Geology topics

Rafael O. Tinoco

Publications and source records attributed to Rafael O. Tinoco.

9 recordsLinked to original sources

USGS2026

Controlling invasive carp ichthyoplankton dispersion using a streamwise-oriented bubble screen: A proof-of-concept validation in a laboratory flume

Recent evidence of invasive grass carp ( Ctenopharyngodon idella ) reproducing in tributaries to the Laurentian Great Lakes has highlighted the need for control efforts targeting multiple life stages. Initial attempts to control dispersal of downstream-drifting invasive carp ichthyoplankton (i.e., eggs and larvae) using an oblique bubble screen (OBS) revealed that nearly neutrally buoyant grass carp eggs and larvae enter helical-like motions driven by the OBS, preventing aggregation within a single capture location. To improve dispersal control methods for such early-life stage carp, we used a laboratory flume to investigate the efficacy of a streamwise-oriented bubble screen to facilitate their near-bank capture. Five early-life stages of grass carp were tested: live water-hardened eggs, pre- and post-gas bladder inflation larvae, dead larvae, and dead eggs (preserved in formalin solution and later rehydrated). A range of mean channel velocities (0.23, 0.45, and 0.75 m/s) was tested for all drifters. Capture percentages increased with increasing airflow. Preserved eggs, for instance, showed capture percentages up to 95 %, 87 %, and 69 % at low, medium, and high water velocities for the highest airflow rate, respectively, in contrast with the lower than 5 % capture measured for zero airflow cases. Symmetric secondary flow structures on either side of the bubble screen induced helical trajectories of drifters and facilitated their capture in net-arrays along each wall. Velocity data were used to estimate helical recirculation timescales, enabling calculation of optimal bubble diffuser and net-array lengths for desired capture rates. This study provides useful guidance for the design of effective systems to control dispersal of downstream-drifting ichthyoplankton of invasive carp in streams.

Journal of Great Lakes Research↗

USGS2026

Development and assessment of fluorescent-dyed, preserved invasive grass carp (Ctenopharyngodon idella) eggs as surrogates for live eggs in transport and dispersal control experiments

Invasive species such as grass carp ( Ctenopharyngodon idella ) pose substantial ecological threats to North American freshwater ecosystems. Understanding their early life stage behavior is critical for management efforts. From spawning to hatching, invasive carp eggs must remain suspended in the water column while drifting downstream for the best chance of survival. This highly vulnerable life stage is a potential target for population control to reduce recruitment. However, studying egg transport and potential dispersal control techniques is challenging, because the availability of live eggs and time period for experimentation are extremely limited. Additionally, accurately replicating the physical characteristics and transport mechanisms of fish eggs using surrogates in laboratory and field studies is not trivial. This study presents a novel method to create fluorescein-dyed, preserved grass carp eggs as surrogates for live eggs in transport and dispersal control experiments. This technique enables year-round studies of grass carp egg transport, offering managers a reliable tool for developing and testing dispersal control and passive sampling methods for invasive carp eggs. In this study, we rehydrate and dye preserved grass carp eggs in varying concentrations of aqueous fluorescein for a range of rehydration times, evaluate dye retention and egg visibility under ultraviolet light (UV-A), and measure diameters and settling velocities for comparison with live eggs. Eggs rehydrated in 0.100 g per liter fluorescein for 30 min maintain adequate brightness for up to 40 min in mixed conditions and exhibit mean settling velocities and densities similar to live eggs, making them ideal for laboratory experiments using quantitative imaging techniques.

River Research and Applications↗

USGS2025

Efficacy of oblique bubble screen deterrents on redirecting live eggs and larvae of grass carp (Ctenopharyngodon idella) at different developmental stages

Invasive carp have severely damaged aquatic ecosystems in the USA, particularly in the Mississippi River Basin. Behavioral deterrents have been developed in the last few decades to control population expansion into new ecosystems. However, none of these deterrents are capable of controlling early-life stage carp, which have limited or no mobility during their drifting stage in rivers. Capturing eggs and larvae in large numbers warrants new methods due to their distinct physical and biological properties as well as their lack of behavioral response. We tested a novel method to redirect downstream drifting eggs and larvae for their efficient removal in streams using an oblique bubble screen (OBS) deterrent. We investigated the effects of mean water velocity and airflow rate on redirection of eggs, pre-gas bladder inflation (GBI) larvae, near-GBI larvae, and dead larvae. Although similar OBS configurations had shown high efficacy redirecting plastic spheres (egg surrogates) in previous studies, they underperform redirecting live eggs and larvae. However, distinct patterns were identified for eggs, pre-GBI, near-GBI, and dead larvae. A detailed hydrodynamic analysis showed that eggs closely follow the larger scales of motions created by the OBS, and that larvae can actively respond to turbulence cues. This study yielded new insights into the movement of early-life stage grass carp in a turbulent flow with strong recirculation, and provided important data to improve the design of bubble screen dispersal barriers for invasive carp management and population control.

