Search USGS⌕ Search

Geology topics

Claire J. Horwell

Publications and source records attributed to Claire J. Horwell.

18 recordsLinked to original sources
USGS2026

Getting the story straight: Common misconceptions around volcanoes and eruptions with case studies of recent events

The ability to rapidly disseminate scientific knowledge across the world has never been greater. This is paralleled by the ability of rumors to spread equally far and fast. Popular misconceptions can take root and, without correction, take on the appearance of facts. Commonly misunderstood topics include those with attention-grabbing phrasing (e.g. “Pacific Ring of Fire”, “supervolcanoes”, or “mega-tsunamis”), the intersection of volcanic research with high-profile science topics (climate or human health), aspirational science goals (eruption forecasts), and basic terminology (volcanic ash versus smoke, or magma “chambers”). In this paper, we describe eighteen misconceptions grouped in six themes (seismology, volcanoes and climate, systems and structures, volcanic hazards, timescales, photographs and footage) commonly encountered by volcanologists who are actively engaging with the public and media both during and between eruptions. Each misconception description is accompanied by takeaway points and summaries of recent research to assist the reader with the information necessary to describe the issues. Finally, we present seven case studies where incorrect information was widely shared and counteracted at well-known volcanoes and recent eruptions at Mount Agung (Bali, Indonesia), Mount Etna (Sicily, Italy), Kīlauea (Hawaii, U.S.A.), Tajogaite (La Palma, Spain), La Soufrière (St. Vincent), Taal Volcano (Philippines), and Yellowstone caldera (U.S.A.). This work is intended to help both volcanologists and non-specialists get the story straight, and to effectively communicate processes and scales of volcanism and volcanic hazards, impacts, and risks.

Journal of Applied Volcanology↗
USGS2025

The crystalline silica respiratory hazard from rhyolitic lava dome eruptions in New Zealand's Taupo Volcanic Zone: A case study from the 1315 CE Kaharoa eruption

The rhyolitic Kaharoa 1315 CE eruption was a complex, long-lived event from Tarawera volcano, New Zealand. Explosive phases were followed by around 5 years of lava dome extrusion and collapse which produced block-and-ash flows (BAF). Lava domes generate crystalline silica in the form of cristobalite, and rhyolitic magmas often contain quartz phenocrysts. Fine-grained ash containing crystalline silica can be formed through dome collapses or explosions, generating a respiratory health hazard for communities affected by ashfall. The aims of this study are to: i) determine whether the Kaharoa eruption dome-forming phase generated substantial quantities of crystalline silica and, therefore, to determine the potential for future dome-forming eruptions of Tarawera to do the same; ii) consider the potential hazard of the crystalline silica by studying the crystal habit and chemistry compared to other lava domes, globally; and iii) assess the particle size and crystalline silica content of the Kaharoa ash, to inform a respiratory hazard assessment. Five co-BAF ash samples and one co-ignimbrite (explosive) ash sample from the Kaharoa pyroclastic deposits were analysed for health-pertinent factors: particle size distribution and crystalline silica content. Eight dome-rock samples were collected from the dome complex and associated BAF deposits and groundmass texture (especially forms of crystalline silica) and quantity of crystalline silica were assessed. Cristobalite was present in the 4 ash samples analysed by X-ray diffraction (XRD; 1.3–3.7 wt%) as was quartz (5.7–12.5 wt%). For the 4 dome samples analysed by XRD, all samples contained quartz (4.1–10.4 wt%) and two contained significant quantities of cristobalite (24.7 and 27.3 wt%). Of the two dome samples with minimal cristobalite (visible as individual vapour-phase crystals by SEM but not quantifiable by XRD), one was from the non-devitrified dome carapace and the other was from the compacted interior but had not undergone devitrification. The two dome samples with substantial cristobalite were from dome interiors and were highly devitrified, with well-developed spherulitic textures. Using energy-dispersive X-ray spectroscopy, cristobalite in all samples contained minor aluminium, as has been seen for volcanic cristobalite from other lava domes, which may ameliorate its toxicity. By laser diffraction, the quantities of ash in the health pertinent size fractions varied, with a range of 1.3–8.1 vol% for particles of <4 μm diameter and 1.7–15.6 vol% for particles of <10 μm diameter, which is lower than measured in ash from large-scale dome collapse events at other volcanoes. The findings suggest a potential for substantial crystalline silica to be formed in future Kaharoa-style eruptions, but that cristobalite generation is site-specific, depending on location within the dome and whether the dome remains sufficiently hot for spherulite formation and glass devitrification. Respiratory hazard will therefore vary depending on the collapse of (or explosions through) individual lobes – although all lava is expected to contain quartz phenocrysts – as well as the size and energy of those collapses, which will influence particle size and quantity of ash generated and dispersed.

