conversion of land for settlements and agriculture is increasing globally and can influence wildlife space use. However, there is limited research to identify the thresholds of land-use change that incur wildlife avoidance and how these thresh-olds might vary across levels of selection. 2. We evaluated multi-level avoidance thresholds of elk Cervus canadensis impacted by residential development and irrigated agriculture across the Greater Yellowstone Ecosystem in Idaho, Montana and Wyoming. Using GPS data from765 elk in 21 herds, we estimated habitat selection in relation to development and agriculture at three levels (home range selection, within home range selection and movement path selection). Next, using individual selection covariates and as-sociated measures of land-use availability, we used functional-response models to evaluate how selection varied based on availability, and in turn, to estimate avoidance thresholds. 3. We found individual and level-specific variation in elk responses to environmental factors. Elk exhibited stronger responses (either selection or avoidance) when selecting home range locations (i.e. second-order selection) than when selecting areas within home ranges (i.e. third-order selection) or selecting movement paths (i.e. fourth-order selection). Importantly, elk avoidance of development and agriculture changed as the amount of land in these categories changed. Across all levels of selection elk exhibited neutral selection for human development at low levels of availability (<1.1%–2.2% developed) but avoided areas that were >1.1%–2.2% developed. Conversely, elk selected positively for irrigated agriculture at low to moderate levels of availability (<52.0%–66.2% agriculture) but exhibited neutral selection in areas that were >52.0%–66.2% agriculture. 4. Synthesis and applications . Elk avoidance of low levels of human development suggests conservation efforts such as restrictions on future development or conservation easements could focus on areas that are still below 2% developed. Additionally, because elk selection was strongest at the landscape scale, conservation actions that are based on information about the overall landscape structure may be most impactful. Our results highlight the importance of under-standing variability in wildlife habitat selection at multiple levels, particularly in relation to land-use change, and highlight how functional response modelling can help inform landscape conservation.

Montana, Wyoming↗

USGS2022

The importance of phenology and thermal exposure to early life history success of nonnative Smallmouth Bass in the Yellowstone River

Knowledge of potential spread by introduced species is critical to effective management and conservation. The Smallmouth Bass Micropterus dolomieu is an example of a fish that has been introduced globally, often spreads after introduction, and has substantial predatory impacts on fish assemblages. Nonnative Smallmouth Bass in the free-flowing Yellowstone River, Montana, have expanded from warmer, downstream sections of river into colder, upstream sections containing socio-economically valuable trout fisheries. We sought insight into mechanisms controlling upstream spread by evaluating whether progressively colder upstream climates physiologically constrained successful recruitment by limiting age-0 growth and preventing overwinter survival (i.e., population establishment). We documented the phenology, growth, and overwinter survival of age-0 Smallmouth Bass across a temperature gradient leading to their upstream extent in the Yellowstone River. The upstream extent of population establishment did not appear limited by water temperature alone. Age-0 body size at the onset of winter did not differ significantly between colder, upstream reaches and warmer, downstream reaches. Instead, the earlier hatch timing exhibited by some age-0 individuals in upstream sections allowed them to experience longer growing seasons than many individuals in downstream sections. This counter-intuitive hatching phenology mediated much of the expected decreases in growth in colder, upstream climates. Furthermore, evidence of successful overwinter survival and simulations of age-0 starvation mortality indicated that age-0 individuals at the upstream extent of their distribution successfully recruited to the age-1 year-class during four consecutive years. However, age-0 individuals were rare or absent throughout the uppermost upstream distribution of adults, suggesting that something other than temperature limits or discourages reproduction farther upstream. Taken together, our results suggest that Smallmouth Bass have not yet reached the thermal limit of their upstream distribution in the Yellowstone River and that future spread may challenge fisheries managers tasked with management of coldwater trout fisheries in this river.

Montana, Wyoming↗

USGS2022

Average kinship within bighorn sheep populations is associated with connectivity, augmentation, and bottlenecks

Understanding the influence of population attributes on genetic diversity is important to advancement of biological conservation. Because bighorn sheep ( Ovis canadensis ) populations vary in size and management history, the species provides a unique opportunity to observe the response of average pairwise kinship, inversely related to genetic diversity, to a spectrum of natural and management influences. We estimated average pairwise kinship of bighorn sheep herds and compared estimates with population origin (native/indigenous/extant or reintroduced), historical minimum count, connectivity, and augmentation history, to determine which predictors were the most important. We evaluated 488 bighorn sheep from 19 wild populations with past minimum counts of 16–562 animals, including native and reintroduced populations that received 0–165 animals in augmentations. Using the Illumina High Density Ovine array, we generated a dataset of 7728 single nucleotide polymorphisms and calculated average pairwise kinship for each population. Multiple linear regression analysis determined that connectivity between populations via dispersal, greater number of animals received in augmentations, and greater minimum count were correlated with lower average pairwise kinship at the population level, and whether the population was extant or reintroduced was less important. Thus, our results indicated that genetic isolation of populations can result in increased levels of inbreeding. By determining that natural and human-assisted gene flow were likely the most important influences of average pairwise kinship at the population level, this study can serve as a benchmark for future management of bighorn sheep populations and aid in identifying populations of genetic concern to define priorities for conservation of wild populations.

