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Susan C. Ireland

Publications and source records attributed to Susan C. Ireland.

5 recordsLinked to original sources

Microhabitat use of native fishes in the Kootenai River: A fine‐scale evaluation of large‐scale habitat rehabilitation efforts

Fish and microhabitat data were collected at 542 prepositioned electrofishing sites (surface area of each site = 4 m 2 ) in the Kootenai River, Idaho, during 2014 and 2015 to evaluate small‐scale habitat use by fishes, as it relates to large‐scale habitat rehabilitation efforts. Samples were collected from a 12‐km braided segment of river that had received localized habitat rehabilitation treatments since 2011. Fish and microhabitat data were collected to investigate habitat drivers related to the occurrence and relative abundance of fishes. Each sampling location was selected at random and characterized as “treated” (i.e., rehabilitated) or “untreated” based on proximity to habitat treatments. Fishes sampled from backwaters composed 71% of the overall catch and 84% of the catch from locally untreated areas of the river. Species‐specific regression models suggested that water depth and current velocity influenced the occurrence and abundance of fishes. In particular, shallow habitats with low current velocities were important for small‐bodied native fishes and likely serve as important rearing areas for juvenile fish. These habitat conditions typically characterize backwater and channel‐margin habitats that are vulnerable to anthropogenic perturbation. Prioritizing process‐based rehabilitation of these areas in large, regulated rivers would allow natural channel‐forming processes for the benefit of native fishes.

Idaho

Comparison of a prepositioned areal electrofishing device and fixed underwater videography for sampling riverine fishes

Prepositioned areal electrofishing devices (PAEDs) are used to evaluate microhabitat use by fishes because they minimize fright biases associated with traditional electrofishing techniques (e.g., boat electrofishing). Similarly, fixed underwater videography (FUV) is commonly used to minimize the effect of observers on fish behavior. The specific objectives of this research were to evaluate estimates of taxonomic occurrence and diversity between PAEDs and FUV and determine an appropriate time interval between positioning and electrifying of a PAED to reduce effects of PAED positioning on fish occurrence. Video cameras were positioned instream at 28 locations on the Kootenai River, Idaho, prior to PAED deployment such that the entire immobilization zone of the PAED was captured on camera. Following a 4-min acclimation period, cameras recorded fish behavior approximately 15 min prior to and 20 min following PAED deployment. Electrical current was applied to the PAEDs for 20 s immediately following the FUV procedure, and immobilized fishes were collected and processed. Video footage was subsampled in the laboratory, and fishes in the video were identified and enumerated in 5-s or 20-s intervals. Fixed underwater videography sampled more taxa than PAEDs at any given site. However, fishes sampled with FUV were difficult to identify, and most individuals were classified as “unidentifiable.” Consequently, direct comparisons between FUV and PAEDs are limited. Our results indicate that PAEDs should remain undisturbed for a minimum of 12 min before the equipment is electrified. Both PAEDs and FUV provide an estimate of taxonomic occurrence, but logistical and financial constraints along with project objectives must be considered when selecting between these 2 gear types. Results from this study provide information on the effectiveness of each gear type as it relates to the characterization of riverine fish assemblages at a small spatial scale.

Western North American Naturalist

Electrofishing effort requirements for estimating species richness in the Kootenai River, Idaho

This study was conducted on the Kootenai River, Idaho to provide insight on sampling requirements to optimize future monitoring effort associated with the response of fish assemblages to habitat rehabilitation. Our objective was to define the electrofishing effort (m) needed to have a 95% probability of sampling 50, 75, and 100% of the observed species richness and to evaluate the relative influence of depth, velocity, and instream woody cover on sample size requirements. Sidechannel habitats required more sampling effort to achieve 75 and 100% of the total species richness than main-channel habitats. The sampling effort required to have a 95% probability of sampling 100% of the species richness was 1100 m for main-channel sites and 1400 m for side-channel sites. We hypothesized that the difference in sampling requirements between main- and side-channel habitats was largely due to differences in habitat characteristics and species richness between main- and side-channel habitats. In general, main-channel habitats had lower species richness than side-channel habitats. Habitat characteristics (i.e., depth, current velocity, and woody instream cover) were not related to sample size requirements. Our guidelines will improve sampling efficiency during monitoring effort in the Kootenai River and provide insight on sampling designs for other large western river systems where electrofishing is used to assess fish assemblages.

Idaho

Patterns of fish assemblage structure and habitat use among main- and side-channel environments in the lower Kootenai River, Idaho

The lower Kootenai River, Idaho, was sampled during the summers of 2012 and 2013 to evaluate its fish assemblage structure at seven sites within main- and side-channel habitats where large-scale habitat rehabilitation was undertaken. Understanding the current patterns of fish assemblage structure and their relationships with habitat is important for evaluating the effects of past and future rehabilitation projects on the river. Species-specific habitat associations were modeled, and the variables that best explained the occurrence and relative abundance of fish were identified in order to guide future habitat rehabilitation so that it benefits native species. The results indicated that the side-channel habitats supported higher species richness than the main-channel habitats and that nonnative fishes were closely associated with newly rehabilitated habitats. This research provides valuable insight on the current fish assemblages in the Kootenai River and the assemblage-level responses that may occur as a result of future rehabilitation activities.

Idaho

Establishment and partial characterization of a cell line from burbot Lota lota maculosa: susceptibility to IHNV, IPNV and VHSV.

This study describes the development and partial characterization of a continuous fibroblastic-like cell line (BEF-1) developed from late stage embryos of North American burbot Lota lota maculosa. This cell line has been maintained for over 5 yr and 100 passages in vitro. Cells were cultured using Eagle’s minimum essential medium with Earle’s salts (MEM) supplemented with GlutaMAX™, and 10% fetal bovine serum (FBS), pH 7.4. The addition of penicillin-streptomycin-neomycin (PSN) antibiotic mixture (0.05, 0.05, 0.1 mg ml–1, respectively) did not negatively influence cell replication; however, the antimycotic Fungizone™ (2.5 µg ml–1, amphotericin B) caused cell rounding and resulted in a severe decrease in cell proliferation. Optimal incubation temperature has been observed between 15 and 23°C, and at these temperatures cultures are routinely passed using standard trypsinization methods every 5 to 7 d at a split ratio of 1:3 or 1:4. The cell line was susceptible to isolates of the M and U North American genotypes of infectious hematopoietic necrosis virus (IHNV), and to isolates of genotypes I, IVa, and IVb of viral hemorrhagic septicemia virus (VHSV). In contrast, the cell line was refractory to infection by 2 North American isolates of infectious pancreatic necrosis virus (IPNV) from serotypes A1 and A9. This cell line provides a new laboratory tool, will allow further investigation into viral diseases of burbot and possibly other species, and is the first immortalized cell line reported from a species in the Gadidae (cod) family.

Diseases of Aquatic Organisms