Geology topics

Robert Massatti

Publications and source records attributed to Robert Massatti.

At least 19 recordsLinked to original sources

USGS2026

Rethinking seed selection based on climate matching during restoration: Geography, soils and climate explain species-specific mortality

Implicit in the construction of seed transfer zones (STZs) are the assumptions that plant populations are adapted to their home climates and that transferring native seed across climate gradients risks maladaptation and poor performance. However, plants are adapted to multiple aspects of their environments that are often excluded from STZ development. Here, we used models integrating geographic distance, climate distance and soil metrics to predict plant mortality in an experimental garden for three restoration-relevant species in the southwestern United States: Bouteloua curtipendula , Heterotheca villosa and Sporobolus cryptandrus . Overall, climate distance explained mortality better than geographic distance, but increasing climate distance was not consistently associated with higher mortality. In contrast, mortality always increased with geographic distance. Species responded idiosyncratically to environmental gradients such as soil texture and pH, indicating that incorporating site-specific variables beyond climate can improve predictions of survival. Finally, seed sources of H. villosa from hotter, drier climates exhibited improved survival during abnormally hot, dry conditions at the experimental site, whereas no consistent pattern emerged for the two grass species. Collectively, our results suggest that seeding strategies extending beyond climate matching alone may better support restoration outcomes when species-specific guidance is unavailable.

Arizona, Colorado, New Mexico, Utah↗

USGS2025

Bridging theory and practice to inform seed selection for restoration

Introduction Land managers often face the critical decision of what plant materials to select for ecological restoration. Selection depends on factors that span ecological conditions and human interventions in the restoration process, which can strongly influence the recovery of a degraded ecosystem. Objectives To improve the seed selection process, we compared scientific literature and guidance from five federal agencies to bridge seed selection “theory” and “practice” in the interior western and central United States. Methods We extracted recurring themes and keywords associated with abiotic, biotic, land management, and producer practices that influence seed selection and analyzed their proportional use between agency guidance and scientific literature. Results We identified significant overlap between key concepts in guidance and literature, highlighting the interconnectedness between science and management. However, nuanced patterns emerged across seed selection themes, including more focus in the literature on local adaptation and genetic structure, ramifications of introducing gene flow and artificial selection during plant production, and provenancing strategies to address changing climatic conditions. In contrast, agency guidance focused on genetically appropriate and environmentally suitable seed selection and practical concerns, including seed cost, availability, and diversity. Seed selection studies in the literature were concentrated in the Great Basin and Colorado Plateau, scattered in the Great Plains, and were largely experimental. Conclusions Our review suggests new opportunities for co-production of science and decision-making frameworks among researchers, land managers, and seed producers to inform seed selection guidance for restoration.

Restoration Ecology↗

USGS2025

Suturing fragmented landscapes: Mosaic hybrid zones in plants may facilitate ecosystem resiliency

Many widespread plant taxa of western North America have diversified into phenotypically and genetically divergent lineages due to complex biogeographic histories across heterogeneous landscapes. Mosaic hybrid zones can form when geographically co-occurring, yet environmentally distinct, lineages cross-pollinate and form hybrids that occupy unique environmental niches absent of a geographic cline. This expands the total environmental space across which parental and hybrid individuals grow, resulting in larger, less fragmented geographic distributions. Here, we highlight hybridization mosaics across three study systems containing taxa critical to widespread plant communities in western North America: Ericameria nauseosa , Artemisia tridentata , and Sphaeralcea fendleri . The systems contain diverged taxa that co-occur across the landscape and hybridize readily. Hybridization among taxa has facilitated niche expansion into intermediate environments consistent with unique combinations of adaptive genetic variation, creating more continuity within each study system—study systems occupy ~820 to 270,000 km 2 more geographic area by virtue of hybridization. Furthermore, hybrids are predicted to play important roles in future climates, as they may occupy 8 to 475% larger distributions compared to present. Convergent patterns signal mosaic hybridization as an underappreciated mechanism with broad ecological and evolutionary ramifications. Leveraging mosaic hybridization may assist the creation of restoration management plans that aim to mitigate the deleterious effects of habitat fragmentation on ecosystems in the context of climate change.

