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Research about Alabama

Source-linked reports with geographic coverage including Alabama.

At least 37 records · Page 2Linked to original sources

Mapping karst groundwater flow paths and delineating recharge areas for Fern Cave, Alabama, through the use of dye tracing

Fern Cave in Jackson County, Alabama, is a 15.6-mile-long (25.1-kilometer) cave system, managed by the U.S. Fish and Wildlife Service and Southeastern Cave Conservancy, that has the second highest biodiversity of any cave in the southeastern United States. Groundwater in karst ecosystems is known to be susceptible to impacts from human-induced land-use activities in watersheds that contribute recharge to the groundwater system. To provide the U.S. Fish and Wildlife Service with necessary baseline information on the groundwater flow system in Fern Cave, the U.S. Geological Survey and the Kentucky Geological Survey conducted a series of dye traces during 2019–21 to delineate the watershed recharging the cave system. The dye traces identified two separate streams that flow through the cave and a recharge area of 1.73 square miles (4.48 square kilometers) draining to the cave system. Current land use within the recharge area is dominated by deciduous forest with minimal additional land use types, indicating a low potential for undesirable effects to the cave by anthropogenic sources.

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Modeling the effects of interior headland restoration on estuarine sediment transport processes in a marine-dominant estuary

The effects of interior headland restoration on estuarine sediment transport processes were assessed through process-based numerical modeling. Three proposed interior headland restoration scenarios in the Grand Bay estuary (Mississippi/Alabama) were modeled using Delft3D to understand impacts on suspended sediment concentrations, bed level morphology, and sediment fluxes under present-day conditions and a sea level rise (SLR) of 0.5 m, representing a high projection of SLR by the year 2050. Model results showed localized differences in bed levels near the restored features after a year of simulated morphologic change. The restored headland features acted as a sediment source to the immediate surroundings while also providing some non-significant sheltering effect of backshore shoals and marsh shorelines. Sediment fluxes were sensitive to wind directions and the presence of the restored headlands. However, regardless of wind direction, mean sea level, or restoration action, the greatest sediment fluxes were always export fluxes from the estuary, which were further increased with increased sea level. Suspended sediment concentrations were highly influenced by SLR in a non-linear manner. Sediment concentrations both increased and decreased depending on depth under SLR. Furthermore, SLR allowed for the suspension and deposition of sediments on the marsh platform. Overall, the influence of SLR was more impactful to changing sediment dynamics than the influence of the restoration features.

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Modeling the effects of large-scale interior headland restoration on tidal hydrodynamics and salinity transport in an open coast, marine-dominant estuary

The effects of large-scale interior headland restoration on tidal hydrodynamics and salinity transport in an open coast, marine dominant estuary (Grand Bay, Alabama, U.S.A) are investigated using a two-dimensional model, the Discontinuous-Galerkin Shallow Water Equations Model (DG-SWEM). Three restoration alternatives are simulated for present-day conditions, as well as under 0.5 m of sea level rise (SLR). Model results show that the restoration alternatives have no impact on tidal range within the estuary but change maximum tidal velocities by ±5 cm/s in the present-day scenarios and by ±7 cm/s in the scenarios with 0.5 m of SLR. Differences in average salinity concentrations for simulated tropical and frontal seasons show increases and decreases on the order of 2 pss in the embayments surrounding the restoration alternatives; differences were larger (on the order of ±4 pss) for the scenarios with 0.5 m of SLR. There were minimal changes in average salinity outside of the estuary and no changes offshore. The size and position of the alternatives played a role in the salinity response as a result of changing the estuarine shoreline geometry and affecting the fetch within the bay. SLR was more impactful in increasing exposure to low salinity values (i.e., less than 5 pss) than the presence of the restoration alternatives. Overall, the modeled results indicate that these large-scale restoration actions have limited and localized impacts on the hydrodynamics and salinity patterns in this open coast estuary. The results also demonstrate the nonlinear response of salinity to SLR, with increases and decreases in the maximum, mean and minimum daily salinity concentrations from present-day conditions. This nonlinear response was a result of changes in the directions of the residual currents, which affected salinity transport.

