Detection of Corynebacterium salmoninus by direct fluorescent antibody test
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Geology topics
Publications and source records attributed to G. L. Bullock.
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Experiments conducted simultaneously at two laboratories have shown brook trout ( Salvelinus fontinalis ) egg transmission of the virus of infectious pancreatic necrosis with eggs treated with 100 ppm iodine (as Wescodyne ® ) and with nontreated eggs.
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The bacterium causing enteric redmouth (ERM) and Aeromonas salmonicida were found to be equally pathogenic for fingerling Atlantic salmon ( Salmo salar ). Injection of 5 × 10 5 cells of ERM or A. salmonicida killed all salmon within 96 h. After a 30 min exposure to water-borne cells of the two test bacteria about one-half of the test salmon died within 14 days. Both ERM and A. salmonicida were transmitted horizontally. Results indicate that efforts should be made to prevent introduction of ERM into watersheds where Atlantic salmon occur.
A diagnostic scheme employing 10 media, 4 reagents, and 5 specific antiserums has been devised for identification of gram-negative pathogens of fishes. The scheme is not intended for detailed speciation of organisms, but for generic identification of organisms associated with infected fishes. Diagnosis can be accomplished in 24 h with selective media and antiserums, and in no more than 72 h with other media and reagents.
An indirect fluorescent antibody technique (FAT) has been developed for serological identification, diagnosis of kidney disease (KD), and detection of subclinical infections of KD. Rabbit or goat antikidney disease serum and appropriately labeled immunoglobulin was found to be specific for the KD bacterium. The technique was superior to examination of gram-stained smears for detection of small numbers of KD bacteria. The test requires approximately 2 h.
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