western United States↗

USGS2025

Genetic structure of an expanding population of Humpback Chub in Grand Canyon

Objective Humpback Chub (HBC) Gila cypha in Grand Canyon declined in abundance and distribution over the latter part of the 20th century but have substantially increased in abundance and distribution over the past two decades. Although previous genetic work suggested that HBC in Grand Canyon belong to one genetic group, here we evaluate the genetic structure of HBC in Grand Canyon to determine whether relic populations in western Grand Canyon may have contributed unique variation to the recent population expansion or whether differences in behavior linked to migration in eastern Grand Canyon may promote assortative mating and heretofore unrecognized population structure. Methods Using fin clips collected from 167 individual HBC representing four sampling sites within Grand Canyon, we extracted DNA and developed data sets consisting of approximately 20,000 anonymous genomic loci. We quantified patterns of genetic diversity, and we accounted for outlier single-nucleotide polymorphisms to ensure that our interpretations of genetic patterns were not misled by adaptive processes and did not mask adaptively important genetic variation. Results Despite behavioral variation and the possibility that individuals with unique genetic variation survived in isolated warmwater sites within western Grand Canyon, HBC were not differentiated by sampling site or by differences in migratory behavior. Heterozygosity and nucleotide diversity were consistently high among sampling sites, and inbreeding coefficients were close to zero. Conclusions The HBC in Grand Canyon constitute a single genetic population. Our results do not preclude a genetic basis to migratory behavior, but our data suggest that this trait does not lead to assortative mating. Furthermore, while HBC may have survived in discontiguous warmwater refugia in western Grand Canyon during decades when the main stem was too cold for spawning, our data did not reveal any noticeable spatial variability in HBC genetics in the main stem after the recent HBC population expansion.

Arizona↗

USGS2024

The cost of self-defense: Browsing effects in the rare plant species Salix arizonica

Coevolution between plants and their animal predators has led to diverse defensive adaptations. Multiple theories of defense propose that there are resource allocation costs associated with producing chemical defenses. One leading hypothesis, optimal defense theory (ODT), suggests that natural selection will result in the allocation of resources to defenses that optimize the cost-to-benefit ratio between defense and other functional processes. The population decline of the rare subalpine wetland species, Arizona willow ( Salix arizonica ), has been attributed to various biotic and abiotic factors, with browsing from wild and domestic ungulates as a significant concern for at least three decades. In a field experiment using natural populations, we compare the relationship between phytochemical defense and height in Arizona willows with and without long-term protection from browsing via browse exclosures. Consistent with the predictions of ODT, individuals with physical protection from ungulate browsing for multiple years had significantly lower phenolic glycoside (PG) concentrations and increased plant height compared to unprotected individuals. A similar pattern was found across all individuals, whereby total PG concentration and height were negatively correlated. In a short-term experiment in natural populations, changes in levels of defense were not observed when plants received protection for only one growing season. The contrasting pattern of defense plasticity in response to long-term versus short-term physical protection suggests a differential plastic response in this long-lived species. Delayed reduction in PG concentration may serve as a benefit to avoid mismatches between environmental cues and responses. Our research sheds light on the intricate dynamics between plant-defense strategies, environmental pressures, and evolutionary adaptations in shaping plant–browser interactions.

Arizona, Colorado, New Mexico, Utah↗

USGS2024

Intraspecific trait variability in wild populations predicts neither variability nor performance in a common garden

Dryland restoration requires plant materials capable of performing well despite difficult growing conditions. Selecting plant materials with higher intraspecific trait variability (ITV) may support successful outcomes by enhancing the performance of those materials in restoration settings. However, maintaining ITV from wild populations is not well understood and requires further investigation if ITV is to be incorporated into native plant materials, which are often developed from wild-collected seed grown in agricultural settings. We used two perennial plant species to explore whether (1) ITV measured at field sites predicts ITV in a common garden, (2) rankings of ITV among populations remain stable over time, and (3) higher levels of ITV promote survival and reproductive effort in a common garden. We measured ITV in specific leaf area and height for Bouteloua curtipendula and Heterotheca villosa at field sites and over 2 years in a common garden, as well as survival and flower production in the common garden. We also calculated climate distance between field sites, where seeds were originally sourced, and the common garden to account for the impact of climatic differences on ITV. We found that (1) ITV measured at field sites did not predict ITV in the common garden, (2) rankings of ITV across populations were inconsistent, and (3) relationships between ITV and performance were rare and differed by species. Our findings indicate that the utility of ITV in wild populations as a predictive tool may be limited.