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Cryptic tolerant fish species and their potential effect on index of biotic integrity (IBI) scores

Indices such as the Index of Biotic Integrity (IBI) are often used by management agencies to estimate the abstract property of stream health. These indices are usually predicated on the belief that certain fish species are tolerant to environmental perturbation while others are sensitive. Species are usually designated as either tolerant or sensitive in these analyses based on inherent ecological or taxonomic characteristics. However, previous literature has shown that certain species from ecological or taxonomic “sensitive” groups experience increased abundance in degraded streams. We term such species “cryptic tolerants”. Using a stream fish assemblage dataset of 433 unique sample locations across the state of Alabama and the National Landcover Dataset, our objectives were to 1) identify the most common cryptic tolerant species, 2) investigate how cryptic tolerant species might inflate indices of stream health, and 3) compare an alternative measure of stream health in which species are statistically defined rather than defined using the traditional trait-based approach. We identified cryptic tolerants using Nonmetric Multidimensional Scaling in six ecoregions. A series of regressions revealed that the proportion of cryptic tolerant species decreased in response to an increasing proportion of forested land in catchments while the proportion of true sensitives increased in all ecoregions except for the Cumberland Plateau. An index that simply used the percentage of statistically defined, non-tolerant species generally had lower p-values and higher r 2 values than IBI scores when both were regressed against percentage of forest in catchment. However, both indices had low degrees of correlation with expected disturbance, indicating a univariate index may be inadequate to characterize stream health. Our results highlight a potential issue with applying the IBI to diverse southeastern systems in the United States, which may be alleviated by designating species sensitivity based on empirical response to disturbance rather than taxonomic or ecological characteristics.

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Long short-term memory models to quantify long-term evolution of streamflow discharge and groundwater depth in Alabama

Long short-term memory (LSTM) models have been shown to be efficient for rainfall-runoff modeling, and to a lesser extent, for groundwater depth forecasting. In this study, LSTMs were applied to quantify the spatiotemporal evolution of surface and subsurface hydrographs in Alabama in the Southeastern United States, where water sustainability has not been fully quantified across spatiotemporal scales. First, the surface water LSTM model with extensive dynamic (precipitation and other weather variables) and static (basin characteristics) inputs predicted the main characteristics of streamflow for six years at 19 gauged basins in Alabama. The model tended to underestimate extremely high streamflow but adding drainage density as an input feature slightly improved the predictions of extreme events. Second, to predict the groundwater depth evolution, a groundwater LSTM (GW-LSTM) model was proposed and applied using static inputs capturing the aquifers' hydrogeological properties and dynamic inputs of meteorological information. Three precipitation scenarios were also explored to evaluate the groundwater hydrograph evolution in the next two decades. The GW-LSTM model predicted the general trend of daily groundwater depth fluctuations (at 21 wells distributed across Alabama from 1990 to 2021) including most extremely high groundwater levels, and recovered groundwater depth for locations withheld from model training and validation. This study, therefore, extended the application of LSTMs in quantifying the spatiotemporal evolution of surface water and groundwater, two manifestations of a single integrated resource.

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Decision science as a framework for combining geomorphological and ecological modeling for the management of coastal systems

The loss of ecosystem services due to climate change and coastal development is projected to have significant impacts on local economies and conservation of natural resources. Consequently, there has been an increase in coastal management activities such as living shorelines, oyster reef restoration, marsh restoration, beach and dune nourishment, and revegetation projects. Coastal management decisions are complex and include challenging trade-offs. Decision science offers a useful framework to address such complex problems. Here, we provide a synthesis about how decision science can help to integrate research from multiple disciplines (physical and life sciences) with management of coastal and marine systems. Specifically, we discuss the importance of considering concepts and techniques from ecology, coastal geology, geomorphology, climate science, oceanography, and decision analysis when developing conservation plans for coastal restoration. We illustrate the process with several coastal restoration studies. Our capstone example is based on a recent barrier island restoration assessment project at Dauphin Island, Alabama, which included the development of geomorphological and ecological models. We show how decision science can be used as a framework to combine geomorphological and ecological modeling to help inform management decisions while considering uncertainty about system changes and risk tolerance. We also build on our examples through a review of recently developed techniques for spatial conservation planning for land acquisition decisions and the application of adaptive management for sequential decisions.

