Geology topics

Bruce W. Lium

Publications and source records attributed to Bruce W. Lium.

6 recordsLinked to original sources

USGS1977

Limnological studies of the major streams in Chester County, Pennsylvania

The major streams in Chester County, Pennsylvania were studied to determine their environmental state, and to relate this condition to land- use patterns. A biological numerical rating system on a scale of 1 to 10 was developed to classify stream conditions. One indicates extremely toxic conditions and 10 balanced biological conditions. The majority of the streams studied had ratings between 4 and 8, indicating they were experiencing varying degrees of enrichment and/or sediment deposition. The streams with high numerical ratings were generally found in rural and agricultural areas. Streams with low numerical rating were found mostly in areas near waste treatment facilities or industry. Large changes in the numerical rating of a stream were related to changes in land-use patterns. Relationships were developed between dissolved solids concentrations and the biological numerical ratings of the streams. Streams with ratings from 6 to 10 had dissolved solids concentrations of approximately 100 mg/L. At ratings of 5 to 1, the dissolved solids concentrations increased progressively from 122 to 310 mg/L. Chemical data for the streams were related to the dominant orders of benthic invertebrates. The stoneflies were found to be the most sensitive to high levels of chemical constituents. The trueflies, mayflies, and caddis flies showed a wide tolerance to various chemical constituents.

Pennsylvania↗

USGS1977

The quantitative determination of chlorophylls A and B from freshwater algae without interference from degradation products

Chlorophyll was efficiently extracted from freshwater algae with dimethyl sulfoxide. The extract was chromatographed on commercially available thin-layer cellulose sheets, 2 percent methanol and 98 percent petroleum ether being used as the solvent. Chlorophylls a and b , and phaeophytins a and b were cleanly separated from each other and other degradation products. No artifacts were formed. Recoveries of chlorophylls a and b were 98 and 96 percent as measured by spectrophotometry or fluorometry.

Journal of Research of the U.S. Geological Survey↗

USGS1976

Limnological data for the major streams in Chester County, Pennsylvania

Limnological data on major streams in Chester County, Pennsylvania are tabulated to provide a base line as to the present stream conditions. As land-use patterns change and further urbanization takes place, it is anticipated that these data will serve as a basis for comparison of conditions in the future. The 13 basins encompass a total drainage area of 1,812 km2 (697 mi2) of the 1,976 km2 (760 mi2) within Chester County. Four of the streams are tributaries to the Schuylkill River and include Pickering, Stony Run, Pigeon, and French Creek. Tributaries to the Delaware River include Darby, Crum, Ridley, Chester, Red Clay, and White Clay Creeks. The Elk Creeks flow into the Chesapeake Bay. The Brandywine is a tributary to the Christiana River, itself a tributary to the Delaware. The Octoraro Creek is a tributary to the Susquehanna River. Chester County, which is located in the Piedmont area of Pennsylvania, is undergoing rapid transition from a rural to suburban condition as the population increases. This basic-data report contains the chemical, physical, and biological information collected for the period fall 1969 through fall 1974. The network of 50 stations is shown in figure 1, which is followed by a listing (table 1) of station numbers and a brief description of station locations. Table 2 shows the physical, chemical, and bacteriological data collected by station and date. Table 3 shows the benthic invertebrate data collected by station and date.

Pennsylvania↗

USGS1976

The measurement of adenosine triphosphate in pure algal cultures and natural aquatic samples

Three methods for the extraction of adenosine triphosphate (ATP) neutral dimethyl sulfoxide (DMSO), boiling tris buffer, and butanol-octanol extraction were equally effective on the alga Chlorella vulgwia . Dilution of extracted ATP samples was linear. Filtration of different volumes of samples resulted in proportional values for ATP in the extracts. Measurement of activity by either peak height or integration of the area under the peak were equally sensitive and reproducible. The assay of ATP sample was inhibited by mercuric chloride > cadmium chloride > calcium chloride > potassium or sodium phosphate, and by high concentrations of the extractant DMSO. Analysis of ATP in aquatic environments led to the problem of transferring a sample from the field to the laboratory without obtaining a change in ATP concentration. Membrane filtration of the sample followed by chilling at 4°C, slow freezing at 20° C, or freezing on dry ice were ineffective in maintaining a constant level of ATP. Chilling caused a marked increase in ATP, whereas slow freezing caused a significant loss of ATP. Freezing on dry ice was variable but generally resulted in large losses of ATP. Quick freezing by immersion of filter and algae in liquid nitrogen and storage on dry ice maintained a constant ATP level. Field extraction of the ATP followed by quick freezing in an acetone-dry-ice bath maintained the ATP in a convenient and stable form.

Journal of Research of the U.S. Geological Survey↗

USGS1976

An evaluation of methods for measuring algal growth

Algal growth was expressed as milligrams dry weight per litre with use of the electronic particle counter for determining cell number and cell volume. A gravimetric constant in milligrams dry weight per cubic micrometre was determined. This constant was applicable throughout the growth curve since there was a linear relationship between total cell weight and total cell volume. Cell volume must be considered in measuring growth because there is a change in average cell volume in addition to an increase in cell numbers throughout the growth curve. Chlorella vulgaris and Selenastrum capricornutum exhibited similar growth curves when growth was expressed as cells per litre or as milligrams dry weight per litre. Chlamydomonas reinhardtii appeared to have a lower algal growth potential when measured in cells per litre but had the same growth potential as C. vulgaris and S. capricornutum when expressed as milligrams dry weight per litre. The growth rate for Scenedesmus quadricauda was less than the other three, regardless of how the results were expressed. The image analysis system was used to count different types of algae in a mixed culture. C. reinhardtii , C. vulgaris , and Anacystis nidulans were not grossly distorted by the sample preparation method and could be distinguished from one another by variations in morphology. The counts could be separated by computing the ratio of area to length for each particle and by allowing the computer to sort and count each of the three types. Typical ratios ranged from 5 to 7 for G. reinhardtii , 2 to 4 C. vulgaris , and 0.2 to 1.3 for A. nidulans . The number of types to be counted in a mixture was limited only by the ability to apply shape factors without having an overlap between each type. Both the image analysis and electronic particle counter produced equal count and size measurements. Fluorescence was a sensitive measure of growth on a laboratory prepared medium since there was a logarithmic relationship between fluorescence and cells per millilitre for both C. vulgaris and S. capricornutum . Fluorescence measurements were not satisfactory for determining growth in samples of varying chemical composition (which occurs in natural samples) because the chlorophyll-to-cell ratio varied with media composition. This was especially pronounced with C. vulgaris .

Journal of Research of the U.S. Geological Survey↗

USGS1974

Some aspects of aquatic insect populations of pools and riffles in gravel bed streams in western United States

Relationships were studied between aquatic-insect populations on rocks of different sizes and variations of populations between different stream localities. All aquatic insects were counted and identified on streambed gravels and cobbles chosen at random from four rivers on three to four types of locations ranging from riffles to pools. The measurements used span 3 to 4 months in 1 year. Insect density (number of insects per unit of rock area) was determined to be a function of rock size. Highest insect densities occurred on rocks between 45 and 90 mm mean diameter, with larger and smaller rocks having lower insect densities. The insect population differed significantly with location within a stream; that is, from pool to riffle, as well as by stream and season.

Journal of Research of the U.S. Geological Survey↗