Restoration Ecology↗

USGS2023

Matching existing and future native plant materials to disturbance-driven restoration needs

Assessing the appropriateness of existing native plant materials can both determine which seed source to utilize for restoration projects, and identify locations for which new seed sources need to be developed. Here, we demonstrate an approach to meet these needs. This method identifies areas of high restoration need based on disturbance patterns, assesses the regional suitability of existing native plant materials based on climate similarity, and highlights geographic (and climatic) gaps where existing materials are likely unsuitable and where plant material development projects can be prioritized. We examined 12 high priority restoration species across the Colorado Plateau, a 38-million-ha region of the Intermountain West, United States to test our methodological pipeline. Fifty-four percent of the Colorado Plateau is disturbed by livestock grazing, wildfires that have burned in the past 20 years, or energy production from oil and gas wells, natural gas pipelines, and coal mines. Of the 28 commercially available plant materials for six of the focal species, only 3 have climate similarity that encompass more than 50% of the species modeled habitat on the Colorado Plateau. Across all commercial materials, most species (10 of 12) do not have any suitable plant material for 70% or more of their geographic range on the Colorado Plateau. Of those areas identified as not having any suitable plant materials, 47–56% are also disturbed. Our method provides usable, flexible protocols and spatially referenced data sources for optimizing the planning of new native plant materials in any region where restoration is needed and spatial data are available.

Arizona, Colorado, New Mexico, Utah↗

USGS2023

Supporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 2022 Report)

A primary focus of the Colorado Plateau Native Plant Program (CPNPP) is to identify and develop appropriate native plant materials (NPMs) for current and future restoration projects. Multiple efforts have characterized the myriad challenges inherent in providing appropriate seed resources to enable effective, widespread restoration and have identified a broad suite of research activities to provide the information necessary to overcome those challenges (e.g., Plant Conservation Alliance, 2015; Breed et al., 2018; Winkler et al., 2018; NASEM, 2023). Many of the most complex information needs relate to identifying the appropriate sources of plant species that can successfully establish in dryland environments, like the Colorado Plateau, where low and highly variable precipitation is standard. Providing this information requires synergistic research efforts in which results from earlier investigations inform the design of subsequent investigations. The U.S. Geological Survey (USGS) Southwest Biological Science Center’s (SBSC’s) research activities to support CPNPP in FY22 followed the FY22 Statement of Work to support a research framework that is continually adapting based on the needs of the restoration community and results from previous investigations; the long-term research framework is outlined in the 2019-2023 5-Year Research Strategy (hereafter referred to as the 5-year plan). This research framework provides support for the National Seed Strategy for Rehabilitation and Restoration (Plant Conservation Alliance, 2015), Biden-Harris Administration Executive Order 14008 (Tackling the Climate Crisis at Home and Abroad), and Department of Interior Priority #4 (Working to conserve at least 30% each of our lands and waters by the year 2030). Research activities in FY22 centered on landscape genomics, monitoring common garden and seeding experiments near Vernal and Moab, UT, conducting experimental treatments using the GRID (Germination for Restoration Information and Decision-making) framework, and continuing newer genetics projects to investigate the impact of productions techniques on plant materials and restoration treatments on native plant communities. These activities were supported by two biological science technicians. The effects of the SARS-CoV-2 pandemic were less visible in FY22, allowing SBSC to catch up on backlogged laboratory work. The overall progress of SBSC’s research remains on track with respect to the 5-year plan. While Dr. Rob Massatti was the only scientist supported by the SBSC-CPNPP agreement in FY22, other scientists, including Drs. John Bradford, Seth Munson, Mike Duniway, Sasha Reed, and Daniel Winkler, spent a considerable amount of time providing expertise and support for individual projects. Work activities performed in support of each 5-year plan goal are discussed in turn.