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Adult white-tailed deer survival in hunted populations on public and private lands

Estimates of sex- and age-specific survival are important for guiding population management decisions for white-tailed deer ( Odocoileus virginianus ). However, differences in deer survival between public and private lands can exist and, if unaccounted for, may affect wildlife agencies' ability to effectively manage statewide and local deer populations. From 2014 to 2016, we radiocollared and monitored survival of 79 adult white-tailed deer (141 deer-years; 62 male, 79 female) on 2 public and 2 private properties in Alabama, USA. We assessed the effects of sex, age, and landownership type (i.e., public or private) on adult white-tailed deer harvest and survival patterns using an information-theoretic approach. Hunter harvest accounted for 77% of observed mortalities ( n = 23/30), but harvest and survival rates varied by sex and age. Harvest and survival were similar between public and private property despite more restrictive hunting regulations for antlered deer on public areas. Similar harvest rates were likely due to self-imposed, quality deer management (QDM) harvest strategies on private land that emulated the effects of a state-mandated antler point restriction (APR) on public land. Our findings indicate survival rates of adult white-tailed deer may be applicable across landownership types where state-mandated harvest regulations with respect to males are more restrictive on public than private property, due to the popularity of QDM by private-land deer hunters. However, where regulations are similar across landownership types (e.g., no state-mandated APR), differences in harvest and survival rates may exist.

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Alabama and Landsat

Alabama’s warm climate and rich soil bolster its agriculture and timber industries, but they also offer ideal conditions for natural playgrounds, enjoyed by humans and the wildlife that call the open green spaces home. Alabama has 21 State parks and 11 national parks, monuments, and trails across its diverse geography. Cotton is no longer king in the Cotton State, but it remains a part of the fabric of its multibillion-dollar agriculture sector. The State also produces poultry, cattle, calves, corn, lumber, soybeans, and catfish. The State is home to Dauphin Island, the first land mass seen by migratory birds and pollinators upon their return from South America. Dauphin Island acts as a landing zone for hundreds of species, including sandpipers, plovers, and herons, and protects Alabama’s coastline. Since 1961, Dauphin Island has been the home of the Audubon Bird Sanctuary. The varied landscapes of the Cotton State face challenges from climate change, land change, and extreme weather. Satellite imagery from the U.S. Geological Survey Landsat Program can offer insight and understanding in these and other areas. Here are some ways Landsat benefits Alabama.

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The potential of wave energy conversion to mitigate coastal erosion from hurricanes

Wave energy conversion technologies have recently attracted more attention as part of global efforts to replace fossil fuels with renewable energy resources. While ocean waves can provide renewable energy, they can also be destructive to coastal areas that are often densely populated and vulnerable to coastal erosion. There have been a variety of efforts to mitigate the impacts of wave- and storm-induced erosion; however, they are either temporary solutions or approaches that are not able to adapt to a changing climate. This study explores a green and sustainable approach to mitigating coastal erosion from hurricanes through wave energy conversion. A barrier island, Dauphin Island, off the coast of Alabama, is used as a test case. The potential use of wave energy converter farms to mitigate erosion due to hurricane storm surges while simultaneously generating renewable energy is explored through simulations that are forced with storm data using the XBeach model. It is shown that wave farms can impact coastal morphodynamics and have the potential to reduce dune and beach erosion, predominantly in the western portion of the island. The capacity of wave farms to influence coastal morphodynamics varies with the storm intensity.

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The use of dye-tracing studies to delineate the recharge area for Manitou Cave in northwestern Alabama

In 2010 the U.S. Fish and Wildlife (USFWS) was petitioned to federally list the Manitou Cave Snail, (Antrorbis breweri ), a stygobiont endemic to Manitou Cave in northwestern Alabama. When an agency is tasked with determining whether to add a species to the Endangered Species List, one of the components examined is potential threats to the biota. Knowing the recharge area for a cave or spring is critical to identifying potential threats because of the interconnectivity between surface activities and groundwater quality/quantity in karst areas. Potential threats to water quality and quantity in Manitou Cave include recent subdivision developments, a nearby active quarry, land clearing, and several major highways. The 1.7 km-long cave is currently owned by a non-profit organization, Manitou Cave of Alabama, which oversees management, restoration, research, and documentation activities. In 2016, talks began between state and federal agencies and the new management in an effort to initiate new research to determine whether the snail merited listing. In 2019, a cooperative project between USFWS and the U.S. Geological Survey was started to delineate a recharge area for Manitou Cave through dye tracing. The current research will be used by USFWS to determine primary threats and to inform the decision of whether and how to list the Manitou Cave Snail under the Endangered Species Act.