Montana, Wyoming↗

USGS2022

Modelling physiological costs to assess impacts of climate change on amphibians in Yellowstone National Park, U.S.A

Amphibians are vital elements of ecosystems, serving as predator and prey. Their biphasic nature makes them dependent on aquatic and terrestrial habitats; as wet-skinned ectotherms, they are vulnerable to a range of environmental threats, including climate change. Yellowstone National Park (YNP) is becoming warmer and drier, and some wetlands important to amphibians have diminished. Continued climate change is predicted to reduce snowpack, soil moisture, and forest cover. We used data from models of future climate and vegetation cover to mechanistically model how climate change might affect the movements of Western Toads ( Anaxyrus boreas ) across the landscape of three test areas in YNP for the years 2050 and 2090, compared to 2000 as a baseline. Least-cost path analysis produced mixed results: for 2050 and 2090, physiological costs of movement increased in one test area and decreased in another; they were mixed in the third. These changes generally reflect the preference by toads for more open forests. Estimating costs for other species of YNP amphibians produced more negative results. For Columbia Spotted Frogs ( Rana luteiventris ) and Boreal Chorus Frogs ( Pseudacris maculata ) (both more aquatic and less adapted to terrestrial habitats), movement costs increased by about 2–15X. Reduced frequency or duration of rain events might limit the nocturnal movements of Western Tiger Salamanders ( Ambystoma mavortium ). Climate change may not have negative impacts on all amphibians throughout YNP, but increased movement costs for terrestrial habitats will accentuate effects of drying wetlands in at least parts of YNP. Land management actions that preserve habitat structure of both forest and low shrub cover may help mitigate continued drying conditions of climate change.

Montana, Wyoming↗

USGS2021

Responses of American black bears to spring resources

In temperate regions of the world, food resources are seasonally limited, which causes some wildlife species to seek out nutrient-rich resources to better meet their caloric needs. Animals that utilize high-quality resources may reap fitness benefits as they prepare for mating, migration, or hibernation. American black bears ( Ursus americanus ) are omnivores that consume both plant and animal food resources to meet macronutrient needs. Black bears capitalize on high-quality food resources, such as soft mast in summer and hard mast during autumn, but we know less about the importance of resource quality during spring. Therefore, we sought to understand the relationship between the spatiotemporal variation in the availability of food and resource selection of black bears during spring. We also aimed to infer potential changes in foraging tactics, from opportunistic foraging to more active selection. Although black bears are described as opportunistic omnivores, we hypothesized they select areas with high-quality forage when available. We instrumented 7 black bears with GPS collars in 2017 and 2018 and estimated fine-scale resource selection with integrated step-selection functions. We found evidence that black bear movements were influenced by forage quality of vegetative food resources. However, we failed to find evidence that black bears actively alter their movements to take advantage of seasonal neonate elk. Although black bears represent a substantial cause of mortality for neonate elk, we found that black bears likely feed on neonates encountered opportunistically while traveling between patches of high-quality forage. Few studies have shown evidence of an omnivorous species capitalizing on spatiotemporal variation in forage quality, yet our data suggest this may be an important strategy for species with diverse diets, particularly where resources are seasonally limited.

Montana, Wyoming↗

USGS2021

Activity-based, genome-resolved metagenomics uncovers key populations and pathways involved in subsurface conversions of coal to methane

Microbial metabolisms and interactions that facilitate subsurface conversions of recalcitrant carbon to methane are poorly understood. We deployed an in situ enrichment device in a subsurface coal seam in the Powder River Basin (PRB), USA, and used BONCAT-FACS-Metagenomics to identify translationally active populations involved in methane generation from a variety of coal-derived aromatic hydrocarbons. From the active fraction, high-quality metagenome-assembled genomes (MAGs) were recovered for the acetoclastic methanogen, Methanothrix paradoxum , and a novel member of the Chlorobi with the potential to generate acetate via the Pta-Ack pathway. Members of the Bacteroides and Geobacter also encoded Pta-Ack and together, all four populations had the putative ability to degrade ethylbenzene, phenylphosphate, phenylethanol, toluene, xylene, and phenol. Metabolic reconstructions, gene analyses, and environmental parameters also indicated that redox fluctuations likely promote facultative energy metabolisms in the coal seam. The active “ Chlorobi PRB” MAG encoded enzymes for fermentation, nitrate reduction, and multiple oxygenases with varying binding affinities for oxygen. “ M. paradoxum PRB” encoded an extradiol dioxygenase for aerobic phenylacetate degradation, which was also present in previously published Methanothrix genomes. These observations outline underlying processes for bio-methane from subbituminous coal by translationally active populations and demonstrate activity-based metagenomics as a powerful strategy in next generation physiology to understand ecologically relevant microbial populations.