Colorado Plateau↗

USGS2022

Evolutionary dynamics inform management interventions of a hanging garden obligate, Carex specuicola

Uncovering the historical and contemporary processes shaping rare species with complex distributions is of growing importance due to threats such as habitat destruction and climate change. Species restricted to specialized, patchy habitat may persist by virtue of life history characteristics facilitating ongoing gene flow and dispersal, but they could also reflect the remnants of formerly widespread, suitable habitat that existed during past climate regimes. If formerly widespread species did not rely upon traits facilitating high dispersibility to persist, contemporary populations could be at high risk of extirpation or extinction. Fortunately, genomic investigations provide an opportunity to illuminate such alternative scenarios while simultaneously offering guidance for future management interventions. Herein, we test the role of these mechanisms in shaping patterns of genomic diversity and differentiation across a highly restricted and rare ecosystem: desert hanging gardens. We focus on Carex specuicola (Cyperaceae), a hanging garden obligate narrowly distributed in the Four Corners region of the southwestern United States that is listed as Threatened under the United States Endangered Species Act. Population structure and diversity analyses reveal that hanging garden populations are shaped by strong genetic drift, but that individuals in gardens are occasionally more closely related to individuals at other gardens than to individuals within the same garden. Similarly, gardens separated by long geographic distances may contain individuals that are more closely related compared to individuals in gardens separated by short geographic distances. Demographic modeling supports historical gene flow between some contemporary garden pairs, which is corroborated by low estimates of inbreeding coefficients and recent divergence times. As such, multiple lines of evidence support dispersal and gene flow across C. specuicola populations at both small and large spatial scales, indicating that even if C. specuicola was formerly more widespread, it may be well suited to persist in hanging gardens so long as suitable habitat remains available. Analyses like those demonstrated herein may be broadly applicable for understanding the short- and long-term evolutionary processes influencing rare species, and especially those having complex distributions across heterogeneous landscapes.

Arizona, Utah↗

USGS2022

Assessing population genomic structure and polyploidy: A crucial step for native plant restoration

Establishing an effective restoration program requires baseline genetic information to make sound decisions for seed increase and transfer. For many plants this information is lacking, especially among native forbs that are critical for pollinator health. Erigeron speciosus is a widespread, perennial forb occupying montane environments in the western United States and Canada. This species is important in fostering pollinator diversity. Our study examines the population genetic patterns across the species range using reduced-representation sequencing and surveys for genome duplication using flow cytometry and cytology. These genomic tools provide critical information for seed increase and seed transfer, necessary for restoration programs. Population genetic differentiation ( F ST ) average was 0.13 and ranged from 0.05 to 0.24 among 23 collection sites. Model-based Bayesian clustering supported a model with collection sites grouped into two populations, occupying distinct geographic regions of this species range. A genetic distance-based neighbor-joining tree also supported this division. Flow cytometry of 53 samples from 17 populations had 2C values that ranged from 1.7 to 3.6 pg with a mean 2C value of 2.3 pg. Putative triploids were found in two individuals from one collection site. The spatial distribution of genetic structure supports regionally based taxonomic descriptions of two varieties: speciosus in the North and macranthus in the South. This assessment of genetic structure and genome duplication describes an effective approach in developing baseline genetic information for restoration species, especially those species that may harbor complex taxonomy and polyploidy.

Colorado, Idaho, Montana, Oregon, South Dakota, Ut↗

USGS2022

Spatially explicit management of genetic diversity using ancestry probability surfaces

1. Ecological restoration and conservation efforts are increasing worldwide and the management of intraspecific genetic variation in plants and animals, an important component of biodiversity, is increasingly valued. As a result, tailorable, spatially explicit approaches to map genetic variation are needed to support decision-making and management frameworks related to the recovery of threatened and endangered species and the maintenance of genetic resources in species utilized by humans, such as for restoration or agricultural purposes. 2. Here, we describe and demonstrate a workflow to spatially interpolate patterns of genetic differentiation using novel functions in the R package POPMAPS ( Pop ulation M anagement using A ncestry P robability S urfaces). Our approach uses empirical genetic data to estimate ancestry coefficients across a user-defined landscape correlated with patterns of differentiation in the focal species. The resulting surface, which we term the ancestry probability surface, includes two components: hard population boundaries and estimations of uncertainty that represent confidence in population assignments (i.e., ancestry probabilities). 3. An ancestry probability surface developed for Hilaria jamesii , an important graminoid utilized in restoration across the western United States, demonstrates the functionality of POPMAPS . Genetic distances among empirical sites correlated better with least-cost distances across suitable habitat than with geographic distances, informing the surface over which the interpolation was conducted (i.e., a model indicating habitat suitability). A jackknifing procedure identified parameter values resulting in robust population assignments across the species’ range, which were utilized in downstream analyses to estimate ancestry coefficients from empirical data. Ancestry coefficients were translated into ancestry probabilities, which tended to be low for cells that were intermediate in distance between empirical sampling locations representing different populations or when influenced by empirical sampling locations with mixed genetic ancestry. 4. POPMAPS allows users to tailor parameter values and analytical approaches and thereby incorporate species-specific biological characteristics and desired levels of uncertainty into maps illustrating patterns of genetic differentiation. Ancestry probability surfaces may be used to guide management or investigate further ecological or evolutionary hypotheses. We discuss how maps produced by POPMAPS can inform multiple management challenges including species recovery planning and the utilization of commonly used species in restoration.