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Effects of culvert construction on streams and macroinvertebrate communities at selected sites in the East Gulf Coastal Plain of Alabama, 2010–19

The U.S. Geological Survey, in cooperation with the Alabama Department of Transportation, evaluated the role of culvert construction in altering streams and habitats of benthic macroinvertebrate communities at selected study sites in the northern East Gulf Coastal Plain of Alabama during 2011–19. Analysis included examinations of changes in stream channel geometry, suspended sediment, turbidity, and benthic macroinvertebrate communities. Topographic surveys of stream channel cross sections, upstream and downstream of the culvert, were conducted before and after construction. Changes in channel geometry (cross-sectional area, top width, mean depth, and thalweg slope) were assessed by using paired sample t-tests to compare before- and after-construction channel geometry measurements. Statistically significant changes in stream channel geometry between the before- and after-construction measurements were observed at four of the six study sites. Analysis of the channel geometry data indicates that 1 site had no measured changes, and thalweg reach slopes were inverted at 4 of the 12 study reaches—2 measured in before-construction reaches and 2 measured in after-construction reaches. Surface-water samples were collected during selected storm events for suspended sediment and turbidity analyses. Samples were simultaneously collected upstream and downstream of the culvert construction reaches during all three phases of construction (before, during, and after). Analysis focused on the parity of upstream to downstream simultaneous samples. The mean upstream to downstream paired ratios of sediment concentrations and turbidity from the after-construction phase indicate that colloidal and noncolloidal sediments were passing through the construction reaches at two of the six sites, noncolloidal sediments were being trapped in the construction reaches at two sites, and colloidal and noncolloidal sediments were being removed from the construction reach at two sites. Benthic macroinvertebrates were collected and identified at five of the six sites from instream habitats that were available in sampled areas both upstream and downstream of the culvert construction reaches. Differences between upstream and downstream reaches and the Wilcoxon rank sum statistic were used to examine changes in metrics of benthic macroinvertebrate communities between before- and after-construction phases. Benthic macroinvertebrate sampling results did not indicate that culvert construction caused impairment to communities at study sites. No tolerance metrics suggested a major change in the pollution tolerance of the communities. The same upstream to downstream patterns in abundance-weighted tolerance values were observed in the before- and after-construction periods at each site. At one site, the difference between upstream and downstream richness-based tolerance values increased, but the after-construction upstream and downstream richness-based tolerance values were lower (indicating a less pollution-tolerant macroinvertebrate community) than in the before-construction period.

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Assessing habitat change and migration of barrier islands

Barrier islands are dynamic environments that experience gradual change from waves, tides, and currents, and rapid change from extreme storms. These islands are expected to change drastically over the coming century due to accelerated sea-level rise and changes in frequency and intensity of storm events. The dynamic nature of barrier islands coupled with the importance of these environments make it critical for natural resource managers to understand how habitats on barrier islands are changing or may change over time to determine when and where management actions may be needed. In this study, we applied a habitat change assessment framework, which included exploring areal coverage and distribution changes and change component analysis. Change component analysis, which breaks differences into net gain/loss and allocation difference (i.e., habitat oscillation), has not previously been used to study barrier island habitat evolution. Here, we demonstrate the approach using habitat predictions from a geomorphic modeling effort on Dauphin Island, Alabama (USA). We explored differences of habitat predictions for potential island configurations with and without a beach and dune restoration action under future conditions related to sea level and storminess. We found a potential linkage between landward migration of barrier islands and exchange, an output of change component analysis. The hypothesis may be tested to explore whether this linkage applies over space and time and whether the approach is applicable to monitoring landward migration of coastal wetlands. Collectively, our results highlight the utility of change component analysis for monitoring and quantifying barrier island habitat change and migration.

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Hydrologic effects on growth and hatching success of age-0 Channel Catfish in the Tallapoosa River basin: Implications for management in regulated systems