Taupo Volcanic Zone↗
USGS2023

Physicochemical hazard assessment of ash and dome rock from the 2021 eruption of La Soufrière, St Vincent, for the assessment of respiratory health impacts and water contamination

La Soufrière, St Vincent, began an extrusive eruption on 27 December 2020. The lava dome was destroyed, along with much of the pre-existing 1979 dome, in explosive eruptions from 9 to 22 April 2021. Lava domes generate crystalline silica – inhalation of which can cause silicosis in occupational settings – which can become hazardous when dome material is incorporated into volcanic ash. La Soufrière ash (17 samples) was analysed, according to IVHHN protocols, to rapidly quantify crystalline silica and test for other health-relevant properties. The basaltic andesitic ash contained <5 wt% crystalline silica, which agrees with previous analyses of ash of similar compositions and mirrors the low quantities measured in dome samples (2 area %). It contained substantial inhalable material (7–21 vol% <10 µm). Few fibre-like particles were observed, reducing concern about particle shape. Leaching assays found low concentrations of potentially toxic elements, which indicates low potential to impact health, contaminate drinking-water sources or harm grazing animals through ingestion. Collectively, these data indicate that the primary health concern from this eruption was the potential for fine-grained ash to increase ambient particulate matter, an environmental risk factor for respiratory and cardiovascular morbidity and mortality. Precautionary measures were advised to minimize exposure.

La Soufrière↗
USGS2023

Perceptions of volcanic air pollution and exposure reduction practices on the Island of Hawai‘i: Working towards socially relevant risk communication

Kīlauea volcano, on the Island of Hawai‘i, is one of the most active volcanoes in the world. Over the past four decades it has released large amounts of volcanic gases and aerosols which form volcanic air pollution known as ‘vog’. Communities downwind of Kīlauea have been chronically or episodically exposed to this potentially harmful air pollution and have raised concerns about the hazards of vog exposure. Public health and civil protection agencies have offered a range of advice, information, and mitigation strategies for living with vog. In this mixed-methods social study, we investigate the translation of official advice into practice in Island of Hawai‘i communities and assess how risk communication could be improved by considering public input, preferences, and community relevance. Given the paucity of information on the long-term effects of chronic vog exposure, assessing the effectiveness of public health and risk communication is vital.

Hawaii↗
USGS2021

Volcanic air pollution and human health: Recent advances and future directions

Volcanic air pollution from both explosive and effusive activity can affect large populations as far as thousands of kilometers away from the source, for days to decades or even centuries. Here, we summarize key advances and prospects in the assessment of health hazards, effects, risk, and management. Recent advances include standardized ash assessment methods to characterize the multiple physicochemical characteristics that might influence toxicity; the rise of community-based air quality monitoring networks using low-cost gas and particulate sensors; the development of forecasting methods for ground-level concentrations and associated public advisories; the development of risk and impact assessment methods to explore health consequences of future eruptions; and the development of evidence-based, locally specific measures for health protection. However, it remains problematic that the health effects of many major and sometimes long-duration eruptions near large populations have gone completely unmonitored. Similarly, effects of prolonged degassing on exposed populations have received very little attention relative to explosive eruptions. Furthermore, very few studies have longitudinally followed populations chronically exposed to volcanic emissions; thus, knowledge gaps remain about whether chronic exposures can trigger development of potentially fatal diseases. Instigating such studies will be facilitated by continued co-development of standardized protocols, supporting local study teams and procuring equipment, funding, and ethical permissions. Relationship building between visiting researchers and host country academic, observatory, and agency partners is vital and can, in turn, support the effective communication of health impacts of volcanic air pollution to populations, health practitioners, and emergency managers.