Methods in Ecology and Evolution↗

USGS2022

Hybrid enrichment of adaptive variation revealed by genotype-environment associations in montane sedges

The role of hybridization in diversification is complex and may result in many possible outcomes. Not only can hybridization produce new lineages, but those lineages may contain unique combinations of adaptive genetic variation derived from parental taxa that allow hybrid-origin lineages to occupy unique environmental space relative to one (or both) parents. We document such a case of hybridization between two sedge species, Carex nova and Carex nelsonii (Cyperaceae), that occupy partially overlapping environmental space in the southern Rocky Mountains, USA. In the region hypothesized to be the origin of the hybrid lineage, one parental taxon ( C. nelsonii ) is at the edge of its environmental tolerance. Hybrid-origin individuals display mixed ancestry between the parental taxa – of nearly 7,000 unlinked loci sampled, almost 30% showed evidence of excess ancestry from one parental lineage – approximately half displayed a genomic background skewed towards one parent, and half skewed towards the other. To test whether excess ancestry loci may have conferred an adaptive advantage to the hybrid-origin lineage, we conducted genotype-environment association analyses on different combinations of loci – with and without excess ancestry – and with multiple contrasts between the hybrids and parental taxa. Loci with skewed ancestry showed significant environmental associations distinguishing the hybrid lineage from one parent ( C. nelsonii ), whereas loci with relatively equal representation of parental ancestries showed no such environmental associations. Moreover, the overwhelming majority of candidate adaptive loci with respect to environmental gradients also had excess ancestry from a parental lineage, implying these loci have facilitated the persistence of the hybrid lineage in an environment unsuitable to at least one parent .

Arizona, Colorado, Idaho, Kansas, Montana, Nebrask↗

USGS2022

Supporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 2021 Report)

A primary focus of the Colorado Plateau Native Plant Program (CPNPP) is to identify and develop appropriate native plant materials (NPMs) for current and future restoration projects. Multiple efforts have characterized the myriad challenges inherent in providing appropriate seed resources to enable effective, widespread restoration and have identified a broad suite of research activities to provide the information necessary to overcome those challenges (e.g., Plant Conservation Alliance 2015; Breed et al. 2018; Winkler et al. 2018; McCormick et al. 2021). Many of the most complex information needs relate to identifying the appropriate sources of plant species that can successfully establish in dryland environments, like the Colorado Plateau, where low and highly variable precipitation is standard. Providing this information requires synergistic research efforts in which results from earlier investigations inform the design of subsequent investigations. The U.S. Geological Survey (USGS) Southwest Biological Science Center’s (SBSC’s) research activities to support CPNPP in FY21 followed the FY21 Statement of Work to support a research framework that is continually adapting based on the needs of the restoration community and results from previous investigations; the long-term research framework is outlined in the 2019-2023 5-Year Research Strategy (hereafter referred to as the 5-year plan). This research framework provides support for the National Seed Strategy for Rehabilitation and Restoration (Plant Conservation Alliance 2015), Biden-Harris Administration Executive Order 14008 (Tackling the Climate Crisis at Home and Abroad), and Department of Interior Priority #4 (Working to conserve at least 30% each of our lands and waters by the year 2030). Research activities in FY21 centered on landscape genomics, implementing and monitoring a common garden experiment near Vernal, UT, conducting experimental treatments using the GRID (Germination for Restoration Information and Decision-making) framework, and initiating new genetics projects to investigate the impact of production techniques on plant materials and restoration treatments on native plant communities. These activities were supported by four biological science technicians. The SARS-CoV-2 pandemic delayed some aspects of the FY21 workplan, especially for outside laboratory services. However, goals were largely met, and the overall progress of research remains on track with respect to the 5-year plan. While Dr. Rob Massatti was the only scientist supported by the SBSC-CPNPP agreement in FY21, other scientists, including Drs. John Bradford, Seth Munson, Mike Duniway, Sasha Reed, and Daniel Winkler, spent a considerable amount of time providing expertise and support for individual projects. Work activities performed in support of each 5-year plan goal are discussed in turn. Products resulting from FY21 research activities are reported in Appendix 1.