Water Resources Research↗

USGS2024

Limiting downstream dispersal of invasive carp egg surrogates using a laboratory-scale oblique bubble screen

We conceptualize and test a non-intrusive barrier, comprised of an oblique bubble screen (OBS) oriented at an angle to the mean flow, to prevent the downstream dispersal of invasive carp egg surrogates. Three surrogates of different densities and diameters were tested. Secondary flows created by the OBS were tuned to redirect surrogate eggs to facilitate their capture. Surface particle image velocimetry and acoustic Doppler velocimetry were used to characterize secondary flows. We assessed the influence of airflow rate, OBS angle, mean flow velocity, and surrogate density on particle redirection. In general, redirection efficiency improves by increasing the OBS angle with respect to the cross-section. At a mean flow velocity of 0.75 metres per second (m/s), the OBS system redirected up to 60% (%) of positively buoyant particles (specific gravity SG = 0.9, and diameter d = 7.09 millimetres [mm]) and 40% of semi-buoyant particles (SG = 1.001, d = 3.1 mm). Negatively buoyant particles (SG = 1.04, and d = 5.90 mm) were redirected by the physical structure of the diffuser rather than by OBS-induced flow. The study shows that an OBS system can be used to effectively redirect carp-egg surrogates over a wide range of particle sizes and densities, allowing for selective targeting of undesired particles in streams.

Journal of Ecohydraulics↗

USGS2022

Assessing the efficacy of oblique bubble screens for control of aquatic invasive species

Non-physical barriers, such as bubble screens (or curtains), are promising low-impact strategies to deter the spread of Aquatic Invasive Species (AIS) in streams. Bubble screens have been successfully implemented to redirect and/or deter adult fish and to capture plastics in some rivers, but their efficacy on invasive fish at multiple life stages (eggs, larvae, and adult fish) is not yet known. Air bubbles rising from a diffuser placed at the bottom of a stream generate counterrotating eddies that interact with the mean flow. Understanding such interactions allows us to design an Oblique Bubble Screen (OBS), a system able to direct drifting particles (i.e., eggs and larvae) towards the banks of a stream for potential capture, based on the water velocity, river morphology, orientation of the OBS, diffuser material, and air flow rate. We present the results from a series of laboratory experiments at the Ecohydraulics and Ecomorphodynamics Laboratory at the University of Illinois at Urbana-Champaign, using positively buoyant (specific gravity SG=0.9, and diameter d=7.09mm) and negatively buoyant (SG=1.04, d=5.9mm) spherical particles to represent the range of size and density of developing Grass carp eggs, an invasive species in North America (Ctenopharyngodon idella). An air compressor was connected to a porous tube laid at the bottom of a recirculating flume, with a regulator and a flow meter to control air pressure and air flow rate. Acoustic Doppler Velocimeters (ADV) and Surface Particle Image Velocimetry (PIV) were used to measure the effect of the OBS on the velocity field. Our collected data showed that: (1) a single OBS is able to direct drifting particles towards the bank at the downstream end of the OBS, (2) adjusting orientation angle and air flow rate of the diffuser can increase capture efficacy under different flow conditions, reaching up to a 100% of capture for buoyant particles, and (3) the ratio between lateral velocity generated by the OBS (as a function of air flow rate) and the mean longitudinal flow velocities, can be used as an indicator to predict whether the OBS will be able to carry the particles all along the length of the diffuser in wider and deeper streams. The optimal configurations from our study will be tested with live Grass carp eggs and larvae, as well as with upstream-swimming adult carp to assess its potential as a two-way barrier, and to provide design parameters to set the air-flow rate and diffuser type needed for field deployments, according to width-to-depth ratios and stream morphology.