Bulletin of Volcanology↗
USGS2021

Assessing the biological reactivity of organic compounds on volcanic ash: Implications for human health hazard

Exposure to volcanic ash is a long-standing health concern for people living near active volcanoes and in distal urban areas. During transport and deposition, ash is subjected to various physicochemical processes that may change its surface composition and, consequently, bioreactivity. One such process is the interaction with anthropogenic pollutants; however, the potential for adsorbed, deleterious organic compounds to directly impact human health is unknown. We use an in vitro bioanalytical approach to screen for the presence of organic compounds of toxicological concern on ash surfaces and assess their biological potency. These compounds include polycyclic aromatic hydrocarbons (PAHs), polychlorinated dibenzo- p -dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dlPCBs). Analysis of ash collected in or near urbanised areas at five active volcanoes across the world (Etna, Italy; Fuego, Guatemala; Kelud, Indonesia; Sakurajima, Japan; Tungurahua, Ecuador) using the bioassay inferred the presence of such compounds on all samples. A relatively low response to PCDD/Fs and the absence of a dlPCBs response in the bioassay suggest that the measured activity is dominated by PAHs and PAH-like compounds. This study is the first to demonstrate a biological potency of organic pollutants associated with volcanic ash particles. According to our estimations, they are present in quantities below recommended exposure limits and likely pose a low direct concern for human health.

Bulletin of Volcanology↗
USGS2021

Development of a simulated lung fluid leaching method to assess the release of potentially toxic elements from volcanic ash

Freshly erupted volcanic ash contains a range of soluble elements, some of which can generate harmful effects in living cells and are considered potentially toxic elements (PTEs). This work investigates the leaching dynamics of ash-associated PTEs in order to optimize a method for volcanic ash respiratory hazard assessment. Using three pristine (unaffected by precipitation) ash samples, we quantify the release of PTEs (Al, Cd, Co, Cr, Cu, Fe, Mn, Ni, Pb, V, Zn) and major cations typical of ash leachates (Mg, Na, Ca, K) in multiple simulated lung fluid (SLF) preparations and under varying experimental parameters (contact time and solid to liquid ratio). Data are compared to a standard water leach (WL) to ascertain whether the WL can be used as a simple proxy for SLF leaching. The main findings are: PTE concentrations reach steady-state dissolution by 24 h, and a relatively short contact time (10 min) approximates maximum dissolution; PTE dissolution is comparatively stable at low solid to liquid ratios (1:100 to 1:1000); inclusion of commonly used macromolecules has element-specific effects, and addition of a lung surfactant has little impact on extraction efficiency. These observations indicate that a WL can be used to approximate lung bioaccessible PTEs in an eruption response situation. This is a useful step towards standardizing in vitro methods to determine the soluble-element hazard from inhaled ash.

Chemosphere↗
USGS2021

Report on geochemical characterisation of volcanic ash from Cumbre Vieja, La Palma, for the assessment of respiratory health hazard

Volcanic ash has several hazardous characteristics that may impact human and animal health. We report the results of a geochemical assessment of key hazardous characteristics of 16 volcanic ash samples erupted from Cumbre Vieja, La Palma, between 19 September – 16 October 2021. The analyses are an essential first step in assessing health risk from volcanic eruptions because they allow a rapid screening of the physicochemical characteristics that have the potential to cause harm. The report is in two sections: Part 1 presents analyses related to respiratory hazard, while Part 2 presents analyses relevant for contamination of drinking water and ingestion hazards for livestock. The main authors (Horwell, Damby & Stewart) are directors of the International Volcanic Health Hazard Network (www.ivhhn.org) and have conducted and published extensive research to develop and test techniques for rapid analysis of volcanic ash for health hazard assessment.