Arizona, Colorado, New Mexico, Utah↗

USGS2021

How to increase the supply of native seed to improve restoration success: The US native seed development process

With the United Nations Decade on Ecosystem Restoration, restoration of damaged ecosystems is turning into a global movement. Restoration actions that are not based on science and an understanding of ecosystem function can thwart desired restoration outcomes at best and cause further damage to ecosystems at worst. Restoration often includes revegetation using seed. Where we source seed for restoration can make a difference for species establishment, restoration outcomes, and recovery of ecosystem function. However, sourcing seeds of native species, let alone genetically appropriate seed, is not currently possible for many restoration projects. The process of increasing and sourcing suitable seed for restoration includes many steps that need to be addressed typically years before a restoration project is initiated. These steps of seed collection, evaluation and development, field establishment, production, certification and procurement, storage, and finally restoration, need to be considered ideally at a scale larger than individual restoration projects and with research conducted in each step. We describe these steps as implemented in the United States, the challenges therein, and provide suggestions and examples of how groups can make efficient and effective progress toward getting the right seed in the right place at the right time.

Restoration Ecology↗

USGS2021

Demographic modeling informs functional connectivity and management interventions in Graham’s beardtongue

Functional connectivity (i.e., the movement of individuals across a landscape) is essential for the maintenance of genetic variation and persistence of rare species. However, illuminating the processes influencing functional connectivity and ultimately translating this knowledge into management practice remains a fundamental challenge. Here, we combine various population structure analyses with pairwise, population-specific demographic modeling to investigate historical functional connectivity in Graham’s beardtongue ( Penstemon grahamii ), a rare plant narrowly distributed across a dryland region of the western US. While principal component and population structure analyses indicated an isolation-by-distance pattern of differentiation across the species’ range, spatial inferences of effective migration exposed an abrupt shift in population ancestry near the range center. To understand these seemingly conflicting patterns, we tested various models of historical gene flow and found evidence for recent admixture (~ 3400 generations ago) between populations near the range center. This historical perspective reconciles population structure patterns and suggests management efforts should focus on maintaining connectivity between these previously isolated lineages to promote the ongoing transfer of genetic variation. Beyond providing species-specific knowledge to inform management options, our study highlights how understanding demographic history may be critical to guide conservation efforts when interpreting population genetic patterns and inferring functional connectivity.

Conservation Genetics↗

USGS2021

The demographic and ecological factors shaping diversification among rare Astragalus species

Aim Evolutionary radiations are central to the origin and maintenance of biodiversity, yet we rarely understand how they are jointly shaped by demography and ecological opportunity. Astragalus is the largest plant genus in the world and is disproportionately comprised of rare species restricted to narrow geographic and ecological regions. Here, we explored the demographic and ecological mechanisms underlying patterns of diversification in a threatened Astragalus species complex endemic to a small desert region in the western United States. Location Southeast Utah, USA. Methods We used high‐throughput DNA sequencing to infer genetic structure, genetic diversity, and demographic history (i.e., the timing of population divergence, effective population sizes and gene flow) among Astragalus taxa. We performed landscape genetic analyses to quantify the relationships between genetic differentiation, geographic distance, and ecological distance based on bioclimatic and soil variables. Finally, we identified putative adaptive loci that show higher genetic differentiation between taxa than expected based on our inferred neutral demographic model. Results We found evidence of low gene flow between three highly differentiated taxa (currently delineated as A . iselyi , A . sabulosus var. sabulosus and A. sabulosus var. vehiculus ) that rapidly diverged from a small ancestral population near the beginning of the last glacial period. Genomic signatures revealed long‐term effective population sizes are 2–10× larger than recent census sizes, perhaps due to the maintenance of standing genetic variation through seed banks. Consistent with limited dispersal and local adaptation, genome‐wide patterns of differentiation are shaped by geographic distance (isolation‐by‐distance) and climate and soil variation (isolation‐by‐environment). Taxon‐specific adaptation is further supported by uncovering putative adaptive loci. Main Conclusions Our findings suggest that interactions between demography (i.e., dispersal limitations and seeds banks) and ecological opportunity (i.e., spatial and temporal environmental heterogeneity) may promote diversification, endemism, and rarity among closely related Astragalus species and similar plant clades distributed across complex landscapes.

