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Geology topics

K. Wolf

Publications and source records attributed to K. Wolf.

At least 55 records · Page 3Linked to original sources
USGS1978

Herpesvirus salmonis: Characterization of a new pathogen of rainbow trout

A new agent, provisionally designated Herpesvirus salmonis , was isolated from post-spawning rainbow trout ( Salmo gairdneri ) and studied primarily in the RTG-2 rainbow trout cell line. Infection of RTG-2 cells resulted in the formation of syncytia and Cowdry type A intranuclear inclusions. Replication occurred regularly at 5 and 10°C, but was inconsistent at 15°C, largely inhibited at 0°C, and completely inhibited at 20°C or higher. The virus was acid, heat, ether, and chloroform labile, but stable to freezing and thawing. It did not hemagglutinate. Viral DNA had a buoyant density of 1.709 g/cm 3 and a guanine-cytosine value of 50%. Hexagonal nucleocapsids had a diameter of 90 nm and were first seen in nuclei at 36 h. Enveloped forms measured about 150 nm and occurred both cytoplasmically and extracellularly. At 10°C, a one-step growth culture required about 96 h; cell-associated virus peaked at about 10 5 PFU/ml and exceeded released virus by a factor of about 10.

Journal of Virology↗
USGS1978

Myxosoma cerebralis: Fluorescent antibody techniques for antigen recognition

Rabbits were immunized with antigens extracted from mature spores or prespore stages of Myxosoma cerebralis , and the resulting antisera and their globulins were used in direct and indirect fluorescent antibody techniques. Both kinds of antisera reacted with homologous spores and with stages of the organism that precede spores. When tested for specificity against spores of 12 other myxosporidans the direct fluorescent antibody technique showed cross-reactivity with only one other and that was a species of Myxosoma . The indirect fluorescent antibody technique showed some reactions across generic lines. The antisera have application in studies of the parasite's life cycle and in diagnostics.

Journal of the Fisheries Research Board of Canada↗
USGS1975

Thin-section electron microscopy of mature Myxosoma cerebralis (Myxosporidea) spores

The morphology of purified, mature Myxosoma cerebralis spores was examined by thin-section electron microscopy. Surface architecture corresponded closely to that described in an earlier scanning electron microscopy study of this organism (Lom and Hoffman, 1971). A uniformly thick spore wall is composed of finely granular, electron-lucent material. Intrawall "valvoplasm" at the sutural border region contains small, light spheroidal profiles with dense centers situated in a background matrix; such structures are absent in the intermediate wall areas. Ovoid polar capsules contain complex filamentous profiles within the peripheral matrix region. Individual filaments appear twisted into S-shaped configurations and contain moderately electron-opaque material within their lumina.

Journal of Parasitology↗