Conference Paper↗

USGS2021

Using turbulence to identify preferential areas for grass carp (Ctenopharyngodon idella) larvae in streams: A laboratory study

In this experimental series, we studied the swimming capabilities and response of grass carp ( Ctenopharyngodon idella ) larvae to flow turbulence in a laboratory flume. We compared three different experimental configurations, representing in‐stream obstructions commonly found in natural streams (e.g., a gravel bump, a single vertical cylinder, and patches of submerged rigid vegetation). Grass carp larvae (postgas bladder emergence) were introduced to each experimental configuration and subjected to a variety of hydrodynamic forces of different magnitudes and scales. We varied the flow velocities and water depths and found ranges of turbulent kinetic energy and Reynolds stresses that triggered a response in larval trajectories, identified by measured horizontal and vertical swimming speeds for each flow condition. Larvae apparently actively avoided areas with increased levels of turbulence by swimming away, moving faster in short bursts, and expending more energy. In addition to the magnitude of turbulent kinetic energy, the length scale and time scale of turbulent eddies also influenced the larvae response. These findings support the development of new strategies for controlling the spread of grass carp larvae in rivers, as well as the development of numerical tools incorporating active swimming capabilities to predict larval transport in streams.

Water Resources Research↗

USGS2020

Identifying turbulence features hindering swimming capabilities of grass carp larvae (Ctenopharyngodon idella) through submerged vegetation

Aquatic vegetation can provide habitat and refuge for a variety of species in streams. However, the flow features generated by submerged patches of vegetation can also pose a challenge for fish larvae. We conducted a series of experiments with live grass carp larvae (starting ∼50 h post hatch) in a laboratory racetrack flume, using a submerged array of rigid cylinders to mimic vegetation. We used particle image velocimetry to characterize the flow field, and particle tracking velocimetry to obtain position and displacement of the fish. Four speeds and two submergence ratios were investigated. In contrast with previous studies with grass carp eggs, our data showed an active response from larvae to determine their position. Our study shows that: (1) mean velocity by itself is not a reliable predictor, as some larvae will seemingly prefer to be in areas of higher speeds with lower shear and turbulence, (2) turbulence characteristics can be used to identify areas avoided by larvae, (3) turbulence length scales are relevant to determine spatial distribution of larvae and their swimming capabilities within and above vegetated patches and similar roughness elements in streams. These findings can inform the design of monitoring and control strategies in rivers based on turbulence and turbulence scales generated by natural and man-made instream structures.

Journal of Ecohydraulics↗

USGS2019

Influence of turbulence and in-stream structures on the transport and survival of grass carp eggs and larvae at various developmental stages

Understanding the response of grass carp to flow and turbulence regimes during early life stages is fundamental to monitoring and controlling their spread. A comprehensive set of hydrodynamic experiments was conducted with live grass carp eggs and larvae, to better understand their drifting and swimming patterns with 3 different in-stream obstructions: (1) a gravel bump, (2) a single cylinder, and (3) submerged vegetation. The hydrodynamic behavior of eggs and larvae with each obstruction was continuously monitored for about 85 consecutive hours. Transient spatial distributions of the locations of eggs and larvae throughout the water column were generated for each flow scenario. Results show that the active swimming capabilities of larvae allow them to seek areas of low turbulence and low shear stresses, and that eggs are susceptible to damage by high levels of turbulence, which was further corroborated with tests in an oscillating grid-stirred turbulence tank. Our study seeks to better inform field collection of grass carp during early life stages, and to guide the design of alternative approaches to control the dispersal of this invasive species in North America.

Aquatic Sciences↗

USGS2018

Survival and drifting patterns of grass carp eggs and larvae in response to interactions with flow and sediment in a laboratory flume

A series of laboratory experiments was conducted to better understand the behavior of grass carp eggs and larvae in moving water in order to develop and implement new strategies for control and prediction of their dispersal and drift at early life stages. Settling velocity and density of a representative sample of eggs were estimated, and three trials of flume experiments with different flow conditions were conducted with live eggs in a temperature-controlled setting with a mobile sediment bed. In these trials, egg and larval stages were continuously analyzed over periods of 80 hours; and eggs and larvae interactions with the flow and sediment bed were monitored and characterized qualitatively and quantitatively. Survival rates were quantified after each trial, highlighting physical causes for increased mortality. Detailed flow analysis was correlated to the observed drifting and swimming behavior of eggs and larvae, to estimate distributions across the water depth, as well as traveling and swimming speeds. Evidence of the influence of mean and turbulent flow in the suspension and transport of eggs are reported, and swimming patterns of larvae at different developmental stages are described. These findings support the development of new strategies for monitoring the spread of grass carp eggs and larvae in rivers, and provide new inputs to predict conditions favorable for spawning and hatching, allowing for mitigation measures at early life stages, which are critical to control their dispersal.

PLoS ONE↗