Cumbre Vieja, La Palma↗
USGS2020

Assessment of leachable elements in volcanic ashfall: A review and evaluation of a standardized protocol for ash hazard characterization

Volcanic ash presents a widespread and common hazard during and after eruptions. Complex interactions between solid ash surfaces and volcanic gases lead to the formation of soluble salts that may be mobilized in aqueous environments. A variety of stakeholders may be concerned about the effects of ash on human and animal health, drinking water supplies, crops, soils and surface runoff. As part of the immediate emergency response, rapid dissemination of information regarding potentially hazardous concentrations of soluble species is critical. However, substantial variability in the methods used to characterize leachable elements makes it challenging to compare datasets and eruption impacts. To address these challenges, the International Volcanic Health Hazard Network ( www.ivhhn.org ) organized a two-day workshop to define appropriate methods for hazard assessment. The outcome of this workshop was a ‘consensus protocol’ for analysis of volcanic ash samples for rapid assessment of hazards from leachable elements, which was subsequently ratified by leading volcanological organizations. The purpose of this protocol is to recommend clear, standard and reliable methods applicable to a range of purposes during eruption response, such as assessing impacts on drinking-water supplies and ingestion hazards to livestock, and also applicable to research purposes. Where possible, it is intended that the methods make use of commonly available equipment and require little training. To evaluate method transferability, an interlaboratory comparison exercise was organized among six laboratories worldwide. Each laboratory received a split of pristine ash, and independently analyzed it according to the protocol for a wide range of elements. Collated results indicate good repeatability and reproducibility for most elements, thus indicating that the development of this protocol is a useful step towards providing standardized and reliable methods for ash hazard characterization. In this article, we review recent ash leachate studies, report the outcomes of the comparison exercise and present a revised and updated protocol based on the experiences and recommendations of the exercise participants. The adoption of standardized methods will improve and facilitate the comparability of results among studies and enable the ongoing development of a global database of leachate information relevant for informing volcanic health hazards assessment.

Journal of Volcanology and Geothermal Research↗
USGS2020

The health hazards of volcanic and geothermal gases: A guide for the public

This guide has been prepared by the International Volcanic Health Hazard Network (IVHHN) to explain the potential adverse health effects of volcanic and geothermal gas and aerosol emissions. It provides general information on how to protect yourself and your family. The information is based on a range of evidence from academics and health and regulatory agencies. Contact your local public health or emergency management agency for information tailored to your location.

Report↗
USGS2019

Assessment of the potential for in-plume sulphur dioxide gas-ash interactions to influence the respiratory toxicity of volcanic ash

Background Volcanic plumes are complex environments composed of gases and ash particles, where chemical and physical processes occur at different temperature and compositional regimes. Commonly, soluble sulphate- and chloride-bearing salts are formed on ash as gases interact with ash surfaces. Exposure to respirable volcanic ash following an eruption is potentially a significant health concern. The impact of such gas-ash interactions on ash toxicity is wholly un-investigated. Here, we study, for the first time, whether the interaction of volcanic particles with sulphur dioxide (SO 2 ) gas, and the resulting presence of sulphate salt deposits on particle surfaces, influences toxicity to the respiratory system, using an advanced in vitro approach. Methods To emplace surface sulphate salts on particles, via replication of the physicochemical reactions that occur between pristine ash surfaces and volcanic gas, analogue substrates (powdered synthetic volcanic glass and natural pumice) were exposed to SO 2 at 500 °C, in a novel Advanced Gas-Ash Reactor, resulting in salt-laden particles. The solubility of surface salt deposits was then assessed by leaching in water and geochemical modelling. A human multicellular lung model was exposed to aerosolised salt-laden and pristine (salt-free) particles, and incubated for 24 h. Cell cultures were subsequently assessed for biological endpoints, including cytotoxicity (lactate dehydrogenase release), oxidative stress (oxidative stress-related gene expression; heme oxygenase 1 and NAD(P)H dehydrogenase [quinone] 1) and its (pro-)inflammatory response (tumour necrosis factor α, interleukin 8 and interleukin 1β at gene and protein levels). Results In the lung cell model no significant effects were observed between the pristine and SO 2 -exposed particles, indicating that the surface salt deposits, and the underlying alterations to the substrate, do not cause acute adverse effects in vitro . Based on the leachate data, the majority of the sulphate salts from the ash surfaces are likely to dissolve in the lungs prior to cellular uptake. Conclusions The findings of this study indicate that interaction of volcanic ash with SO 2 during ash generation and transport does not significantly affect the respiratory toxicity of volcanic ash in vitro . Therefore, sulphate salts are unlikely a dominant factor controlling variability in in vitro toxicity assessments observed during previous eruption response efforts.

