Geology topics

Mark B. Bain

Publications and source records attributed to Mark B. Bain.

4 recordsLinked to original sources

USGS2011

Detection of Viral Hemorrhagic Septicemia Virus by Quantitative Reverse Transcription Polymerase Chain Reaction from Two Fish Species at Two Sites in Lake Superior

Viral hemorrhagic septicemia virus (VHSV) was first detected in the Laurentian Great Lakes in 2005 during a mortality event in the Bay of Quinte, Lake Ontario. Subsequent analysis of archived samples determined that the first known isolation of VHSV in the Laurentian Great Lakes was from a muskellunge Esox masquinongy collected in Lake St. Clair in 2003. By the end of 2008, mortality events and viral isolations had occurred in all of the Laurentian Great Lakes except Lake Superior. In 2009, a focused disease surveillance program was designed to determine whether VHSV was also present in Lake Superior. In this survey, 874 fish from 7 sites along the U.S. shoreline of Lake Superior were collected during June 2009. Collections were focused on nearshore species known to be susceptible to VHSV. All fish were dissected individually by using aseptic techniques and were tested for the presence of VHSV genetic material by use of a quantitative reverse transcription (qRT) polymerase chain reaction (PCR) targeting the viral nucleoprotein gene. Seventeen fish from two host species at two different sites tested positive at low levels for VHSV. All attempts to isolate virus in cell culture were unsuccessful. However, the presence of viral RNA was confirmed independently in five fish by using a nested PCR that targeted the glycoprotein (G) gene. Partial G gene sequences obtained from three fish were identical to the corresponding sequence from the original 2003 VHSV isolate (MI03) from muskellunge. These detections represent the earliest evidence for the presence of VHSV in Lake Superior and illustrate the utility of the highly sensitive qRT-PCR assay for disease surveillance in aquatic animals.

Journal of Aquatic Animal Health↗

USGS2010

Distribution of an invasive aquatic pathogen (viral hemorrhagic septicemia virus) in the Great Lakes and its relationship to shipping

Viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus found in fish from oceans of the northern hemisphere and freshwaters of Europe. It has caused extensive losses of cultured and wild fish and has become established in the North American Great Lakes. Large die-offs of wild fish in the Great Lakes due to VHSV have alarmed the public and provoked government attention on the introduction and spread of aquatic animal pathogens in freshwaters. We investigated the relations between VHSV dispersion and shipping and boating activity in the Great Lakes by sampling fish and water at sites that were commercial shipping harbors, recreational boating centers, and open shorelines. Fish and water samples were individually analyzed for VHSV using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and cell culture assays. Of 1,221 fish of 17 species, 55 were VHSV positive with highly varied qRT-PCR titers (1 to 5,950,000 N gene copies). The detections of VHSV in fish and water samples were closely associated and the virus was detected in 21 of 30 sites sampled. The occurrence of VHSV was not related to type of site or shipping related invasion hotspots. Our results indicate that VHSV is widely dispersed in the Great Lakes and is both an enzootic and epizootic pathogen. We demonstrate that pathogen distribution information could be developed quickly and is clearly needed for aquatic ecosystem conservation, management of affected populations, and informed regulation of the worldwide trade of aquatic organisms.

PLoS ONE↗

USGS1992

Interaction assessment II: A tool for population and community management

To successfully preserve or manage a species, we must understand how the community of which it is a part functions. We must know how environmental alterations affect the fitness not only of the species of interest, but also that of its competitors, predators, and prey; and we must know the form and intensity of the pertinent inter-species interactions. Interaction Assessment (INTASS) is a non-manipulative approach to constructing quantitative expressions for fitness, written as functions of measured environmental variables including local population densities of conspecifics and other interacting species. We applied this approach to, and evaluated its consistency from, data on the European land snail (Cepaea nemoralis), American eel (Anguilla rostrata), longnosed and blacknosed dace (Rhinichthys cataractae and R. atratulus), and small-mouth bass (Micropterus dolomieu) until data sets can be compiled on more typically studied vertebrates. INTASS analyses on key species of a community can provide the tools for predicting community-wide ramifications of environmental disturbances. They also can permit the planned manipulation of relative species abundances through habitat alteration.

Journal of Wildlife Management↗

USGS1982

Habitat suitability index models: Coastal stocks of striped bass

A review and synthesis of existing information were used to develop estuarine habitat models for coastal stocks of striped bass (Morone saxatilis). Models for five life stages are scaled to produce an index of habitat suitability between 0 (unsuitable habitat) and 1 (optimally suitable habitat) for estuarine areas of the continental United States. Habitat suitability indexes are designed for use with the habitat evaluation procedures developed by the U.S. Fish and Wildlife Service. Guidelines for coastal striped bass model applications and techniques for estimating model variable are described.

FWS/OBS↗