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Geology topics

W. A. Willford

Publications and source records attributed to W. A. Willford.

6 recordsLinked to original sources

The effect of anesthetization and urinary bladder catheterization on renal function of rainbow trout

1. Rainbow trout were anesthetized with MS-222 (Sandoz) or methylpentynol and catheterized. Urine was collected at selected intervals up to 48 hr. 2. Effects of MS-222 anesthesia on urine flow and composition were isolated from the stress of catheterization by re-anesthetizing the fish 18 to 20 hr post catheterization. 3. Urine output patterns were similar following MS-222 or methylpentynol anesthesia and catheterization. Highest urine flows were measured 4 to 8 hr post treatment. The highest urine output after re-anesthetization with MS-222 was observed 2 to 4 hr post-anesthesia. 4. Highest concentrations of Na 2+ , K + , Ca 2+ , Cl - and inorganic PO 4 in the urine were measured in the first 2 hr after anesthesia and catheterization. 5. Flow rates and chemical composition of urine indicate that "normal" renal function is re-established 12 to 24 hr post-treatment.

Comparative Biochemistry and Physiology

Chlorinated hydrocarbons in the young of Lake Michigan coho salmon

Three thousand eyed coho salmon (Oncorhynchus kisutch) eggs from Lake Michigan stock were sent by the Department of Natural Resources to the Fish Control Laboratory, La Crosse, Wis., on January 15, 1969, for use in evaluating candidate fish-cnotrol chemicals.

Progressive Fish-Culturist

Turnover and urinary excretion of free and acetylated MS-222 rainbow trout, Salmo gairdneri

Rainbow trout (Salmo gairdneri) anesthetized in 100 mg/liter of M.S. 222 at 12 C excreted the drug in free and acetylated forms via the urine during a 24-hr recovery period in freshwater. Of the M.S. 222 excreted, 77-96% was acetylated. Blood levels of free drug in anesthetized trout approximated 75% of the anesthetic concentration, but the amount of acetylated M.S. 222 was relatively insignificant. The blood and urine were cleared of the two fractions of M.S. 222 in 8 and 24 hr respectively. Low levels of aromatic amines of natural origin occurred in blood and urine and were subtracted from measurements of M.S. 222. Intraperitoneal injections of 10-100 mg/kg of M.S. 222 did not induce anesthesia; however, the 24-hr pattern of drug excretion was similar to that observed after anesthesia by immersion. Only 15-21 % of the injected dose was found in the urine, suggesting a second route of drug elimination.

Journal of the Fisheries Research Board of Canada