Environmental Research↗
USGS2018

Volcanic ash activates the NLRP3 inflammasome in murine and human macrophages

Volcanic ash is a heterogeneous mineral dust that is typically composed of a mixture of amorphous (glass) and crystalline (mineral) fragments. It commonly contains an abundance of the crystalline silica (SiO 2 ) polymorph cristobalite. Inhalation of crystalline silica can induce inflammation by stimulating the NLRP3 inflammasome, a cytosolic receptor complex that plays a critical role in driving inflammatory immune responses. Ingested material results in the assembly of NLRP3, ASC, and caspase-1 with subsequent secretion of the interleukin-1 family cytokine IL-1β. Previous toxicology work suggests that cristobalite-bearing volcanic ash is minimally reactive, calling into question the reactivity of volcanically derived crystalline silica, in general. In this study, we target the NLRP3 inflammasome as a crystalline silica responsive element to clarify volcanic cristobalite reactivity. We expose immortalized bone marrow-derived macrophages of genetically engineered mice and primary human peripheral blood mononuclear cells (PBMCs) to ash from the Soufrière Hills volcano as well as representative, pure-phase samples of its primary componentry (volcanic glass, feldspar, cristobalite) and measure NLRP3 inflammasome activation. We demonstrate that respirable Soufrière Hills volcanic ash induces the activation of caspase-1 with subsequent release of mature IL-1β in a NLRP3 inflammasome-dependent manner. Macrophages deficient in NLRP3 inflammasome components are incapable of secreting IL-1β in response to volcanic ash ingestion. Cellular uptake induces lysosomal destabilization involving cysteine proteases. Furthermore, the response involves activation of mitochondrial stress pathways leading to the generation of reactive oxygen species. Considering ash componentry, cristobalite is the most reactive pure-phase with other components inducing only low-level IL-1β secretion. Inflammasome activation mediated by inhaled ash and its potential relevance in chronic pulmonary disease was further evidenced in PBMC using the NLRP3 small-molecule inhibitor CP-456,773 (CRID3, MCC950). Our data indicate the functional activation of the NLRP3 inflammasome by volcanic ash in murine and human macrophages in vitro . Cristobalite is identified as the apparent driver, thereby contesting previous assertions that chemical and structural imperfections may be sufficient to abrogate the reactivity of volcanically derived cristobalite. This is a novel mechanism for the stimulation of a pro-inflammatory response by volcanic particulate and provides new insight regarding chronic exposure to environmentally occurring particles.

Frontiers in Immunology↗
USGS2018

Respiratory hazard assessment of combined exposure to complete gasoline exhaust and respirable volcanic ash in a multicellular human lung model at the air-liquid interface