We assessed the effects of hydrology on growth and hatching success of age‐0 Channel Catfish Ictalurus punctatus in regulated and unregulated reaches of the Tallapoosa River basin, Alabama. Age‐0 Channel Catfish ( N = 91) were collected from sites in both the Coastal Plain and Piedmont regions in fall 2003 and fall 2005. Lapillus otoliths were used to estimate the daily ages of age‐0 Channel Catfish, for which hatch dates were back‐calculated. We performed growth analysis to determine growth histories of each fish at 20‐d increments from hatch. Across the 2 years of sampling, Channel Catfish hatches were documented from June 7 to September 15. Ages and growth rates of age‐0 Channel Catfish ranged from 20 to 126 d and 0.60 to 1.5 mm/d, respectively. In general, growth was highest among age‐0 Channel Catfish from unregulated sites in the lower Coastal Plain, lowest among fish from unregulated sites in the Piedmont, and intermediate from regulated sites in the Piedmont. Successful hatching typically occurred during periods when mean discharges were in the upper two quartiles of flows for each site but not during exceptionally high peaks in flow. Physiographic province, the frequency of high pulses, and the number of flow reversals were the most important factors influencing the growth of recently hatched Channel Catfish. Results suggest that a low to moderate frequency of high pulses (25–150 pulses per 20‐d increment) and a moderate number of flow reversals (~100–175 reversals per 20‐d increment) enhances early growth of Channel Catfish in the Tallapoosa River system. Managing flow, when possible, to minimize large releases of water that result in exceptionally high pulses and providing minimal hydropeaking may improve hatching success during the Channel Catfish spawning season.

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Retreat, detour or advance? Understanding the movements of birds confronting the Gulf of Mexico

During migration, birds must locate stopover habitats that provide sufficient resources to rest and refuel while en route to the breeding or non-breeding area. Long-distance migrants invariably encounter inhospitable geographic features, the edges of which are often characterized by habitat limited in food and safety. In response, they often depart in directions inconsistent with reaching their destination, presumably searching for better habitat. We used automated radio telemetry to track 442 individuals of five species to investigate the behavior of migratory birds as they departed edge habitat along the northern Gulf of Mexico coast during autumn from 2008 to 2014. Most migrants (75%) retreated inland or detoured around rather than advanced across the Gulf, but this depended on bird species and fat-based energy stores. Most individuals in lean condition or of smaller bodied species tended to retreat or detour, rather than advance, when departing from the coast. Twenty-one percent of all birds that departed the coast in 2013–2014 were redetected over 45 km inland, providing a unique opportunity to compare stopover duration, departure times and travel speeds between migrants that retreat away from the coast and those that continue to advance toward their destination. Individuals that retreated the coast and were redetected inland spent ~1 day on the coast before retreating inland, where they spent 11 days before resuming migration. Further when those same individuals retreated from the coast, they departed around evening civil twilight, whereas those that advanced from inland habitats departed after evening civil twilight. Travel speeds were slower for individuals retreating inland compared to those advancing towards the coast from inland habitats. The differences between retreating and advancing individuals suggest how an individual's drive to feed or fly influences behavior. Our study illustrates how the sum of individual decisions can shape habitat use, landscape-scale movements and migration strategies.

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Contrasting patterns of demography and population viability among gopher tortoise (Gopherus polyphemus) populations at the species’ northern range edge

Population viability analyses are useful tools to predict abundance and extinction risk for imperiled species. In southeastern North America, the federally threatened gopher tortoise ( Gopherus polyphemus ) is a keystone species in the diverse and imperiled longleaf pine ( Pinus palustris ) ecosystem, and researchers have suggested that tortoise populations are declining and characterized by high extinction risk. We report results from a 30-year demographic study of gopher tortoises in southern Alabama (1991–2020), where 3 populations have been stable and 3 others have declined. To better understand the demographic vital rates associated with stable and declining tortoise populations, we used a multi-state hierarchical mark-recapture model to estimate sex- and stage-specific patterns of demographic vital rates at each population. We then built a predictive population model to project population dynamics and evaluate extinction risk in a population viability context. Population structure did not change significantly in stable populations, but juveniles became less abundant in declining populations over 30 years. Apparent survival varied by age, sex, and site; adults had higher survival than juveniles, but female survival was substantially lower in declining populations than in stable ones. Using simulations, we predicted that stable populations with high female survival would persist over the next 100 years but sites with lower female survival would decline, become male-biased, and be at high risk of extirpation. Stable populations were most sensitive to changes in apparent survival of adult females. Because local populations varied greatly in vital rates, our analysis improves upon previous demographic models for northern populations of gopher tortoises by accounting for population-level variation in demographic patterns and, counter to previous model predictions, suggests that small tortoise populations can persist when habitat is managed effectively. © 2021 The Wildlife Society.

