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Geology topics

B. C. Lidgerding

Publications and source records attributed to B. C. Lidgerding.

11 recordsLinked to original sources
USGS2006

Mycoplasma contamination in fish cell lines: An evaluation of detection by differential incorporation of 3H-uridine and 14C-uracil

Differential incorporation of uridine and uracil was used to assay for mycoplasma contamination in five fish cell lines: bluegill fry (BF-2), chinook salmon embryo (CHSE-214), epithelioma papillosum cyprini (EPC), fathead minnow (FHM) and rainbow trout gonad (RTG-2). The method was not suitable for monitoring BF-2, CHSE-214, FHM, and RTG-2 cell lines because they incorporated uracil. Differential incorporation of uridine and uracil may be applicable for screening EPC cells because only this cell line could distinguish cultures experimentally infected with Mycoplasma orale from cultures known to be free from microbial contaminants.

Journal of Fish Diseases↗
USGS1985

Production and characterization of monoclonal antibody against infectious hematopoietic necrosis virus

A hybridoma cell line that continually secretes antibody against infectious hematopoietic necrosis virus was developed and designated M-IHNV-W1. Antibodies in culture fluids were concentrated and purified by Protein A Sepharose CL-4B affinity chromatography. The immunoglobulin was isotyped as IgG 2b with a kappa light chain. The IgG was specific for infectious hematopoietic necrosis virus and had binding activity but low neutralizing activity. Little cross-reactivity occurred with viral hemorrhagic septicemia or infectious pancreatic necrosis viruses.

Fish Pathology↗
USGS1985

Viral diseases of fish: first report of carp pox in golden ide (Leuciscus idus) in North America

Carp pox, a putative viral disease exotic to North America, occurred in golden ide 1 yr after the fish were imported into the United States from the Federal Republic of Germany. The raised, white, plaque-like lesions, which occurred on about 5% of the fish, healed spontaneously and caused no mortality. Electron micrographs showed herpesvirus-like particles associated with lesion specimens; however, no infectious viruses were detected in tests with seven warmwater fish cell lines.

Journal of Wildlife Diseases↗
USGS1984

Fish cell lines: Characterization by isozyme analysis

The electrophoretic mobilities of isozymes from 16 enzyme systems were determined for nine fish cell lines. The relative migration of the malate dehydrogenase and 6-phosphoglucose dehydrogenase isozymes could be used together to distinguish between seven of the fish cell lines. Two cyprinid cell lines could not be distinguished from each other but were readily separated from the six noncyprinid lines and the one other line of cyprinid origin.

In Vitro↗