Evaluation of 215 Candidate Fungicides for Use in Fish Culture
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Geology topics
Publications and source records attributed to Tom A. Bailey.
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Four species of aquatic fungi ( Achlya flagellata, A. racemosa, Saprolegnia hypogyna , and S. megasperma ) were exposed to 25 chemicals representing seven classes of compounds for 15 and 60 min, in an effort to identify potential fungicidal agents for use in fish culture. The antifungal activity of each chemical was compared with that of malachite green, a reference compound with known fungicidal properties but not registered for fishery use. Six compounds which inhibited fungal growth on artificial media at concentrations of < 100 mg/l (listed in order of decreasing antifungal activity) were the cationics Du-ter ® and copper oxychloride sulfate, the amine Lesan ® , the amide BAS-389-O1F and the cationics Cuprimyxin ® and Roccal ® II. Certain chemicals from these classes of compounds may have promise as aquatic fungicides.
The reliability, reproducibility, and usefulness of three screening methods -- the cellophane transfer, the agar plug transfer, and the agar dilution -- to screen aquatic fungicides were evaluated. Achlya flagellata and Saprolegnia hypogyna were exposed to 1, 10, and 100 mg/L of malachite green to test each method. The cellophane transfer and agar plug transfer techniques had similar reliability and reproducibility in rating fungicidal activity, and were both superior to the agar dilution technique. The agar plug transfer and agar dilution techniques adequately projected in vivo activity of malachite green, but the cellophane transfer technique overestimated its activity. Overall, the agar plug transfer technique most accurately rated the activity of malachite green and was the easiest test to perform. It therefore appears to be the method of choice for testing aquatic fungicides.