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Single-well production history matching and geostatistical modeling as proxy to multi-well reservoir simulation for evaluating dynamic reservoir properties of coal seams

Reservoir properties of coal seams such as gas and water effective permeabilities and gas content, as well as spatial distributions thereof, affect the success of gas production and CO 2 -enhanced gas recovery (EGR) with simultaneous CO 2 sequestration. These properties change during production and injection operations due to variations in reservoir pressure, matrix shrinkage/swelling, and water saturation and are therefore referred to as dynamic properties. Predicting distribution of such important reservoir properties and how they evolve during production, or injection, at unsampled locations can be particularly important for field development and project economics. In this work, dynamic properties of Black Creek coal seam of Black Warrior Basin, Alabama were mapped using pointwise results from single-well production history matching of 45 wells and classical geostatistics . It is explored if this approach can be a proxy, with its limitations, to multi-well reservoir simulation. For this purpose, a reservoir model was built using available reservoir, well and production data to compare its results with those of the geostatistical maps for the same properties. Despite the expected local discrepancies due to differences between the two approaches, the results showed similar patterns and global distributions. Specific results showed that despite long-time operation of the wells in this area, there were still areas with high gas content and low gas effective permeability within the modeled time interval that might have benefited from further development using additional wells.

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Developing bare-earth digital elevation models from structure-from-motion data on barrier islands

Unoccupied aerial systems can collect aerial imagery that can be used to develop structure-from-motion products with a temporal resolution well-suited to monitoring dynamic barrier island environments. However, topographic data created using photogrammetric techniques such as structure-from-motion represent the surface elevation including the vegetation canopy . Additional processing is required for estimating bare-earth elevation, which is critical for understanding the underlying geomorphology of these islands. In this study, we used a vegetation and elevation survey to produce bare-earth digital elevation models from structure-from-motion-derived elevation products for two sites on Dauphin Island, Alabama (USA). One site was exposed to high wave energy and included a mix of beach, dune, and barrier flat habitats that were dominated by supratidal/upland herbaceous vegetation. The second site was exposed to low wave energy and was dominated by intertidal marsh. Aerial imagery was collected in late fall of 2018 and spring of 2019. We tested several machine learning algorithms for predicting and removing elevation bias for vegetated areas using predictors that included spectral indices from unoccupied aerial systems-based multispectral imagery and landscape position information (e.g., relative topography and distance from shore). Models were developed for each site and season. We also explored how well the model from one season generalized to data from a different season for the same site. For developing initial digital surface models, we found that utilizing a minimum bin algorithm, as opposed to interpolation, led to lower elevation bias. For bias removal, Gaussian process regression performed the best and led to a root mean square error for the bare-earth digital elevation models of around 0.10 m for the high energy site and 0.15 m for the low energy site. Compared to the digital surface models, the root mean square error for the bare-earth digital elevation models was reduced by at least 29 percent for the high energy site and 69 percent for the low energy site. For all models, common predictors included surface elevation, vegetation greenness, and distance from the shoreline. The models produced comparable results when trained using data from a different season. The error estimates for all analyses were within published elevation standards for lidar data for vegetated areas. With calibration, this approach could be portable to other areas or data, such as aerial lidar (conventional or unoccupied), to provide an efficient and repeatable framework for monitoring geomorphology or provide baseline elevations for predicting changes to these environments under future conditions.

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The influence of legacy contamination on the transport and bioaccumulation of mercury within the Mobile River Basin

Past industrial use and subsequent release of mercury (Hg) into the environment have resulted in severe cases of legacy contamination that still influence contemporary Hg levels in biota. While the bioaccumulation of legacy Hg is commonly assessed via concentration measurements within fish tissue, this practice becomes difficult in regions of high productivity and methylmercury (MeHg) production, like the Mobile River Basin, Alabama in the southeastern United States. This study applied Hg stable isotope tracers to distinguish legacy Hg from regional deposition sources in sediments, waters, and fish within the Mobile River. Sediments and waters displayed differences in δ 202 Hg between industrial and background sites, which corresponded to drastic differences in Hg concentration. Sites that were affected by legacy Hg, as defined by δ 202 Hg, produced largemouth bass with lower MeHg content (59–70%) than those captured in the main rivers (>85%). Direct measurements of Hg isotopes and mathematical estimates of MeHg isotope pools in fish displayed similar distinctions between legacy and watershed sources as observed in other matrices. These results indicate that legacy Hg can accumulate directly into fish tissue as the inorganic species and may also be available for methylation within contaminated zones decades after the initial release.

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