Communities resident in urban areas located near active volcanoes can experience volcanic ash exposures during, and following, an eruption, in addition to sustained exposures to high concentrations of anthropogenic air pollutants ( e.g., vehicle exhaust emissions). Inhalation of anthropogenic pollution is known to cause the onset of, or exacerbate, respiratory and cardiovascular diseases. It is further postulated similar exposure to volcanic ash can also affect such disease states. Understanding of the impact of combined exposure of volcanic ash and anthropogenic pollution to human health, however, remains limited. The aim of this study was to assess the biological impact of combined exposure to respirable volcanic ash (from Soufrière Hills volcano (SHV), Montserrat and Chaitén volcano (ChV), Chile; representing different magmatic compositions and eruption styles) and freshly-generated complete exhaust from a gasoline vehicle. A multicellular human lung model (an epithelial cell-layer composed of A549 alveolar type II-like cells complemented with human blood monocyte-derived macrophages and dendritic cells cultured at the air-liquid interface) was exposed to diluted exhaust (1:10) continuously for 6 h, followed by immediate exposure to the ash as a dry powder (0.54 ± 0.19 μg/cm 2 and 0.39 ± 0.09 μg/cm 2 for SHV and ChV ash, respectively). After an 18 h incubation, cells were exposed again for 6 h to diluted exhaust, and a final 18 h incubation (at 37 °C and 5% CO 2 ). Cell cultures were then assessed for cytotoxic, oxidative stress and (pro-)inflammatory responses. Results indicate that, at all tested (sub-lethal) concentrations, co-exposures with both ash samples induced no significant expression of genes associated with oxidative stress ( HMOX1, NQO1 ) or production of (pro-)inflammatory markers (IL-1β, IL-8, TNF-α) at the gene and protein levels. In summary, considering the employed experimental conditions, combined exposure of volcanic ash and gasoline vehicle exhaust has a limited short-term biological impact to an advanced lung cell in vitro model.

Environmental Pollution↗
USGS2017

Assessment of the potential respiratory hazard of volcanic ash from future Icelandic eruptions: A study of archived basaltic to rhyolitic ash samples

Background The eruptions of Eyjafjallajökull (2010) and Grímsvötn (2011), Iceland, triggered immediate, international consideration of the respiratory health hazard of inhaling volcanic ash, and prompted the need to estimate the potential hazard posed by future eruptions of Iceland’s volcanoes to Icelandic and Northern European populations. Methods A physicochemical characterization and toxicological assessment was conducted on a suite of archived ash samples spanning the spectrum of past eruptions (basaltic to rhyolitic magmatic composition) of Icelandic volcanoes following a protocol specifically designed by the International Volcanic Health Hazard Network. Results Icelandic ash can be of a respirable size (up to 11.3 vol.% < 4 μm), but the samples did not display physicochemical characteristics of pathogenic particulate in terms of composition or morphology. Ash particles were generally angular, being composed of fragmented glass and crystals. Few fiber-like particles were observed, but those present comprised glass or sodium oxides, and are not related to pathogenic natural fibers, like asbestos or fibrous zeolites, thereby limiting concern of associated respiratory diseases. None of the samples contained cristobalite or tridymite, and only one sample contained quartz, minerals of interest due to the potential to cause silicosis. Sample surface areas are low, ranging from 0.4 to 1.6 m2 g−1, which aligns with analyses on ash from other eruptions worldwide. All samples generated a low level of hydroxyl radicals (HO•), a measure of surface reactivity, through the iron-catalyzed Fenton reaction compared to concurrently analyzed comparative samples. However, radical generation increased after ‘refreshing’ sample surfaces, indicating that newly erupted samples may display higher reactivity. A composition-dependent range of available surface iron was measured after a 7-day incubation, from 22.5 to 315.7 μmol m−2, with mafic samples releasing more iron than silicic samples. All samples were non-reactive in a test of red blood cell-membrane damage. Conclusions The primary particle-specific concern is the potential for future eruptions of Iceland’s volcanoes to generate fine, respirable material and, thus, to increase ambient PM concentrations. This particularly applies to highly explosive silicic eruptions, but can also hold true for explosive basaltic eruptions or discrete events associated with basaltic fissure eruptions.

