Geology topics

Timothy M. Bartish

Publications and source records attributed to Timothy M. Bartish.

7 recordsLinked to original sources

USGS2007

Reproductive and Endocrine Biomarkers in Largemouth Bass (Micropterus salmoides) and Common Carp (Cyprinus carpio) from United States Waters

A nationwide reconnaissance investigation was initiated in 1994 to develop and evaluate a suite of reproductive and endocrine biomarkers for their potential to assess reproductive health and status in teleost (bony) fish. Fish collections were made at 119 sites, representing many regions of the country and land- and water-use settings. Collectively, this report will provide a national and regional benchmark and a basis for evaluating biomarkers of endocrine and reproductive function. Approximately 2,200 common carp (Cyprinus carpio) and 650 largemouth bass (Micropterus salmoides) were collected from 1994 through 1997. The suite of biomarkers used for these studies included: the plasma sex-steroid hormones, 17?-estradiol (E2) and 11 ketotestosterone (11KT); the ratio of E2 to 11KT (E2:11KT); plasma vitellogenin (VTG); and stage of gonadal development. This data report provides fish size, stage and reproductive biomarker data for individual fish and for site and regional summaries of these variables.

Data Series↗

USGS2007

Biomarker Benchmarks: Reproductive and Endocrine Biomarkers in Largemouth Bass and Common Carp from United States Waters

The U.S. Geological Survey (USGS) has developed a national database and report on endocrine and reproductive condition in two species of fish collected in U.S. streams and rivers. This information provides scientists with a national basis for comparing results of endocrine measurements in fish from individual sites throughout the country, so that scientists can better ascertain normal levels of biomarkers. The database includes information on several measures of reproductive and endocrine condition for common carp and largemouth bass. Data summaries are provided by reproductive season and geographic region. A national-scale reconnaissance investigation was initiated in 1994 by the USGS that utilized a suite of biological assays (biomarkers) as indicators of reproductive health, and potentially, endocrine disruption in two widely distributed species of teleost (bony) fish, largemouth bass (Micropterus salmoides) and common carp (Cyrinus carpio). The suite of assays included plasma sex-steroid hormones, stage of gonadal development, and plasma vitellogenin, an egg protein that indicates exposure to estrogenic compounds when found in male fish. More than 2,200 common carp and 650 largemouth bass were collected at 119 rivers and streams (fig. 1).

Fact Sheet↗

USGS2005

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants and their effects on fish in the Columbia River Basin

This project examined and analyzed 560 fish representing eight species from 16 sites in the Columbia River Basin (CRB) from September 1997 to April 1998. Ten of the 16 sampling locations were historical National Contaminant Biomonitoring Program (NCBP) sites where organochlorine and elemental contaminants in fish had been monitored from 1969 through 1986. Five sites were co-located at U.S. Geological Survey (USGS)-National Stream Quality Accounting Network (NASQAN) stations at which water quality is monitored. The sampling location at Marine Park in Vancouver, Washington did not correspond to either of the established monitoring programs. Eight of the sampling locations were located on the Columbia River; three were on the Snake River; two were on the Willamette River, and one site was on each of the Yakima, Salmon and Flathead Rivers. Common carp ( Cyprinus carpio ), black basses ( Micropterus sp. ), and largescale sucker ( Catostomus macrocheilus ) together accounted for 80% of the fish sampled during the study. Fish were weighed and measured then field-examined for external and internal lesions, and liver, spleen, and gonads were weighed to compute somatic indices. Selected tissues and fluids were obtained and preserved for analysis of fish health and reproductive biomarkers. Composite samples of whole fish from each station were grouped by species and gender and analyzed for persistent organic and inorganic contaminants and for dioxin-like activity using H4IIE rat hepatoma cell bioassay.

Columbia River Basin↗

USGS2004

Strategic plan for the U.S. Geological Survey status and trends of Biological Resources Program: 2004-2009

The mission of the USGS Status and Trends of Biological Resources Program is to measure, predict, assess, and report the status and trends of the Nation's biological resources to facilitate research, enable resource management and stewardship, and promote public understanding and appreciation of our living resources. Determining the status (abundance, distribution, productivity, and health) and trends (how these variables change over time) of our living natural resources is critical for their Trumpeter swan with numbered wing tags. This tech- protection or restoration. The Progg ran nique allows birds to he monitored remotely without the provides the USGS, other agencies of need for recapture to identify individuals. Photo by the Department of the Interior (DOI), Wayne Miller. other federal and state agencies, and the public with science-based monitoring data and information for local, regional, and national assessment of biological resources and the ecosystems that support them.

