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USGS2026

Ecology of reintroduced Rocky Mountain bighorn sheep in Dinosaur National Monument

Translocations have been widely used to restore and conserve bighorn sheep ( Ovis canadensis ) populations in North America. Some translocations have been successful, but many populations remain small and genetically isolated. Population structure can influence the viability and long-term success of reintroductions. Social ungulates often function as interconnected subpopulations (metapopulations); however, few studies evaluate subpopulation sizes, connectivity, and genetic diversity within metapopulations. To address this gap, we conducted a comprehensive study of a reintroduced Rocky Mountain bighorn sheep ( Ovis canadensis canadensis ) population in Dinosaur National Monument in Colorado and Utah, USA, between 2006–2020. We analyzed global positioning system (GPS) radio-collar data, genetic samples, and results of health testing to evaluate abundance, distribution, genetic structure and diversity, habitat use, movement and connectivity, and presence of or exposure to respiratory pathogens. We integrated these analyses to evaluate the outcomes of a reintroduction effort that began in 1952, over 70 years ago, and to inform management decisions in Dinosaur National Monument. We also provide a framework for evaluating metapopulation processes, including a non-invasive approach that links genetic structure with Bayesian spatial capture-recapture analyses to estimate subpopulation sizes. Despite models indicating continuous suitable habitat, we found a spatially structured population with at least 4 subpopulations with constrained connectivity. Evidence from step selection and density analyses suggested that movement among subpopulations may be limited by semi-permeable barriers including rivers and human disturbance, which could contribute to maintenance of spatial structure over time. In 2006, antibody to Mycoplasma ovipneumoniae was detected in all geographically and genetically distinct subpopulations. Widespread clinical signs of disease and a confirmed exposure to M. ovipneumoniae in 2019 indicate a long-term disease challenge. Proximity to domestic sheep creates repeated opportunities for introduction of new M. ovipneumoniae strains. We estimated abundance in 2019 at 109 (95% CrI = 87–133), composed of subpopulations ranging from 18–39 animals (95% CrIs from 11–50). Genetic diversity was relatively high compared to other reintroduced and native Rocky Mountain bighorn sheep populations, which is likely a consequence of multiple translocations from different sources. Three of 4 subpopulation centers generally aligned with the locations of original translocation release sites. Persistence in the presence of pathogens may be facilitated by metapopulation structure and moderately high genetic diversity. Conversely, metapopulation structure can also facilitate pathogen persistence. Our approach offers a path to advance understanding of the population ecology of reintroduced bighorn sheep and can inform effective conservation and management of their populations.

Colorado, Wyoming↗

USGS2025

Assessing diet and genotyping success of goat pellet surveys from 2019 in Glacier National Park

Fecal pellets contain genetic information and can be used to identify individuals, their diet, and more. Individual identification can be useful in understanding movements of individuals, developing population estimates, assessing vital rates, genetic diversity and structure, and evaluating trends over time (e.g., Epps et al 2024). Successful genotyping depends on the quality of the sample, which can be influenced by many things including the initial state of the sample, environmental conditions that the pellets were exposed to, and the method of storage. The diet of mountain goats ( Oreamnos americanus ) is hard to study as they are habitat specialists in some of the most remote and rugged alpine environments (Festa-Bianchet & Côté 2012). These iconic ungulates epitomize the renowned wilderness character of Glacier National Park (GNP), yet the population has declined by 45% since 2008 likely due at least in part to changing climate (Graves et al. 2025). This is well above the International Union for the Conservation of Nature’s population decline criterion used to designate a species as vulnerable (IUCN 2012). Mountain goats are known as dietary generalists employing highly variable diets across systems, but diets have not been identified for GNP. Identifying goat diet composition in GNP can provide the information needed to determine whether this population decline could be related to climate-change induced shifts in forage phenology, diversity, productivity, and plant community structure. Other anthropogenic changes in GNP, such as increases in exotic and invasive plant species, will continue to affect plant community composition (Lesica et al. 1993). These alterations may reduce optimal forage availability and quality, which could have a deleterious effect on mountain goat survival. To examine these possibilities, we must first ask- What precisely are the mountain goats of Glacier National Park eating? DNA metabarcoding of fecal pellets provides a cutting-edge, non-invasive method for assessing herbivore diet. Previous studies of mountain goat diet have used methods such as fecal crude protein analysis (Festa-Bianchet & Côté 2012), rumen analysis (Saunders 1955), fecal microhistology (Cobb et al. 2012), and bite-for-bite feeding observations (Daily et al. 1984). Many of these studies have only been able to identify diet components to functional plant type and the methods used are biased towards less digestible diet components such as grasses (McInnis et al. 1983). Metabarcoding of fecal pellets can provide a higher taxonomic resolution than previous methods (Scasta et al. 2019, Stapleton et al. 2022) and open the field for increased participation of volunteers, also called community or citizen scientists. Here, we use mountain goat fecal pellets to conduct 1) an evaluation of which conditions might influence genotyping success and 2) a preliminary assessment of diet using metabarcoding. This pilot study allowed us to evaluate which pellets to focus on collecting, identify the best storage methods, explore approaches for analyzing metabarcoding data, examine the utility of metabarcoding for acquiring taxonomically specific diet information, and to identify genetic sequences that could not be resolved to genus or species levels using these methods. We examined the frequency of occurrence and relative diet composition of identified forbs, shrubs, graminoids, mosses, and trees in mountain goat diets.

Montana↗