Montana, Wyoming↗

USGS2021

Comparative effects of energy-related saline wastewaters and sodium chloride on hatching, survival, and fitness-associated traits of two amphibian species

Salinity (sodium chloride [NaCl]) is a prevalent and persistent contaminant that negatively affects freshwater ecosystems. Although most studies focus on effects of salinity from road salts (primarily NaCl), high-salinity wastewaters from energy extraction (wastewaters) could be more harmful because they contain NaCl and other toxic components. Many amphibians are sensitive to salinity, and their eggs are thought to be the most sensitive life-history stage. However, there are few investigations with salinity that include eggs and larvae sequentially in long-term exposures. We investigated the relative effects of wastewaters from a large energy reserve, the Williston Basin (USA), and NaCl on northern leopard ( Rana pipiens ) and boreal chorus ( Pseudacris maculata ) frogs. We exposed eggs and tracked responses through larval stages (for 24 days). Wastewaters and NaCl caused similar reductions in hatching and larval survival, growth, development, and activity, while also increasing deformities. Chorus frog eggs and larvae were more sensitive to salinity than leopard frogs, suggesting species-specific responses. Contrary to previous studies, eggs of both species were less sensitive to salinity than larvae. Our ecologically relevant exposures suggest that accumulating effects can reduce survival relative to starting experiments with unexposed larvae. Alternatively, egg casings of some species may provide some protection against salinity. Notably, effects of wastewaters on amphibians were predominantly due to NaCl rather than other components. Therefore, findings from studies with other sources of increased salinity (e.g., road salts) could guide management of wastewater-contaminated ecosystems, and vice versa, to mitigate effects of salinization.

Montana, Wyoming↗

USGS2021

Effect of an algal amendment on the microbial conversion of coal to methane at different sulfate concentrations from the Powder River Basin, USA

Biogenic methane is estimated to account for one-fifth of the natural gas worldwide and there is great interest in controlling methane from different sources. Biogenic coalbed methane (CBM) production relies on syntrophic associations between fermentative bacteria and methanogenic archaea to anaerobically degrade recalcitrant coal and produce methanogenic substrates. However, very little is known about how differences in geochemistry, hydrology, and microbial community composition influence subsurface carbon utilization and CBM production. The addition of an amendment consisting of microalgal biomass has previously been shown to increase CBM production while providing the possibility of a closed-loop fossil system where waste (production water) is used to grow algae to ultimately produce energy (methane). However, the efficiency of enhancing CBM production under different redox conditions remains unresolved. In this study, we focused on the U.S. Geological Survey's Birney test site (Montana, USA) that has nine wells vertically accessing four coal seams with varying geochemistry (low and high sulfate (SO 4 2− )) and methane production rates. We used organic matter (OM) in the form of algal biomass to discern the effect of this amendment on OM degradation and microbially enhanced CBM production potential under different geochemical constraints. We tracked changes in community composition, OM composition, organic carbon (OC) concentration, methane production, and nutrients in batch systems over six months. Methane production was detected only in microcosms from low SO 4 2− wells (168 to 800 μg methane per gram of coal). The OC consumption varied across time for all wells and the variation was greatest for the low SO 4 2− wells. Different groups of syntrophic bacteria were associated with net‑carbon consuming microcosms, and specifically Syntrophorhabdus was identified with several different statistical methods as a potentially important coal degrader. Results from this study provide insight into potential coal-degraders, the compositional changes in some of the different OM fractions, and trends in carbon consumption related to methane production across coal seams along the vertical SO 4 2− gradient.

Montana, Wyoming↗

USGS2021

Cold tolerance of mountain stoneflies (Plecoptera: Nemouridae) from the high Rocky Mountains

How aquatic insects cope with cold temperatures is poorly understood. This is particularly true for high-elevation species, which often experience a seasonal risk of freezing. In the Rocky Mountains, nemourid stoneflies (Plecoptera: Nemouridae) are a major component of mountain stream biodiversity and are typically found in streams fed by glaciers and snowfields, which are rapidly receding due to climate change. Predicting the effects of climate change on mountain stoneflies is difficult because their thermal physiology is largely unknown. We investigated cold tolerance of several alpine stoneflies ( Lednia tumana , Lednia tetonica , and Zapada spp.) from the Rocky Mountains, USA. We measured the supercooling point (SCP) and tolerance to ice enclosure of late-instar nymphs collected from a range of thermal regimes. SCPs varied among species and populations, with the lowest SCP measured for nymphs from an alpine pond, which was much more likely to freeze solid in winter than flowing streams. We also show that L. tumana cannot survive being enclosed in ice, even for short periods of time (<3 h) at relatively mild temperatures (–0.5 °C). Our results indicate that high-elevation stoneflies at greater risk of freezing may have correspondingly lower SCPs, and despite their common association with glacial meltwater, these stoneflies appear to be living near their lower thermal limits.

Montana, Wyoming↗