Circular↗

USGS2004

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants and their effects on fish in the Rio Grande Basin

We collected, examined, and analyzed 368 fish of seven species from 10 sites in the Rio Grande Basin (RGB) during late 1997 and early 1998. Four sites were National Contaminant Biomonitoring Program (NCBP) stations where organochlorine ad elemental contaminants in fish had been monitored from 1969 and through 1986. The other six were USGS-National Stream Quantity Accounting Network (NASQAN) stations where water quality is monitored. The objectives were to document temporal and geographic trends in the concentrations of accumulative organic and inorganic contaminants in RGB fish and the effects of contaminants on the fish; to continue testing the feasibility of incorporating biomarkers (that is, biochemical, histopathological, and other biological indicators of contaminant exposure or effects) into a monitoring program for large U.S. rivers; and to evaluate the compatibility of monitoring methods based on the analysis of fish with those used to monitor water by NASQAN. Common carp ( Cyprinus carpio ; carp) and black basses ( Micropterus sp.; bass) were the targeted species; together, they represented 77% of the fish collected. Each fish was examined in the field for externally and internally visible gross lesions, selected organs were weighed to compute various ponderal and organosomatic indices, and samples of tissues and fluids were obtained and preserved for analysis of fish health and reproductive biomarkers. Composite samples of whole fish from each station were grouped by species and gender and analyzed by instrumental methods for persistent organic and inorganic contaminants and for dioxin-like activity (TCDD-EQ) using the H4IIE rat hepatoma cell bioassay.

Rio Grande basin↗

USGS2004

Biomonitoring of Environmental Status and Trends (BEST) Program: Environmental contaminants and their effects on fish in the Yukon River Basin

This project collected, examined, and analyzed 217 fish representing three species at 10 stations in the U.S. portion of the Yukon River Basin (YRB) from May to October 2002. Four sampling sites were located on the Yukon River; two were located on the Porcupine River, and one site was on each of the Ray, Tanana, Tolavana, and Innoko Rivers. Norther pike ( Esox lucius ), longnose sucker ( Catostomus catostomus ), and burbot ( Lota lota ) were weighed and measured, and examined in the field for external and internal lesions, and liver, spleen, and gonads were weighed to compute somatic indices. Selected tissues and fluids were collected and preserved for analysis of fish health and reproductive biomarkers. Composite samples of whole fish from each station were grouped by species and gender and analyzed for organochlorines and elemental contaminants and for dioxin-like activity using H4IIE rat hepatoma cell bioassay.

Alaska↗

USGS1999

Biomonitoring of Environmental Status and Trends (BEST) Program: Field Procedures for Assessing the Exposure of Fish to Environmental Contaminants

This document describes procedures used to collect information, tissues, and fluids for documenting the exposure of fish to environmental contaminants. For the procedures described here, fish are captured (preferably by electrofishing) and held alive until processing (generally <1 h). Fish are weighed, measured, and examined for grossly visible external lesions and pathologies. A blood sample is collected by caudal veinipuncture using a needle and syringe. The fish is subdued and it's abdominal cavity opened. The internal organs are dissected from the fish for examination. The sex of the fish is determined by direct observation of its gonads. The liver is weighed (most species) and cut into small cubes and flash-frozen in cryogenic vials, which are stored and shipped in dry ice or liquid nitrogen. Additional liver cubes plus all grossly visible anomalies are preserved for histopathology. The gonads and spleen are weighed, and samples are preserved for histopathology. The kidneys are examined, and histopathology samples collected. A gill sample is also collected and preserved. All remaining tissues are returned to the carcass, which is wrapped in foil, labeled for chemical analysis, and chilled. Individual fish carcasses are composited by station, species, and gender; frozen; and shipped to the analytical laboratory. Procedures are also described for record keeping; processing blood to obtain serum and plasma; flash-freezing samples; cleaning equipment; and preventing the transport of living organisms among waterways. A list of necessary equipment and supplies is also provided.

Information and Technology Report↗