Environmental Health↗
USGS2017

The effect of aluminium and sodium impurities on the in vitro toxicity and pro-inflammatory potential of cristobalite

Background Exposure to crystalline silica (SiO 2 ), in the form of quartz, tridymite or cristobalite, can cause respiratory diseases, such as silicosis. However, the observed toxicity and pathogenicity of crystalline silica is highly variable. This has been attributed to a number of inherent and external factors, including the presence of impurities. In cristobalite-rich dusts, substitutions of aluminium (Al) for silicon (Si) in the cristobalite structure, and impurities occluding the silica surface, have been hypothesised to decrease its toxicity. This hypothesis is tested here through the characterisation and in vitro toxicological study of synthesised cristobalite with incremental amounts of Al and sodium (Na) dopants. Methods Samples of synthetic cristobalite with incremental amounts of Al and Na impurities, and tridymite, were produced through heating of a silica sol-gel. Samples were characterised for mineralogy, cristobalite purity and abundance, particle size, surface area and surface charge. In vitro assays assessed the ability of the samples to induce cytotoxicity and TNF-α production in J774 macrophages, and haemolysis of red blood cells. Results Al-only doped or Al+Na co-doped cristobalite contained between 1 and 4 oxide wt% Al and Na within its structure. Co-doped samples also contained Al- and Na-rich phases, such as albite. Doping reduced cytotoxicity to J774 macrophages and haemolytic capacity compared to non-doped samples. Al-only doping was more effective at decreasing cristobalite reactivity than Al+Na co-doping. The reduction in the reactivity of cristobalite is attributed to both structural impurities and a lower abundance of crystalline silica in doped samples. Neither non-doped nor doped crystalline silica induced production of the pro-inflammatory cytokine TNF-α in J774 macrophages. Conclusions Impurities can reduce the toxic potential of cristobalite and may help explain the low reactivity of some cristobalite-rich dusts. Whilst further work is required to determine if these effects translate to altered pathogenesis, the results have potential implications for the regulation of crystalline silica exposures.

Environmental Research↗
USGS2016

Combined exposure of diesel exhaust particles and respirable Soufrière Hills volcanic ash causes a (pro-)inflammatory response in an in vitro multicellular epithelial tissue barrier model

Background There are justifiable health concerns regarding the potential adverse effects associated with human exposure to volcanic ash (VA) particles, especially when considering communities living in urban areas already exposed to heightened air pollution. The aim of this study was, therefore, to gain an imperative, first understanding of the biological impacts of respirable VA when exposed concomitantly with diesel particles. Methods A sophisticated in vitro 3D triple cell co-culture model of the human alveolar epithelial tissue barrier was exposed to either a single or repeated dose of dry respirable VA (deposited dose of 0.26 ± 0.09 or 0.89 ± 0.29 μg/cm 2 , respectively) from Soufrière Hills volcano, Montserrat for a period of 24 h at the air-liquid interface (ALI). Subsequently, co-cultures were exposed to co-exposures of single or repeated VA and diesel exhaust particles (DEP; NIST SRM 2975; 0.02 mg/mL), a model urban pollutant, at the pseudo-ALI. The biological impact of each individual particle type was also analysed under these precise scenarios. The cytotoxic (LDH release), oxidative stress (depletion of intracellular GSH) and (pro-)inflammatory (TNF-α, IL-8 and IL-1β) responses were assessed after the particulate exposures. The impact of VA exposure upon cell morphology, as well as its interaction with the multicellular model, was visualised via confocal laser scanning microscopy (LSM) and scanning electron microscopy (SEM), respectively. Results The combination of respirable VA and DEP, in all scenarios, incited an heightened release of TNF-α and IL-8 as well as significant increases in IL-1β, when applied at sub-lethal doses to the co-culture compared to VA exposure alone. Notably, the augmented (pro-)inflammatory responses observed were not mediated by oxidative stress. LSM supported the quantitative assessment of cytotoxicity, with no changes in cell morphology within the barrier model evident. A direct interaction of the VA with all three cell types of the multicellular system was observed by SEM. Conclusions Combined exposure of respirable Soufrière Hills VA with DEP causes a (pro-)inflammatory effect in an advanced in vitro multicellular model of the epithelial airway barrier. This finding suggests that the combined exposure to volcanic and urban particulate matter should be further investigated in order to deduce the potential human health hazard, especially how it may influence the respiratory function of susceptible individuals (i.e. with pre-existing lung diseases) in the population.

Particle and Fibre Toxicology↗