Utah↗

USGS2021

Testing which axes of species differentiation underlie covariance of phylogeographic similarity among montane sedge species

Co‐distributed species may exhibit similar phylogeographic patterns due to shared environmental factors or discordant patterns attributed to the influence of species‐specific traits. Although either concordant or discordant patterns could occur due to chance, stark differences in key traits (e.g., dispersal ability) may readily explain differences between species. Multiple species’ attributes may affect genetic patterns, and it is difficult to isolate the contribution of each. Here we compare the relative importance of two attributes, range size and niche breadth, in shaping the spatial structure of genetic variation in four sedge species (genus Carex ) from the Rocky Mountains. Within two pairs of co‐distributed species, one species exhibits narrow niche breadth, while the other species has broad niche breadth. Furthermore, one pair of co‐distributed species has a large geographical distribution, while the other has a small distribution. The four species represent a natural experiment to tease apart how these attributes (i.e., range size and niche breadth) affect phylogeographic patterns. Investigations of genetic variation and structure revealed that range size, but not niche breadth, is related to spatial genetic covariation across species of montane sedges. Our study highlights how isolating key attributes across multiple species can inform their impact on processes driving intraspecific differentiation.

Molecular Ecology↗

USGS2021

Supporting the development and use of native plant materials for restoration on the Colorado Plateau (Fiscal Year 2020 Report)

A primary focus of the Colorado Plateau Native Plant Program (CPNPP) is to identify and develop appropriate native plant materials (NPMs) for current and future restoration projects. Multiple efforts have characterized the myriad challenges inherent in providing appropriate seed resources to enable effective, widespread restoration and have identified a broad suite of research activities to provide the information necessary to overcome those challenges (e.g., Plant Conservation Alliance 2015; Breed et al. 2018; Winkler et al. 2018). Many of the most complex information needs relate to identifying the appropriate sources of plant species that can successfully establish in dryland environments, like the Colorado Plateau, where precipitation is generally low and highly variable. Providing this information requires synergistic research efforts that build upon insight gained from earlier investigations. The U.S. Geological Survey (USGS) Southwest Biological Science Center’s (SBSC’s) research activities that supported CPNPP in FY20 followed the FY20 Statement of Work to support a research framework that is continually adapting based on the needs of the restoration community and results from previous investigations; the long-term research framework is outlined in the 2019-2023 5-Year Research Strategy (hereafter referred to as the 5-year plan). This research framework provides support for the National Seed Strategy for Rehabilitation and Restoration (Plant Conservation Alliance, 2015), Department of Interior Secretarial Order #3347 ( Conservation Stewardship and Outdoor Recreation ), and Bureau of Land Management Leadership Priority #1 ( Create a conservation stewardship legacy second only to Teddy Roosevelt ). Research activities in FY20 centered on landscape genomics, implementing a common garden experiment near Vernal, UT, conducting experimental treatments using the GRID (Germination for Restoration Information and Decision-making) framework, and collecting seeds and leaf tissues in the field in preparation for future experiments. These activities were supported by five biological science technicians. The SARS-CoV-2 pandemic delayed some aspects of the FY20 workplan, especially for contract laboratory services and the construction of the GRID garden in Flagstaff, AZ. However, goals were largely met, and the overall progress of research remains on track with respect to the 5-year plan. While Dr. Rob Massatti was the only scientist supported by the SBSC-CPNPP agreement in FY20, other scientists, including Drs. John Bradford, Seth Munson, Mike Duniway, Sasha Reed, Matt Jones, and Daniel Winkler, spent a considerable amount of time providing expertise and support for individual projects. Work activities performed in support of each goal are discussed in turn.

Arizona, Colorado, New Mexico, Utah↗